Rmu_sc0005594.1_g000007

Carboxylesterase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005594.1
OG0000860
Physical Location & Seq
Forward (+)
40827 .. 41825
999 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0005594.1_g000007.1.cds

Sequence Viewer

Length: 999 bp
atgtcaaatgaaagggcagcagcggattccgatcccaactccggcgttgatccttaccagcgcctccagatagcacgcaatcccgacggcagcatcacgcgcttgttcaaatgtccagacgccccgccctcatcggatcccaagcttcccactccagttctcaccaaagacatccccataaaccaatcaaataatacctggcttcgactgttcctcccccgccatgtactggaccaaacctcaccaactaccaagctccctctcgtcgtttattaccatggtggaggcttcatattacttagcgcagcctcaaccattttccatgatttctgtttcaactttgcagccggcgtccccgtgatcgtcgcatctgtcgagtacaggttggctcctgagcaccggctgccggcggcttatgatgatgcagtggaggcgcttcactggatcaaaacctgccaagatgattggttgagagactacgctgacctctccacttgtttccttatgggatccagtgctgggggtaacatagcctacaatgcgggacttcgtgctgctgctgagggcattgaccttctccacccgttgaagattagagggctgatattggtgcaaccgtattttggggggaatcgaaggactagttcagagttgaggttggtgaacaaccctgttttggcgccaagtgtctgtgatctcatgtgggacttgtcgttgcccatcggggctgaccgtgatcacgagtattgcaatccaatggcgggtggcgattctaatggctttgatcaagtgaaagcacttgggtggaaagttttggtgagtggctctgatggagacccactgatcgatcgtcagattgagctagtgaagatgttggagggaaagggtgtacatgtggtgagtcagtttattgaaggaggttatcatggggctcaagatgttgaaccctccaaagccagggatgtgtttgtgattgttaagcattttatatttccttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0009056 GO:0016787
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

332

Amino Acids

36.38

Weight (kDa)

5.54

Isoelectric Point (pI)

38.94

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 461
AccB1I GGYRCC 1 cut(s) 679
AccB7I CCANNNNNTGG 1 cut(s) 229
AccII CGCG 1 cut(s) 100
AciI CCGC 6 cut(s) 23, 125, 220, 410, 542, 761
AclWI GGATC 7 cut(s) 26, 44, 131, 144, 452, 504, 517
AcyI GRCGYC 3 cut(s) 120, 351, 680
AfaI GTAC 3 cut(s) 228, 380, 891
AfiI CCNNNNNNNGG 8 cut(s) 41, 229, 406, 519, 623, 676, 761, 957
AflIII ACRYGT 1 cut(s) 892
AgsI TTSAA 5 cut(s) 109, 337, 589, 914, 944
AhlI ACTAGT 1 cut(s) 641
AjnI CCWGG 2 cut(s) 197, 956
AleI CACNNNNGTG 1 cut(s) 802
AluBI AGCT 3 cut(s) 145, 256, 862
AluI AGCT 3 cut(s) 145, 256, 862
Alw21I GWGCWC 1 cut(s) 399
Alw26I GTCTC 2 cut(s) 468, 828
AlwI GGATC 7 cut(s) 26, 44, 131, 144, 452, 504, 517
ApeKI GCWGC 8 cut(s) 17, 20, 90, 305, 344, 403, 554, 557
AspLEI GCGC 5 cut(s) 63, 102, 305, 436, 682
AspS9I GGNCC 1 cut(s) 232
AsuHPI GGTGA 5 cut(s) 154, 234, 673, 829, 910
AvaII GGWCC 1 cut(s) 232
BamHI GGATCC 2 cut(s) 136, 509
BanI GGYRCC 1 cut(s) 679
BanII GRGCYC 1 cut(s) 934
BauI CACGAG 1 cut(s) 740
Bbv12I GWGCWC 1 cut(s) 399
BbvCI CCTCAGC 1 cut(s) 561
BbvI GCAGC 8 cut(s) 29, 32, 102, 317, 356, 390, 541, 544
BccI CCATC 2 cut(s) 728, 824
BceAI ACGGC 1 cut(s) 103
BciT130I CCWGG 2 cut(s) 199, 958
BclI TGATCA 2 cut(s) 736, 784
BcoDI GTCTC 2 cut(s) 468, 828
BcuI ACTAGT 1 cut(s) 641
BfaI CTAG 2 cut(s) 642, 863
BfoI RGCGCY 3 cut(s) 64, 437, 683
BfuAI ACCTGC 1 cut(s) 461
BisI GCNGC 9 cut(s) 18, 21, 91, 306, 345, 404, 411, 555, 558
BlsI GCNGC 9 cut(s) 19, 22, 92, 307, 346, 405, 412, 556, 559
Bme1390I CCNGG 2 cut(s) 199, 958
Bme18I GGWCC 1 cut(s) 232
BmgT120I GGNCC 1 cut(s) 232
BmiI GGNNCC 4 cut(s) 138, 390, 511, 681
BmrFI CCNGG 2 cut(s) 199, 958
BmsI GCATC 3 cut(s) 102, 377, 412
BpmI CTGGAG 2 cut(s) 50, 138
Bpu10I CCTNAGC 2 cut(s) 393, 561
BpuEI CTTGAG 1 cut(s) 918
Bsa29I ATCGAT 1 cut(s) 846
BsaBI GATNNNNATC 1 cut(s) 30
BsaHI GRCGYC 3 cut(s) 120, 351, 680
BsaI GGTCTC 1 cut(s) 828
BsaJI CCNNGG 2 cut(s) 277, 957
BsaXI ACNNNNNCTCC 2 cut(s) 198, 228
Bsc4I CCNNNNNNNGG 8 cut(s) 41, 229, 406, 519, 623, 676, 761, 957
Bse118I RCCGGY 3 cut(s) 347, 399, 406
Bse1I ACTGG 4 cut(s) 155, 234, 446, 513
Bse8I GATNNNNATC 1 cut(s) 30
BseBI CCWGG 2 cut(s) 199, 958
BseCI ATCGAT 1 cut(s) 846
BseDI CCNNGG 2 cut(s) 277, 957
BseGI GGATG 2 cut(s) 171, 967
BseJI GATNNNNATC 1 cut(s) 30
BseLI CCNNNNNNNGG 8 cut(s) 41, 229, 406, 519, 623, 676, 761, 957
BseMII CTCAG 2 cut(s) 384, 552
BseNI ACTGG 4 cut(s) 155, 234, 446, 513
BseXI GCAGC 8 cut(s) 29, 32, 102, 317, 356, 390, 541, 544
BseYI CCCAGC 1 cut(s) 518
Bsh1236I CGCG 1 cut(s) 100
Bsh1285I CGRYCG 1 cut(s) 850
BshNI GGYRCC 1 cut(s) 679
BshVI ATCGAT 1 cut(s) 846
BsiEI CGRYCG 1 cut(s) 850
BsiHKAI GWGCWC 1 cut(s) 399
BsiSI CCGG 4 cut(s) 42, 348, 400, 407
BslFI GGGAC 3 cut(s) 338, 558, 719
BslI CCNNNNNNNGG 8 cut(s) 41, 229, 406, 519, 623, 676, 761, 957
BsmAI GTCTC 2 cut(s) 468, 828
BsmFI GGGAC 3 cut(s) 338, 558, 719
Bso31I GGTCTC 1 cut(s) 828
Bsp1286I GDGCHC 2 cut(s) 399, 934
Bsp1407I TGTACA 1 cut(s) 889
Bsp19I CCATGG 1 cut(s) 277
BspACI CCGC 6 cut(s) 23, 125, 220, 410, 542, 761
BspCNI CTCAG 2 cut(s) 385, 553
BspDI ATCGAT 1 cut(s) 846
BspFNI CGCG 1 cut(s) 100
BspLI GGNNCC 4 cut(s) 138, 390, 511, 681
BspMI ACCTGC 1 cut(s) 461
BspPI GGATC 7 cut(s) 26, 44, 131, 144, 452, 504, 517
BspT107I GGYRCC 1 cut(s) 679
BspTNI GGTCTC 1 cut(s) 828
BsrFI RCCGGY 3 cut(s) 347, 399, 406
BsrGI TGTACA 1 cut(s) 889
BsrI ACTGG 4 cut(s) 155, 234, 446, 513
BssAI RCCGGY 3 cut(s) 347, 399, 406
BssECI CCNNGG 2 cut(s) 277, 957
BssNI GRCGYC 3 cut(s) 120, 351, 680
BssSI CACGAG 1 cut(s) 740
BssT1I CCWWGG 1 cut(s) 277
Bst2BI CACGAG 1 cut(s) 740
Bst2UI CCWGG 2 cut(s) 199, 958
Bst4CI ACNGT 3 cut(s) 210, 618, 734
BstACI GRCGYC 3 cut(s) 120, 351, 680
BstAPI GCANNNNNTGC 1 cut(s) 403
BstAUI TGTACA 1 cut(s) 889
BstC8I GCNNGC 3 cut(s) 76, 349, 408
BstDEI CTNAG 3 cut(s) 299, 393, 561
BstDSI CCRYGG 1 cut(s) 277
BstF5I GGATG 2 cut(s) 171, 967
BstFNI CGCG 1 cut(s) 100
BstH2I RGCGCY 3 cut(s) 64, 437, 683
BstHHI GCGC 5 cut(s) 63, 102, 305, 436, 682
BstMAI GTCTC 2 cut(s) 468, 828
BstMCI CGRYCG 1 cut(s) 850
BstMWI GCNNNNNNNGC 4 cut(s) 99, 403, 431, 539
BstNI CCWGG 2 cut(s) 199, 958
BstNSI RCATGY 1 cut(s) 896
BstSCI CCNGG 2 cut(s) 197, 956
BstUI CGCG 1 cut(s) 100
BstV1I GCAGC 8 cut(s) 29, 32, 102, 317, 356, 390, 541, 544
BstX2I RGATCY 2 cut(s) 136, 509
BstYI RGATCY 2 cut(s) 136, 509
Bsu15I ATCGAT 1 cut(s) 846
BsuTUI ATCGAT 1 cut(s) 846
BtgI CCRYGG 1 cut(s) 277
BtsCI GGATG 2 cut(s) 171, 967
BtsI GCAGTG 1 cut(s) 432
BtsIMutI CAGTG 4 cut(s) 432, 439, 520, 839
BveI ACCTGC 1 cut(s) 461
Cac8I GCNNGC 3 cut(s) 76, 349, 408
CfoI GCGC 5 cut(s) 63, 102, 305, 436, 682
Cfr10I RCCGGY 3 cut(s) 347, 399, 406
Cfr13I GGNCC 1 cut(s) 232
ClaI ATCGAT 1 cut(s) 846
CseI GACGC 2 cut(s) 128, 340
Csp6I GTAC 3 cut(s) 227, 379, 890
CviAII CATG 6 cut(s) 224, 278, 323, 700, 893, 926
CviQI GTAC 3 cut(s) 227, 379, 890
DdeI CTNAG 3 cut(s) 299, 393, 561
DinI GGCGCC 1 cut(s) 681
Eco130I CCWWGG 1 cut(s) 277
Eco24I GRGCYC 1 cut(s) 934
Eco31I GGTCTC 1 cut(s) 828
Eco47I GGWCC 1 cut(s) 232
EcoRII CCWGG 2 cut(s) 197, 956
EcoT14I CCWWGG 1 cut(s) 277
EcoT38I GRGCYC 1 cut(s) 934
EgeI GGCGCC 1 cut(s) 681
EheI GGCGCC 1 cut(s) 681
ErhI CCWWGG 1 cut(s) 277
FaeI CATG 6 cut(s) 227, 281, 326, 703, 896, 929
FaqI GGGAC 3 cut(s) 338, 558, 719
FatI CATG 6 cut(s) 223, 277, 322, 699, 892, 925
FauI CCCGC 4 cut(s) 132, 227, 535, 754
FbaI TGATCA 2 cut(s) 736, 784
Fnu4HI GCNGC 9 cut(s) 18, 21, 91, 306, 345, 404, 411, 555, 558
FokI GGATG 2 cut(s) 158, 974
FriOI GRGCYC 1 cut(s) 934
Fsp4HI GCNGC 9 cut(s) 18, 21, 91, 306, 345, 404, 411, 555, 558
FspBI CTAG 2 cut(s) 642, 863
GlaI GCGC 5 cut(s) 62, 101, 304, 435, 681
GluI GCNGC 9 cut(s) 18, 21, 91, 306, 345, 404, 411, 555, 558
GsaI CCCAGC 1 cut(s) 522
GsuI CTGGAG 2 cut(s) 50, 138
HaeII RGCGCY 3 cut(s) 64, 437, 683
HapII CCGG 4 cut(s) 42, 348, 400, 407
HgaI GACGC 2 cut(s) 128, 340
HhaI GCGC 5 cut(s) 63, 102, 305, 436, 682
Hin1I GRCGYC 3 cut(s) 120, 351, 680
Hin1II CATG 6 cut(s) 227, 281, 326, 703, 896, 929
Hin6I GCGC 5 cut(s) 61, 100, 303, 434, 680
HinP1I GCGC 5 cut(s) 61, 100, 303, 434, 680
HindIII AAGCTT 1 cut(s) 143
HinfI GANTC 4 cut(s) 26, 631, 770, 901
HpaII CCGG 4 cut(s) 42, 348, 400, 407
HphI GGTGA 5 cut(s) 154, 234, 673, 829, 910
Hpy166II GTNNAC 2 cut(s) 664, 890
Hpy188I TCNGA 5 cut(s) 31, 136, 649, 829, 855
Hpy188III TCNNGA 6 cut(s) 67, 83, 116, 392, 740, 935
Hpy8I GTNNAC 2 cut(s) 664, 890
Hpy99I CGWCG 3 cut(s) 89, 269, 368
HpyAV CCTTC 3 cut(s) 584, 630, 908
HpyCH4III ACNGT 3 cut(s) 210, 618, 734
HpyCH4V TGCA 4 cut(s) 344, 425, 613, 750
HpyF10VI GCNNNNNNNGC 4 cut(s) 99, 403, 431, 539
HpyF3I CTNAG 3 cut(s) 299, 393, 561
Hsp92I GRCGYC 3 cut(s) 120, 351, 680
Hsp92II CATG 6 cut(s) 227, 281, 326, 703, 896, 929
HspAI GCGC 5 cut(s) 61, 100, 303, 434, 680
KasI GGCGCC 1 cut(s) 679
KroI GCCGGC 2 cut(s) 347, 406
KroNI GCCGGC 2 cut(s) 349, 408
Ksp22I TGATCA 2 cut(s) 736, 784
LmnI GCTCC 2 cut(s) 261, 394
Lsp1109I GCAGC 8 cut(s) 29, 32, 102, 317, 356, 390, 541, 544
LweI GCATC 3 cut(s) 102, 377, 412
MaeI CTAG 2 cut(s) 642, 863
MaeIII GTNAC 1 cut(s) 524
MboII GAAGA 2 cut(s) 601, 880
MflI RGATCY 2 cut(s) 136, 509
MhlI GDGCHC 2 cut(s) 399, 934
Mly113I GGCGCC 1 cut(s) 680
MlyI GAGTC 1 cut(s) 910
MmeI TCCRAC 1 cut(s) 855
MroNI GCCGGC 2 cut(s) 347, 406
MseI TTAA 2 cut(s) 978, 997
MslI CAYNNNNRTG 1 cut(s) 802
MspA1I CMGCKG 1 cut(s) 23
MspI CCGG 4 cut(s) 42, 348, 400, 407
MspR9I CCNGG 2 cut(s) 199, 958
MvaI CCWGG 2 cut(s) 199, 958
MvnI CGCG 1 cut(s) 100
MwoI GCNNNNNNNGC 4 cut(s) 99, 403, 431, 539
NaeI GCCGGC 2 cut(s) 349, 408
NarI GGCGCC 1 cut(s) 680
NcoI CCATGG 1 cut(s) 277
NgoMIV GCCGGC 2 cut(s) 347, 406
NlaIII CATG 6 cut(s) 227, 281, 326, 703, 896, 929
NlaIV GGNNCC 4 cut(s) 138, 390, 511, 681
NspI RCATGY 1 cut(s) 896
OliI CACNNNNGTG 1 cut(s) 802
PciI ACATGT 1 cut(s) 892
PcsI WCGNNNNNNNCGW 1 cut(s) 372
PdiI GCCGGC 2 cut(s) 349, 408
PfeI GAWTC 3 cut(s) 26, 631, 770
PflMI CCANNNNNTGG 1 cut(s) 229
PkrI GCNGC 9 cut(s) 19, 22, 92, 307, 346, 405, 412, 556, 559
Ple19I CGATCG 1 cut(s) 850
PleI GAGTC 1 cut(s) 909
PluTI GGCGCC 1 cut(s) 683
PpsI GAGTC 1 cut(s) 909
PscI ACATGT 1 cut(s) 892
Psp6I CCWGG 2 cut(s) 197, 956
PspFI CCCAGC 1 cut(s) 518
PspGI CCWGG 2 cut(s) 197, 956
PspN4I GGNNCC 4 cut(s) 138, 390, 511, 681
PspPI GGNCC 1 cut(s) 232
PsuI RGATCY 2 cut(s) 136, 509
PvuI CGATCG 1 cut(s) 850
RsaI GTAC 3 cut(s) 228, 380, 891
RsaNI GTAC 3 cut(s) 227, 379, 890
RseI CAYNNNNRTG 1 cut(s) 802
SaqAI TTAA 2 cut(s) 978, 997
SatI GCNGC 9 cut(s) 18, 21, 91, 306, 345, 404, 411, 555, 558
Sau96I GGNCC 1 cut(s) 232
SchI GAGTC 1 cut(s) 910
ScrFI CCNGG 2 cut(s) 199, 958
SduI GDGCHC 2 cut(s) 399, 934
SfaNI GCATC 3 cut(s) 102, 377, 412
SfoI GGCGCC 1 cut(s) 681
SinI GGWCC 1 cut(s) 232
SmiMI CAYNNNNRTG 1 cut(s) 802
SmlI CTYRAG 1 cut(s) 933
SmoI CTYRAG 1 cut(s) 933
SpeI ACTAGT 1 cut(s) 641
SsiI CCGC 6 cut(s) 23, 125, 220, 410, 542, 761
SspDI GGCGCC 1 cut(s) 679
SspMI CTAG 2 cut(s) 642, 863
StyD4I CCNGG 2 cut(s) 197, 956
StyI CCWWGG 1 cut(s) 277
TaaI ACNGT 3 cut(s) 210, 618, 734
TaqI TCGA 4 cut(s) 205, 375, 634, 846
TatI WGTACW 3 cut(s) 226, 378, 889
TauI GCSGC 1 cut(s) 413
TfiI GAWTC 3 cut(s) 26, 631, 770
Tru1I TTAA 2 cut(s) 978, 997
Tru9I TTAA 2 cut(s) 978, 997
TscAI CASTG 4 cut(s) 432, 446, 520, 846
TseI GCWGC 8 cut(s) 17, 20, 90, 305, 344, 403, 554, 557
TspDTI ATGAA 2 cut(s) 24, 280
TspRI CASTG 4 cut(s) 432, 446, 520, 846
Van91I CCANNNNNTGG 1 cut(s) 229
VpaK11BI GGWCC 1 cut(s) 232
XceI RCATGY 1 cut(s) 896
XspI CTAG 2 cut(s) 642, 863
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.