RchiOBHm_Chr1g0376141

Carboxylesterase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0000860
Physical Location & Seq
Forward (+)
63407682 .. 63408530
849 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ59980

Sequence Viewer

Length: 558 bp
ATGGTGGAGGCTTCATATTACTTGGCGCAGCCTCAACAATTTTCCATGCATGATTTCTATTTCAACTTTGCAACCAACGTCCCCGTAATCATCGCATCTGTCGACTACAGGTTGGCTCTTGAGCACCGACTGCCAGCAGCTTATGATGGTGCAGTGGAGGCGCTTCACTGGATCAAAACCAGCCAGGATGATTGTTTGAGAGACTACGCTGACCTCTCCAACTGTTTCCTGTTAAGAGACTACGTGTGGCTGCTGAGGGCGATGACCTTCGTCCACTCGTTGAAGATCGGAGGGCTGATATTGATCCAACAGTTGTTCGGTGGGTCGCGAAGGACTAGCTCGGAGTTGAGGCTAGTGAACGACCCTATTTTGGGGATAAGTGTATGTGATCTCATGTGGGACTTGTCGCTGCCTTTGGGGGTTGACCGTGATCACGAGTATCGCAATCCGATGGCGGGTGGCGATTCTAATGTATTTGATAAAGTGAAAGCACTTGGGTGGAGAGTTTTGGTGAGTGGCTCCGATGGAGACCCACTGATCGACGTCAGATTGAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0009056 GO:0016787
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

185

Amino Acids

20.9

Weight (kDa)

5.07

Isoelectric Point (pI)

39.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydrolase_3 PF07859 15 - 181 1.2e-16 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 546
AccI GTMKAC 1 cut(s) 102
AccII CGCG 1 cut(s) 328
AciI CCGC 1 cut(s) 455
AclWI GGATC 2 cut(s) 179, 298
AcyI GRCGYC 1 cut(s) 543
AfiI CCNNNNNNNGG 3 cut(s) 370, 371, 455
AflIII ACRYGT 1 cut(s) 243
AgsI TTSAA 2 cut(s) 64, 283
AjnI CCWGG 1 cut(s) 183
AleI CACNNNNGTG 1 cut(s) 496
AluBI AGCT 3 cut(s) 140, 339, 555
AluI AGCT 3 cut(s) 140, 339, 555
Alw21I GWGCWC 1 cut(s) 126
Alw26I GTCTC 3 cut(s) 195, 231, 522
AlwI GGATC 2 cut(s) 179, 298
ApeKI GCWGC 4 cut(s) 28, 137, 250, 409
AspLEI GCGC 2 cut(s) 28, 163
AsuHPI GGTGA 1 cut(s) 523
BauI CACGAG 1 cut(s) 434
Bbv12I GWGCWC 1 cut(s) 126
BbvCI CCTCAGC 1 cut(s) 254
BbvI GCAGC 4 cut(s) 40, 149, 237, 396
BccI CCATC 3 cut(s) 140, 445, 518
BciT130I CCWGG 1 cut(s) 185
BclI TGATCA 1 cut(s) 430
BcoDI GTCTC 3 cut(s) 195, 231, 522
BfaI CTAG 2 cut(s) 336, 353
BfmI CTRYAG 1 cut(s) 106
BfoI RGCGCY 1 cut(s) 164
BisI GCNGC 4 cut(s) 29, 138, 251, 410
BlsI GCNGC 4 cut(s) 30, 139, 252, 411
Bme1390I CCNGG 1 cut(s) 185
BmiI GGNNCC 1 cut(s) 520
BmrFI CCNGG 1 cut(s) 185
BmsI GCATC 1 cut(s) 104
BoxI GACNNNNGTC 1 cut(s) 269
Bpu10I CCTNAGC 1 cut(s) 254
BpuEI CTTGAG 1 cut(s) 140
BsaAI YACGTR 1 cut(s) 244
BsaBI GATNNNNATC 1 cut(s) 302
BsaHI GRCGYC 1 cut(s) 543
BsaI GGTCTC 1 cut(s) 522
Bsc4I CCNNNNNNNGG 3 cut(s) 370, 371, 455
Bse1I ACTGG 1 cut(s) 173
Bse8I GATNNNNATC 1 cut(s) 302
BseBI CCWGG 1 cut(s) 185
BseGI GGATG 1 cut(s) 193
BseJI GATNNNNATC 1 cut(s) 302
BseLI CCNNNNNNNGG 3 cut(s) 370, 371, 455
BseMII CTCAG 1 cut(s) 245
BseNI ACTGG 1 cut(s) 173
BseXI GCAGC 4 cut(s) 40, 149, 237, 396
BsgI GTGCAG 1 cut(s) 171
Bsh1236I CGCG 1 cut(s) 328
BsiHKAI GWGCWC 1 cut(s) 126
BslFI GGGAC 2 cut(s) 65, 413
BslI CCNNNNNNNGG 3 cut(s) 370, 371, 455
BsmAI GTCTC 3 cut(s) 195, 231, 522
BsmFI GGGAC 2 cut(s) 65, 413
Bso31I GGTCTC 1 cut(s) 522
Bsp1286I GDGCHC 1 cut(s) 126
Bsp143I GATC 6 cut(s) 171, 285, 303, 388, 430, 537
Bsp68I TCGCGA 1 cut(s) 328
BspACI CCGC 1 cut(s) 455
BspCNI CTCAG 1 cut(s) 246
BspFNI CGCG 1 cut(s) 328
BspLI GGNNCC 1 cut(s) 520
BspPI GGATC 2 cut(s) 179, 298
BspTNI GGTCTC 1 cut(s) 522
BsrI ACTGG 1 cut(s) 173
BssMI GATC 6 cut(s) 171, 285, 303, 388, 430, 537
BssNI GRCGYC 1 cut(s) 543
BssSI CACGAG 1 cut(s) 434
Bst2BI CACGAG 1 cut(s) 434
Bst2UI CCWGG 1 cut(s) 185
Bst4CI ACNGT 3 cut(s) 224, 312, 428
BstACI GRCGYC 1 cut(s) 543
BstAPI GCANNNNNTGC 1 cut(s) 130
BstBAI YACGTR 1 cut(s) 244
BstC8I GCNNGC 1 cut(s) 135
BstDEI CTNAG 1 cut(s) 254
BstF5I GGATG 1 cut(s) 193
BstFNI CGCG 1 cut(s) 328
BstH2I RGCGCY 1 cut(s) 164
BstHHI GCGC 2 cut(s) 28, 163
BstKTI GATC 6 cut(s) 174, 288, 306, 391, 433, 540
BstMAI GTCTC 3 cut(s) 195, 231, 522
BstMBI GATC 6 cut(s) 171, 285, 303, 388, 430, 537
BstMWI GCNNNNNNNGC 2 cut(s) 130, 158
BstNI CCWGG 1 cut(s) 185
BstPAI GACNNNNGTC 1 cut(s) 269
BstSCI CCNGG 1 cut(s) 183
BstSFI CTRYAG 1 cut(s) 106
BstUI CGCG 1 cut(s) 328
BstV1I GCAGC 4 cut(s) 40, 149, 237, 396
BtgZI GCGATG 2 cut(s) 76, 275
BtsCI GGATG 1 cut(s) 193
BtsI GCAGTG 1 cut(s) 159
BtsIMutI CAGTG 3 cut(s) 159, 166, 533
BtuMI TCGCGA 1 cut(s) 328
Cac8I GCNNGC 1 cut(s) 135
CfoI GCGC 2 cut(s) 28, 163
CviAII CATG 3 cut(s) 46, 50, 394
DdeI CTNAG 1 cut(s) 254
DpnI GATC 6 cut(s) 173, 287, 305, 390, 432, 539
DpnII GATC 6 cut(s) 171, 285, 303, 388, 430, 537
Eco31I GGTCTC 1 cut(s) 522
EcoRII CCWGG 1 cut(s) 183
EcoT22I ATGCAT 1 cut(s) 51
FaeI CATG 3 cut(s) 49, 53, 397
FaiI YATR 6 cut(s) 16, 47, 51, 144, 385, 395
FaqI GGGAC 2 cut(s) 65, 413
FatI CATG 3 cut(s) 45, 49, 393
FauI CCCGC 1 cut(s) 448
FbaI TGATCA 1 cut(s) 430
FblI GTMKAC 1 cut(s) 102
Fnu4HI GCNGC 4 cut(s) 29, 138, 251, 410
FokI GGATG 1 cut(s) 200
Fsp4HI GCNGC 4 cut(s) 29, 138, 251, 410
FspBI CTAG 2 cut(s) 336, 353
GlaI GCGC 2 cut(s) 27, 162
GluI GCNGC 4 cut(s) 29, 138, 251, 410
HaeII RGCGCY 1 cut(s) 164
HhaI GCGC 2 cut(s) 28, 163
Hin1I GRCGYC 1 cut(s) 543
Hin1II CATG 3 cut(s) 49, 53, 397
Hin6I GCGC 2 cut(s) 26, 161
HinP1I GCGC 2 cut(s) 26, 161
HincII GTYRAC 2 cut(s) 103, 424
HindII GTYRAC 2 cut(s) 103, 424
HinfI GANTC 1 cut(s) 464
HphI GGTGA 1 cut(s) 523
Hpy166II GTNNAC 4 cut(s) 103, 274, 358, 424
Hpy188I TCNGA 5 cut(s) 290, 343, 450, 523, 548
Hpy188III TCNNGA 3 cut(s) 119, 327, 434
Hpy8I GTNNAC 4 cut(s) 103, 274, 358, 424
Hpy99I CGWCG 1 cut(s) 545
HpyAV CCTTC 2 cut(s) 277, 324
HpyCH4III ACNGT 3 cut(s) 224, 312, 428
HpyCH4IV ACGT 3 cut(s) 78, 243, 543
HpyCH4V TGCA 3 cut(s) 49, 71, 152
HpyF10VI GCNNNNNNNGC 2 cut(s) 130, 158
HpyF3I CTNAG 1 cut(s) 254
HpySE526I ACGT 3 cut(s) 78, 243, 543
Hsp92I GRCGYC 1 cut(s) 543
Hsp92II CATG 3 cut(s) 49, 53, 397
HspAI GCGC 2 cut(s) 26, 161
Ksp22I TGATCA 1 cut(s) 430
Kzo9I GATC 6 cut(s) 171, 285, 303, 388, 430, 537
LmnI GCTCC 1 cut(s) 524
LpnPI CCDG 7 cut(s) 94, 147, 154, 170, 193, 197, 242
Lsp1109I GCAGC 4 cut(s) 40, 149, 237, 396
LweI GCATC 1 cut(s) 104
MaeI CTAG 2 cut(s) 336, 353
MaeII ACGT 3 cut(s) 78, 243, 543
MalI GATC 6 cut(s) 173, 287, 305, 390, 432, 539
MboI GATC 6 cut(s) 171, 285, 303, 388, 430, 537
MboII GAAGA 1 cut(s) 295
MhlI GDGCHC 1 cut(s) 126
MluCI AATT 1 cut(s) 38
MmeI TCCRAC 2 cut(s) 243, 331
MnlI CCTC 6 cut(s) 42, 151, 224, 249, 284, 342
Mph1103I ATGCAT 1 cut(s) 51
MseI TTAA 1 cut(s) 233
MslI CAYNNNNRTG 1 cut(s) 496
MspR9I CCNGG 1 cut(s) 185
MvaI CCWGG 1 cut(s) 185
MvnI CGCG 1 cut(s) 328
MwoI GCNNNNNNNGC 2 cut(s) 130, 158
NdeII GATC 6 cut(s) 171, 285, 303, 388, 430, 537
NlaIII CATG 3 cut(s) 49, 53, 397
NlaIV GGNNCC 1 cut(s) 520
NruI TCGCGA 1 cut(s) 328
NsiI ATGCAT 1 cut(s) 51
OliI CACNNNNGTG 1 cut(s) 496
PcsI WCGNNNNNNNCGW 1 cut(s) 99
PfeI GAWTC 1 cut(s) 464
PkrI GCNGC 4 cut(s) 30, 139, 252, 411
Ppu21I YACGTR 1 cut(s) 244
PshAI GACNNNNGTC 1 cut(s) 269
Psp6I CCWGG 1 cut(s) 183
PspGI CCWGG 1 cut(s) 183
PspN4I GGNNCC 1 cut(s) 520
RruI TCGCGA 1 cut(s) 328
RseI CAYNNNNRTG 1 cut(s) 496
SalI GTCGAC 1 cut(s) 101
SaqAI TTAA 1 cut(s) 233
SatI GCNGC 4 cut(s) 29, 138, 251, 410
Sau3AI GATC 6 cut(s) 171, 285, 303, 388, 430, 537
ScrFI CCNGG 1 cut(s) 185
SduI GDGCHC 1 cut(s) 126
SetI ASST 9 cut(s) 81, 113, 142, 216, 246, 269, 341, 546, 557
SfaNI GCATC 1 cut(s) 104
SfcI CTRYAG 1 cut(s) 106
SmiMI CAYNNNNRTG 1 cut(s) 496
SmlI CTYRAG 1 cut(s) 119
SmoI CTYRAG 1 cut(s) 119
Sse9I AATT 1 cut(s) 38
SsiI CCGC 1 cut(s) 455
SspMI CTAG 2 cut(s) 336, 353
StyD4I CCNGG 1 cut(s) 183
TaaI ACNGT 3 cut(s) 224, 312, 428
TaiI ACGT 3 cut(s) 81, 246, 546
TaqI TCGA 2 cut(s) 102, 540
TasI AATT 1 cut(s) 38
TfiI GAWTC 1 cut(s) 464
Tru1I TTAA 1 cut(s) 233
Tru9I TTAA 1 cut(s) 233
TscAI CASTG 3 cut(s) 159, 173, 540
TseI GCWGC 4 cut(s) 28, 137, 250, 409
TspRI CASTG 3 cut(s) 159, 173, 540
XmiI GTMKAC 1 cut(s) 102
XspI CTAG 2 cut(s) 336, 353
ZraI GACGTC 1 cut(s) 544
Zsp2I ATGCAT 1 cut(s) 51
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.