MD00G1003500.v1.1

alpha/beta hydrolase fold

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0000860
Physical Location & Seq
Forward (+)
555021 .. 555803
783 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1003500.v1.1.491

Sequence Viewer

Length: 783 bp
ATGTCCGGTATCGTTTCCAACCCGGATGGCACGTACACCCGTCGCATACAATTCCCAACCGTACCCGCCGAGCCCAACCCGAATTCTCCAACGACTCGGGTCCTCTCCAAAGACGTCCCTGTAAACCCGGAAACCCGAACCACGGCCCGACTATTCCTCCCCCGCGAAGCCCTCGATTTACCCACCTCTAAACTCCCTCTCGTTTTTTACTACCACGGCGGCGCGTTCATTTACCTCAGCGCCGCGTCATCCAGCATCCACGACTTTTGTTCCGACATGGCTCTCCAGCTCCGAGCTCTTGTCGTCTCCCTGGAGTACCGCCTAGCACCCGAGCACCGCCTCCCGGCAGCATACGACGATGCTGTGCATGCGTTGCACTGGATTGCCTCCACCAGAGAAGAGTGGGTCACTGAATTTACAGATCTGTCCAGTTGCTTTTTAATGGGAACTAGCGCTGGGGGGAACCTGGCTTACCACGCGGGGCTGCGCGCGTGTGAAGATATTGGCGATCGTCTCCAGCCTCTGCGAATCAAGGGGCTGATATTGCACCATCCGTTCTTTGGTGGGTCCGACAGAACAGGGTCAGAGCTGGCAATAGCAGATGACCCAATTTTGTCGCTACCCGGGTGCGATGGAGGTGGAGTTTGGCGTTGCCCGTCGGCACAGACCGTGACCATGTGTACTGCAATCCAACGGTGGGAAAAGAATCTGAAGATCGTTACGTTGGAATTCGGGAGATGGGTTGGCGGGTCTTGGTTACGGGTTGTTTCGGAGACCCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

261

Amino Acids

28.6

Weight (kDa)

6.06

Isoelectric Point (pI)

49.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BD-FAE PF20434 62 - 165 1.9e-12 BD-FAE
Abhydrolase_3 PF07859 68 - 211 2.2e-35 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 117
AccII CGCG 6 cut(s) 165, 224, 245, 479, 489, 491
AciI CCGC 8 cut(s) 66, 163, 219, 243, 319, 337, 479, 747
AcsI RAATTY 3 cut(s) 82, 413, 728
AcuI CTGAAG 1 cut(s) 731
AcyI GRCGYC 1 cut(s) 114
AfaI GTAC 4 cut(s) 35, 63, 317, 682
AfeI AGCGCT 1 cut(s) 454
AfiI CCNNNNNNNGG 4 cut(s) 142, 343, 560, 697
AjnI CCWGG 2 cut(s) 309, 465
AluBI AGCT 3 cut(s) 289, 296, 589
AluI AGCT 3 cut(s) 289, 296, 589
Alw21I GWGCWC 2 cut(s) 298, 336
Alw26I GTCTC 3 cut(s) 310, 518, 767
AlwNI CAGNNNCTG 1 cut(s) 523
Ama87I CYCGRG 3 cut(s) 96, 329, 623
Aor51HI AGCGCT 1 cut(s) 454
AoxI GGCC 1 cut(s) 144
ApeKI GCWGC 2 cut(s) 347, 484
ApoI RAATTY 3 cut(s) 82, 413, 728
AspLEI GCGC 5 cut(s) 224, 242, 455, 489, 491
AspS9I GGNCC 3 cut(s) 100, 145, 567
AsuC2I CCSGG 5 cut(s) 23, 128, 344, 624, 625
AvaI CYCGRG 3 cut(s) 96, 329, 623
AvaII GGWCC 2 cut(s) 100, 567
BanII GRGCYC 2 cut(s) 75, 298
Bbv12I GWGCWC 2 cut(s) 298, 336
BbvCI CCTCAGC 1 cut(s) 236
BbvI GCAGC 2 cut(s) 359, 471
BccI CCATC 4 cut(s) 20, 558, 626, 732
BceAI ACGGC 2 cut(s) 159, 232
BciT130I CCWGG 2 cut(s) 311, 467
BcnI CCSGG 5 cut(s) 23, 128, 344, 624, 625
BcoDI GTCTC 3 cut(s) 310, 518, 767
BfaI CTAG 2 cut(s) 323, 450
BfoI RGCGCY 2 cut(s) 243, 456
BglII AGATCT 1 cut(s) 421
BisI GCNGC 4 cut(s) 220, 243, 348, 485
BlsI GCNGC 4 cut(s) 221, 244, 349, 486
Bme1390I CCNGG 7 cut(s) 23, 128, 311, 344, 467, 624, 625
Bme18I GGWCC 2 cut(s) 100, 567
BmeT110I CYCGRG 3 cut(s) 96, 329, 623
BmgT120I GGNCC 3 cut(s) 100, 145, 567
BmiI GGNNCC 3 cut(s) 101, 464, 568
BmrFI CCNGG 7 cut(s) 23, 128, 311, 344, 467, 624, 625
BmsI GCATC 2 cut(s) 264, 349
BoxI GACNNNNGTC 1 cut(s) 98
BpmI CTGGAG 3 cut(s) 269, 332, 500
Bpu10I CCTNAGC 1 cut(s) 236
BpuMI CCSGG 5 cut(s) 23, 128, 344, 624, 625
BsaAI YACGTR 1 cut(s) 33
BsaHI GRCGYC 1 cut(s) 114
BsaI GGTCTC 1 cut(s) 767
BsaJI CCNNGG 4 cut(s) 141, 214, 309, 623
BsaWI WCCGGW 1 cut(s) 5
BsaXI ACNNNNNCTCC 4 cut(s) 141, 171, 627, 657
Bsc4I CCNNNNNNNGG 4 cut(s) 142, 343, 560, 697
Bse1I ACTGG 2 cut(s) 383, 429
BseBI CCWGG 2 cut(s) 311, 467
BseDI CCNNGG 4 cut(s) 141, 214, 309, 623
BseGI GGATG 4 cut(s) 31, 248, 255, 550
BseLI CCNNNNNNNGG 4 cut(s) 142, 343, 560, 697
BseMII CTCAG 1 cut(s) 250
BseNI ACTGG 2 cut(s) 383, 429
BsePI GCGCGC 1 cut(s) 487
BseXI GCAGC 2 cut(s) 359, 471
BseYI CCCAGC 1 cut(s) 455
Bsh1236I CGCG 6 cut(s) 165, 224, 245, 479, 489, 491
Bsh1285I CGRYCG 1 cut(s) 511
BshFI GGCC 1 cut(s) 146
BsiEI CGRYCG 1 cut(s) 511
BsiHKAI GWGCWC 2 cut(s) 298, 336
BsiHKCI CYCGRG 3 cut(s) 96, 329, 623
BsiSI CCGG 5 cut(s) 6, 23, 128, 344, 624
BslFI GGGAC 1 cut(s) 101
BslI CCNNNNNNNGG 4 cut(s) 142, 343, 560, 697
BsmAI GTCTC 3 cut(s) 310, 518, 767
BsmBI CGTCTC 2 cut(s) 310, 518
BsmFI GGGAC 1 cut(s) 101
BsnI GGCC 1 cut(s) 146
Bso31I GGTCTC 1 cut(s) 767
BsoBI CYCGRG 3 cut(s) 96, 329, 623
Bsp1286I GDGCHC 3 cut(s) 75, 298, 336
Bsp143I GATC 3 cut(s) 421, 508, 714
BspACI CCGC 8 cut(s) 66, 163, 219, 243, 319, 337, 479, 747
BspANI GGCC 1 cut(s) 146
BspCNI CTCAG 1 cut(s) 249
BspFNI CGCG 6 cut(s) 165, 224, 245, 479, 489, 491
BspLI GGNNCC 3 cut(s) 101, 464, 568
BspTNI GGTCTC 1 cut(s) 767
BsrI ACTGG 2 cut(s) 383, 429
BssECI CCNNGG 4 cut(s) 141, 214, 309, 623
BssHII GCGCGC 1 cut(s) 487
BssMI GATC 3 cut(s) 421, 508, 714
BssNI GRCGYC 1 cut(s) 114
Bst2UI CCWGG 2 cut(s) 311, 467
Bst4CI ACNGT 3 cut(s) 61, 670, 696
Bst6I CTCTTC 1 cut(s) 393
BstACI GRCGYC 1 cut(s) 114
BstAPI GCANNNNNTGC 1 cut(s) 373
BstBAI YACGTR 1 cut(s) 33
BstC8I GCNNGC 3 cut(s) 369, 489, 591
BstDEI CTNAG 1 cut(s) 236
BstDSI CCRYGG 2 cut(s) 141, 214
BstF5I GGATG 4 cut(s) 31, 248, 255, 550
BstFNI CGCG 6 cut(s) 165, 224, 245, 479, 489, 491
BstH2I RGCGCY 2 cut(s) 243, 456
BstHHI GCGC 5 cut(s) 224, 242, 455, 489, 491
BstKTI GATC 3 cut(s) 424, 511, 717
BstMAI GTCTC 3 cut(s) 310, 518, 767
BstMBI GATC 3 cut(s) 421, 508, 714
BstMCI CGRYCG 1 cut(s) 511
BstMWI GCNNNNNNNGC 4 cut(s) 368, 373, 476, 544
BstNI CCWGG 2 cut(s) 311, 467
BstNSI RCATGY 1 cut(s) 371
BstPAI GACNNNNGTC 1 cut(s) 98
BstSCI CCNGG 7 cut(s) 21, 126, 309, 342, 465, 622, 623
BstUI CGCG 6 cut(s) 165, 224, 245, 479, 489, 491
BstV1I GCAGC 2 cut(s) 359, 471
BstX2I RGATCY 1 cut(s) 421
BstYI RGATCY 1 cut(s) 421
BsuRI GGCC 1 cut(s) 146
BtgI CCRYGG 2 cut(s) 141, 214
BtgZI GCGATG 1 cut(s) 645
BtsCI GGATG 4 cut(s) 31, 248, 255, 550
BtsIMutI CAGTG 2 cut(s) 376, 408
Cac8I GCNNGC 3 cut(s) 369, 489, 591
CaiI CAGNNNCTG 1 cut(s) 523
CfoI GCGC 5 cut(s) 224, 242, 455, 489, 491
Cfr13I GGNCC 3 cut(s) 100, 145, 567
Cfr9I CCCGGG 1 cut(s) 623
CseI GACGC 1 cut(s) 234
Csp6I GTAC 4 cut(s) 34, 62, 316, 681
CspCI CAANNNNNGTGG 2 cut(s) 248, 283
CviAII CATG 3 cut(s) 277, 368, 676
CviQI GTAC 4 cut(s) 34, 62, 316, 681
DdeI CTNAG 1 cut(s) 236
DpnI GATC 3 cut(s) 423, 510, 716
DpnII GATC 3 cut(s) 421, 508, 714
Eam1104I CTCTTC 1 cut(s) 393
EarI CTCTTC 1 cut(s) 393
Ecl136II GAGCTC 1 cut(s) 296
Eco24I GRGCYC 2 cut(s) 75, 298
Eco31I GGTCTC 1 cut(s) 767
Eco47I GGWCC 2 cut(s) 100, 567
Eco47III AGCGCT 1 cut(s) 454
Eco53kI GAGCTC 1 cut(s) 296
Eco57I CTGAAG 1 cut(s) 731
Eco88I CYCGRG 3 cut(s) 96, 329, 623
EcoICRI GAGCTC 1 cut(s) 296
EcoO109I RGGNCCY 1 cut(s) 100
EcoRI GAATTC 2 cut(s) 82, 728
EcoRII CCWGG 2 cut(s) 309, 465
EcoT38I GRGCYC 2 cut(s) 75, 298
Esp3I CGTCTC 2 cut(s) 310, 518
FaeI CATG 3 cut(s) 280, 371, 679
FaiI YATR 5 cut(s) 47, 278, 352, 369, 677
FaqI GGGAC 1 cut(s) 101
FatI CATG 3 cut(s) 276, 367, 675
FauI CCCGC 4 cut(s) 73, 170, 472, 740
Fnu4HI GCNGC 4 cut(s) 220, 243, 348, 485
FokI GGATG 4 cut(s) 38, 235, 242, 537
FriOI GRGCYC 2 cut(s) 75, 298
Fsp4HI GCNGC 4 cut(s) 220, 243, 348, 485
FspBI CTAG 2 cut(s) 323, 450
GlaI GCGC 5 cut(s) 223, 241, 454, 488, 490
GluI GCNGC 4 cut(s) 220, 243, 348, 485
GsaI CCCAGC 1 cut(s) 459
GsuI CTGGAG 3 cut(s) 269, 332, 500
HaeII RGCGCY 2 cut(s) 243, 456
HaeIII GGCC 1 cut(s) 146
HapII CCGG 5 cut(s) 6, 23, 128, 344, 624
HgaI GACGC 1 cut(s) 234
HhaI GCGC 5 cut(s) 224, 242, 455, 489, 491
Hin1I GRCGYC 1 cut(s) 114
Hin1II CATG 3 cut(s) 280, 371, 679
Hin6I GCGC 5 cut(s) 222, 240, 453, 487, 489
HinP1I GCGC 5 cut(s) 222, 240, 453, 487, 489
HinfI GANTC 3 cut(s) 94, 528, 706
HpaII CCGG 5 cut(s) 6, 23, 128, 344, 624
Hpy166II GTNNAC 3 cut(s) 36, 124, 681
Hpy188I TCNGA 6 cut(s) 274, 293, 571, 586, 711, 772
Hpy188III TCNNGA 1 cut(s) 733
Hpy8I GTNNAC 3 cut(s) 36, 124, 681
Hpy99I CGWCG 3 cut(s) 45, 359, 661
HpyCH4III ACNGT 3 cut(s) 61, 670, 696
HpyCH4IV ACGT 3 cut(s) 32, 114, 722
HpyCH4V TGCA 4 cut(s) 367, 376, 547, 686
HpyF10VI GCNNNNNNNGC 4 cut(s) 368, 373, 476, 544
HpyF3I CTNAG 1 cut(s) 236
HpySE526I ACGT 3 cut(s) 32, 114, 722
Hsp92I GRCGYC 1 cut(s) 114
Hsp92II CATG 3 cut(s) 280, 371, 679
HspAI GCGC 5 cut(s) 222, 240, 453, 487, 489
Kzo9I GATC 3 cut(s) 421, 508, 714
LmnI GCTCC 1 cut(s) 294
Lsp1109I GCAGC 2 cut(s) 359, 471
LweI GCATC 2 cut(s) 264, 349
MaeI CTAG 2 cut(s) 323, 450
MaeII ACGT 3 cut(s) 32, 114, 722
MaeIII GTNAC 4 cut(s) 406, 670, 718, 756
MalI GATC 3 cut(s) 423, 510, 716
MboI GATC 3 cut(s) 421, 508, 714
MboII GAAGA 3 cut(s) 410, 509, 724
MflI RGATCY 1 cut(s) 421
MhlI GDGCHC 3 cut(s) 75, 298, 336
MluCI AATT 5 cut(s) 50, 82, 413, 609, 728
MlyI GAGTC 1 cut(s) 88
MmeI TCCRAC 6 cut(s) 42, 113, 297, 594, 705, 715
MseI TTAA 1 cut(s) 440
MspI CCGG 5 cut(s) 6, 23, 128, 344, 624
MspR9I CCNGG 7 cut(s) 23, 128, 311, 344, 467, 624, 625
MvaI CCWGG 2 cut(s) 311, 467
MvnI CGCG 6 cut(s) 165, 224, 245, 479, 489, 491
MwoI GCNNNNNNNGC 4 cut(s) 368, 373, 476, 544
NciI CCSGG 5 cut(s) 23, 128, 344, 624, 625
NdeII GATC 3 cut(s) 421, 508, 714
NlaIII CATG 3 cut(s) 280, 371, 679
NlaIV GGNNCC 3 cut(s) 101, 464, 568
NmeAIII GCCGAG 1 cut(s) 94
NmuCI GTSAC 2 cut(s) 406, 670
NspI RCATGY 1 cut(s) 371
PaeI GCATGC 1 cut(s) 371
PauI GCGCGC 1 cut(s) 487
PfeI GAWTC 2 cut(s) 528, 706
PkrI GCNGC 4 cut(s) 221, 244, 349, 486
Ple19I CGATCG 1 cut(s) 511
PleI GAGTC 1 cut(s) 88
PpsI GAGTC 1 cut(s) 88
Ppu21I YACGTR 1 cut(s) 33
PpuMI RGGWCCY 1 cut(s) 100
PshAI GACNNNNGTC 1 cut(s) 98
Psp124BI GAGCTC 1 cut(s) 298
Psp5II RGGWCCY 1 cut(s) 100
Psp6I CCWGG 2 cut(s) 309, 465
PspFI CCCAGC 1 cut(s) 455
PspGI CCWGG 2 cut(s) 309, 465
PspN4I GGNNCC 3 cut(s) 101, 464, 568
PspPI GGNCC 3 cut(s) 100, 145, 567
PspPPI RGGWCCY 1 cut(s) 100
PsrI GAACNNNNNNTAC 2 cut(s) 455, 487
PstNI CAGNNNCTG 1 cut(s) 523
PsuI RGATCY 1 cut(s) 421
PteI GCGCGC 1 cut(s) 487
PvuI CGATCG 1 cut(s) 511
RsaI GTAC 4 cut(s) 35, 63, 317, 682
RsaNI GTAC 4 cut(s) 34, 62, 316, 681
SacI GAGCTC 1 cut(s) 298
SaqAI TTAA 1 cut(s) 440
SatI GCNGC 4 cut(s) 220, 243, 348, 485
Sau3AI GATC 3 cut(s) 421, 508, 714
Sau96I GGNCC 3 cut(s) 100, 145, 567
SchI GAGTC 1 cut(s) 88
ScrFI CCNGG 7 cut(s) 23, 128, 311, 344, 467, 624, 625
SduI GDGCHC 3 cut(s) 75, 298, 336
SfaNI GCATC 2 cut(s) 264, 349
SinI GGWCC 2 cut(s) 100, 567
SmaI CCCGGG 1 cut(s) 625
SphI GCATGC 1 cut(s) 371
Sse9I AATT 5 cut(s) 50, 82, 413, 609, 728
SsiI CCGC 8 cut(s) 66, 163, 219, 243, 319, 337, 479, 747
SspMI CTAG 2 cut(s) 323, 450
SstI GAGCTC 1 cut(s) 298
StyD4I CCNGG 7 cut(s) 21, 126, 309, 342, 465, 622, 623
TaaI ACNGT 3 cut(s) 61, 670, 696
TaiI ACGT 3 cut(s) 35, 117, 725
TaqI TCGA 1 cut(s) 174
TasI AATT 5 cut(s) 50, 82, 413, 609, 728
TatI WGTACW 1 cut(s) 680
TauI GCSGC 2 cut(s) 222, 245
TfiI GAWTC 2 cut(s) 528, 706
Tru1I TTAA 1 cut(s) 440
Tru9I TTAA 1 cut(s) 440
TscAI CASTG 2 cut(s) 383, 415
TseFI GTSAC 2 cut(s) 406, 670
TseI GCWGC 2 cut(s) 347, 484
Tsp45I GTSAC 2 cut(s) 406, 670
TspDTI ATGAA 1 cut(s) 217
TspGWI ACGGA 1 cut(s) 543
TspMI CCCGGG 1 cut(s) 623
TspRI CASTG 2 cut(s) 383, 415
VpaK11BI GGWCC 2 cut(s) 100, 567
XapI RAATTY 3 cut(s) 82, 413, 728
XceI RCATGY 1 cut(s) 371
XcmI CCANNNNNNNNNTGG 1 cut(s) 557
XmaI CCCGGG 1 cut(s) 623
XspI CTAG 2 cut(s) 323, 450
ZraI GACGTC 1 cut(s) 115
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.