FvH4_7g28650

alpha/beta hydrolase fold

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0000860
Physical Location & Seq
Forward (+)
21207334 .. 21208477
1144 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g28650.t1

Sequence Viewer

Length: 939 bp
ATGTCAAACACTGATCAATTCAAACCCAAAATAATCTCTAATCCAGATGGAACATACACGCGCCTCATAAAATTTCCAAGCACTGATGCCGACTCTACCTCCGCCACTAACCCAGTACTCTCCAAAGACATCCCTGTAAACCCTAAGACCCAAACCATGGTCCGATTCTTCCTCCCCCGGAATGTCCTTGGCTCAAAAACCAAACTCCCTCTCATATTTTACTACCACGGCGGTGCGTTTCTCTATTGCAGCGCAACTACAACGGTTTTCCATGAGTTCTGTTCCAACATGGCTTCGGAACTCCAAGCTATTGTAGTATCCGTTGACTACCGTCTCGCCCCCGAACACCGCCTCCCTGCAGCCTACGAGGACGCCGTAGATGTGTTGTACTGGATCAAAACCGCAGAGGAAGTATGGGTAAGAGAGTTTGCTGACTTCTCAAGCTGTTTTCTAATGGGTACTAGTTCTGGTGGAAACCTAGCTTACCATGCAGGGCTTCTTGCTGGTGAAGTTAGTGACCAACTTAGGCACTTGAAAATCAAAGGACTCATACTGCACCATCCCTTCTTTGGTGGATCCGAGAGGACCGAATCGGAGCTGAAGATGGAGCATGATCGGGTTTTGCCACTCCGGGCAAGCGATGCGGCCTGGAAATTGGTGTTGCCTATAGGTGCTGACCGTGATCATGAGTATTGCAACCCAATGGTGGTTGACAGAAATGATGCTTGGGGTGCGATGAGGGCCGTGGATTGTAGGGTTTTGGTTTTGGGTTGTTATGGAGATCCGATGATTGACCGGCAGATGGGGTTGGCGAGAATGGTAGAGGAGAAGGGTGTGCAGACGGCGACCCACTTTGGCGATGGTTGTCATGGTTTGGAAATAACTGACCCGTCCAAAGCTCCGGGTTTATTTGCCGTGTTGAAAAATTTCATATACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

313

Amino Acids

34.73

Weight (kDa)

5.99

Isoelectric Point (pI)

23.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BD-FAE PF20434 65 - 169 1.5e-12 BD-FAE
Abhydrolase_3 PF07859 72 - 288 9.2e-51 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 157
AccII CGCG 1 cut(s) 61
AciI CCGC 5 cut(s) 102, 231, 349, 402, 644
AclWI GGATC 4 cut(s) 401, 570, 583, 776
AcsI RAATTY 2 cut(s) 71, 925
AcuI CTGAAG 1 cut(s) 620
AcyI GRCGYC 1 cut(s) 372
AfaI GTAC 3 cut(s) 117, 389, 460
AfiI CCNNNNNNNGG 4 cut(s) 157, 569, 706, 802
AgsI TTSAA 3 cut(s) 22, 535, 922
AhlI ACTAGT 1 cut(s) 461
AjnI CCWGG 1 cut(s) 647
AjuI GAANNNNNNNTTGG 2 cut(s) 709, 741
AleI CACNNNNGTG 1 cut(s) 231
AloI GAACNNNNNNTCC 2 cut(s) 336, 368
AluBI AGCT 5 cut(s) 308, 444, 482, 598, 899
AluI AGCT 5 cut(s) 308, 444, 482, 598, 899
Alw26I GTCTC 1 cut(s) 338
AlwI GGATC 4 cut(s) 401, 570, 583, 776
AoxI GGCC 2 cut(s) 645, 741
ApeKI GCWGC 2 cut(s) 249, 359
ApoI RAATTY 2 cut(s) 71, 925
Asp700I GAANNNNTTC 1 cut(s) 926
AspLEI GCGC 2 cut(s) 63, 254
AspS9I GGNCC 3 cut(s) 160, 585, 741
AsuC2I CCSGG 3 cut(s) 178, 632, 903
AsuHPI GGTGA 1 cut(s) 518
AvaII GGWCC 2 cut(s) 160, 585
BamHI GGATCC 1 cut(s) 575
BbvI GCAGC 2 cut(s) 261, 371
BccI CCATC 5 cut(s) 41, 567, 598, 796, 854
BceAI ACGGC 5 cut(s) 244, 359, 728, 858, 899
BciT130I CCWGG 1 cut(s) 649
BciVI GTATCC 1 cut(s) 328
BclI TGATCA 2 cut(s) 13, 682
BcnI CCSGG 3 cut(s) 178, 632, 903
BcoDI GTCTC 1 cut(s) 338
BcuI ACTAGT 1 cut(s) 461
BfaI CTAG 3 cut(s) 462, 479, 937
BfmI CTRYAG 2 cut(s) 357, 666
BfuI GTATCC 1 cut(s) 328
BisI GCNGC 3 cut(s) 250, 360, 645
BlsI GCNGC 3 cut(s) 251, 361, 646
BmcAI AGTACT 1 cut(s) 117
Bme1390I CCNGG 4 cut(s) 178, 632, 649, 903
Bme18I GGWCC 2 cut(s) 160, 585
BmgT120I GGNCC 3 cut(s) 160, 585, 741
BmiI GGNNCC 1 cut(s) 577
BmrFI CCNGG 4 cut(s) 178, 632, 649, 903
BmrI ACTGGG 1 cut(s) 107
BmsI GCATC 3 cut(s) 76, 631, 712
BmuI ACTGGG 1 cut(s) 107
BoxI GACNNNNGTC 1 cut(s) 330
BpuEI CTTGAG 1 cut(s) 424
BpuMI CCSGG 3 cut(s) 178, 632, 903
BsaHI GRCGYC 1 cut(s) 372
BsaJI CCNNGG 5 cut(s) 156, 176, 187, 226, 744
BsaXI ACNNNNNCTCC 4 cut(s) 83, 113, 336, 366
Bsc4I CCNNNNNNNGG 4 cut(s) 157, 569, 706, 802
Bse118I RCCGGY 1 cut(s) 795
Bse1I ACTGG 2 cut(s) 113, 395
BseBI CCWGG 1 cut(s) 649
BseDI CCNNGG 5 cut(s) 156, 176, 187, 226, 744
BseGI GGATG 2 cut(s) 129, 559
BseLI CCNNNNNNNGG 4 cut(s) 157, 569, 706, 802
BseNI ACTGG 2 cut(s) 113, 395
BseRI GAGGAG 1 cut(s) 839
BseXI GCAGC 2 cut(s) 261, 371
BsgI GTGCAG 2 cut(s) 539, 857
Bsh1236I CGCG 1 cut(s) 61
BshFI GGCC 2 cut(s) 647, 743
BsiSI CCGG 4 cut(s) 178, 631, 796, 902
BslI CCNNNNNNNGG 4 cut(s) 157, 569, 706, 802
BsmAI GTCTC 1 cut(s) 338
BsmBI CGTCTC 1 cut(s) 338
BsnI GGCC 2 cut(s) 647, 743
Bsp143I GATC 6 cut(s) 13, 393, 575, 613, 682, 781
Bsp19I CCATGG 1 cut(s) 156
BspACI CCGC 5 cut(s) 102, 231, 349, 402, 644
BspANI GGCC 2 cut(s) 647, 743
BspFNI CGCG 1 cut(s) 61
BspHI TCATGA 1 cut(s) 685
BspLI GGNNCC 1 cut(s) 577
BspMAI CTGCAG 1 cut(s) 361
BspPI GGATC 4 cut(s) 401, 570, 583, 776
BsrFI RCCGGY 1 cut(s) 795
BsrI ACTGG 2 cut(s) 113, 395
BssAI RCCGGY 1 cut(s) 795
BssECI CCNNGG 5 cut(s) 156, 176, 187, 226, 744
BssMI GATC 6 cut(s) 13, 393, 575, 613, 682, 781
BssNI GRCGYC 1 cut(s) 372
BssT1I CCWWGG 2 cut(s) 156, 187
Bst2UI CCWGG 1 cut(s) 649
Bst4CI ACNGT 3 cut(s) 265, 332, 680
BstACI GRCGYC 1 cut(s) 372
BstAPI GCANNNNNTGC 1 cut(s) 641
BstC8I GCNNGC 1 cut(s) 637
BstDEI CTNAG 2 cut(s) 144, 524
BstDSI CCRYGG 3 cut(s) 156, 226, 744
BstF5I GGATG 2 cut(s) 129, 559
BstFNI CGCG 1 cut(s) 61
BstHHI GCGC 2 cut(s) 63, 254
BstKTI GATC 6 cut(s) 16, 396, 578, 616, 685, 784
BstMAI GTCTC 1 cut(s) 338
BstMBI GATC 6 cut(s) 13, 393, 575, 613, 682, 781
BstMWI GCNNNNNNNGC 4 cut(s) 488, 641, 731, 740
BstNI CCWGG 1 cut(s) 649
BstPAI GACNNNNGTC 1 cut(s) 330
BstSCI CCNGG 4 cut(s) 176, 630, 647, 901
BstSFI CTRYAG 2 cut(s) 357, 666
BstUI CGCG 1 cut(s) 61
BstV1I GCAGC 2 cut(s) 261, 371
BstX2I RGATCY 2 cut(s) 575, 781
BstYI RGATCY 2 cut(s) 575, 781
BsuI GTATCC 1 cut(s) 328
BsuRI GGCC 2 cut(s) 647, 743
BtgI CCRYGG 3 cut(s) 156, 226, 744
BtgZI GCGATG 3 cut(s) 654, 749, 873
BtsCI GGATG 2 cut(s) 129, 559
BtsIMutI CAGTG 2 cut(s) 9, 81
Cac8I GCNNGC 1 cut(s) 637
CciI TCATGA 1 cut(s) 685
CfoI GCGC 2 cut(s) 63, 254
Cfr10I RCCGGY 1 cut(s) 795
Cfr13I GGNCC 3 cut(s) 160, 585, 741
CseI GACGC 1 cut(s) 380
Csp6I GTAC 3 cut(s) 116, 388, 459
CviAII CATG 7 cut(s) 157, 272, 289, 488, 611, 686, 869
CviQI GTAC 3 cut(s) 116, 388, 459
DdeI CTNAG 2 cut(s) 144, 524
DpnI GATC 6 cut(s) 15, 395, 577, 615, 684, 783
DpnII GATC 6 cut(s) 13, 393, 575, 613, 682, 781
EciI GGCGGA 1 cut(s) 91
Eco130I CCWWGG 2 cut(s) 156, 187
Eco47I GGWCC 2 cut(s) 160, 585
Eco57I CTGAAG 1 cut(s) 620
EcoRII CCWGG 1 cut(s) 647
EcoT14I CCWWGG 2 cut(s) 156, 187
ErhI CCWWGG 2 cut(s) 156, 187
Esp3I CGTCTC 1 cut(s) 338
FaeI CATG 7 cut(s) 160, 275, 292, 491, 614, 689, 872
FatI CATG 7 cut(s) 156, 271, 288, 487, 610, 685, 868
FbaI TGATCA 2 cut(s) 13, 682
Fnu4HI GCNGC 3 cut(s) 250, 360, 645
FokI GGATG 2 cut(s) 116, 546
Fsp4HI GCNGC 3 cut(s) 250, 360, 645
FspBI CTAG 3 cut(s) 462, 479, 937
GlaI GCGC 2 cut(s) 62, 253
GluI GCNGC 3 cut(s) 250, 360, 645
HaeIII GGCC 2 cut(s) 647, 743
HapII CCGG 4 cut(s) 178, 631, 796, 902
HgaI GACGC 1 cut(s) 380
HhaI GCGC 2 cut(s) 63, 254
Hin1I GRCGYC 1 cut(s) 372
Hin1II CATG 7 cut(s) 160, 275, 292, 491, 614, 689, 872
Hin6I GCGC 2 cut(s) 61, 252
HinP1I GCGC 2 cut(s) 61, 252
HincII GTYRAC 2 cut(s) 325, 712
HindII GTYRAC 2 cut(s) 325, 712
HinfI GANTC 4 cut(s) 92, 165, 546, 590
HpaII CCGG 4 cut(s) 178, 631, 796, 902
HphI GGTGA 1 cut(s) 518
Hpy166II GTNNAC 3 cut(s) 139, 325, 712
Hpy188I TCNGA 5 cut(s) 164, 298, 580, 595, 786
Hpy188III TCNNGA 2 cut(s) 44, 686
Hpy8I GTNNAC 3 cut(s) 139, 325, 712
HpyAV CCTTC 2 cut(s) 574, 823
HpyCH4III ACNGT 3 cut(s) 265, 332, 680
HpyCH4V TGCA 6 cut(s) 249, 359, 491, 556, 696, 838
HpyF10VI GCNNNNNNNGC 4 cut(s) 488, 641, 731, 740
HpyF3I CTNAG 2 cut(s) 144, 524
Hsp92I GRCGYC 1 cut(s) 372
Hsp92II CATG 7 cut(s) 160, 275, 292, 491, 614, 689, 872
HspAI GCGC 2 cut(s) 61, 252
Ksp22I TGATCA 2 cut(s) 13, 682
Kzo9I GATC 6 cut(s) 13, 393, 575, 613, 682, 781
LmnI GCTCC 3 cut(s) 595, 607, 904
Lsp1109I GCAGC 2 cut(s) 261, 371
LweI GCATC 3 cut(s) 76, 631, 712
MaeI CTAG 3 cut(s) 462, 479, 937
MaeIII GTNAC 1 cut(s) 515
MalI GATC 6 cut(s) 15, 395, 577, 615, 684, 783
MboI GATC 6 cut(s) 13, 393, 575, 613, 682, 781
MboII GAAGA 2 cut(s) 160, 613
MflI RGATCY 2 cut(s) 575, 781
MluCI AATT 4 cut(s) 17, 71, 653, 925
MlyI GAGTC 2 cut(s) 86, 540
MmeI TCCRAC 1 cut(s) 309
MroXI GAANNNNTTC 1 cut(s) 926
MslI CAYNNNNRTG 1 cut(s) 231
MspI CCGG 4 cut(s) 178, 631, 796, 902
MspR9I CCNGG 4 cut(s) 178, 632, 649, 903
MvaI CCWGG 1 cut(s) 649
MvnI CGCG 1 cut(s) 61
MwoI GCNNNNNNNGC 4 cut(s) 488, 641, 731, 740
NciI CCSGG 3 cut(s) 178, 632, 903
NcoI CCATGG 1 cut(s) 156
NdeII GATC 6 cut(s) 13, 393, 575, 613, 682, 781
NlaIII CATG 7 cut(s) 160, 275, 292, 491, 614, 689, 872
NlaIV GGNNCC 1 cut(s) 577
NmuCI GTSAC 1 cut(s) 515
OliI CACNNNNGTG 1 cut(s) 231
PagI TCATGA 1 cut(s) 685
PcsI WCGNNNNNNNCGW 1 cut(s) 372
PdmI GAANNNNTTC 1 cut(s) 926
PfeI GAWTC 2 cut(s) 165, 590
PflMI CCANNNNNTGG 1 cut(s) 157
PkrI GCNGC 3 cut(s) 251, 361, 646
PleI GAGTC 2 cut(s) 86, 540
PpsI GAGTC 2 cut(s) 86, 540
PshAI GACNNNNGTC 1 cut(s) 330
Psp6I CCWGG 1 cut(s) 647
PspGI CCWGG 1 cut(s) 647
PspN4I GGNNCC 1 cut(s) 577
PspPI GGNCC 3 cut(s) 160, 585, 741
PstI CTGCAG 1 cut(s) 361
PsuI RGATCY 2 cut(s) 575, 781
RsaI GTAC 3 cut(s) 117, 389, 460
RsaNI GTAC 3 cut(s) 116, 388, 459
RseI CAYNNNNRTG 1 cut(s) 231
SatI GCNGC 3 cut(s) 250, 360, 645
Sau3AI GATC 6 cut(s) 13, 393, 575, 613, 682, 781
Sau96I GGNCC 3 cut(s) 160, 585, 741
ScaI AGTACT 1 cut(s) 117
SchI GAGTC 2 cut(s) 86, 540
ScrFI CCNGG 4 cut(s) 178, 632, 649, 903
SetI ASST 8 cut(s) 101, 310, 446, 480, 484, 600, 673, 901
SfaNI GCATC 3 cut(s) 76, 631, 712
SfcI CTRYAG 2 cut(s) 357, 666
SinI GGWCC 2 cut(s) 160, 585
SmiMI CAYNNNNRTG 1 cut(s) 231
SmlI CTYRAG 1 cut(s) 439
SmoI CTYRAG 1 cut(s) 439
SpeI ACTAGT 1 cut(s) 461
Sse9I AATT 4 cut(s) 17, 71, 653, 925
SsiI CCGC 5 cut(s) 102, 231, 349, 402, 644
SspMI CTAG 3 cut(s) 462, 479, 937
StyD4I CCNGG 4 cut(s) 176, 630, 647, 901
StyI CCWWGG 2 cut(s) 156, 187
TaaI ACNGT 3 cut(s) 265, 332, 680
TaqII GACCGA 1 cut(s) 602
TasI AATT 4 cut(s) 17, 71, 653, 925
TatI WGTACW 2 cut(s) 115, 387
TauI GCSGC 1 cut(s) 647
TfiI GAWTC 2 cut(s) 165, 590
TscAI CASTG 2 cut(s) 16, 88
TseFI GTSAC 1 cut(s) 515
TseI GCWGC 2 cut(s) 249, 359
Tsp45I GTSAC 1 cut(s) 515
TspDTI ATGAA 1 cut(s) 919
TspGWI ACGGA 1 cut(s) 310
TspRI CASTG 2 cut(s) 16, 88
Van91I CCANNNNNTGG 1 cut(s) 157
VpaK11BI GGWCC 2 cut(s) 160, 585
XapI RAATTY 2 cut(s) 71, 925
XcmI CCANNNNNNNNNTGG 2 cut(s) 566, 857
XmnI GAANNNNTTC 1 cut(s) 926
XspI CTAG 3 cut(s) 462, 479, 937
ZrmI AGTACT 1 cut(s) 117
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.