pycom07g23900

alpha/beta hydrolase fold

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0000860
Physical Location & Seq
Forward (+)
24679819 .. 24680602
784 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g23900.2

Sequence Viewer

Length: 702 bp
ATGTCCGGTATCATTTCCAACCCGGATGGCACGTACACCCGTCGCTTACAATTCCCAACCGTACCCGCCGAGCCCAACCCGACTTCTCCAACGACTCGGATCCTCTCCAAAGACGTCCCTGTAAACCCGGAAACGCGAACCACGGCCCGACTATTCCTCCCCCGCGAAGCCCTCGATTTACCCACCTCTAAACTTCCTCTCGTTTTTTACTACCACGGCGGCGCGTTCGTTTACCTCAGTGCCGCGTCATCCAGCAACCACGACTTTTGTTCCGACATGGCTCTCCAGCTCCGAGCTCTTGTCGTCTCCCTGGAGTACCGCCTAGCACCCGAGCACCGCCTCCCGGCGGCATACGATGATGCTGTGGATGCGTTGCACTGGATTGCCTCCACCAAAGAAGAGTGGGTCACTGAATTTACAGATCTGTCCAGTTGCTTTTTAATGGGAACCAGCGCTGGGGGGAACCTGGCTTACCACGCGGGGCTGCGCGCGTGTGAAGGTCAGAGCTGGCGATGGCTGATGACCCGGTTTTGTCGCTACCCGGGTGCGATGGAGCGTGGAGTTTGGCGTTGCCCGTCGGCACAGACCGTGACCATGAGTACTGTAATCCAACGGTGGCAAAAGAATCTGATGATCGTTACGTTGGAATTCGGGAGATGGGTTGGCGGGTCTTGGTTACGGGTTGTTTCGGAGACCCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

234

Amino Acids

26.15

Weight (kDa)

7.67

Isoelectric Point (pI)

51.86

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 117
AccII CGCG 7 cut(s) 136, 165, 224, 245, 479, 489, 491
AciI CCGC 9 cut(s) 66, 163, 219, 243, 319, 337, 347, 479, 666
AclWI GGATC 2 cut(s) 94, 107
AcsI RAATTY 2 cut(s) 413, 647
AcyI GRCGYC 1 cut(s) 114
AfaI GTAC 4 cut(s) 35, 63, 317, 601
AfeI AGCGCT 1 cut(s) 454
AfiI CCNNNNNNNGG 3 cut(s) 343, 346, 456
AjnI CCWGG 2 cut(s) 309, 465
AluBI AGCT 3 cut(s) 289, 296, 507
AluI AGCT 3 cut(s) 289, 296, 507
Alw21I GWGCWC 2 cut(s) 298, 336
Alw26I GTCTC 2 cut(s) 310, 686
AlwI GGATC 2 cut(s) 94, 107
Ama87I CYCGRG 2 cut(s) 329, 541
Aor51HI AGCGCT 1 cut(s) 454
AoxI GGCC 1 cut(s) 144
ApeKI GCWGC 1 cut(s) 484
ApoI RAATTY 2 cut(s) 413, 647
AspLEI GCGC 4 cut(s) 224, 455, 489, 491
AspS9I GGNCC 1 cut(s) 145
AsuC2I CCSGG 6 cut(s) 23, 128, 344, 526, 542, 543
AvaI CYCGRG 2 cut(s) 329, 541
BamHI GGATCC 1 cut(s) 99
BanII GRGCYC 2 cut(s) 75, 298
Bbv12I GWGCWC 2 cut(s) 298, 336
BbvI GCAGC 1 cut(s) 471
BccI CCATC 4 cut(s) 20, 507, 544, 651
BceAI ACGGC 2 cut(s) 159, 232
BciT130I CCWGG 2 cut(s) 311, 467
BcnI CCSGG 6 cut(s) 23, 128, 344, 526, 542, 543
BcoDI GTCTC 2 cut(s) 310, 686
BfaI CTAG 1 cut(s) 323
BfoI RGCGCY 1 cut(s) 456
BglII AGATCT 1 cut(s) 421
BisI GCNGC 4 cut(s) 220, 243, 348, 485
BlsI GCNGC 4 cut(s) 221, 244, 349, 486
BmcAI AGTACT 1 cut(s) 601
Bme1390I CCNGG 8 cut(s) 23, 128, 311, 344, 467, 526, 542, 543
BmeT110I CYCGRG 2 cut(s) 329, 541
BmgT120I GGNCC 1 cut(s) 145
BmiI GGNNCC 3 cut(s) 101, 448, 464
BmrFI CCNGG 8 cut(s) 23, 128, 311, 344, 467, 526, 542, 543
BmsI GCATC 2 cut(s) 349, 358
BpmI CTGGAG 2 cut(s) 269, 332
BpuMI CCSGG 6 cut(s) 23, 128, 344, 526, 542, 543
BsaAI YACGTR 1 cut(s) 33
BsaHI GRCGYC 1 cut(s) 114
BsaI GGTCTC 1 cut(s) 686
BsaJI CCNNGG 4 cut(s) 141, 214, 309, 541
BsaWI WCCGGW 1 cut(s) 5
BsaXI ACNNNNNCTCC 2 cut(s) 141, 171
Bsc4I CCNNNNNNNGG 3 cut(s) 343, 346, 456
Bse1I ACTGG 2 cut(s) 383, 429
BseBI CCWGG 2 cut(s) 311, 467
BseDI CCNNGG 4 cut(s) 141, 214, 309, 541
BseGI GGATG 3 cut(s) 31, 248, 373
BseLI CCNNNNNNNGG 3 cut(s) 343, 346, 456
BseMII CTCAG 1 cut(s) 250
BseNI ACTGG 2 cut(s) 383, 429
BsePI GCGCGC 1 cut(s) 487
BseXI GCAGC 1 cut(s) 471
BseYI CCCAGC 1 cut(s) 455
Bsh1236I CGCG 7 cut(s) 136, 165, 224, 245, 479, 489, 491
BshFI GGCC 1 cut(s) 146
BsiHKAI GWGCWC 2 cut(s) 298, 336
BsiHKCI CYCGRG 2 cut(s) 329, 541
BsiSI CCGG 6 cut(s) 6, 23, 128, 344, 526, 542
BslFI GGGAC 1 cut(s) 101
BslI CCNNNNNNNGG 3 cut(s) 343, 346, 456
BsmAI GTCTC 2 cut(s) 310, 686
BsmBI CGTCTC 1 cut(s) 310
BsmFI GGGAC 1 cut(s) 101
BsnI GGCC 1 cut(s) 146
Bso31I GGTCTC 1 cut(s) 686
BsoBI CYCGRG 2 cut(s) 329, 541
Bsp1286I GDGCHC 3 cut(s) 75, 298, 336
Bsp143I GATC 3 cut(s) 99, 421, 633
BspACI CCGC 9 cut(s) 66, 163, 219, 243, 319, 337, 347, 479, 666
BspANI GGCC 1 cut(s) 146
BspCNI CTCAG 1 cut(s) 249
BspFNI CGCG 7 cut(s) 136, 165, 224, 245, 479, 489, 491
BspLI GGNNCC 3 cut(s) 101, 448, 464
BspPI GGATC 2 cut(s) 94, 107
BspTNI GGTCTC 1 cut(s) 686
BsrI ACTGG 2 cut(s) 383, 429
BssECI CCNNGG 4 cut(s) 141, 214, 309, 541
BssHII GCGCGC 1 cut(s) 487
BssMI GATC 3 cut(s) 99, 421, 633
BssNI GRCGYC 1 cut(s) 114
Bst2UI CCWGG 2 cut(s) 311, 467
Bst4CI ACNGT 4 cut(s) 61, 589, 604, 615
Bst6I CTCTTC 1 cut(s) 393
BstACI GRCGYC 1 cut(s) 114
BstBAI YACGTR 1 cut(s) 33
BstC8I GCNNGC 2 cut(s) 489, 509
BstDEI CTNAG 1 cut(s) 236
BstDSI CCRYGG 2 cut(s) 141, 214
BstF5I GGATG 3 cut(s) 31, 248, 373
BstFNI CGCG 7 cut(s) 136, 165, 224, 245, 479, 489, 491
BstH2I RGCGCY 1 cut(s) 456
BstHHI GCGC 4 cut(s) 224, 455, 489, 491
BstKTI GATC 3 cut(s) 102, 424, 636
BstMAI GTCTC 2 cut(s) 310, 686
BstMBI GATC 3 cut(s) 99, 421, 633
BstMWI GCNNNNNNNGC 2 cut(s) 368, 476
BstNI CCWGG 2 cut(s) 311, 467
BstSCI CCNGG 8 cut(s) 21, 126, 309, 342, 465, 524, 540, 541
BstUI CGCG 7 cut(s) 136, 165, 224, 245, 479, 489, 491
BstV1I GCAGC 1 cut(s) 471
BstX2I RGATCY 2 cut(s) 99, 421
BstYI RGATCY 2 cut(s) 99, 421
BsuRI GGCC 1 cut(s) 146
BtgI CCRYGG 2 cut(s) 141, 214
BtgZI GCGATG 2 cut(s) 526, 563
BtsCI GGATG 3 cut(s) 31, 248, 373
BtsIMutI CAGTG 3 cut(s) 244, 376, 408
Cac8I GCNNGC 2 cut(s) 489, 509
CfoI GCGC 4 cut(s) 224, 455, 489, 491
Cfr13I GGNCC 1 cut(s) 145
Cfr9I CCCGGG 1 cut(s) 541
CseI GACGC 1 cut(s) 234
Csp6I GTAC 4 cut(s) 34, 62, 316, 600
CspCI CAANNNNNGTGG 2 cut(s) 248, 283
CviAII CATG 2 cut(s) 277, 595
CviQI GTAC 4 cut(s) 34, 62, 316, 600
DdeI CTNAG 1 cut(s) 236
DpnI GATC 3 cut(s) 101, 423, 635
DpnII GATC 3 cut(s) 99, 421, 633
Eam1104I CTCTTC 1 cut(s) 393
EarI CTCTTC 1 cut(s) 393
Ecl136II GAGCTC 1 cut(s) 296
Eco24I GRGCYC 2 cut(s) 75, 298
Eco31I GGTCTC 1 cut(s) 686
Eco47III AGCGCT 1 cut(s) 454
Eco53kI GAGCTC 1 cut(s) 296
Eco88I CYCGRG 2 cut(s) 329, 541
EcoICRI GAGCTC 1 cut(s) 296
EcoRI GAATTC 1 cut(s) 647
EcoRII CCWGG 2 cut(s) 309, 465
EcoT38I GRGCYC 2 cut(s) 75, 298
Esp3I CGTCTC 1 cut(s) 310
FaeI CATG 2 cut(s) 280, 598
FaiI YATR 3 cut(s) 278, 352, 596
FaqI GGGAC 1 cut(s) 101
FatI CATG 2 cut(s) 276, 594
FauI CCCGC 4 cut(s) 73, 170, 472, 659
Fnu4HI GCNGC 4 cut(s) 220, 243, 348, 485
FokI GGATG 3 cut(s) 38, 235, 380
FriOI GRGCYC 2 cut(s) 75, 298
Fsp4HI GCNGC 4 cut(s) 220, 243, 348, 485
FspBI CTAG 1 cut(s) 323
GlaI GCGC 4 cut(s) 223, 454, 488, 490
GluI GCNGC 4 cut(s) 220, 243, 348, 485
GsaI CCCAGC 1 cut(s) 459
GsuI CTGGAG 2 cut(s) 269, 332
HaeII RGCGCY 1 cut(s) 456
HaeIII GGCC 1 cut(s) 146
HapII CCGG 6 cut(s) 6, 23, 128, 344, 526, 542
HgaI GACGC 1 cut(s) 234
HhaI GCGC 4 cut(s) 224, 455, 489, 491
Hin1I GRCGYC 1 cut(s) 114
Hin1II CATG 2 cut(s) 280, 598
Hin6I GCGC 4 cut(s) 222, 453, 487, 489
HinP1I GCGC 4 cut(s) 222, 453, 487, 489
HinfI GANTC 2 cut(s) 94, 625
HpaII CCGG 6 cut(s) 6, 23, 128, 344, 526, 542
Hpy166II GTNNAC 3 cut(s) 36, 124, 232
Hpy188I TCNGA 6 cut(s) 99, 274, 293, 504, 630, 691
Hpy188III TCNNGA 1 cut(s) 652
Hpy8I GTNNAC 3 cut(s) 36, 124, 232
Hpy99I CGWCG 2 cut(s) 45, 580
HpyAV CCTTC 1 cut(s) 491
HpyCH4III ACNGT 4 cut(s) 61, 589, 604, 615
HpyCH4IV ACGT 3 cut(s) 32, 114, 641
HpyCH4V TGCA 1 cut(s) 376
HpyF10VI GCNNNNNNNGC 2 cut(s) 368, 476
HpyF3I CTNAG 1 cut(s) 236
HpySE526I ACGT 3 cut(s) 32, 114, 641
Hsp92I GRCGYC 1 cut(s) 114
Hsp92II CATG 2 cut(s) 280, 598
HspAI GCGC 4 cut(s) 222, 453, 487, 489
Kzo9I GATC 3 cut(s) 99, 421, 633
LmnI GCTCC 2 cut(s) 294, 553
Lsp1109I GCAGC 1 cut(s) 471
LweI GCATC 2 cut(s) 349, 358
MaeI CTAG 1 cut(s) 323
MaeII ACGT 3 cut(s) 32, 114, 641
MaeIII GTNAC 4 cut(s) 406, 589, 637, 675
MalI GATC 3 cut(s) 101, 423, 635
MboI GATC 3 cut(s) 99, 421, 633
MboII GAAGA 1 cut(s) 410
MflI RGATCY 2 cut(s) 99, 421
MhlI GDGCHC 3 cut(s) 75, 298, 336
MluCI AATT 3 cut(s) 50, 413, 647
MlyI GAGTC 1 cut(s) 88
MmeI TCCRAC 5 cut(s) 42, 113, 297, 624, 634
MnlI CCTC 8 cut(s) 113, 167, 182, 196, 207, 245, 350, 397
MseI TTAA 1 cut(s) 440
MspI CCGG 6 cut(s) 6, 23, 128, 344, 526, 542
MspR9I CCNGG 8 cut(s) 23, 128, 311, 344, 467, 526, 542, 543
MvaI CCWGG 2 cut(s) 311, 467
MvnI CGCG 7 cut(s) 136, 165, 224, 245, 479, 489, 491
MwoI GCNNNNNNNGC 2 cut(s) 368, 476
NciI CCSGG 6 cut(s) 23, 128, 344, 526, 542, 543
NdeII GATC 3 cut(s) 99, 421, 633
NlaIII CATG 2 cut(s) 280, 598
NlaIV GGNNCC 3 cut(s) 101, 448, 464
NmeAIII GCCGAG 1 cut(s) 94
NmuCI GTSAC 2 cut(s) 406, 589
PauI GCGCGC 1 cut(s) 487
PfeI GAWTC 1 cut(s) 625
PkrI GCNGC 4 cut(s) 221, 244, 349, 486
PleI GAGTC 1 cut(s) 88
PpsI GAGTC 1 cut(s) 88
Ppu21I YACGTR 1 cut(s) 33
Psp124BI GAGCTC 1 cut(s) 298
Psp6I CCWGG 2 cut(s) 309, 465
PspFI CCCAGC 1 cut(s) 455
PspGI CCWGG 2 cut(s) 309, 465
PspN4I GGNNCC 3 cut(s) 101, 448, 464
PspPI GGNCC 1 cut(s) 145
PsrI GAACNNNNNNTAC 2 cut(s) 455, 487
PsuI RGATCY 2 cut(s) 99, 421
PteI GCGCGC 1 cut(s) 487
RsaI GTAC 4 cut(s) 35, 63, 317, 601
RsaNI GTAC 4 cut(s) 34, 62, 316, 600
SacI GAGCTC 1 cut(s) 298
SaqAI TTAA 1 cut(s) 440
SatI GCNGC 4 cut(s) 220, 243, 348, 485
Sau3AI GATC 3 cut(s) 99, 421, 633
Sau96I GGNCC 1 cut(s) 145
ScaI AGTACT 1 cut(s) 601
SchI GAGTC 1 cut(s) 88
ScrFI CCNGG 8 cut(s) 23, 128, 311, 344, 467, 526, 542, 543
SduI GDGCHC 3 cut(s) 75, 298, 336
SfaNI GCATC 2 cut(s) 349, 358
SmaI CCCGGG 1 cut(s) 543
Sse9I AATT 3 cut(s) 50, 413, 647
SsiI CCGC 9 cut(s) 66, 163, 219, 243, 319, 337, 347, 479, 666
SspMI CTAG 1 cut(s) 323
SstI GAGCTC 1 cut(s) 298
StyD4I CCNGG 8 cut(s) 21, 126, 309, 342, 465, 524, 540, 541
TaaI ACNGT 4 cut(s) 61, 589, 604, 615
TaiI ACGT 3 cut(s) 35, 117, 644
TaqI TCGA 1 cut(s) 174
TasI AATT 3 cut(s) 50, 413, 647
TatI WGTACW 1 cut(s) 599
TauI GCSGC 3 cut(s) 222, 245, 350
TfiI GAWTC 1 cut(s) 625
Tru1I TTAA 1 cut(s) 440
Tru9I TTAA 1 cut(s) 440
TscAI CASTG 3 cut(s) 244, 383, 415
TseFI GTSAC 2 cut(s) 406, 589
TseI GCWGC 1 cut(s) 484
Tsp45I GTSAC 2 cut(s) 406, 589
TspMI CCCGGG 1 cut(s) 541
TspRI CASTG 3 cut(s) 244, 383, 415
XapI RAATTY 2 cut(s) 413, 647
XmaI CCCGGG 1 cut(s) 541
XspI CTAG 1 cut(s) 323
ZraI GACGTC 1 cut(s) 115
ZrmI AGTACT 1 cut(s) 601
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.