Rmu_ssc0000116.1_g000036

alpha/beta hydrolase fold

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000116.1
OG0000860
Physical Location & Seq
Forward (+)
180028 .. 181869
1842 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_ssc0000116.1_g000036.1.cds

Sequence Viewer

Length: 1389 bp
atgtcaaaacctgatcaattcaaacccaacatcatctccaatccagatggaacctacacgcgcctcttaaaatttccaagcaccgaggccgactctacctcagccactaaccatgtactctccaaagacatccctgtaaaccctgaaacccaaaccatggtccgactcttcctccctcggaatgtccttggctccaaaaccaaactccctctcatattttactaccacggtggtgcgtttctctatttcagcgcaactacaactgttttccatgagttttgttccaacatggctttggaactccaagctattgttgtctccttggactaccgtcttgcccctgaacaccgcctcccggcagcctacgaggatgccatagatgcgttgcactggatcaagaccacagaggaagagtgggtaagagaatttgctgatttctcaagctgttttttaatgggtactagttctggtggaaacctagcttaccatgctgggcttcttgcttgtgaagttagagatcaccttgggcctttggaaatcaaaggactgatattgcaccatcccttttttggtggatccgaaaggactgagtcggaactgaggatggagcatgatcgaattttgccactacgggcaagcgatggagcgtggaaattggcgttgcctattggtgctgaccgtgatcatgagtattgcaatccaacagtgggcgatagagatggtatttgggctgcgattcgggccatggattgtagggtcttggttctgggttgttatggagatccgatgattgagcgacagatggggttggcgaaaatggtagaggagaagggtgtacgaacggtgactcattttggcgatggttgtcatggtttggaaataacggacgggtccaaagctccggggttttggtatcctcttcctctgagcggcggattctcgcattctcgttgttgttcgtcaatggtggtgatggcatgttttggtctgcttatcctcttcttgattgcgttgggggacgttgatgttatgttgagttcaggggttggaggtgatgttgtttctgcgtcgaagcccgatggtgaagtgaaagatgcagccttgtgggtggggatggcatctagattgcagacgagcagtgtcgatctttggtgtgccggtgattttgctgtcatggacggtgatggtatgctggtttgctcgaactcagggactactgcttttgatggtggtgctcataggtataacggctgcagtatggaggcagaggcggctgtcaaatcacaccaggtgacgggttctacaatgttggtggcggcggcgacgacttgctggttggtgacgacggtgctctggttcaatgaggtggaacttatggggtgcccttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

462

Amino Acids

50.29

Weight (kDa)

5.17

Isoelectric Point (pI)

30.46

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1380
AccB7I CCANNNNNTGG 1 cut(s) 157
AccBSI CCGCTC 1 cut(s) 930
AccII CGCG 1 cut(s) 61
AciI CCGC 6 cut(s) 349, 930, 933, 1271, 1316, 1319
AclWI GGATC 4 cut(s) 401, 570, 583, 776
AcsI RAATTY 3 cut(s) 71, 425, 618
AdeI CACNNNGTG 1 cut(s) 1291
AfaI GTAC 3 cut(s) 117, 460, 837
AfiI CCNNNNNNNGG 6 cut(s) 157, 355, 569, 707, 929, 1294
AgsI TTSAA 2 cut(s) 22, 1360
AhlI ACTAGT 1 cut(s) 461
AjnI CCWGG 1 cut(s) 1287
AleI CACNNNNGTG 1 cut(s) 231
AloI GAACNNNNNNTCC 2 cut(s) 336, 368
AluBI AGCT 4 cut(s) 308, 444, 482, 899
AluI AGCT 4 cut(s) 308, 444, 482, 899
Alw21I GWGCWC 2 cut(s) 1237, 1353
Alw26I GTCTC 1 cut(s) 322
AlwI GGATC 4 cut(s) 401, 570, 583, 776
AoxI GGCC 3 cut(s) 87, 527, 741
ApeKI GCWGC 4 cut(s) 359, 731, 1097, 1251
ApoI RAATTY 3 cut(s) 71, 425, 618
AspLEI GCGC 2 cut(s) 63, 254
AspS9I GGNCC 4 cut(s) 160, 527, 741, 891
AsuC2I CCSGG 2 cut(s) 356, 903
AsuHPI GGTGA 9 cut(s) 512, 856, 982, 1064, 1094, 1172, 1193, 1303, 1351
AvaII GGWCC 2 cut(s) 160, 891
BaeGI GKGCMC 1 cut(s) 1385
BamHI GGATCC 1 cut(s) 575
BanI GGYRCC 1 cut(s) 1380
Bbv12I GWGCWC 2 cut(s) 1237, 1353
BbvCI CCTCAGC 1 cut(s) 100
BbvI GCAGC 4 cut(s) 371, 718, 1109, 1238
BceAI ACGGC 1 cut(s) 1264
BciT130I CCWGG 1 cut(s) 1289
BciVI GTATCC 1 cut(s) 924
BclI TGATCA 2 cut(s) 13, 682
BcnI CCSGG 2 cut(s) 356, 903
BcoDI GTCTC 1 cut(s) 322
BcuI ACTAGT 1 cut(s) 461
BfaI CTAG 3 cut(s) 462, 479, 1122
BfmI CTRYAG 1 cut(s) 1252
BfuI GTATCC 1 cut(s) 924
BisI GCNGC 8 cut(s) 360, 732, 931, 1098, 1252, 1272, 1317, 1320
BlsI GCNGC 8 cut(s) 361, 733, 932, 1099, 1253, 1273, 1318, 1321
Bme1390I CCNGG 3 cut(s) 356, 903, 1289
Bme18I GGWCC 2 cut(s) 160, 891
BmgT120I GGNCC 4 cut(s) 160, 527, 741, 891
BmiI GGNNCC 5 cut(s) 52, 193, 577, 892, 1382
BmrFI CCNGG 3 cut(s) 356, 903, 1289
BmsI GCATC 4 cut(s) 361, 370, 1084, 1127
BoxI GACNNNNGTC 1 cut(s) 330
BplI GAGNNNNNCTC 2 cut(s) 77, 109
Bpu10I CCTNAGC 1 cut(s) 100
BpuEI CTTGAG 1 cut(s) 424
BpuMI CCSGG 2 cut(s) 356, 903
BsaJI CCNNGG 9 cut(s) 84, 156, 176, 187, 226, 321, 523, 744, 902
BsaXI ACNNNNNCTCC 8 cut(s) 20, 50, 156, 186, 336, 366, 1041, 1071
Bsc4I CCNNNNNNNGG 6 cut(s) 157, 355, 569, 707, 929, 1294
Bse118I RCCGGY 1 cut(s) 1157
Bse1I ACTGG 1 cut(s) 395
BseBI CCWGG 1 cut(s) 1289
BseDI CCNNGG 9 cut(s) 84, 156, 176, 187, 226, 321, 523, 744, 902
BseGI GGATG 5 cut(s) 129, 376, 559, 609, 1119
BseLI CCNNNNNNNGG 6 cut(s) 157, 355, 569, 707, 929, 1294
BseMII CTCAG 5 cut(s) 114, 579, 590, 917, 1221
BseNI ACTGG 1 cut(s) 395
BseRI GAGGAG 1 cut(s) 839
BseSI GKGCMC 1 cut(s) 1385
BseXI GCAGC 4 cut(s) 371, 718, 1109, 1238
BseYI CCCAGC 1 cut(s) 491
Bsh1236I CGCG 1 cut(s) 61
BshFI GGCC 3 cut(s) 89, 529, 743
BshNI GGYRCC 1 cut(s) 1380
BsiHKAI GWGCWC 2 cut(s) 1237, 1353
BsiSI CCGG 3 cut(s) 356, 902, 1158
BslFI GGGAC 2 cut(s) 1031, 1225
BslI CCNNNNNNNGG 6 cut(s) 157, 355, 569, 707, 929, 1294
BsmAI GTCTC 1 cut(s) 322
BsmFI GGGAC 2 cut(s) 1031, 1225
BsmI GAATGC 1 cut(s) 943
BsnI GGCC 3 cut(s) 89, 529, 743
Bsp1286I GDGCHC 3 cut(s) 1237, 1353, 1385
Bsp143I GATC 8 cut(s) 13, 393, 517, 575, 613, 682, 781, 1144
Bsp19I CCATGG 2 cut(s) 156, 744
BspACI CCGC 6 cut(s) 349, 930, 933, 1271, 1316, 1319
BspANI GGCC 3 cut(s) 89, 529, 743
BspCNI CTCAG 5 cut(s) 113, 580, 591, 918, 1220
BspFNI CGCG 1 cut(s) 61
BspHI TCATGA 1 cut(s) 685
BspLI GGNNCC 5 cut(s) 52, 193, 577, 892, 1382
BspMAI CTGCAG 1 cut(s) 1256
BspPI GGATC 4 cut(s) 401, 570, 583, 776
BspT107I GGYRCC 1 cut(s) 1380
BsrBI CCGCTC 1 cut(s) 930
BsrFI RCCGGY 1 cut(s) 1157
BsrI ACTGG 1 cut(s) 395
BssAI RCCGGY 1 cut(s) 1157
BssECI CCNNGG 9 cut(s) 84, 156, 176, 187, 226, 321, 523, 744, 902
BssMI GATC 8 cut(s) 13, 393, 517, 575, 613, 682, 781, 1144
BssT1I CCWWGG 5 cut(s) 156, 187, 321, 523, 744
Bst2UI CCWGG 1 cut(s) 1289
Bst4CI ACNGT 8 cut(s) 230, 265, 332, 680, 706, 844, 1181, 1348
Bst6I CTCTTC 4 cut(s) 173, 405, 924, 1004
BstC8I GCNNGC 1 cut(s) 637
BstDEI CTNAG 5 cut(s) 100, 588, 599, 926, 1207
BstDSI CCRYGG 3 cut(s) 156, 226, 744
BstF5I GGATG 5 cut(s) 129, 376, 559, 609, 1119
BstFNI CGCG 1 cut(s) 61
BstHHI GCGC 2 cut(s) 63, 254
BstKTI GATC 8 cut(s) 16, 396, 520, 578, 616, 685, 784, 1147
BstMAI GTCTC 1 cut(s) 322
BstMBI GATC 8 cut(s) 13, 393, 517, 575, 613, 682, 781, 1144
BstMWI GCNNNNNNNGC 4 cut(s) 380, 488, 740, 1271
BstNI CCWGG 1 cut(s) 1289
BstNSI RCATGY 1 cut(s) 981
BstPAI GACNNNNGTC 1 cut(s) 330
BstSCI CCNGG 3 cut(s) 354, 901, 1287
BstSFI CTRYAG 1 cut(s) 1252
BstSLI GKGCMC 1 cut(s) 1385
BstUI CGCG 1 cut(s) 61
BstV1I GCAGC 4 cut(s) 371, 718, 1109, 1238
BstX2I RGATCY 2 cut(s) 575, 781
BstYI RGATCY 2 cut(s) 575, 781
BsuI GTATCC 1 cut(s) 924
BsuRI GGCC 3 cut(s) 89, 529, 743
BtgI CCRYGG 3 cut(s) 156, 226, 744
BtgZI GCGATG 2 cut(s) 654, 873
BtsCI GGATG 5 cut(s) 129, 376, 559, 609, 1119
BtsI GCAGTG 1 cut(s) 1144
BtsIMutI CAGTG 3 cut(s) 388, 711, 1144
Cac8I GCNNGC 1 cut(s) 637
CciI TCATGA 1 cut(s) 685
CfoI GCGC 2 cut(s) 63, 254
Cfr10I RCCGGY 1 cut(s) 1157
Cfr13I GGNCC 4 cut(s) 160, 527, 741, 891
CseI GACGC 1 cut(s) 1056
CsiI ACCWGGT 1 cut(s) 1287
Csp6I GTAC 3 cut(s) 116, 459, 836
CspCI CAANNNNNGTGG 2 cut(s) 1293, 1328
CviQI GTAC 3 cut(s) 116, 459, 836
DdeI CTNAG 5 cut(s) 100, 588, 599, 926, 1207
DpnI GATC 8 cut(s) 15, 395, 519, 577, 615, 684, 783, 1146
DpnII GATC 8 cut(s) 13, 393, 517, 575, 613, 682, 781, 1144
DraIII CACNNNGTG 1 cut(s) 1291
Eam1104I CTCTTC 4 cut(s) 173, 405, 924, 1004
EarI CTCTTC 4 cut(s) 173, 405, 924, 1004
EciI GGCGGA 1 cut(s) 948
Eco130I CCWWGG 5 cut(s) 156, 187, 321, 523, 744
Eco47I GGWCC 2 cut(s) 160, 891
EcoRII CCWGG 1 cut(s) 1287
EcoT14I CCWWGG 5 cut(s) 156, 187, 321, 523, 744
ErhI CCWWGG 5 cut(s) 156, 187, 321, 523, 744
FaqI GGGAC 2 cut(s) 1031, 1225
FbaI TGATCA 2 cut(s) 13, 682
Fnu4HI GCNGC 8 cut(s) 360, 732, 931, 1098, 1252, 1272, 1317, 1320
FokI GGATG 5 cut(s) 116, 383, 546, 616, 1126
Fsp4HI GCNGC 8 cut(s) 360, 732, 931, 1098, 1252, 1272, 1317, 1320
FspBI CTAG 3 cut(s) 462, 479, 1122
GlaI GCGC 2 cut(s) 62, 253
GluI GCNGC 8 cut(s) 360, 732, 931, 1098, 1252, 1272, 1317, 1320
GsaI CCCAGC 1 cut(s) 495
HaeIII GGCC 3 cut(s) 89, 529, 743
HapII CCGG 3 cut(s) 356, 902, 1158
HgaI GACGC 1 cut(s) 1056
HhaI GCGC 2 cut(s) 63, 254
Hin6I GCGC 2 cut(s) 61, 252
HinP1I GCGC 2 cut(s) 61, 252
HinfI GANTC 6 cut(s) 92, 165, 590, 736, 847, 936
HpaII CCGG 3 cut(s) 356, 902, 1158
HphI GGTGA 9 cut(s) 512, 856, 982, 1064, 1094, 1172, 1193, 1303, 1351
Hpy166II GTNNAC 2 cut(s) 139, 836
Hpy188I TCNGA 6 cut(s) 164, 180, 580, 595, 786, 927
Hpy188III TCNNGA 5 cut(s) 44, 397, 686, 1003, 1122
Hpy8I GTNNAC 2 cut(s) 139, 836
Hpy99I CGWCG 3 cut(s) 1072, 1327, 1348
HpyAV CCTTC 1 cut(s) 823
HpyCH4III ACNGT 8 cut(s) 230, 265, 332, 680, 706, 844, 1181, 1348
HpyCH4IV ACGT 1 cut(s) 1020
HpyCH4V TGCA 6 cut(s) 388, 556, 696, 1097, 1129, 1254
HpyF10VI GCNNNNNNNGC 4 cut(s) 380, 488, 740, 1271
HpyF3I CTNAG 5 cut(s) 100, 588, 599, 926, 1207
HpySE526I ACGT 1 cut(s) 1020
HspAI GCGC 2 cut(s) 61, 252
Ksp22I TGATCA 2 cut(s) 13, 682
Kzo9I GATC 8 cut(s) 13, 393, 517, 575, 613, 682, 781, 1144
LmnI GCTCC 4 cut(s) 197, 607, 644, 904
Lsp1109I GCAGC 4 cut(s) 371, 718, 1109, 1238
LweI GCATC 4 cut(s) 361, 370, 1084, 1127
MabI ACCWGGT 1 cut(s) 1287
MaeI CTAG 3 cut(s) 462, 479, 1122
MaeII ACGT 1 cut(s) 1020
MaeIII GTNAC 3 cut(s) 844, 1291, 1339
MalI GATC 8 cut(s) 15, 395, 519, 577, 615, 684, 783, 1146
MbiI CCGCTC 1 cut(s) 930
MboI GATC 8 cut(s) 13, 393, 517, 575, 613, 682, 781, 1144
MboII GAAGA 4 cut(s) 160, 422, 911, 991
MflI RGATCY 2 cut(s) 575, 781
MhlI GDGCHC 3 cut(s) 1237, 1353, 1385
MluCI AATT 5 cut(s) 17, 71, 425, 618, 653
MlyI GAGTC 4 cut(s) 86, 159, 599, 841
MmeI TCCRAC 5 cut(s) 187, 309, 573, 725, 1027
MseI TTAA 3 cut(s) 68, 452, 1387
MslI CAYNNNNRTG 1 cut(s) 231
MspI CCGG 3 cut(s) 356, 902, 1158
MspR9I CCNGG 3 cut(s) 356, 903, 1289
Mva1269I GAATGC 1 cut(s) 943
MvaI CCWGG 1 cut(s) 1289
MvnI CGCG 1 cut(s) 61
MwoI GCNNNNNNNGC 4 cut(s) 380, 488, 740, 1271
NciI CCSGG 2 cut(s) 356, 903
NcoI CCATGG 2 cut(s) 156, 744
NdeII GATC 8 cut(s) 13, 393, 517, 575, 613, 682, 781, 1144
NlaIV GGNNCC 5 cut(s) 52, 193, 577, 892, 1382
NmuCI GTSAC 3 cut(s) 844, 1291, 1339
NspI RCATGY 1 cut(s) 981
OliI CACNNNNGTG 1 cut(s) 231
PagI TCATGA 1 cut(s) 685
PctI GAATGC 1 cut(s) 943
PfeI GAWTC 2 cut(s) 736, 936
PflFI GACNNNGTC 1 cut(s) 589
PflMI CCANNNNNTGG 1 cut(s) 157
PkrI GCNGC 8 cut(s) 361, 733, 932, 1099, 1253, 1273, 1318, 1321
PleI GAGTC 4 cut(s) 86, 159, 598, 841
PpsI GAGTC 4 cut(s) 86, 159, 598, 841
PshAI GACNNNNGTC 1 cut(s) 330
Psp6I CCWGG 1 cut(s) 1287
PspFI CCCAGC 1 cut(s) 491
PspGI CCWGG 1 cut(s) 1287
PspN4I GGNNCC 5 cut(s) 52, 193, 577, 892, 1382
PspPI GGNCC 4 cut(s) 160, 527, 741, 891
PstI CTGCAG 1 cut(s) 1256
PsuI RGATCY 2 cut(s) 575, 781
PsyI GACNNNGTC 1 cut(s) 589
RsaI GTAC 3 cut(s) 117, 460, 837
RsaNI GTAC 3 cut(s) 116, 459, 836
RseI CAYNNNNRTG 1 cut(s) 231
SaqAI TTAA 3 cut(s) 68, 452, 1387
SatI GCNGC 8 cut(s) 360, 732, 931, 1098, 1252, 1272, 1317, 1320
Sau3AI GATC 8 cut(s) 13, 393, 517, 575, 613, 682, 781, 1144
Sau96I GGNCC 4 cut(s) 160, 527, 741, 891
SchI GAGTC 4 cut(s) 86, 159, 599, 841
ScrFI CCNGG 3 cut(s) 356, 903, 1289
SduI GDGCHC 3 cut(s) 1237, 1353, 1385
SexAI ACCWGGT 1 cut(s) 1287
SfaNI GCATC 4 cut(s) 361, 370, 1084, 1127
SfcI CTRYAG 1 cut(s) 1252
SinI GGWCC 2 cut(s) 160, 891
SmiMI CAYNNNNRTG 1 cut(s) 231
SmlI CTYRAG 1 cut(s) 439
SmoI CTYRAG 1 cut(s) 439
SpeI ACTAGT 1 cut(s) 461
Sse9I AATT 5 cut(s) 17, 71, 425, 618, 653
SsiI CCGC 6 cut(s) 349, 930, 933, 1271, 1316, 1319
SspMI CTAG 3 cut(s) 462, 479, 1122
StyD4I CCNGG 3 cut(s) 354, 901, 1287
StyI CCWWGG 5 cut(s) 156, 187, 321, 523, 744
TaaI ACNGT 8 cut(s) 230, 265, 332, 680, 706, 844, 1181, 1348
TaiI ACGT 1 cut(s) 1023
TaqI TCGA 4 cut(s) 616, 1070, 1143, 1202
TasI AATT 5 cut(s) 17, 71, 425, 618, 653
TatI WGTACW 1 cut(s) 115
TauI GCSGC 4 cut(s) 933, 1274, 1319, 1322
TfiI GAWTC 2 cut(s) 736, 936
Tru1I TTAA 3 cut(s) 68, 452, 1387
Tru9I TTAA 3 cut(s) 68, 452, 1387
TscAI CASTG 3 cut(s) 395, 711, 1144
TseFI GTSAC 3 cut(s) 844, 1291, 1339
TseI GCWGC 4 cut(s) 359, 731, 1097, 1251
Tsp45I GTSAC 3 cut(s) 844, 1291, 1339
TspGWI ACGGA 1 cut(s) 899
TspRI CASTG 3 cut(s) 395, 711, 1144
Tth111I GACNNNGTC 1 cut(s) 589
Van91I CCANNNNNTGG 1 cut(s) 157
VpaK11BI GGWCC 2 cut(s) 160, 891
XapI RAATTY 3 cut(s) 71, 425, 618
XbaI TCTAGA 1 cut(s) 1121
XceI RCATGY 1 cut(s) 981
XcmI CCANNNNNNNNNTGG 2 cut(s) 292, 566
XspI CTAG 3 cut(s) 462, 479, 1122
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.