Rroxscaffold_4G00281930

Carboxylesterase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000860
Physical Location & Seq
Forward (+)
4133945 .. 4134942
998 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00281930.1

Sequence Viewer

Length: 867 bp
ATGTCAAATGAAATGGCAGCAGCGGATTCCGATCCCAACTCCGGCGTTGATCCTTACCAGCGCCTCCAGATAGCACGCAATCCCGTCGGCAGCATCACGCGCTTGTTCAAATGTCCAGACGCCCCGCCTTCATCTGATCCCAAACTTTCCACTCCAGTTCTCACCAAGGACATCCCCATCAACCAATCAAATAATACCTGGCTTCGACTGTTCCTCCCCCGCCATGTACTGGACCAAACCTCACCAACTACCAAGCTCCCTCTCGTGGTTTATTACCATGGTGGAGGCTTCATATTACTTAGCGCAGCCTCAACCATTTTCCATGATTTCTGTTTCAACTTTGCAGCCGGCGTCCCCGTGATCGTCGCATCTGTCGAGTACAGTGCTGGGGGTAACATAGCCTACAATGCGGGACTTCTTGCGGCTGCTGAGGGCATTGACCTTCTCCACCCGTTGAAGATTAGAGGGCTGATATTGGTGCAACCGTATTTCGGGGGGAATCGAAGGACTAGTTCAGAGTTGAGGTTGGTGAACAACCCTGTTTTGGCGCCAAGTGTCTGTGATCTCATGTGGGACTTGTCGTTGCCTATCGGGGCTGACCGTGATCACGAGTATTGCAATCCAATGGCGGGTGGCGAGTCTAATTGCTTTGATCAAGTGAAAGCACTTGGGTGGAAAGTTTTGGTGAGTGGCTCTGATGGAGACCCACTGATCGATCGTCAGATTGAGCTGGTGAAGATGTTGGAGGGGAAGGGTGTACACGTGGTGAGTCAGTTTATTGAAGGAGGTTATCATGGGGCTCAAGATGTTGAACCCTCCAAAGCCAAGGATGTGTTTGTGCTTGTTAAGCATTTCATATTTCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0009056 GO:0016787
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

288

Amino Acids

31.24

Weight (kDa)

5.72

Isoelectric Point (pI)

38.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydrolase_3 PF07859 89 - 127 2.2e-06 alpha/beta hydrolase fold
Abhydrolase_3 PF07859 128 - 267 1e-17 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 547
AccB7I CCANNNNNTGG 1 cut(s) 229
AccII CGCG 1 cut(s) 100
AciI CCGC 6 cut(s) 23, 125, 220, 410, 422, 629
AclWI GGATC 3 cut(s) 26, 44, 131
AcvI CACGTG 1 cut(s) 763
AcyI GRCGYC 3 cut(s) 120, 351, 548
AdeI CACNNNGTG 1 cut(s) 766
AfaI GTAC 3 cut(s) 228, 380, 759
AfiI CCNNNNNNNGG 6 cut(s) 41, 229, 265, 491, 544, 629
AflIII ACRYGT 1 cut(s) 760
AgsI TTSAA 5 cut(s) 109, 337, 457, 782, 812
AhlI ACTAGT 1 cut(s) 509
AjnI CCWGG 1 cut(s) 197
AleI CACNNNNGTG 1 cut(s) 670
AluBI AGCT 2 cut(s) 256, 730
AluI AGCT 2 cut(s) 256, 730
Alw26I GTCTC 1 cut(s) 696
AlwI GGATC 3 cut(s) 26, 44, 131
ApeKI GCWGC 6 cut(s) 17, 20, 90, 305, 344, 425
AspLEI GCGC 4 cut(s) 63, 102, 305, 550
AspS9I GGNCC 1 cut(s) 232
AsuHPI GGTGA 6 cut(s) 154, 234, 541, 697, 745, 778
AvaII GGWCC 1 cut(s) 232
BanI GGYRCC 1 cut(s) 547
BanII GRGCYC 1 cut(s) 802
BauI CACGAG 2 cut(s) 263, 608
BbrPI CACGTG 1 cut(s) 763
BbvCI CCTCAGC 1 cut(s) 429
BbvI GCAGC 6 cut(s) 29, 32, 102, 317, 356, 412
BccI CCATC 2 cut(s) 185, 692
BcgI CGANNNNNNTGC 4 cut(s) 67, 101, 365, 399
BciT130I CCWGG 1 cut(s) 199
BclI TGATCA 2 cut(s) 604, 652
BcoDI GTCTC 1 cut(s) 696
BcuI ACTAGT 1 cut(s) 509
BfaI CTAG 1 cut(s) 510
BfoI RGCGCY 2 cut(s) 64, 551
BisI GCNGC 7 cut(s) 18, 21, 91, 306, 345, 423, 426
BlsI GCNGC 7 cut(s) 19, 22, 92, 307, 346, 424, 427
Bme1390I CCNGG 1 cut(s) 199
Bme18I GGWCC 1 cut(s) 232
BmgT120I GGNCC 1 cut(s) 232
BmiI GGNNCC 1 cut(s) 549
BmrFI CCNGG 1 cut(s) 199
BmsI GCATC 2 cut(s) 102, 377
BpmI CTGGAG 2 cut(s) 50, 138
Bpu10I CCTNAGC 1 cut(s) 429
BpuEI CTTGAG 1 cut(s) 786
Bsa29I ATCGAT 1 cut(s) 714
BsaAI YACGTR 1 cut(s) 763
BsaBI GATNNNNATC 1 cut(s) 30
BsaHI GRCGYC 3 cut(s) 120, 351, 548
BsaI GGTCTC 1 cut(s) 696
BsaJI CCNNGG 3 cut(s) 165, 277, 825
BsaXI ACNNNNNCTCC 2 cut(s) 198, 228
Bsc4I CCNNNNNNNGG 6 cut(s) 41, 229, 265, 491, 544, 629
Bse118I RCCGGY 1 cut(s) 347
Bse1I ACTGG 2 cut(s) 155, 234
Bse8I GATNNNNATC 1 cut(s) 30
BseBI CCWGG 1 cut(s) 199
BseCI ATCGAT 1 cut(s) 714
BseDI CCNNGG 3 cut(s) 165, 277, 825
BseGI GGATG 2 cut(s) 171, 835
BseJI GATNNNNATC 1 cut(s) 30
BseLI CCNNNNNNNGG 6 cut(s) 41, 229, 265, 491, 544, 629
BseMII CTCAG 1 cut(s) 420
BseNI ACTGG 2 cut(s) 155, 234
BseXI GCAGC 6 cut(s) 29, 32, 102, 317, 356, 412
BseYI CCCAGC 1 cut(s) 386
Bsh1236I CGCG 1 cut(s) 100
Bsh1285I CGRYCG 1 cut(s) 718
BshNI GGYRCC 1 cut(s) 547
BshVI ATCGAT 1 cut(s) 714
BsiEI CGRYCG 1 cut(s) 718
BsiSI CCGG 2 cut(s) 42, 348
BslFI GGGAC 3 cut(s) 338, 426, 587
BslI CCNNNNNNNGG 6 cut(s) 41, 229, 265, 491, 544, 629
BsmAI GTCTC 1 cut(s) 696
BsmFI GGGAC 3 cut(s) 338, 426, 587
Bso31I GGTCTC 1 cut(s) 696
Bsp1286I GDGCHC 1 cut(s) 802
Bsp1407I TGTACA 1 cut(s) 757
Bsp143I GATC 9 cut(s) 31, 49, 136, 360, 562, 604, 652, 711, 715
Bsp19I CCATGG 1 cut(s) 277
BspACI CCGC 6 cut(s) 23, 125, 220, 410, 422, 629
BspCNI CTCAG 1 cut(s) 421
BspDI ATCGAT 1 cut(s) 714
BspFNI CGCG 1 cut(s) 100
BspLI GGNNCC 1 cut(s) 549
BspPI GGATC 3 cut(s) 26, 44, 131
BspT107I GGYRCC 1 cut(s) 547
BspTNI GGTCTC 1 cut(s) 696
BsrFI RCCGGY 1 cut(s) 347
BsrGI TGTACA 1 cut(s) 757
BsrI ACTGG 2 cut(s) 155, 234
BssAI RCCGGY 1 cut(s) 347
BssECI CCNNGG 3 cut(s) 165, 277, 825
BssMI GATC 9 cut(s) 31, 49, 136, 360, 562, 604, 652, 711, 715
BssNI GRCGYC 3 cut(s) 120, 351, 548
BssSI CACGAG 2 cut(s) 263, 608
BssT1I CCWWGG 3 cut(s) 165, 277, 825
Bst2BI CACGAG 2 cut(s) 263, 608
Bst2UI CCWGG 1 cut(s) 199
Bst4CI ACNGT 4 cut(s) 210, 383, 486, 602
BstACI GRCGYC 3 cut(s) 120, 351, 548
BstAUI TGTACA 1 cut(s) 757
BstBAI YACGTR 1 cut(s) 763
BstC8I GCNNGC 2 cut(s) 76, 349
BstDEI CTNAG 2 cut(s) 299, 429
BstDSI CCRYGG 1 cut(s) 277
BstF5I GGATG 2 cut(s) 171, 835
BstFNI CGCG 1 cut(s) 100
BstH2I RGCGCY 2 cut(s) 64, 551
BstHHI GCGC 4 cut(s) 63, 102, 305, 550
BstKTI GATC 9 cut(s) 34, 52, 139, 363, 565, 607, 655, 714, 718
BstMAI GTCTC 1 cut(s) 696
BstMBI GATC 9 cut(s) 31, 49, 136, 360, 562, 604, 652, 711, 715
BstMCI CGRYCG 1 cut(s) 718
BstMWI GCNNNNNNNGC 3 cut(s) 99, 407, 847
BstNI CCWGG 1 cut(s) 199
BstSCI CCNGG 1 cut(s) 197
BstUI CGCG 1 cut(s) 100
BstV1I GCAGC 6 cut(s) 29, 32, 102, 317, 356, 412
Bsu15I ATCGAT 1 cut(s) 714
BsuTUI ATCGAT 1 cut(s) 714
BtgI CCRYGG 1 cut(s) 277
BtsCI GGATG 2 cut(s) 171, 835
BtsIMutI CAGTG 2 cut(s) 388, 707
Cac8I GCNNGC 2 cut(s) 76, 349
CfoI GCGC 4 cut(s) 63, 102, 305, 550
Cfr10I RCCGGY 1 cut(s) 347
Cfr13I GGNCC 1 cut(s) 232
ClaI ATCGAT 1 cut(s) 714
CseI GACGC 2 cut(s) 128, 340
Csp6I GTAC 3 cut(s) 227, 379, 758
CviAII CATG 5 cut(s) 224, 278, 323, 568, 794
CviQI GTAC 3 cut(s) 227, 379, 758
DdeI CTNAG 2 cut(s) 299, 429
DinI GGCGCC 1 cut(s) 549
DpnI GATC 9 cut(s) 33, 51, 138, 362, 564, 606, 654, 713, 717
DpnII GATC 9 cut(s) 31, 49, 136, 360, 562, 604, 652, 711, 715
DraIII CACNNNGTG 1 cut(s) 766
Eco130I CCWWGG 3 cut(s) 165, 277, 825
Eco24I GRGCYC 1 cut(s) 802
Eco31I GGTCTC 1 cut(s) 696
Eco47I GGWCC 1 cut(s) 232
Eco72I CACGTG 1 cut(s) 763
EcoRII CCWGG 1 cut(s) 197
EcoT14I CCWWGG 3 cut(s) 165, 277, 825
EcoT38I GRGCYC 1 cut(s) 802
EgeI GGCGCC 1 cut(s) 549
EheI GGCGCC 1 cut(s) 549
ErhI CCWWGG 3 cut(s) 165, 277, 825
FaeI CATG 5 cut(s) 227, 281, 326, 571, 797
FaiI YATR 8 cut(s) 225, 279, 293, 324, 398, 569, 795, 857
FaqI GGGAC 3 cut(s) 338, 426, 587
FatI CATG 5 cut(s) 223, 277, 322, 567, 793
FauI CCCGC 4 cut(s) 132, 227, 403, 622
FbaI TGATCA 2 cut(s) 604, 652
Fnu4HI GCNGC 7 cut(s) 18, 21, 91, 306, 345, 423, 426
FokI GGATG 2 cut(s) 158, 842
FriOI GRGCYC 1 cut(s) 802
Fsp4HI GCNGC 7 cut(s) 18, 21, 91, 306, 345, 423, 426
FspBI CTAG 1 cut(s) 510
GlaI GCGC 4 cut(s) 62, 101, 304, 549
GluI GCNGC 7 cut(s) 18, 21, 91, 306, 345, 423, 426
GsaI CCCAGC 1 cut(s) 390
GsuI CTGGAG 2 cut(s) 50, 138
HaeII RGCGCY 2 cut(s) 64, 551
HapII CCGG 2 cut(s) 42, 348
HgaI GACGC 2 cut(s) 128, 340
HhaI GCGC 4 cut(s) 63, 102, 305, 550
Hin1I GRCGYC 3 cut(s) 120, 351, 548
Hin1II CATG 5 cut(s) 227, 281, 326, 571, 797
Hin6I GCGC 4 cut(s) 61, 100, 303, 548
HinP1I GCGC 4 cut(s) 61, 100, 303, 548
HinfI GANTC 4 cut(s) 26, 499, 638, 769
HpaII CCGG 2 cut(s) 42, 348
HphI GGTGA 6 cut(s) 154, 234, 541, 697, 745, 778
Hpy166II GTNNAC 3 cut(s) 532, 758, 760
Hpy188I TCNGA 5 cut(s) 31, 136, 517, 697, 723
Hpy188III TCNNGA 4 cut(s) 67, 116, 608, 803
Hpy8I GTNNAC 3 cut(s) 532, 758, 760
Hpy99I CGWCG 2 cut(s) 89, 368
HpyAV CCTTC 5 cut(s) 138, 452, 498, 745, 776
HpyCH4III ACNGT 4 cut(s) 210, 383, 486, 602
HpyCH4IV ACGT 1 cut(s) 762
HpyCH4V TGCA 3 cut(s) 344, 481, 618
HpyF10VI GCNNNNNNNGC 3 cut(s) 99, 407, 847
HpyF3I CTNAG 2 cut(s) 299, 429
HpySE526I ACGT 1 cut(s) 762
Hsp92I GRCGYC 3 cut(s) 120, 351, 548
Hsp92II CATG 5 cut(s) 227, 281, 326, 571, 797
HspAI GCGC 4 cut(s) 61, 100, 303, 548
KasI GGCGCC 1 cut(s) 547
KroI GCCGGC 1 cut(s) 347
KroNI GCCGGC 1 cut(s) 349
Ksp22I TGATCA 2 cut(s) 604, 652
Kzo9I GATC 9 cut(s) 31, 49, 136, 360, 562, 604, 652, 711, 715
LmnI GCTCC 1 cut(s) 261
Lsp1109I GCAGC 6 cut(s) 29, 32, 102, 317, 356, 412
LweI GCATC 2 cut(s) 102, 377
MaeI CTAG 1 cut(s) 510
MaeII ACGT 1 cut(s) 762
MaeIII GTNAC 1 cut(s) 392
MalI GATC 9 cut(s) 33, 51, 138, 362, 564, 606, 654, 713, 717
MboI GATC 9 cut(s) 31, 49, 136, 360, 562, 604, 652, 711, 715
MboII GAAGA 2 cut(s) 469, 748
MhlI GDGCHC 1 cut(s) 802
MluCI AATT 1 cut(s) 643
Mly113I GGCGCC 1 cut(s) 548
MlyI GAGTC 2 cut(s) 647, 778
MmeI TCCRAC 1 cut(s) 723
MroNI GCCGGC 1 cut(s) 347
MseI TTAA 2 cut(s) 846, 865
MslI CAYNNNNRTG 1 cut(s) 670
MspA1I CMGCKG 1 cut(s) 23
MspI CCGG 2 cut(s) 42, 348
MspR9I CCNGG 1 cut(s) 199
MvaI CCWGG 1 cut(s) 199
MvnI CGCG 1 cut(s) 100
MwoI GCNNNNNNNGC 3 cut(s) 99, 407, 847
NaeI GCCGGC 1 cut(s) 349
NarI GGCGCC 1 cut(s) 548
NcoI CCATGG 1 cut(s) 277
NdeII GATC 9 cut(s) 31, 49, 136, 360, 562, 604, 652, 711, 715
NgoMIV GCCGGC 1 cut(s) 347
NlaIII CATG 5 cut(s) 227, 281, 326, 571, 797
NlaIV GGNNCC 1 cut(s) 549
OliI CACNNNNGTG 1 cut(s) 670
PcsI WCGNNNNNNNCGW 1 cut(s) 372
PdiI GCCGGC 1 cut(s) 349
PfeI GAWTC 2 cut(s) 26, 499
PflMI CCANNNNNTGG 1 cut(s) 229
PkrI GCNGC 7 cut(s) 19, 22, 92, 307, 346, 424, 427
Ple19I CGATCG 1 cut(s) 718
PleI GAGTC 2 cut(s) 646, 777
PluTI GGCGCC 1 cut(s) 551
PmaCI CACGTG 1 cut(s) 763
PmlI CACGTG 1 cut(s) 763
PpsI GAGTC 2 cut(s) 646, 777
Ppu21I YACGTR 1 cut(s) 763
Psp6I CCWGG 1 cut(s) 197
PspCI CACGTG 1 cut(s) 763
PspFI CCCAGC 1 cut(s) 386
PspGI CCWGG 1 cut(s) 197
PspN4I GGNNCC 1 cut(s) 549
PspPI GGNCC 1 cut(s) 232
PvuI CGATCG 1 cut(s) 718
RsaI GTAC 3 cut(s) 228, 380, 759
RsaNI GTAC 3 cut(s) 227, 379, 758
RseI CAYNNNNRTG 1 cut(s) 670
SaqAI TTAA 2 cut(s) 846, 865
SatI GCNGC 7 cut(s) 18, 21, 91, 306, 345, 423, 426
Sau3AI GATC 9 cut(s) 31, 49, 136, 360, 562, 604, 652, 711, 715
Sau96I GGNCC 1 cut(s) 232
SchI GAGTC 2 cut(s) 647, 778
ScrFI CCNGG 1 cut(s) 199
SduI GDGCHC 1 cut(s) 802
SetI ASST 8 cut(s) 200, 242, 258, 444, 527, 732, 765, 790
SfaNI GCATC 2 cut(s) 102, 377
SfoI GGCGCC 1 cut(s) 549
SinI GGWCC 1 cut(s) 232
SmiMI CAYNNNNRTG 1 cut(s) 670
SmlI CTYRAG 1 cut(s) 801
SmoI CTYRAG 1 cut(s) 801
SpeI ACTAGT 1 cut(s) 509
Sse9I AATT 1 cut(s) 643
SsiI CCGC 6 cut(s) 23, 125, 220, 410, 422, 629
SspDI GGCGCC 1 cut(s) 547
SspMI CTAG 1 cut(s) 510
StyD4I CCNGG 1 cut(s) 197
StyI CCWWGG 3 cut(s) 165, 277, 825
TaaI ACNGT 4 cut(s) 210, 383, 486, 602
TaiI ACGT 1 cut(s) 765
TaqI TCGA 4 cut(s) 205, 375, 502, 714
TasI AATT 1 cut(s) 643
TatI WGTACW 3 cut(s) 226, 378, 757
TauI GCSGC 1 cut(s) 425
TfiI GAWTC 2 cut(s) 26, 499
Tru1I TTAA 2 cut(s) 846, 865
Tru9I TTAA 2 cut(s) 846, 865
TscAI CASTG 2 cut(s) 388, 714
TseI GCWGC 6 cut(s) 17, 20, 90, 305, 344, 425
TspDTI ATGAA 4 cut(s) 24, 120, 280, 844
TspRI CASTG 2 cut(s) 388, 714
Van91I CCANNNNNTGG 1 cut(s) 229
VpaK11BI GGWCC 1 cut(s) 232
XspI CTAG 1 cut(s) 510
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.