Rh1DG404100

Carboxylesterase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0000860
Physical Location & Seq
Reverse (-)
62352163 .. 62352462
300 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG404100.1

Sequence Viewer

Length: 300 bp
ATGTGGGACTTGTCGTTGCCTATCGGGGCTGACCGTGATCACGAGTATTGCAATCCAATGGCGGGTGGCGATTCTAATGGCTTTGATCAAGTGAAAGCACTTGGGTGGAAAGTTTTGGTGAGTGGCTCCGATGGAGACCCGCTGATCGATCGTCAGATTGAGCTGGTGAAGATGTTGGAGGGGAAGGGTGTACATGTGGTGAGTCAGTTTATTGAAGGAGGTTATCATGGGGCTCAAGATGTTGAACCCTCCAAAGCCAAGGATGTGTTTGTGCTTGTTAAACATTTCATATTTCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0009056 GO:0016787
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

99

Amino Acids

10.85

Weight (kDa)

4.95

Isoelectric Point (pI)

30.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydrolase_3 PF07859 1 - 78 8.1e-09 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 62, 140
AfaI GTAC 1 cut(s) 192
AfiI CCNNNNNNNGG 1 cut(s) 62
AflIII ACRYGT 1 cut(s) 193
AgsI TTSAA 2 cut(s) 215, 245
AleI CACNNNNGTG 1 cut(s) 103
AluBI AGCT 1 cut(s) 163
AluI AGCT 1 cut(s) 163
Alw26I GTCTC 1 cut(s) 129
AsuHPI GGTGA 3 cut(s) 130, 178, 211
BanII GRGCYC 1 cut(s) 235
BauI CACGAG 1 cut(s) 41
BccI CCATC 1 cut(s) 125
BclI TGATCA 2 cut(s) 37, 85
BcoDI GTCTC 1 cut(s) 129
BmiI GGNNCC 1 cut(s) 127
BpuEI CTTGAG 1 cut(s) 219
Bsa29I ATCGAT 1 cut(s) 147
BsaI GGTCTC 1 cut(s) 129
BsaJI CCNNGG 1 cut(s) 258
Bsc4I CCNNNNNNNGG 1 cut(s) 62
BseCI ATCGAT 1 cut(s) 147
BseDI CCNNGG 1 cut(s) 258
BseGI GGATG 1 cut(s) 268
BseLI CCNNNNNNNGG 1 cut(s) 62
Bsh1285I CGRYCG 1 cut(s) 151
BshVI ATCGAT 1 cut(s) 147
BsiEI CGRYCG 1 cut(s) 151
BslFI GGGAC 1 cut(s) 20
BslI CCNNNNNNNGG 1 cut(s) 62
BsmAI GTCTC 1 cut(s) 129
BsmFI GGGAC 1 cut(s) 20
Bso31I GGTCTC 1 cut(s) 129
Bsp1286I GDGCHC 1 cut(s) 235
Bsp1407I TGTACA 1 cut(s) 190
Bsp143I GATC 4 cut(s) 37, 85, 144, 148
BspACI CCGC 2 cut(s) 62, 140
BspDI ATCGAT 1 cut(s) 147
BspLI GGNNCC 1 cut(s) 127
BspTNI GGTCTC 1 cut(s) 129
BsrGI TGTACA 1 cut(s) 190
BssECI CCNNGG 1 cut(s) 258
BssMI GATC 4 cut(s) 37, 85, 144, 148
BssSI CACGAG 1 cut(s) 41
BssT1I CCWWGG 1 cut(s) 258
Bst2BI CACGAG 1 cut(s) 41
Bst4CI ACNGT 1 cut(s) 35
BstAUI TGTACA 1 cut(s) 190
BstF5I GGATG 1 cut(s) 268
BstKTI GATC 4 cut(s) 40, 88, 147, 151
BstMAI GTCTC 1 cut(s) 129
BstMBI GATC 4 cut(s) 37, 85, 144, 148
BstMCI CGRYCG 1 cut(s) 151
BstNSI RCATGY 1 cut(s) 197
Bsu15I ATCGAT 1 cut(s) 147
BsuTUI ATCGAT 1 cut(s) 147
BtsCI GGATG 1 cut(s) 268
ClaI ATCGAT 1 cut(s) 147
Csp6I GTAC 1 cut(s) 191
CviAII CATG 2 cut(s) 194, 227
CviJI RGCY 6 cut(s) 29, 81, 126, 163, 233, 257
CviKI_1 RGCY 6 cut(s) 29, 81, 126, 163, 233, 257
CviQI GTAC 1 cut(s) 191
DpnI GATC 4 cut(s) 39, 87, 146, 150
DpnII GATC 4 cut(s) 37, 85, 144, 148
Eco130I CCWWGG 1 cut(s) 258
Eco24I GRGCYC 1 cut(s) 235
Eco31I GGTCTC 1 cut(s) 129
EcoT14I CCWWGG 1 cut(s) 258
EcoT38I GRGCYC 1 cut(s) 235
ErhI CCWWGG 1 cut(s) 258
FaeI CATG 2 cut(s) 197, 230
FaiI YATR 3 cut(s) 195, 228, 290
FaqI GGGAC 1 cut(s) 20
FatI CATG 2 cut(s) 193, 226
FauI CCCGC 2 cut(s) 55, 147
FbaI TGATCA 2 cut(s) 37, 85
FokI GGATG 1 cut(s) 275
FriOI GRGCYC 1 cut(s) 235
Hin1II CATG 2 cut(s) 197, 230
HinfI GANTC 2 cut(s) 71, 202
HphI GGTGA 3 cut(s) 130, 178, 211
Hpy166II GTNNAC 1 cut(s) 191
Hpy188I TCNGA 2 cut(s) 130, 156
Hpy188III TCNNGA 2 cut(s) 41, 236
Hpy8I GTNNAC 1 cut(s) 191
HpyAV CCTTC 2 cut(s) 178, 209
HpyCH4III ACNGT 1 cut(s) 35
HpyCH4V TGCA 1 cut(s) 51
Hsp92II CATG 2 cut(s) 197, 230
Ksp22I TGATCA 2 cut(s) 37, 85
Kzo9I GATC 4 cut(s) 37, 85, 144, 148
LmnI GCTCC 1 cut(s) 131
LpnPI CCDG 1 cut(s) 149
MalI GATC 4 cut(s) 39, 87, 146, 150
MboI GATC 4 cut(s) 37, 85, 144, 148
MboII GAAGA 1 cut(s) 181
MhlI GDGCHC 1 cut(s) 235
MlyI GAGTC 1 cut(s) 211
MmeI TCCRAC 1 cut(s) 156
MnlI CCTC 3 cut(s) 172, 212, 259
MseI TTAA 2 cut(s) 279, 298
MslI CAYNNNNRTG 1 cut(s) 103
MspA1I CMGCKG 1 cut(s) 142
NdeII GATC 4 cut(s) 37, 85, 144, 148
NlaIII CATG 2 cut(s) 197, 230
NlaIV GGNNCC 1 cut(s) 127
NspI RCATGY 1 cut(s) 197
OliI CACNNNNGTG 1 cut(s) 103
PciI ACATGT 1 cut(s) 193
PfeI GAWTC 1 cut(s) 71
Ple19I CGATCG 1 cut(s) 151
PleI GAGTC 1 cut(s) 210
PpsI GAGTC 1 cut(s) 210
PscI ACATGT 1 cut(s) 193
PspN4I GGNNCC 1 cut(s) 127
PvuI CGATCG 1 cut(s) 151
RsaI GTAC 1 cut(s) 192
RsaNI GTAC 1 cut(s) 191
RseI CAYNNNNRTG 1 cut(s) 103
SaqAI TTAA 2 cut(s) 279, 298
Sau3AI GATC 4 cut(s) 37, 85, 144, 148
SchI GAGTC 1 cut(s) 211
SduI GDGCHC 1 cut(s) 235
SetI ASST 2 cut(s) 165, 223
SmiMI CAYNNNNRTG 1 cut(s) 103
SmlI CTYRAG 1 cut(s) 234
SmoI CTYRAG 1 cut(s) 234
SsiI CCGC 2 cut(s) 62, 140
StyI CCWWGG 1 cut(s) 258
TaaI ACNGT 1 cut(s) 35
TaqI TCGA 1 cut(s) 147
TatI WGTACW 1 cut(s) 190
TfiI GAWTC 1 cut(s) 71
Tru1I TTAA 2 cut(s) 279, 298
Tru9I TTAA 2 cut(s) 279, 298
TspDTI ATGAA 1 cut(s) 277
XceI RCATGY 1 cut(s) 197
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.