Rorug01G0394100

alpha/beta hydrolase fold

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0000860
Physical Location & Seq
Reverse (-)
50432338 .. 50434561
2224 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0394100.1

Sequence Viewer

Length: 255 bp
ATGACTTCGCAGCAGGAAAGGTCTGAGCTGGACGAGAGGGCCAGGCGAGGAGAGACTGTGATCCCCGGCGGGACTGGCGGCAAAAGCCTTGAAGCTCAGGAACACCTTGCTGAAGGGAGGAGCAAAGGAGGGCAGACGAGGAAGGAGCAGATAGGGCAGGAAGGGTACCAGGAAATGGGCCGCAAGGGAGGGCTGAGCACTGGGGACGAGTCCGGCGGAGAACGCGCCGCTCGGGATCGAGGGTATTCCGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

84

Amino Acids

9.04

Weight (kDa)

5.48

Isoelectric Point (pI)

40.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LEA_5 PF00477 2 - 56 1.1e-25 Small hydrophilic plant seed protein
LEA_5 PF00477 41 - 79 6.5e-16 Small hydrophilic plant seed protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 165
AccB1I GGYRCC 1 cut(s) 165
AccB7I CCANNNNNTGG 1 cut(s) 175
AccBSI CCGCTC 1 cut(s) 230
AccII CGCG 1 cut(s) 225
AciI CCGC 5 cut(s) 69, 78, 181, 216, 228
AclWI GGATC 2 cut(s) 55, 243
AcuI CTGAAG 1 cut(s) 132
AfaI GTAC 1 cut(s) 167
AfiI CCNNNNNNNGG 1 cut(s) 175
AgsI TTSAA 1 cut(s) 92
AjnI CCWGG 2 cut(s) 41, 168
AluBI AGCT 2 cut(s) 28, 95
AluI AGCT 2 cut(s) 28, 95
Alw21I GWGCWC 1 cut(s) 200
Alw26I GTCTC 1 cut(s) 47
AlwI GGATC 2 cut(s) 55, 243
Ama87I CYCGRG 1 cut(s) 231
AoxI GGCC 2 cut(s) 39, 178
ApeKI GCWGC 1 cut(s) 10
Asp718I GGTACC 1 cut(s) 165
AspLEI GCGC 1 cut(s) 227
AspS9I GGNCC 2 cut(s) 39, 178
AsuC2I CCSGG 1 cut(s) 66
AvaI CYCGRG 1 cut(s) 231
BanI GGYRCC 1 cut(s) 165
Bbv12I GWGCWC 1 cut(s) 200
BbvI GCAGC 1 cut(s) 22
BciT130I CCWGG 2 cut(s) 43, 170
BcnI CCSGG 1 cut(s) 66
BcoDI GTCTC 1 cut(s) 47
BisI GCNGC 4 cut(s) 11, 79, 181, 228
BlpI GCTNAGC 1 cut(s) 194
BlsI GCNGC 4 cut(s) 12, 80, 182, 229
Bme1390I CCNGG 3 cut(s) 43, 66, 170
BmeT110I CYCGRG 1 cut(s) 231
BmgT120I GGNCC 2 cut(s) 39, 178
BmiI GGNNCC 1 cut(s) 167
BmrFI CCNGG 3 cut(s) 43, 66, 170
BmrI ACTGGG 1 cut(s) 210
BmuI ACTGGG 1 cut(s) 210
Bpu10I CCTNAGC 1 cut(s) 96
Bpu1102I GCTNAGC 1 cut(s) 194
BpuMI CCSGG 1 cut(s) 66
BsaJI CCNNGG 1 cut(s) 64
BsaXI ACNNNNNCTCC 2 cut(s) 42, 72
Bsc4I CCNNNNNNNGG 1 cut(s) 175
Bse1I ACTGG 2 cut(s) 79, 205
BseBI CCWGG 2 cut(s) 43, 170
BseDI CCNNGG 1 cut(s) 64
BseLI CCNNNNNNNGG 1 cut(s) 175
BseMII CTCAG 3 cut(s) 15, 110, 185
BseNI ACTGG 2 cut(s) 79, 205
BseRI GAGGAG 2 cut(s) 63, 133
BseXI GCAGC 1 cut(s) 22
Bsh1236I CGCG 1 cut(s) 225
BshFI GGCC 2 cut(s) 41, 180
BshNI GGYRCC 1 cut(s) 165
BsiHKAI GWGCWC 1 cut(s) 200
BsiHKCI CYCGRG 1 cut(s) 231
BsiSI CCGG 2 cut(s) 66, 213
BslFI GGGAC 2 cut(s) 85, 218
BslI CCNNNNNNNGG 1 cut(s) 175
BsmAI GTCTC 1 cut(s) 47
BsmFI GGGAC 2 cut(s) 85, 218
BsnI GGCC 2 cut(s) 41, 180
BsoBI CYCGRG 1 cut(s) 231
Bsp1286I GDGCHC 1 cut(s) 200
Bsp143I GATC 2 cut(s) 60, 235
Bsp1720I GCTNAGC 1 cut(s) 194
BspACI CCGC 5 cut(s) 69, 78, 181, 216, 228
BspANI GGCC 2 cut(s) 41, 180
BspCNI CTCAG 3 cut(s) 16, 109, 186
BspFNI CGCG 1 cut(s) 225
BspLI GGNNCC 1 cut(s) 167
BspPI GGATC 2 cut(s) 55, 243
BspT107I GGYRCC 1 cut(s) 165
BsrBI CCGCTC 1 cut(s) 230
BsrI ACTGG 2 cut(s) 79, 205
BssECI CCNNGG 1 cut(s) 64
BssMI GATC 2 cut(s) 60, 235
Bst2UI CCWGG 2 cut(s) 43, 170
Bst4CI ACNGT 1 cut(s) 58
BstDEI CTNAG 3 cut(s) 24, 96, 194
BstFNI CGCG 1 cut(s) 225
BstHHI GCGC 1 cut(s) 227
BstKTI GATC 2 cut(s) 63, 238
BstMAI GTCTC 1 cut(s) 47
BstMBI GATC 2 cut(s) 60, 235
BstMWI GCNNNNNNNGC 4 cut(s) 75, 84, 154, 222
BstNI CCWGG 2 cut(s) 43, 170
BstSCI CCNGG 3 cut(s) 41, 64, 168
BstUI CGCG 1 cut(s) 225
BstV1I GCAGC 1 cut(s) 22
BsuRI GGCC 2 cut(s) 41, 180
BtsIMutI CAGTG 1 cut(s) 198
CfoI GCGC 1 cut(s) 227
Cfr13I GGNCC 2 cut(s) 39, 178
Csp6I GTAC 1 cut(s) 166
CviJI RGCY 6 cut(s) 28, 41, 87, 95, 180, 193
CviKI_1 RGCY 6 cut(s) 28, 41, 87, 95, 180, 193
CviQI GTAC 1 cut(s) 166
DdeI CTNAG 3 cut(s) 24, 96, 194
DpnI GATC 2 cut(s) 62, 237
DpnII GATC 2 cut(s) 60, 235
EciI GGCGGA 1 cut(s) 231
Eco57I CTGAAG 1 cut(s) 132
Eco88I CYCGRG 1 cut(s) 231
EcoRII CCWGG 2 cut(s) 41, 168
FaqI GGGAC 2 cut(s) 85, 218
FauI CCCGC 1 cut(s) 62
Fnu4HI GCNGC 4 cut(s) 11, 79, 181, 228
Fsp4HI GCNGC 4 cut(s) 11, 79, 181, 228
GlaI GCGC 1 cut(s) 226
GluI GCNGC 4 cut(s) 11, 79, 181, 228
HaeIII GGCC 2 cut(s) 41, 180
HapII CCGG 2 cut(s) 66, 213
HhaI GCGC 1 cut(s) 227
Hin6I GCGC 1 cut(s) 225
HinP1I GCGC 1 cut(s) 225
HinfI GANTC 1 cut(s) 209
HpaII CCGG 2 cut(s) 66, 213
Hpy188I TCNGA 2 cut(s) 25, 250
Hpy188III TCNNGA 2 cut(s) 98, 233
HpyAV CCTTC 3 cut(s) 107, 136, 155
HpyCH4III ACNGT 1 cut(s) 58
HpyF10VI GCNNNNNNNGC 4 cut(s) 75, 84, 154, 222
HpyF3I CTNAG 3 cut(s) 24, 96, 194
HspAI GCGC 1 cut(s) 225
KpnI GGTACC 1 cut(s) 169
Kzo9I GATC 2 cut(s) 60, 235
LmnI GCTCC 2 cut(s) 120, 145
Lsp1109I GCAGC 1 cut(s) 22
MalI GATC 2 cut(s) 62, 237
MbiI CCGCTC 1 cut(s) 230
MboI GATC 2 cut(s) 60, 235
MhlI GDGCHC 1 cut(s) 200
MlyI GAGTC 1 cut(s) 218
MnlI CCTC 7 cut(s) 30, 41, 111, 122, 132, 182, 233
MspI CCGG 2 cut(s) 66, 213
MspR9I CCNGG 3 cut(s) 43, 66, 170
MvaI CCWGG 2 cut(s) 43, 170
MvnI CGCG 1 cut(s) 225
MwoI GCNNNNNNNGC 4 cut(s) 75, 84, 154, 222
NciI CCSGG 1 cut(s) 66
NdeII GATC 2 cut(s) 60, 235
NlaIV GGNNCC 1 cut(s) 167
PflMI CCANNNNNTGG 1 cut(s) 175
PkrI GCNGC 4 cut(s) 12, 80, 182, 229
PleI GAGTC 1 cut(s) 217
PpsI GAGTC 1 cut(s) 217
Psp6I CCWGG 2 cut(s) 41, 168
PspGI CCWGG 2 cut(s) 41, 168
PspN4I GGNNCC 1 cut(s) 167
PspPI GGNCC 2 cut(s) 39, 178
RsaI GTAC 1 cut(s) 167
RsaNI GTAC 1 cut(s) 166
SatI GCNGC 4 cut(s) 11, 79, 181, 228
Sau3AI GATC 2 cut(s) 60, 235
Sau96I GGNCC 2 cut(s) 39, 178
SchI GAGTC 1 cut(s) 218
ScrFI CCNGG 3 cut(s) 43, 66, 170
SduI GDGCHC 1 cut(s) 200
SetI ASST 4 cut(s) 23, 30, 97, 108
SsiI CCGC 5 cut(s) 69, 78, 181, 216, 228
StyD4I CCNGG 3 cut(s) 41, 64, 168
TaaI ACNGT 1 cut(s) 58
TaqI TCGA 1 cut(s) 238
TauI GCSGC 3 cut(s) 81, 183, 230
TscAI CASTG 1 cut(s) 205
TseI GCWGC 1 cut(s) 10
TspRI CASTG 1 cut(s) 205
Van91I CCANNNNNTGG 1 cut(s) 175
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.