Rw1G036260

Carboxylesterase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0000860
Physical Location & Seq
Reverse (-)
64159738 .. 64161970
2233 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G036260.1

Sequence Viewer

Length: 1128 bp
ATGGAGCGTGGAAATTGGCGTTGTCTATTGGTGCTGACCGTGATCATGAGTATTAATTGCAATCCAACGGTGGGTGATAGAGATGATACTTGGGCTGCGATTCGGGCCATGGATTGTAGGGTCTTGGTTTTGGGTTGTTATGGAGATCCGATGATTGAGCGACAGATGGTGTTGGCGAAAATGGTAGAGAAGAAGGGTGTTCGGACGGCTAGGGCAGCCGCGGATTCCGATCCCAACTCCGGCGTTGATCCTTACCAGCGCCTCCAGATAGCACGCAATCCCGACGGCAACGCCCCGCCCTCATCTGATCCCAAGCTTCCCACTCCAGTTCTCACCAAAGACATCCCCATCAACCAATCAAATAATACCTGGCTTCGACTGTTCCTCCCCCGCCATGTACTGGACCGAACCTCACCAACTACCAAGCTCCCTCTCGTCGTTTATTACCATGGTGGAGGCTTCATATTACTTAGCGCAGCCTCAACCATTTTCCATGATTTCTGTTTCAGCTTTGCAGCCGGTGTCCCCGTGATCGTCGCATCTGTCGAGTACAGGTTGGCTCCTGAGCACCGGCTGCCGGCGGCTTATGATGATGCAGTGGAGGCGCTTCACTGGATCAAAACCTGCCAAGATGATTGGTTGAGAGACTATGCTGACCTCTTCACTTGTTTCCTTATGGGATCCAGTGCTGGGGGCATTGACCTTCTCCACCTGTTGAAGATTAGAGGCCTGATATTGGTGCAACCGTATTTCGGGGGGAATCGAAGGACTAGTTCAGAGTTGAGGTTGGTGAACAACTCTGTTTTGGCGCCAAGTGTCTGTGATCCCATCTGGGACTTGTCGTTGCCTATCGGGGCTGACCGTGATCACGAGTATTGCAATCCAATGGCGGGTGGCGATTCTAATGGCTTTGATCAAGTGAAAGCACTTGGGTGGAAAGTTTTGGTGAGTGGCTCTGATGGAGACCCACTGATCGATCGTCAGATTGAGCTGGTGAAGATGTTGGAGGGAAAGGGTGTACATGTGGTGAGTCAGTTTATTGAAGGAGGTTATCATGGGGCTCAAGATGTTGAACCCTCCAAAGCCAGGGATGTGTTTGTGATTGTTAAGCATTTCATATTTCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0009056 GO:0016787
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

375

Amino Acids

41.43

Weight (kDa)

5.93

Isoelectric Point (pI)

33.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BD-FAE PF20434 140 - 237 2.5e-09 BD-FAE
Abhydrolase_3 PF07859 146 - 354 1.2e-41 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 632
AccB1I GGYRCC 1 cut(s) 808
AccB7I CCANNNNNTGG 1 cut(s) 400
AccII CGCG 1 cut(s) 221
AciI CCGC 6 cut(s) 219, 221, 296, 391, 581, 890
AclWI GGATC 8 cut(s) 140, 224, 242, 302, 623, 675, 688, 818
AcyI GRCGYC 1 cut(s) 809
AfaI GTAC 3 cut(s) 399, 551, 1020
AfiI CCNNNNNNNGG 9 cut(s) 71, 239, 400, 577, 690, 736, 752, 890, 1086
AflIII ACRYGT 1 cut(s) 1021
AgsI TTSAA 3 cut(s) 718, 1043, 1073
AhlI ACTAGT 1 cut(s) 770
AjnI CCWGG 2 cut(s) 368, 1085
AleI CACNNNNGTG 1 cut(s) 931
AluBI AGCT 4 cut(s) 316, 427, 510, 991
AluI AGCT 4 cut(s) 316, 427, 510, 991
Alw21I GWGCWC 1 cut(s) 570
Alw26I GTCTC 2 cut(s) 639, 957
AlwI GGATC 8 cut(s) 140, 224, 242, 302, 623, 675, 688, 818
AoxI GGCC 2 cut(s) 105, 727
ApeKI GCWGC 5 cut(s) 95, 215, 476, 515, 574
AseI ATTAAT 1 cut(s) 54
AspLEI GCGC 4 cut(s) 261, 476, 607, 811
AspS9I GGNCC 2 cut(s) 105, 403
AsuHPI GGTGA 7 cut(s) 86, 325, 405, 802, 958, 1006, 1039
AvaII GGWCC 1 cut(s) 403
BamHI GGATCC 1 cut(s) 680
BanI GGYRCC 1 cut(s) 808
BanII GRGCYC 1 cut(s) 1063
BauI CACGAG 1 cut(s) 869
Bbv12I GWGCWC 1 cut(s) 570
BbvI GCAGC 5 cut(s) 82, 227, 488, 527, 561
BccI CCATC 4 cut(s) 160, 356, 836, 953
BceAI ACGGC 2 cut(s) 222, 301
BciT130I CCWGG 2 cut(s) 370, 1087
BclI TGATCA 3 cut(s) 42, 865, 913
BcoDI GTCTC 2 cut(s) 639, 957
BcuI ACTAGT 1 cut(s) 770
BfaI CTAG 2 cut(s) 210, 771
BfoI RGCGCY 3 cut(s) 262, 608, 812
BfuAI ACCTGC 1 cut(s) 632
BisI GCNGC 7 cut(s) 96, 216, 219, 477, 516, 575, 582
BlsI GCNGC 7 cut(s) 97, 217, 220, 478, 517, 576, 583
Bme1390I CCNGG 2 cut(s) 370, 1087
Bme18I GGWCC 1 cut(s) 403
BmgT120I GGNCC 2 cut(s) 105, 403
BmiI GGNNCC 3 cut(s) 561, 682, 810
BmrFI CCNGG 2 cut(s) 370, 1087
BmsI GCATC 2 cut(s) 548, 583
BpmI CTGGAG 2 cut(s) 248, 309
Bpu10I CCTNAGC 1 cut(s) 564
BpuEI CTTGAG 1 cut(s) 1047
Bsa29I ATCGAT 1 cut(s) 975
BsaBI GATNNNNATC 1 cut(s) 228
BsaHI GRCGYC 1 cut(s) 809
BsaI GGTCTC 1 cut(s) 957
BsaJI CCNNGG 4 cut(s) 108, 219, 448, 1086
BsaXI ACNNNNNCTCC 2 cut(s) 369, 399
Bsc4I CCNNNNNNNGG 9 cut(s) 71, 239, 400, 577, 690, 736, 752, 890, 1086
Bse118I RCCGGY 3 cut(s) 518, 570, 577
Bse1I ACTGG 4 cut(s) 326, 405, 617, 684
Bse8I GATNNNNATC 1 cut(s) 228
BseBI CCWGG 2 cut(s) 370, 1087
BseCI ATCGAT 1 cut(s) 975
BseDI CCNNGG 4 cut(s) 108, 219, 448, 1086
BseGI GGATG 2 cut(s) 342, 1096
BseJI GATNNNNATC 1 cut(s) 228
BseLI CCNNNNNNNGG 9 cut(s) 71, 239, 400, 577, 690, 736, 752, 890, 1086
BseMII CTCAG 1 cut(s) 555
BseNI ACTGG 4 cut(s) 326, 405, 617, 684
BseXI GCAGC 5 cut(s) 82, 227, 488, 527, 561
BseYI CCCAGC 1 cut(s) 689
Bsh1236I CGCG 1 cut(s) 221
Bsh1285I CGRYCG 1 cut(s) 979
BshFI GGCC 2 cut(s) 107, 729
BshNI GGYRCC 1 cut(s) 808
BshVI ATCGAT 1 cut(s) 975
BsiEI CGRYCG 1 cut(s) 979
BsiHKAI GWGCWC 1 cut(s) 570
BsiSI CCGG 4 cut(s) 240, 519, 571, 578
BslFI GGGAC 2 cut(s) 509, 848
BslI CCNNNNNNNGG 9 cut(s) 71, 239, 400, 577, 690, 736, 752, 890, 1086
BsmAI GTCTC 2 cut(s) 639, 957
BsmFI GGGAC 2 cut(s) 509, 848
BsnI GGCC 2 cut(s) 107, 729
Bso31I GGTCTC 1 cut(s) 957
Bsp1286I GDGCHC 2 cut(s) 570, 1063
Bsp1407I TGTACA 1 cut(s) 1018
Bsp19I CCATGG 2 cut(s) 108, 448
BspACI CCGC 6 cut(s) 219, 221, 296, 391, 581, 890
BspANI GGCC 2 cut(s) 107, 729
BspCNI CTCAG 1 cut(s) 556
BspDI ATCGAT 1 cut(s) 975
BspFNI CGCG 1 cut(s) 221
BspHI TCATGA 1 cut(s) 45
BspLI GGNNCC 3 cut(s) 561, 682, 810
BspMI ACCTGC 1 cut(s) 632
BspPI GGATC 8 cut(s) 140, 224, 242, 302, 623, 675, 688, 818
BspT107I GGYRCC 1 cut(s) 808
BspTNI GGTCTC 1 cut(s) 957
BsrFI RCCGGY 3 cut(s) 518, 570, 577
BsrGI TGTACA 1 cut(s) 1018
BsrI ACTGG 4 cut(s) 326, 405, 617, 684
BssAI RCCGGY 3 cut(s) 518, 570, 577
BssECI CCNNGG 4 cut(s) 108, 219, 448, 1086
BssNI GRCGYC 1 cut(s) 809
BssSI CACGAG 1 cut(s) 869
BssT1I CCWWGG 2 cut(s) 108, 448
Bst2BI CACGAG 1 cut(s) 869
Bst2UI CCWGG 2 cut(s) 370, 1087
Bst4CI ACNGT 5 cut(s) 40, 70, 381, 747, 863
Bst6I CTCTTC 1 cut(s) 665
BstACI GRCGYC 1 cut(s) 809
BstAPI GCANNNNNTGC 1 cut(s) 574
BstAUI TGTACA 1 cut(s) 1018
BstC8I GCNNGC 2 cut(s) 274, 579
BstDEI CTNAG 2 cut(s) 470, 564
BstDSI CCRYGG 3 cut(s) 108, 219, 448
BstF5I GGATG 2 cut(s) 342, 1096
BstFNI CGCG 1 cut(s) 221
BstH2I RGCGCY 3 cut(s) 262, 608, 812
BstHHI GCGC 4 cut(s) 261, 476, 607, 811
BstMAI GTCTC 2 cut(s) 639, 957
BstMCI CGRYCG 1 cut(s) 979
BstMWI GCNNNNNNNGC 4 cut(s) 104, 215, 574, 602
BstNI CCWGG 2 cut(s) 370, 1087
BstNSI RCATGY 1 cut(s) 1025
BstSCI CCNGG 2 cut(s) 368, 1085
BstUI CGCG 1 cut(s) 221
BstV1I GCAGC 5 cut(s) 82, 227, 488, 527, 561
BstX2I RGATCY 2 cut(s) 145, 680
BstYI RGATCY 2 cut(s) 145, 680
Bsu15I ATCGAT 1 cut(s) 975
BsuRI GGCC 2 cut(s) 107, 729
BsuTUI ATCGAT 1 cut(s) 975
BtgI CCRYGG 3 cut(s) 108, 219, 448
BtsCI GGATG 2 cut(s) 342, 1096
BtsI GCAGTG 1 cut(s) 603
BtsIMutI CAGTG 4 cut(s) 603, 610, 691, 968
BveI ACCTGC 1 cut(s) 632
Cac8I GCNNGC 2 cut(s) 274, 579
CciI TCATGA 1 cut(s) 45
CfoI GCGC 4 cut(s) 261, 476, 607, 811
Cfr10I RCCGGY 3 cut(s) 518, 570, 577
Cfr13I GGNCC 2 cut(s) 105, 403
Cfr42I CCGCGG 1 cut(s) 222
ClaI ATCGAT 1 cut(s) 975
Csp6I GTAC 3 cut(s) 398, 550, 1019
CviAII CATG 7 cut(s) 46, 109, 395, 449, 494, 1022, 1055
CviQI GTAC 3 cut(s) 398, 550, 1019
DdeI CTNAG 2 cut(s) 470, 564
DinI GGCGCC 1 cut(s) 810
Eam1104I CTCTTC 1 cut(s) 665
EarI CTCTTC 1 cut(s) 665
Eco130I CCWWGG 2 cut(s) 108, 448
Eco147I AGGCCT 1 cut(s) 729
Eco24I GRGCYC 1 cut(s) 1063
Eco31I GGTCTC 1 cut(s) 957
Eco47I GGWCC 1 cut(s) 403
EcoRII CCWGG 2 cut(s) 368, 1085
EcoT14I CCWWGG 2 cut(s) 108, 448
EcoT38I GRGCYC 1 cut(s) 1063
EgeI GGCGCC 1 cut(s) 810
EheI GGCGCC 1 cut(s) 810
ErhI CCWWGG 2 cut(s) 108, 448
FaeI CATG 7 cut(s) 49, 112, 398, 452, 497, 1025, 1058
FaqI GGGAC 2 cut(s) 509, 848
FatI CATG 7 cut(s) 45, 108, 394, 448, 493, 1021, 1054
FauI CCCGC 3 cut(s) 303, 398, 883
FbaI TGATCA 3 cut(s) 42, 865, 913
Fnu4HI GCNGC 7 cut(s) 96, 216, 219, 477, 516, 575, 582
FokI GGATG 2 cut(s) 329, 1103
FriOI GRGCYC 1 cut(s) 1063
Fsp4HI GCNGC 7 cut(s) 96, 216, 219, 477, 516, 575, 582
FspBI CTAG 2 cut(s) 210, 771
GlaI GCGC 4 cut(s) 260, 475, 606, 810
GluI GCNGC 7 cut(s) 96, 216, 219, 477, 516, 575, 582
GsaI CCCAGC 1 cut(s) 693
GsuI CTGGAG 2 cut(s) 248, 309
HaeII RGCGCY 3 cut(s) 262, 608, 812
HaeIII GGCC 2 cut(s) 107, 729
HapII CCGG 4 cut(s) 240, 519, 571, 578
HhaI GCGC 4 cut(s) 261, 476, 607, 811
Hin1I GRCGYC 1 cut(s) 809
Hin1II CATG 7 cut(s) 49, 112, 398, 452, 497, 1025, 1058
Hin6I GCGC 4 cut(s) 259, 474, 605, 809
HinP1I GCGC 4 cut(s) 259, 474, 605, 809
HindIII AAGCTT 1 cut(s) 314
HinfI GANTC 5 cut(s) 100, 224, 760, 899, 1030
HpaII CCGG 4 cut(s) 240, 519, 571, 578
HphI GGTGA 7 cut(s) 86, 325, 405, 802, 958, 1006, 1039
Hpy166II GTNNAC 2 cut(s) 793, 1019
Hpy188I TCNGA 7 cut(s) 150, 204, 229, 307, 778, 958, 984
Hpy188III TCNNGA 6 cut(s) 46, 265, 281, 563, 869, 1064
Hpy8I GTNNAC 2 cut(s) 793, 1019
Hpy99I CGWCG 3 cut(s) 287, 440, 539
HpyAV CCTTC 4 cut(s) 187, 713, 759, 1037
HpyCH4III ACNGT 5 cut(s) 40, 70, 381, 747, 863
HpyCH4V TGCA 5 cut(s) 60, 515, 596, 742, 879
HpyF10VI GCNNNNNNNGC 4 cut(s) 104, 215, 574, 602
HpyF3I CTNAG 2 cut(s) 470, 564
Hsp92I GRCGYC 1 cut(s) 809
Hsp92II CATG 7 cut(s) 49, 112, 398, 452, 497, 1025, 1058
HspAI GCGC 4 cut(s) 259, 474, 605, 809
KasI GGCGCC 1 cut(s) 808
KroI GCCGGC 1 cut(s) 577
KroNI GCCGGC 1 cut(s) 579
Ksp22I TGATCA 3 cut(s) 42, 865, 913
KspI CCGCGG 1 cut(s) 222
LmnI GCTCC 3 cut(s) 4, 432, 565
Lsp1109I GCAGC 5 cut(s) 82, 227, 488, 527, 561
LweI GCATC 2 cut(s) 548, 583
MaeI CTAG 2 cut(s) 210, 771
MboII GAAGA 4 cut(s) 202, 652, 730, 1009
MflI RGATCY 2 cut(s) 145, 680
MhlI GDGCHC 2 cut(s) 570, 1063
MluCI AATT 2 cut(s) 13, 55
Mly113I GGCGCC 1 cut(s) 809
MlyI GAGTC 1 cut(s) 1039
MmeI TCCRAC 2 cut(s) 89, 984
MroNI GCCGGC 1 cut(s) 577
MseI TTAA 3 cut(s) 54, 1107, 1126
MslI CAYNNNNRTG 1 cut(s) 931
MspA1I CMGCKG 1 cut(s) 221
MspI CCGG 4 cut(s) 240, 519, 571, 578
MspR9I CCNGG 2 cut(s) 370, 1087
MvaI CCWGG 2 cut(s) 370, 1087
MvnI CGCG 1 cut(s) 221
MwoI GCNNNNNNNGC 4 cut(s) 104, 215, 574, 602
NaeI GCCGGC 1 cut(s) 579
NarI GGCGCC 1 cut(s) 809
NcoI CCATGG 2 cut(s) 108, 448
NgoMIV GCCGGC 1 cut(s) 577
NlaIII CATG 7 cut(s) 49, 112, 398, 452, 497, 1025, 1058
NlaIV GGNNCC 3 cut(s) 561, 682, 810
NspI RCATGY 1 cut(s) 1025
OliI CACNNNNGTG 1 cut(s) 931
PagI TCATGA 1 cut(s) 45
PceI AGGCCT 1 cut(s) 729
PciI ACATGT 1 cut(s) 1021
PcsI WCGNNNNNNNCGW 1 cut(s) 543
PdiI GCCGGC 1 cut(s) 579
PfeI GAWTC 4 cut(s) 100, 224, 760, 899
PflMI CCANNNNNTGG 1 cut(s) 400
PkrI GCNGC 7 cut(s) 97, 217, 220, 478, 517, 576, 583
Ple19I CGATCG 1 cut(s) 979
PleI GAGTC 1 cut(s) 1038
PluTI GGCGCC 1 cut(s) 812
PpsI GAGTC 1 cut(s) 1038
PscI ACATGT 1 cut(s) 1021
PshBI ATTAAT 1 cut(s) 54
Psp6I CCWGG 2 cut(s) 368, 1085
PspFI CCCAGC 1 cut(s) 689
PspGI CCWGG 2 cut(s) 368, 1085
PspN4I GGNNCC 3 cut(s) 561, 682, 810
PspPI GGNCC 2 cut(s) 105, 403
PsuI RGATCY 2 cut(s) 145, 680
PvuI CGATCG 1 cut(s) 979
RsaI GTAC 3 cut(s) 399, 551, 1020
RsaNI GTAC 3 cut(s) 398, 550, 1019
RseI CAYNNNNRTG 1 cut(s) 931
SacII CCGCGG 1 cut(s) 222
SaqAI TTAA 3 cut(s) 54, 1107, 1126
SatI GCNGC 7 cut(s) 96, 216, 219, 477, 516, 575, 582
Sau96I GGNCC 2 cut(s) 105, 403
SchI GAGTC 1 cut(s) 1039
ScrFI CCNGG 2 cut(s) 370, 1087
SduI GDGCHC 2 cut(s) 570, 1063
SfaNI GCATC 2 cut(s) 548, 583
SfoI GGCGCC 1 cut(s) 810
Sfr303I CCGCGG 1 cut(s) 222
SgrBI CCGCGG 1 cut(s) 222
SinI GGWCC 1 cut(s) 403
SmiMI CAYNNNNRTG 1 cut(s) 931
SmlI CTYRAG 1 cut(s) 1062
SmoI CTYRAG 1 cut(s) 1062
SpeI ACTAGT 1 cut(s) 770
Sse9I AATT 2 cut(s) 13, 55
SseBI AGGCCT 1 cut(s) 729
SsiI CCGC 6 cut(s) 219, 221, 296, 391, 581, 890
SspDI GGCGCC 1 cut(s) 808
SspMI CTAG 2 cut(s) 210, 771
StuI AGGCCT 1 cut(s) 729
StyD4I CCNGG 2 cut(s) 368, 1085
StyI CCWWGG 2 cut(s) 108, 448
TaaI ACNGT 5 cut(s) 40, 70, 381, 747, 863
TaqI TCGA 4 cut(s) 376, 546, 763, 975
TaqII GACCGA 1 cut(s) 420
TasI AATT 2 cut(s) 13, 55
TatI WGTACW 3 cut(s) 397, 549, 1018
TauI GCSGC 2 cut(s) 221, 584
TfiI GAWTC 4 cut(s) 100, 224, 760, 899
Tru1I TTAA 3 cut(s) 54, 1107, 1126
Tru9I TTAA 3 cut(s) 54, 1107, 1126
TscAI CASTG 4 cut(s) 603, 617, 691, 975
TseI GCWGC 5 cut(s) 95, 215, 476, 515, 574
TspDTI ATGAA 2 cut(s) 451, 1105
TspRI CASTG 4 cut(s) 603, 617, 691, 975
Van91I CCANNNNNTGG 1 cut(s) 400
VpaK11BI GGWCC 1 cut(s) 403
VspI ATTAAT 1 cut(s) 54
XceI RCATGY 1 cut(s) 1025
XspI CTAG 2 cut(s) 210, 771
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.