Rroxscaffold_3G00264170

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0000860
Physical Location & Seq
Forward (+)
57920482 .. 57920952
471 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00264170.1

Sequence Viewer

Length: 471 bp
ATGGCTTCAAAGATGATTCAACTCAAATCAACAAACAAGACCAATAAGGTAGCTACTGCGAAAATGAGTAGAAACCTTAACAATCATACGATTCGGAGTGGGTCGTCTCAACTCGCGACATTCATAACTCATCCATCTCGGTCATGTCGGCCGATCATCACTTCGACAACAATGGGGGCAAACCTCACTAAGAAAAGATGGTCAATATGCTCAAGCGACTCAAGGGAACATTCTTCCTCGCACACCGCCGGTGTTGATCCGCGTACACACATACTCCCCAAATTGTCTCTTGATAACCAAACAGAAGAGCTTTTGGAAGACGCCTCTTTCGCTATGCAAGTCATGGTGATTGGAGTCACCACCGTGGAAGAACAAGTGACCCAAATAGCCCAATTGGCTGAAACGGTTGAGAAGCTACAGAGACTATTGAAGAGAAGGAAATGGAAATTGCCGCGCTCATGGAGAAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

156

Amino Acids

17.56

Weight (kDa)

10.43

Isoelectric Point (pI)

64.49

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 116, 262, 454
AciI CCGC 3 cut(s) 246, 260, 452
AclWI GGATC 1 cut(s) 251
AcoI YGGCCR 1 cut(s) 149
AcyI GRCGYC 1 cut(s) 321
AfaI GTAC 1 cut(s) 265
AgsI TTSAA 3 cut(s) 9, 20, 430
AleI CACNNNNGTG 1 cut(s) 362
AluBI AGCT 4 cut(s) 53, 310, 415, 468
AluI AGCT 4 cut(s) 53, 310, 415, 468
Alw26I GTCTC 3 cut(s) 111, 291, 415
AlwI GGATC 1 cut(s) 251
AoxI GGCC 1 cut(s) 149
ArsI GACNNNNNNTTYG 2 cut(s) 311, 343
AspLEI GCGC 1 cut(s) 456
AsuHPI GGTGA 2 cut(s) 349, 358
BbsI GAAGAC 1 cut(s) 324
BccI CCATC 2 cut(s) 142, 192
BcoDI GTCTC 3 cut(s) 111, 291, 415
BfaI CTAG 1 cut(s) 469
BfmI CTRYAG 1 cut(s) 416
BglI GCCNNNNNGGC 1 cut(s) 395
BisI GCNGC 1 cut(s) 452
BlsI GCNGC 1 cut(s) 453
BpiI GAAGAC 1 cut(s) 324
BpuEI CTTGAG 2 cut(s) 196, 205
BsaHI GRCGYC 1 cut(s) 321
BsaJI CCNNGG 1 cut(s) 363
BsaXI ACNNNNNCTCC 2 cut(s) 258, 288
Bse118I RCCGGY 1 cut(s) 248
BseDI CCNNGG 1 cut(s) 363
BseGI GGATG 1 cut(s) 130
BseX3I CGGCCG 1 cut(s) 149
Bsh1236I CGCG 3 cut(s) 116, 262, 454
Bsh1285I CGRYCG 1 cut(s) 152
BshFI GGCC 1 cut(s) 151
BsiEI CGRYCG 1 cut(s) 152
BsiSI CCGG 1 cut(s) 249
BsmAI GTCTC 3 cut(s) 111, 291, 415
BsmBI CGTCTC 1 cut(s) 111
BsnI GGCC 1 cut(s) 151
Bsp143I GATC 2 cut(s) 153, 256
Bsp68I TCGCGA 1 cut(s) 116
BspACI CCGC 3 cut(s) 246, 260, 452
BspANI GGCC 1 cut(s) 151
BspFNI CGCG 3 cut(s) 116, 262, 454
BspPI GGATC 1 cut(s) 251
BspQI GCTCTTC 1 cut(s) 300
BsrFI RCCGGY 1 cut(s) 248
BssAI RCCGGY 1 cut(s) 248
BssECI CCNNGG 1 cut(s) 363
BssMI GATC 2 cut(s) 153, 256
BssNI GRCGYC 1 cut(s) 321
Bst4CI ACNGT 2 cut(s) 364, 406
Bst6I CTCTTC 2 cut(s) 300, 425
BstACI GRCGYC 1 cut(s) 321
BstDEI CTNAG 1 cut(s) 189
BstDSI CCRYGG 1 cut(s) 363
BstF5I GGATG 1 cut(s) 130
BstFNI CGCG 3 cut(s) 116, 262, 454
BstHHI GCGC 1 cut(s) 456
BstKTI GATC 2 cut(s) 156, 259
BstMAI GTCTC 3 cut(s) 111, 291, 415
BstMBI GATC 2 cut(s) 153, 256
BstMCI CGRYCG 1 cut(s) 152
BstMWI GCNNNNNNNGC 2 cut(s) 329, 395
BstSFI CTRYAG 1 cut(s) 416
BstUI CGCG 3 cut(s) 116, 262, 454
BstV2I GAAGAC 1 cut(s) 324
BstZI CGGCCG 1 cut(s) 149
BsuRI GGCC 1 cut(s) 151
BtgI CCRYGG 1 cut(s) 363
BtsCI GGATG 1 cut(s) 130
BtuMI TCGCGA 1 cut(s) 116
CfoI GCGC 1 cut(s) 456
Cfr10I RCCGGY 1 cut(s) 248
CseI GACGC 1 cut(s) 329
Csp6I GTAC 1 cut(s) 264
CviAII CATG 3 cut(s) 144, 343, 459
CviJI RGCY 8 cut(s) 5, 53, 151, 310, 389, 398, 415, 468
CviKI_1 RGCY 8 cut(s) 5, 53, 151, 310, 389, 398, 415, 468
CviQI GTAC 1 cut(s) 264
DdeI CTNAG 1 cut(s) 189
DpnI GATC 2 cut(s) 155, 258
DpnII GATC 2 cut(s) 153, 256
EaeI YGGCCR 1 cut(s) 149
EagI CGGCCG 1 cut(s) 149
Eam1104I CTCTTC 2 cut(s) 300, 425
EarI CTCTTC 2 cut(s) 300, 425
EclXI CGGCCG 1 cut(s) 149
Eco52I CGGCCG 1 cut(s) 149
Esp3I CGTCTC 1 cut(s) 111
FaeI CATG 3 cut(s) 147, 346, 462
FaiI YATR 8 cut(s) 87, 125, 145, 208, 272, 335, 344, 460
FatI CATG 3 cut(s) 143, 342, 458
Fnu4HI GCNGC 1 cut(s) 452
FokI GGATG 1 cut(s) 117
Fsp4HI GCNGC 1 cut(s) 452
FspBI CTAG 1 cut(s) 469
GlaI GCGC 1 cut(s) 455
GluI GCNGC 1 cut(s) 452
HaeIII GGCC 1 cut(s) 151
HapII CCGG 1 cut(s) 249
HgaI GACGC 1 cut(s) 329
HhaI GCGC 1 cut(s) 456
Hin1I GRCGYC 1 cut(s) 321
Hin1II CATG 3 cut(s) 147, 346, 462
Hin6I GCGC 1 cut(s) 454
HinP1I GCGC 1 cut(s) 454
HinfI GANTC 4 cut(s) 16, 91, 218, 354
HpaII CCGG 1 cut(s) 249
HphI GGTGA 2 cut(s) 349, 358
Hpy166II GTNNAC 1 cut(s) 266
Hpy188I TCNGA 1 cut(s) 96
Hpy188III TCNNGA 2 cut(s) 115, 290
Hpy8I GTNNAC 1 cut(s) 266
HpyAV CCTTC 1 cut(s) 429
HpyCH4III ACNGT 2 cut(s) 364, 406
HpyCH4V TGCA 1 cut(s) 337
HpyF10VI GCNNNNNNNGC 2 cut(s) 329, 395
HpyF3I CTNAG 1 cut(s) 189
Hsp92I GRCGYC 1 cut(s) 321
Hsp92II CATG 3 cut(s) 147, 346, 462
HspAI GCGC 1 cut(s) 454
Kzo9I GATC 2 cut(s) 153, 256
LguI GCTCTTC 1 cut(s) 300
LpnPI CCDG 1 cut(s) 262
MaeI CTAG 1 cut(s) 469
MaeIII GTNAC 2 cut(s) 355, 376
MalI GATC 2 cut(s) 155, 258
MboI GATC 2 cut(s) 153, 256
MboII GAAGA 5 cut(s) 225, 317, 329, 380, 442
MfeI CAATTG 1 cut(s) 392
MluCI AATT 3 cut(s) 281, 392, 446
MlyI GAGTC 2 cut(s) 212, 363
MnlI CCTC 3 cut(s) 194, 247, 334
MseI TTAA 1 cut(s) 78
MslI CAYNNNNRTG 1 cut(s) 362
MspI CCGG 1 cut(s) 249
MunI CAATTG 1 cut(s) 392
MvnI CGCG 3 cut(s) 116, 262, 454
MwoI GCNNNNNNNGC 2 cut(s) 329, 395
NdeII GATC 2 cut(s) 153, 256
NlaIII CATG 3 cut(s) 147, 346, 462
NmuCI GTSAC 2 cut(s) 355, 376
NruI TCGCGA 1 cut(s) 116
OliI CACNNNNGTG 1 cut(s) 362
PciSI GCTCTTC 1 cut(s) 300
PfeI GAWTC 2 cut(s) 16, 91
PkrI GCNGC 1 cut(s) 453
PleI GAGTC 2 cut(s) 212, 362
PpsI GAGTC 2 cut(s) 212, 362
RruI TCGCGA 1 cut(s) 116
RsaI GTAC 1 cut(s) 265
RsaNI GTAC 1 cut(s) 264
RseI CAYNNNNRTG 1 cut(s) 362
SapI GCTCTTC 1 cut(s) 300
SaqAI TTAA 1 cut(s) 78
SatI GCNGC 1 cut(s) 452
Sau3AI GATC 2 cut(s) 153, 256
SchI GAGTC 2 cut(s) 212, 363
SetI ASST 7 cut(s) 51, 55, 78, 186, 312, 417, 470
SfcI CTRYAG 1 cut(s) 416
SgrAI CRCCGGYG 1 cut(s) 248
SmiMI CAYNNNNRTG 1 cut(s) 362
SmlI CTYRAG 2 cut(s) 211, 220
SmoI CTYRAG 2 cut(s) 211, 220
Sse9I AATT 3 cut(s) 281, 392, 446
SsiI CCGC 3 cut(s) 246, 260, 452
SspMI CTAG 1 cut(s) 469
TaaI ACNGT 2 cut(s) 364, 406
TaqI TCGA 1 cut(s) 164
TaqII GACCGA 1 cut(s) 129
TasI AATT 3 cut(s) 281, 392, 446
TauI GCSGC 1 cut(s) 454
TfiI GAWTC 2 cut(s) 16, 91
Tru1I TTAA 1 cut(s) 78
Tru9I TTAA 1 cut(s) 78
TseFI GTSAC 2 cut(s) 355, 376
Tsp45I GTSAC 2 cut(s) 355, 376
TspDTI ATGAA 1 cut(s) 112
XspI CTAG 1 cut(s) 469
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.