RchiOBHm_Chr1g0376131

alpha/beta hydrolase fold

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0000860
Physical Location & Seq
Forward (+)
63404051 .. 63404989
939 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ59979

Sequence Viewer

Length: 939 bp
ATGTCAAAACCTGATCAATTCAAACCCAACATCATCTCCAATCCAGATGGAACCTACACGCGCCTCTTAAAATTTCCAAGCACCGAGGCCGACTCTACCTCAGCCACTAACCATGTACTCTCCAAAGACATCCCTGTAAACCCTGAAACCCAAACCATGGTCCGACTCTTCCTCCCTCGGAATGTCCTTGGCTCCAAAACCAAACTCCCTCTCATATTTTACTACCACGGTGGTGCGTTTCTCTATTTCAGCGCAACTACAACTGTTTTCCATGAGTTTTGTTCCAACATGGCTTTGGAACTCCAAGCTATTGTTGTCTCCTTGGACTACCGTCTTGCCCCTGAACACCGCCTCCCGGCAGCCTACGAGGATGCCATAGATGCGTTGCACTGGATCAAGACCACAGAGGAAGAGTGGGTAAGAGAATTTGCTGATTTCTCAAGCTGTTTTTTAATGGGTACTAGTTCTGGTGGAAACCTAGCTTACCATGCTGGGCTTCTTGCTTGTGAAGTTAGAGATCACCTTGGGCCTTTGGAAATCAAAGGACTGATATTGCACCATCCCTTTTTTGGTGGATCCGAAAGGACTGAGTCGGAACTGAGGATGGAGCATGATCGAATTTTGCCACTACGGGCAAGCGATGGAGCGTGGAAATTGGCGTTGCCTATTGGTGCTGACCGTGATCATGAGTATTGCAATCCAACAGTGGGCGATAGAGATGGTATTTGGGCTGCGATTCGGGCCATGGATTGTAGGGTCTTGGTTCTGGGTTGTTATGGAGATCCGATGATTGAGCGACAGATGGGGTTGGCGAAAATGGTAGAGGAGAAGGGTGTACGAACGGTGACTCATTTTGGCGATGGTTGTCATGGTTTGGAAATAACGGACGGGTCCAAAGCTCCGGGTTTATTTGTCGAATTGAAAAATTTCATATACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

312

Amino Acids

34.84

Weight (kDa)

5.61

Isoelectric Point (pI)

31.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BD-FAE PF20434 65 - 168 2e-13 BD-FAE
Abhydrolase_3 PF07859 72 - 290 3.6e-48 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 157
AccII CGCG 1 cut(s) 61
AciI CCGC 1 cut(s) 349
AclWI GGATC 4 cut(s) 401, 570, 583, 776
AcsI RAATTY 4 cut(s) 71, 425, 618, 925
AfaI GTAC 3 cut(s) 117, 460, 837
AfiI CCNNNNNNNGG 4 cut(s) 157, 355, 569, 707
AgsI TTSAA 2 cut(s) 22, 922
AhlI ACTAGT 1 cut(s) 461
AleI CACNNNNGTG 1 cut(s) 231
AloI GAACNNNNNNTCC 2 cut(s) 336, 368
AluBI AGCT 4 cut(s) 308, 444, 482, 899
AluI AGCT 4 cut(s) 308, 444, 482, 899
Alw26I GTCTC 1 cut(s) 322
AlwI GGATC 4 cut(s) 401, 570, 583, 776
AoxI GGCC 3 cut(s) 87, 527, 741
ApeKI GCWGC 2 cut(s) 359, 731
ApoI RAATTY 4 cut(s) 71, 425, 618, 925
Asp700I GAANNNNTTC 1 cut(s) 926
AspLEI GCGC 2 cut(s) 63, 254
AspS9I GGNCC 4 cut(s) 160, 527, 741, 891
AsuC2I CCSGG 2 cut(s) 356, 903
AsuHPI GGTGA 2 cut(s) 512, 856
AvaII GGWCC 2 cut(s) 160, 891
BamHI GGATCC 1 cut(s) 575
BbvCI CCTCAGC 1 cut(s) 100
BbvI GCAGC 2 cut(s) 371, 718
BccI CCATC 7 cut(s) 41, 567, 598, 635, 713, 796, 854
BclI TGATCA 2 cut(s) 13, 682
BcnI CCSGG 2 cut(s) 356, 903
BcoDI GTCTC 1 cut(s) 322
BcuI ACTAGT 1 cut(s) 461
BfaI CTAG 2 cut(s) 462, 479
BisI GCNGC 2 cut(s) 360, 732
BlsI GCNGC 2 cut(s) 361, 733
Bme1390I CCNGG 2 cut(s) 356, 903
Bme18I GGWCC 2 cut(s) 160, 891
BmgT120I GGNCC 4 cut(s) 160, 527, 741, 891
BmiI GGNNCC 4 cut(s) 52, 193, 577, 892
BmrFI CCNGG 2 cut(s) 356, 903
BmsI GCATC 2 cut(s) 361, 370
BoxI GACNNNNGTC 1 cut(s) 330
BplI GAGNNNNNCTC 2 cut(s) 77, 109
Bpu10I CCTNAGC 1 cut(s) 100
BpuEI CTTGAG 1 cut(s) 424
BpuMI CCSGG 2 cut(s) 356, 903
BsaJI CCNNGG 8 cut(s) 84, 156, 176, 187, 226, 321, 523, 744
BsaXI ACNNNNNCTCC 6 cut(s) 20, 50, 156, 186, 336, 366
Bsc4I CCNNNNNNNGG 4 cut(s) 157, 355, 569, 707
Bse1I ACTGG 1 cut(s) 395
BseDI CCNNGG 8 cut(s) 84, 156, 176, 187, 226, 321, 523, 744
BseGI GGATG 4 cut(s) 129, 376, 559, 609
BseLI CCNNNNNNNGG 4 cut(s) 157, 355, 569, 707
BseMII CTCAG 3 cut(s) 114, 579, 590
BseNI ACTGG 1 cut(s) 395
BseRI GAGGAG 1 cut(s) 839
BseXI GCAGC 2 cut(s) 371, 718
BseYI CCCAGC 1 cut(s) 491
Bsh1236I CGCG 1 cut(s) 61
BshFI GGCC 3 cut(s) 89, 529, 743
BsiSI CCGG 2 cut(s) 356, 902
BslI CCNNNNNNNGG 4 cut(s) 157, 355, 569, 707
BsmAI GTCTC 1 cut(s) 322
BsnI GGCC 3 cut(s) 89, 529, 743
Bsp143I GATC 7 cut(s) 13, 393, 517, 575, 613, 682, 781
Bsp19I CCATGG 2 cut(s) 156, 744
BspACI CCGC 1 cut(s) 349
BspANI GGCC 3 cut(s) 89, 529, 743
BspCNI CTCAG 3 cut(s) 113, 580, 591
BspFNI CGCG 1 cut(s) 61
BspHI TCATGA 1 cut(s) 685
BspLI GGNNCC 4 cut(s) 52, 193, 577, 892
BspPI GGATC 4 cut(s) 401, 570, 583, 776
BsrI ACTGG 1 cut(s) 395
BssECI CCNNGG 8 cut(s) 84, 156, 176, 187, 226, 321, 523, 744
BssMI GATC 7 cut(s) 13, 393, 517, 575, 613, 682, 781
BssT1I CCWWGG 5 cut(s) 156, 187, 321, 523, 744
Bst4CI ACNGT 6 cut(s) 230, 265, 332, 680, 706, 844
Bst6I CTCTTC 2 cut(s) 173, 405
BstC8I GCNNGC 1 cut(s) 637
BstDEI CTNAG 3 cut(s) 100, 588, 599
BstDSI CCRYGG 3 cut(s) 156, 226, 744
BstF5I GGATG 4 cut(s) 129, 376, 559, 609
BstFNI CGCG 1 cut(s) 61
BstHHI GCGC 2 cut(s) 63, 254
BstKTI GATC 7 cut(s) 16, 396, 520, 578, 616, 685, 784
BstMAI GTCTC 1 cut(s) 322
BstMBI GATC 7 cut(s) 13, 393, 517, 575, 613, 682, 781
BstMWI GCNNNNNNNGC 3 cut(s) 380, 488, 740
BstPAI GACNNNNGTC 1 cut(s) 330
BstSCI CCNGG 2 cut(s) 354, 901
BstUI CGCG 1 cut(s) 61
BstV1I GCAGC 2 cut(s) 371, 718
BstX2I RGATCY 2 cut(s) 575, 781
BstYI RGATCY 2 cut(s) 575, 781
BsuRI GGCC 3 cut(s) 89, 529, 743
BtgI CCRYGG 3 cut(s) 156, 226, 744
BtgZI GCGATG 2 cut(s) 654, 873
BtsCI GGATG 4 cut(s) 129, 376, 559, 609
BtsIMutI CAGTG 2 cut(s) 388, 711
Cac8I GCNNGC 1 cut(s) 637
CciI TCATGA 1 cut(s) 685
CfoI GCGC 2 cut(s) 63, 254
Cfr13I GGNCC 4 cut(s) 160, 527, 741, 891
Csp6I GTAC 3 cut(s) 116, 459, 836
CviAII CATG 9 cut(s) 113, 157, 272, 289, 488, 611, 686, 745, 869
CviQI GTAC 3 cut(s) 116, 459, 836
DdeI CTNAG 3 cut(s) 100, 588, 599
DpnI GATC 7 cut(s) 15, 395, 519, 577, 615, 684, 783
DpnII GATC 7 cut(s) 13, 393, 517, 575, 613, 682, 781
Eam1104I CTCTTC 2 cut(s) 173, 405
EarI CTCTTC 2 cut(s) 173, 405
Eco130I CCWWGG 5 cut(s) 156, 187, 321, 523, 744
Eco47I GGWCC 2 cut(s) 160, 891
EcoT14I CCWWGG 5 cut(s) 156, 187, 321, 523, 744
ErhI CCWWGG 5 cut(s) 156, 187, 321, 523, 744
FaeI CATG 9 cut(s) 116, 160, 275, 292, 491, 614, 689, 748, 872
FatI CATG 9 cut(s) 112, 156, 271, 288, 487, 610, 685, 744, 868
FbaI TGATCA 2 cut(s) 13, 682
Fnu4HI GCNGC 2 cut(s) 360, 732
FokI GGATG 4 cut(s) 116, 383, 546, 616
Fsp4HI GCNGC 2 cut(s) 360, 732
FspBI CTAG 2 cut(s) 462, 479
GlaI GCGC 2 cut(s) 62, 253
GluI GCNGC 2 cut(s) 360, 732
GsaI CCCAGC 1 cut(s) 495
HaeIII GGCC 3 cut(s) 89, 529, 743
HapII CCGG 2 cut(s) 356, 902
HhaI GCGC 2 cut(s) 63, 254
Hin1II CATG 9 cut(s) 116, 160, 275, 292, 491, 614, 689, 748, 872
Hin6I GCGC 2 cut(s) 61, 252
HinP1I GCGC 2 cut(s) 61, 252
HinfI GANTC 5 cut(s) 92, 165, 590, 736, 847
HpaII CCGG 2 cut(s) 356, 902
HphI GGTGA 2 cut(s) 512, 856
Hpy166II GTNNAC 2 cut(s) 139, 836
Hpy188I TCNGA 5 cut(s) 164, 180, 580, 595, 786
Hpy188III TCNNGA 3 cut(s) 44, 397, 686
Hpy8I GTNNAC 2 cut(s) 139, 836
HpyAV CCTTC 1 cut(s) 823
HpyCH4III ACNGT 6 cut(s) 230, 265, 332, 680, 706, 844
HpyCH4V TGCA 3 cut(s) 388, 556, 696
HpyF10VI GCNNNNNNNGC 3 cut(s) 380, 488, 740
HpyF3I CTNAG 3 cut(s) 100, 588, 599
Hsp92II CATG 9 cut(s) 116, 160, 275, 292, 491, 614, 689, 748, 872
HspAI GCGC 2 cut(s) 61, 252
Ksp22I TGATCA 2 cut(s) 13, 682
Kzo9I GATC 7 cut(s) 13, 393, 517, 575, 613, 682, 781
LmnI GCTCC 4 cut(s) 197, 607, 644, 904
Lsp1109I GCAGC 2 cut(s) 371, 718
LweI GCATC 2 cut(s) 361, 370
MaeI CTAG 2 cut(s) 462, 479
MaeIII GTNAC 1 cut(s) 844
MalI GATC 7 cut(s) 15, 395, 519, 577, 615, 684, 783
MboI GATC 7 cut(s) 13, 393, 517, 575, 613, 682, 781
MboII GAAGA 2 cut(s) 160, 422
MflI RGATCY 2 cut(s) 575, 781
MluCI AATT 7 cut(s) 17, 71, 425, 618, 653, 917, 925
MlyI GAGTC 4 cut(s) 86, 159, 599, 841
MmeI TCCRAC 4 cut(s) 187, 309, 573, 725
MroXI GAANNNNTTC 1 cut(s) 926
MseI TTAA 2 cut(s) 68, 452
MslI CAYNNNNRTG 1 cut(s) 231
MspI CCGG 2 cut(s) 356, 902
MspR9I CCNGG 2 cut(s) 356, 903
MvnI CGCG 1 cut(s) 61
MwoI GCNNNNNNNGC 3 cut(s) 380, 488, 740
NciI CCSGG 2 cut(s) 356, 903
NcoI CCATGG 2 cut(s) 156, 744
NdeII GATC 7 cut(s) 13, 393, 517, 575, 613, 682, 781
NlaIII CATG 9 cut(s) 116, 160, 275, 292, 491, 614, 689, 748, 872
NlaIV GGNNCC 4 cut(s) 52, 193, 577, 892
NmuCI GTSAC 1 cut(s) 844
OliI CACNNNNGTG 1 cut(s) 231
PagI TCATGA 1 cut(s) 685
PdmI GAANNNNTTC 1 cut(s) 926
PfeI GAWTC 1 cut(s) 736
PflFI GACNNNGTC 1 cut(s) 589
PflMI CCANNNNNTGG 1 cut(s) 157
PkrI GCNGC 2 cut(s) 361, 733
PleI GAGTC 4 cut(s) 86, 159, 598, 841
PpsI GAGTC 4 cut(s) 86, 159, 598, 841
PshAI GACNNNNGTC 1 cut(s) 330
PspFI CCCAGC 1 cut(s) 491
PspN4I GGNNCC 4 cut(s) 52, 193, 577, 892
PspPI GGNCC 4 cut(s) 160, 527, 741, 891
PsuI RGATCY 2 cut(s) 575, 781
PsyI GACNNNGTC 1 cut(s) 589
RsaI GTAC 3 cut(s) 117, 460, 837
RsaNI GTAC 3 cut(s) 116, 459, 836
RseI CAYNNNNRTG 1 cut(s) 231
SaqAI TTAA 2 cut(s) 68, 452
SatI GCNGC 2 cut(s) 360, 732
Sau3AI GATC 7 cut(s) 13, 393, 517, 575, 613, 682, 781
Sau96I GGNCC 4 cut(s) 160, 527, 741, 891
SchI GAGTC 4 cut(s) 86, 159, 599, 841
ScrFI CCNGG 2 cut(s) 356, 903
SetI ASST 9 cut(s) 13, 56, 101, 310, 446, 480, 484, 525, 901
SfaNI GCATC 2 cut(s) 361, 370
SinI GGWCC 2 cut(s) 160, 891
SmiMI CAYNNNNRTG 1 cut(s) 231
SmlI CTYRAG 1 cut(s) 439
SmoI CTYRAG 1 cut(s) 439
SpeI ACTAGT 1 cut(s) 461
Sse9I AATT 7 cut(s) 17, 71, 425, 618, 653, 917, 925
SsiI CCGC 1 cut(s) 349
SspMI CTAG 2 cut(s) 462, 479
StyD4I CCNGG 2 cut(s) 354, 901
StyI CCWWGG 5 cut(s) 156, 187, 321, 523, 744
TaaI ACNGT 6 cut(s) 230, 265, 332, 680, 706, 844
TaqI TCGA 2 cut(s) 616, 915
TasI AATT 7 cut(s) 17, 71, 425, 618, 653, 917, 925
TatI WGTACW 1 cut(s) 115
TfiI GAWTC 1 cut(s) 736
Tru1I TTAA 2 cut(s) 68, 452
Tru9I TTAA 2 cut(s) 68, 452
TscAI CASTG 2 cut(s) 395, 711
TseFI GTSAC 1 cut(s) 844
TseI GCWGC 2 cut(s) 359, 731
Tsp45I GTSAC 1 cut(s) 844
TspDTI ATGAA 1 cut(s) 919
TspGWI ACGGA 1 cut(s) 899
TspRI CASTG 2 cut(s) 395, 711
Tth111I GACNNNGTC 1 cut(s) 589
Van91I CCANNNNNTGG 1 cut(s) 157
VpaK11BI GGWCC 2 cut(s) 160, 891
XapI RAATTY 4 cut(s) 71, 425, 618, 925
XcmI CCANNNNNNNNNTGG 2 cut(s) 292, 566
XmnI GAANNNNTTC 1 cut(s) 926
XspI CTAG 2 cut(s) 462, 479
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.