Rh1DG404200

Carboxylesterase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0000860
Physical Location & Seq
Reverse (-)
62352666 .. 62353328
663 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG404200.1

Sequence Viewer

Length: 663 bp
ATGTCAAATGAAAGGGCAGCAGCGGATTCCGATCCCAACTCCGGCGTTGATCCTTACCAGCGCCTCCAGATAGCACGCAATCCCGACGGCAGCATCACGCGCTTGTTCAAATGTCCAGACGCCCCGCCCTCATCTGATCCCAAGCTTCCCACTCCAGTTCTCACCAAAGACATCCCCATCAACCAATCAAATAATACCTGGCTTCGACTGTTCCTCCCCCGCCATGTGCTGGACCAAACCTCACCAACTACCAAGCTCCCTCTCGTCGTTTATTACCATGGTGGAGGCTTCATATTACTCAGCGCAGCCTCAACCATTTTCCATGATTTCTGTTTCAACTTTGCAGCCAGCGTCCCCGTGATCGTCGCATCTGTCGAGTACAGGTTGGCTCCTGAGCACCGGCTGCCGGCGGCTTATGATGATGCAGTGGAGGCGCTTCACTGGATCAAAACCTGCCAAGATGATTGGTTGAGAGACTACGCTGACCTCTCCAATTGTTTCCTTATGGGATCCAGTGCTGGGGGTAACATAGCCTACAATGCGGGACTTCGTGCGGCTGCTGAGGGCATTGACCTTCTCCACCCGTTGAAGATTAGAGGGCTGATATTGGTGGAAATTATTCTATGCACCGACGGTGTAAGTGACCTAACCCTTATAAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0009056 GO:0016787
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

220

Amino Acids

24.09

Weight (kDa)

5.42

Isoelectric Point (pI)

37.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BD-FAE PF20434 83 - 192 1.8e-13 BD-FAE
Abhydrolase_3 PF07859 89 - 203 3e-34 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 656
Acc36I ACCTGC 1 cut(s) 461
AccB7I CCANNNNNTGG 1 cut(s) 229
AccII CGCG 1 cut(s) 100
AciI CCGC 6 cut(s) 23, 125, 220, 410, 542, 554
AclWI GGATC 6 cut(s) 26, 44, 131, 452, 504, 517
AcyI GRCGYC 1 cut(s) 120
AfaI GTAC 1 cut(s) 380
AfiI CCNNNNNNNGG 4 cut(s) 41, 229, 406, 519
AgsI TTSAA 3 cut(s) 109, 337, 589
AjnI CCWGG 1 cut(s) 197
AluBI AGCT 2 cut(s) 145, 256
AluI AGCT 2 cut(s) 145, 256
Alw21I GWGCWC 1 cut(s) 399
Alw26I GTCTC 1 cut(s) 468
AlwI GGATC 6 cut(s) 26, 44, 131, 452, 504, 517
ApeKI GCWGC 7 cut(s) 17, 20, 90, 305, 344, 403, 557
Asp700I GAANNNNTTC 1 cut(s) 618
AspLEI GCGC 4 cut(s) 63, 102, 305, 436
AspS9I GGNCC 1 cut(s) 232
AsuHPI GGTGA 2 cut(s) 154, 234
AvaII GGWCC 1 cut(s) 232
BamHI GGATCC 1 cut(s) 509
Bbv12I GWGCWC 1 cut(s) 399
BbvCI CCTCAGC 1 cut(s) 561
BbvI GCAGC 7 cut(s) 29, 32, 102, 317, 356, 390, 544
BccI CCATC 1 cut(s) 185
BceAI ACGGC 1 cut(s) 103
BciT130I CCWGG 1 cut(s) 199
BcoDI GTCTC 1 cut(s) 468
BfoI RGCGCY 2 cut(s) 64, 437
BfuAI ACCTGC 1 cut(s) 461
BisI GCNGC 9 cut(s) 18, 21, 91, 306, 345, 404, 411, 555, 558
BlsI GCNGC 9 cut(s) 19, 22, 92, 307, 346, 405, 412, 556, 559
Bme1390I CCNGG 1 cut(s) 199
Bme18I GGWCC 1 cut(s) 232
BmgT120I GGNCC 1 cut(s) 232
BmiI GGNNCC 2 cut(s) 390, 511
BmrFI CCNGG 1 cut(s) 199
BmsI GCATC 3 cut(s) 102, 377, 412
BpmI CTGGAG 2 cut(s) 50, 138
Bpu10I CCTNAGC 2 cut(s) 393, 561
BsaBI GATNNNNATC 1 cut(s) 30
BsaHI GRCGYC 1 cut(s) 120
BsaJI CCNNGG 1 cut(s) 277
BsaXI ACNNNNNCTCC 2 cut(s) 198, 228
Bsc4I CCNNNNNNNGG 4 cut(s) 41, 229, 406, 519
Bse118I RCCGGY 2 cut(s) 399, 406
Bse1I ACTGG 3 cut(s) 155, 446, 513
Bse8I GATNNNNATC 1 cut(s) 30
BseBI CCWGG 1 cut(s) 199
BseDI CCNNGG 1 cut(s) 277
BseGI GGATG 1 cut(s) 171
BseJI GATNNNNATC 1 cut(s) 30
BseLI CCNNNNNNNGG 4 cut(s) 41, 229, 406, 519
BseMII CTCAG 3 cut(s) 313, 384, 552
BseNI ACTGG 3 cut(s) 155, 446, 513
BseXI GCAGC 7 cut(s) 29, 32, 102, 317, 356, 390, 544
BseYI CCCAGC 1 cut(s) 518
Bsh1236I CGCG 1 cut(s) 100
BsiHKAI GWGCWC 1 cut(s) 399
BsiSI CCGG 3 cut(s) 42, 400, 407
BslFI GGGAC 2 cut(s) 338, 558
BslI CCNNNNNNNGG 4 cut(s) 41, 229, 406, 519
BsmAI GTCTC 1 cut(s) 468
BsmFI GGGAC 2 cut(s) 338, 558
Bsp1286I GDGCHC 1 cut(s) 399
Bsp143I GATC 6 cut(s) 31, 49, 136, 360, 444, 509
Bsp19I CCATGG 1 cut(s) 277
BspACI CCGC 6 cut(s) 23, 125, 220, 410, 542, 554
BspCNI CTCAG 3 cut(s) 312, 385, 553
BspFNI CGCG 1 cut(s) 100
BspLI GGNNCC 2 cut(s) 390, 511
BspMI ACCTGC 1 cut(s) 461
BspPI GGATC 6 cut(s) 26, 44, 131, 452, 504, 517
BsrFI RCCGGY 2 cut(s) 399, 406
BsrI ACTGG 3 cut(s) 155, 446, 513
BssAI RCCGGY 2 cut(s) 399, 406
BssECI CCNNGG 1 cut(s) 277
BssMI GATC 6 cut(s) 31, 49, 136, 360, 444, 509
BssNI GRCGYC 1 cut(s) 120
BssT1I CCWWGG 1 cut(s) 277
Bst2UI CCWGG 1 cut(s) 199
Bst4CI ACNGT 2 cut(s) 210, 635
BstACI GRCGYC 1 cut(s) 120
BstAPI GCANNNNNTGC 1 cut(s) 403
BstC8I GCNNGC 3 cut(s) 76, 349, 408
BstDEI CTNAG 3 cut(s) 299, 393, 561
BstDSI CCRYGG 1 cut(s) 277
BstF5I GGATG 1 cut(s) 171
BstFNI CGCG 1 cut(s) 100
BstH2I RGCGCY 2 cut(s) 64, 437
BstHHI GCGC 4 cut(s) 63, 102, 305, 436
BstKTI GATC 6 cut(s) 34, 52, 139, 363, 447, 512
BstMAI GTCTC 1 cut(s) 468
BstMBI GATC 6 cut(s) 31, 49, 136, 360, 444, 509
BstMWI GCNNNNNNNGC 4 cut(s) 99, 403, 431, 539
BstNI CCWGG 1 cut(s) 199
BstSCI CCNGG 1 cut(s) 197
BstUI CGCG 1 cut(s) 100
BstV1I GCAGC 7 cut(s) 29, 32, 102, 317, 356, 390, 544
BstX2I RGATCY 1 cut(s) 509
BstYI RGATCY 1 cut(s) 509
BtgI CCRYGG 1 cut(s) 277
BtsCI GGATG 1 cut(s) 171
BtsI GCAGTG 1 cut(s) 432
BtsIMutI CAGTG 3 cut(s) 432, 439, 520
BveI ACCTGC 1 cut(s) 461
Cac8I GCNNGC 3 cut(s) 76, 349, 408
CfoI GCGC 4 cut(s) 63, 102, 305, 436
Cfr10I RCCGGY 2 cut(s) 399, 406
Cfr13I GGNCC 1 cut(s) 232
CseI GACGC 2 cut(s) 128, 340
Csp6I GTAC 1 cut(s) 379
CviAII CATG 3 cut(s) 224, 278, 323
CviQI GTAC 1 cut(s) 379
DdeI CTNAG 3 cut(s) 299, 393, 561
DpnI GATC 6 cut(s) 33, 51, 138, 362, 446, 511
DpnII GATC 6 cut(s) 31, 49, 136, 360, 444, 509
Eco130I CCWWGG 1 cut(s) 277
Eco47I GGWCC 1 cut(s) 232
EcoRII CCWGG 1 cut(s) 197
EcoT14I CCWWGG 1 cut(s) 277
ErhI CCWWGG 1 cut(s) 277
FaeI CATG 3 cut(s) 227, 281, 326
FaiI YATR 9 cut(s) 225, 279, 293, 324, 417, 506, 530, 625, 656
FaqI GGGAC 2 cut(s) 338, 558
FatI CATG 3 cut(s) 223, 277, 322
FauI CCCGC 3 cut(s) 132, 227, 535
Fnu4HI GCNGC 9 cut(s) 18, 21, 91, 306, 345, 404, 411, 555, 558
FokI GGATG 1 cut(s) 158
Fsp4HI GCNGC 9 cut(s) 18, 21, 91, 306, 345, 404, 411, 555, 558
GlaI GCGC 4 cut(s) 62, 101, 304, 435
GluI GCNGC 9 cut(s) 18, 21, 91, 306, 345, 404, 411, 555, 558
GsaI CCCAGC 1 cut(s) 522
GsuI CTGGAG 2 cut(s) 50, 138
HaeII RGCGCY 2 cut(s) 64, 437
HapII CCGG 3 cut(s) 42, 400, 407
HgaI GACGC 2 cut(s) 128, 340
HhaI GCGC 4 cut(s) 63, 102, 305, 436
Hin1I GRCGYC 1 cut(s) 120
Hin1II CATG 3 cut(s) 227, 281, 326
Hin6I GCGC 4 cut(s) 61, 100, 303, 434
HinP1I GCGC 4 cut(s) 61, 100, 303, 434
HindIII AAGCTT 1 cut(s) 143
HinfI GANTC 1 cut(s) 26
HpaII CCGG 3 cut(s) 42, 400, 407
HphI GGTGA 2 cut(s) 154, 234
Hpy188I TCNGA 2 cut(s) 31, 136
Hpy188III TCNNGA 4 cut(s) 67, 83, 116, 392
Hpy99I CGWCG 4 cut(s) 89, 269, 368, 635
HpyAV CCTTC 1 cut(s) 584
HpyCH4III ACNGT 2 cut(s) 210, 635
HpyCH4V TGCA 3 cut(s) 344, 425, 627
HpyF10VI GCNNNNNNNGC 4 cut(s) 99, 403, 431, 539
HpyF3I CTNAG 3 cut(s) 299, 393, 561
Hsp92I GRCGYC 1 cut(s) 120
Hsp92II CATG 3 cut(s) 227, 281, 326
HspAI GCGC 4 cut(s) 61, 100, 303, 434
KroI GCCGGC 1 cut(s) 406
KroNI GCCGGC 1 cut(s) 408
Kzo9I GATC 6 cut(s) 31, 49, 136, 360, 444, 509
LmnI GCTCC 2 cut(s) 261, 394
Lsp1109I GCAGC 7 cut(s) 29, 32, 102, 317, 356, 390, 544
LweI GCATC 3 cut(s) 102, 377, 412
MaeIII GTNAC 2 cut(s) 524, 641
MalI GATC 6 cut(s) 33, 51, 138, 362, 446, 511
MboI GATC 6 cut(s) 31, 49, 136, 360, 444, 509
MboII GAAGA 1 cut(s) 601
MfeI CAATTG 1 cut(s) 493
MflI RGATCY 1 cut(s) 509
MhlI GDGCHC 1 cut(s) 399
MluCI AATT 2 cut(s) 493, 615
MroNI GCCGGC 1 cut(s) 406
MroXI GAANNNNTTC 1 cut(s) 618
MspA1I CMGCKG 1 cut(s) 23
MspI CCGG 3 cut(s) 42, 400, 407
MspR9I CCNGG 1 cut(s) 199
MunI CAATTG 1 cut(s) 493
MvaI CCWGG 1 cut(s) 199
MvnI CGCG 1 cut(s) 100
MwoI GCNNNNNNNGC 4 cut(s) 99, 403, 431, 539
NaeI GCCGGC 1 cut(s) 408
NcoI CCATGG 1 cut(s) 277
NdeII GATC 6 cut(s) 31, 49, 136, 360, 444, 509
NgoMIV GCCGGC 1 cut(s) 406
NlaIII CATG 3 cut(s) 227, 281, 326
NlaIV GGNNCC 2 cut(s) 390, 511
NmuCI GTSAC 1 cut(s) 641
PcsI WCGNNNNNNNCGW 1 cut(s) 372
PdiI GCCGGC 1 cut(s) 408
PdmI GAANNNNTTC 1 cut(s) 618
PfeI GAWTC 1 cut(s) 26
PflMI CCANNNNNTGG 1 cut(s) 229
PkrI GCNGC 9 cut(s) 19, 22, 92, 307, 346, 405, 412, 556, 559
PsiI TTATAA 1 cut(s) 656
Psp6I CCWGG 1 cut(s) 197
PspFI CCCAGC 1 cut(s) 518
PspGI CCWGG 1 cut(s) 197
PspN4I GGNNCC 2 cut(s) 390, 511
PspPI GGNCC 1 cut(s) 232
PsuI RGATCY 1 cut(s) 509
RsaI GTAC 1 cut(s) 380
RsaNI GTAC 1 cut(s) 379
SatI GCNGC 9 cut(s) 18, 21, 91, 306, 345, 404, 411, 555, 558
Sau3AI GATC 6 cut(s) 31, 49, 136, 360, 444, 509
Sau96I GGNCC 1 cut(s) 232
ScrFI CCNGG 1 cut(s) 199
SduI GDGCHC 1 cut(s) 399
SetI ASST 9 cut(s) 147, 200, 242, 258, 386, 455, 489, 576, 648
SfaNI GCATC 3 cut(s) 102, 377, 412
SinI GGWCC 1 cut(s) 232
Sse9I AATT 2 cut(s) 493, 615
SsiI CCGC 6 cut(s) 23, 125, 220, 410, 542, 554
StyD4I CCNGG 1 cut(s) 197
StyI CCWWGG 1 cut(s) 277
TaaI ACNGT 2 cut(s) 210, 635
TaqI TCGA 2 cut(s) 205, 375
TasI AATT 2 cut(s) 493, 615
TatI WGTACW 1 cut(s) 378
TauI GCSGC 2 cut(s) 413, 557
TfiI GAWTC 1 cut(s) 26
TscAI CASTG 3 cut(s) 432, 446, 520
TseFI GTSAC 1 cut(s) 641
TseI GCWGC 7 cut(s) 17, 20, 90, 305, 344, 403, 557
Tsp45I GTSAC 1 cut(s) 641
TspDTI ATGAA 2 cut(s) 24, 280
TspRI CASTG 3 cut(s) 432, 446, 520
Van91I CCANNNNNTGG 1 cut(s) 229
VpaK11BI GGWCC 1 cut(s) 232
XmnI GAANNNNTTC 1 cut(s) 618
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.