Rroxscaffold_4G00325300

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000860
Physical Location & Seq
Reverse (-)
57019893 .. 57020767
875 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00325300.1

Sequence Viewer

Length: 780 bp
ATGGCTTCAAAGATGATTCAACTCAAATCGGCAAACAAGACCAATAAGGTAGCTACTTCAAAAATGAGTAGAAACCTTAACAATCATACCGGTCAAAGTGAGCCGTCTCAACTCGCGACATTCATAACTCATCCATCTCGGTCACGTCGGCCGATCATCACTTCGACAACAATGGGGCCAAACCTCACTAAGAAAAGATGGTCGGCAAGCTCAAGCGACTCAAGGGAACATTCTTCTTTGCACACCGCCGGTGTTGATCAGCGTACACACCTACGGCCCGACGACTCAAATAGCCCAATTGGTGAAGCGGTTGAGAAGCTACAGAAAACTATTGAAGAGAAGGGTATGAAAATTGCTGCGCTCATGGAGAAGTTAGAAACTCAACGTGGCGATGACTCGAGAAAAGGTCCGCGTGGTCATAAGAAAGACTCATCTAGCAGATCATCTTCTCGAACAAAAAGAAGAATCAAATTCCATTCTAGCTTCATGGTCAATTCAACAACTTCAAGACACGATTGCCAACACGATCGAGCTCAATATGGTGGTCCCTCTCGGGATACACTAACGTATTCCAAACCTTATACGAAGCGGCTCGATAATTTGAGGATGCCAACGGATACCGACCTCCCAAGTTTCAACAATTCGATGGCAAAGGTGACCCCGAAGCAACACATTGCGCATTTCGTCGAGACATGCAATAGCGGCGGCACGGATGGCGACTATCTTGTGAAACAATTCATTCGCTCACTCGAGGGACAAGCATTCGAATGGTTTACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Retrotrans_gag

Protein Analysis

259

Amino Acids

29.13

Weight (kDa)

10.06

Isoelectric Point (pI)

60.8

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 678
AccII CGCG 2 cut(s) 116, 412
AciI CCGC 6 cut(s) 246, 308, 410, 589, 702, 705
AcoI YGGCCR 1 cut(s) 149
AcsI RAATTY 1 cut(s) 470
AfaI GTAC 1 cut(s) 265
AgeI ACCGGT 1 cut(s) 89
AgsI TTSAA 7 cut(s) 9, 20, 60, 335, 498, 507, 637
AjiI CACGTC 1 cut(s) 146
AluBI AGCT 5 cut(s) 53, 210, 319, 483, 533
AluI AGCT 5 cut(s) 53, 210, 319, 483, 533
Alw21I GWGCWC 1 cut(s) 535
Alw26I GTCTC 2 cut(s) 111, 683
Ama87I CYCGRG 3 cut(s) 397, 552, 749
AoxI GGCC 3 cut(s) 149, 176, 275
ApeKI GCWGC 1 cut(s) 356
ApoI RAATTY 1 cut(s) 470
AsiGI ACCGGT 1 cut(s) 89
Asp700I GAANNNNTTC 1 cut(s) 734
AspLEI GCGC 2 cut(s) 361, 679
AspS9I GGNCC 4 cut(s) 176, 276, 407, 545
AsuHPI GGTGA 2 cut(s) 314, 667
AsuII TTCGAA 1 cut(s) 765
AvaI CYCGRG 3 cut(s) 397, 552, 749
AvaII GGWCC 2 cut(s) 407, 545
BanII GRGCYC 1 cut(s) 535
Bbv12I GWGCWC 1 cut(s) 535
BbvI GCAGC 1 cut(s) 343
BccI CCATC 4 cut(s) 142, 192, 640, 707
BceAI ACGGC 2 cut(s) 88, 290
BciVI GTATCC 2 cut(s) 550, 610
BclI TGATCA 1 cut(s) 256
BcoDI GTCTC 2 cut(s) 111, 683
BfaI CTAG 2 cut(s) 435, 480
BfmI CTRYAG 1 cut(s) 320
BfuI GTATCC 2 cut(s) 550, 610
BisI GCNGC 4 cut(s) 357, 590, 703, 706
BlsI GCNGC 4 cut(s) 358, 591, 704, 707
Bme18I GGWCC 2 cut(s) 407, 545
BmeT110I CYCGRG 3 cut(s) 397, 552, 749
BmgBI CACGTC 1 cut(s) 146
BmgT120I GGNCC 4 cut(s) 176, 276, 407, 545
BmiI GGNNCC 2 cut(s) 177, 547
BmsI GCATC 1 cut(s) 597
Bpu14I TTCGAA 1 cut(s) 765
BpuEI CTTGAG 2 cut(s) 196, 205
BsaWI WCCGGW 1 cut(s) 89
Bse118I RCCGGY 2 cut(s) 89, 248
Bse3DI GCAATG 1 cut(s) 672
BseGI GGATG 3 cut(s) 130, 612, 718
BseMI GCAATG 1 cut(s) 672
BseX3I CGGCCG 1 cut(s) 149
BseXI GCAGC 1 cut(s) 343
Bsh1236I CGCG 2 cut(s) 116, 412
Bsh1285I CGRYCG 2 cut(s) 152, 529
BshFI GGCC 3 cut(s) 151, 178, 277
BshTI ACCGGT 1 cut(s) 89
BsiEI CGRYCG 2 cut(s) 152, 529
BsiHKAI GWGCWC 1 cut(s) 535
BsiHKCI CYCGRG 3 cut(s) 397, 552, 749
BsiSI CCGG 2 cut(s) 90, 249
BslFI GGGAC 2 cut(s) 531, 768
BsmAI GTCTC 2 cut(s) 111, 683
BsmBI CGTCTC 1 cut(s) 111
BsmFI GGGAC 2 cut(s) 531, 768
BsmI GAATGC 1 cut(s) 761
BsnI GGCC 3 cut(s) 151, 178, 277
BsoBI CYCGRG 3 cut(s) 397, 552, 749
Bsp119I TTCGAA 1 cut(s) 765
Bsp1286I GDGCHC 1 cut(s) 535
Bsp143I GATC 4 cut(s) 153, 256, 440, 526
Bsp68I TCGCGA 1 cut(s) 116
BspACI CCGC 6 cut(s) 246, 308, 410, 589, 702, 705
BspANI GGCC 3 cut(s) 151, 178, 277
BspFNI CGCG 2 cut(s) 116, 412
BspLI GGNNCC 2 cut(s) 177, 547
BspT104I TTCGAA 1 cut(s) 765
BsrDI GCAATG 1 cut(s) 672
BsrFI RCCGGY 2 cut(s) 89, 248
BssAI RCCGGY 2 cut(s) 89, 248
BssMI GATC 4 cut(s) 153, 256, 440, 526
Bst6I CTCTTC 1 cut(s) 330
BstBI TTCGAA 1 cut(s) 765
BstC8I GCNNGC 1 cut(s) 208
BstDEI CTNAG 1 cut(s) 189
BstEII GGTNACC 1 cut(s) 655
BstF5I GGATG 3 cut(s) 130, 612, 718
BstFNI CGCG 2 cut(s) 116, 412
BstHHI GCGC 2 cut(s) 361, 679
BstKTI GATC 4 cut(s) 156, 259, 443, 529
BstMAI GTCTC 2 cut(s) 111, 683
BstMBI GATC 4 cut(s) 153, 256, 440, 526
BstMCI CGRYCG 2 cut(s) 152, 529
BstMWI GCNNNNNNNGC 2 cut(s) 702, 714
BstNSI RCATGY 1 cut(s) 696
BstPI GGTNACC 1 cut(s) 655
BstSFI CTRYAG 1 cut(s) 320
BstUI CGCG 2 cut(s) 116, 412
BstV1I GCAGC 1 cut(s) 343
BstZI CGGCCG 1 cut(s) 149
BsuI GTATCC 2 cut(s) 550, 610
BsuRI GGCC 3 cut(s) 151, 178, 277
BtgZI GCGATG 1 cut(s) 405
BtrI CACGTC 1 cut(s) 146
BtsCI GGATG 3 cut(s) 130, 612, 718
BtuMI TCGCGA 1 cut(s) 116
Cac8I GCNNGC 1 cut(s) 208
CfoI GCGC 2 cut(s) 361, 679
Cfr10I RCCGGY 2 cut(s) 89, 248
Cfr13I GGNCC 4 cut(s) 176, 276, 407, 545
Csp6I GTAC 1 cut(s) 264
CspAI ACCGGT 1 cut(s) 89
CviAII CATG 3 cut(s) 364, 487, 693
CviQI GTAC 1 cut(s) 264
DdeI CTNAG 1 cut(s) 189
DpnI GATC 4 cut(s) 155, 258, 442, 528
DpnII GATC 4 cut(s) 153, 256, 440, 526
EaeI YGGCCR 1 cut(s) 149
EagI CGGCCG 1 cut(s) 149
Eam1104I CTCTTC 1 cut(s) 330
EarI CTCTTC 1 cut(s) 330
Ecl136II GAGCTC 1 cut(s) 533
EclXI CGGCCG 1 cut(s) 149
Eco24I GRGCYC 1 cut(s) 535
Eco47I GGWCC 2 cut(s) 407, 545
Eco52I CGGCCG 1 cut(s) 149
Eco53kI GAGCTC 1 cut(s) 533
Eco88I CYCGRG 3 cut(s) 397, 552, 749
Eco91I GGTNACC 1 cut(s) 655
EcoICRI GAGCTC 1 cut(s) 533
EcoO65I GGTNACC 1 cut(s) 655
EcoT38I GRGCYC 1 cut(s) 535
Esp3I CGTCTC 1 cut(s) 111
FaeI CATG 3 cut(s) 367, 490, 696
FaqI GGGAC 2 cut(s) 531, 768
FatI CATG 3 cut(s) 363, 486, 692
FbaI TGATCA 1 cut(s) 256
Fnu4HI GCNGC 4 cut(s) 357, 590, 703, 706
FokI GGATG 3 cut(s) 117, 619, 725
FriOI GRGCYC 1 cut(s) 535
Fsp4HI GCNGC 4 cut(s) 357, 590, 703, 706
FspBI CTAG 2 cut(s) 435, 480
FspI TGCGCA 1 cut(s) 678
GlaI GCGC 2 cut(s) 360, 678
GluI GCNGC 4 cut(s) 357, 590, 703, 706
HaeIII GGCC 3 cut(s) 151, 178, 277
HapII CCGG 2 cut(s) 90, 249
HhaI GCGC 2 cut(s) 361, 679
Hin1II CATG 3 cut(s) 367, 490, 696
Hin6I GCGC 2 cut(s) 359, 677
HinP1I GCGC 2 cut(s) 359, 677
HinfI GANTC 6 cut(s) 16, 218, 284, 395, 428, 465
HpaII CCGG 2 cut(s) 90, 249
HphI GGTGA 2 cut(s) 314, 667
Hpy166II GTNNAC 2 cut(s) 266, 774
Hpy188III TCNNGA 6 cut(s) 115, 399, 450, 507, 554, 688
Hpy8I GTNNAC 2 cut(s) 266, 774
Hpy99I CGWCG 3 cut(s) 150, 284, 689
HpyAV CCTTC 1 cut(s) 334
HpyCH4IV ACGT 3 cut(s) 145, 385, 566
HpyCH4V TGCA 2 cut(s) 241, 696
HpyF10VI GCNNNNNNNGC 2 cut(s) 702, 714
HpyF3I CTNAG 1 cut(s) 189
HpySE526I ACGT 3 cut(s) 145, 385, 566
Hsp92II CATG 3 cut(s) 367, 490, 696
HspAI GCGC 2 cut(s) 359, 677
Ksp22I TGATCA 1 cut(s) 256
Kzo9I GATC 4 cut(s) 153, 256, 440, 526
LpnPI CCDG 2 cut(s) 103, 262
Lsp1109I GCAGC 1 cut(s) 343
LweI GCATC 1 cut(s) 597
MaeI CTAG 2 cut(s) 435, 480
MaeII ACGT 3 cut(s) 145, 385, 566
MaeIII GTNAC 2 cut(s) 141, 655
MalI GATC 4 cut(s) 155, 258, 442, 528
MboI GATC 4 cut(s) 153, 256, 440, 526
MboII GAAGA 4 cut(s) 225, 347, 438, 474
MfeI CAATTG 1 cut(s) 297
MhlI GDGCHC 1 cut(s) 535
MluCI AATT 7 cut(s) 297, 351, 470, 493, 598, 640, 734
MlyI GAGTC 4 cut(s) 212, 278, 389, 422
MnlI CCTC 5 cut(s) 194, 559, 597, 635, 745
MroXI GAANNNNTTC 1 cut(s) 734
MseI TTAA 1 cut(s) 78
MslI CAYNNNNRTG 1 cut(s) 766
MspI CCGG 2 cut(s) 90, 249
MunI CAATTG 1 cut(s) 297
Mva1269I GAATGC 1 cut(s) 761
MvnI CGCG 2 cut(s) 116, 412
MwoI GCNNNNNNNGC 2 cut(s) 702, 714
NdeII GATC 4 cut(s) 153, 256, 440, 526
NlaIII CATG 3 cut(s) 367, 490, 696
NlaIV GGNNCC 2 cut(s) 177, 547
NmuCI GTSAC 2 cut(s) 141, 655
NruI TCGCGA 1 cut(s) 116
NsbI TGCGCA 1 cut(s) 678
NspI RCATGY 1 cut(s) 696
NspV TTCGAA 1 cut(s) 765
PaeR7I CTCGAG 2 cut(s) 397, 749
PctI GAATGC 1 cut(s) 761
PdmI GAANNNNTTC 1 cut(s) 734
PfeI GAWTC 2 cut(s) 16, 465
PinAI ACCGGT 1 cut(s) 89
PkrI GCNGC 4 cut(s) 358, 591, 704, 707
Ple19I CGATCG 1 cut(s) 529
PleI GAGTC 4 cut(s) 212, 278, 389, 422
PpsI GAGTC 4 cut(s) 212, 278, 389, 422
Psp124BI GAGCTC 1 cut(s) 535
PspEI GGTNACC 1 cut(s) 655
PspN4I GGNNCC 2 cut(s) 177, 547
PspPI GGNCC 4 cut(s) 176, 276, 407, 545
PspXI VCTCGAGB 1 cut(s) 749
PvuI CGATCG 1 cut(s) 529
RruI TCGCGA 1 cut(s) 116
RsaI GTAC 1 cut(s) 265
RsaNI GTAC 1 cut(s) 264
RseI CAYNNNNRTG 1 cut(s) 766
SacI GAGCTC 1 cut(s) 535
SaqAI TTAA 1 cut(s) 78
SatI GCNGC 4 cut(s) 357, 590, 703, 706
Sau3AI GATC 4 cut(s) 153, 256, 440, 526
Sau96I GGNCC 4 cut(s) 176, 276, 407, 545
SchI GAGTC 4 cut(s) 212, 278, 389, 422
SduI GDGCHC 1 cut(s) 535
SfaNI GCATC 1 cut(s) 597
SfcI CTRYAG 1 cut(s) 320
Sfr274I CTCGAG 2 cut(s) 397, 749
SfuI TTCGAA 1 cut(s) 765
SgrAI CRCCGGYG 1 cut(s) 248
SinI GGWCC 2 cut(s) 407, 545
SlaI CTCGAG 2 cut(s) 397, 749
SmiMI CAYNNNNRTG 1 cut(s) 766
SmlI CTYRAG 4 cut(s) 211, 220, 397, 749
SmoI CTYRAG 4 cut(s) 211, 220, 397, 749
Sse9I AATT 7 cut(s) 297, 351, 470, 493, 598, 640, 734
SsiI CCGC 6 cut(s) 246, 308, 410, 589, 702, 705
SspMI CTAG 2 cut(s) 435, 480
SstI GAGCTC 1 cut(s) 535
TaiI ACGT 3 cut(s) 148, 388, 569
TaqI TCGA 9 cut(s) 164, 398, 451, 529, 594, 644, 687, 750, 765
TaqII GACCGA 1 cut(s) 129
TasI AATT 7 cut(s) 297, 351, 470, 493, 598, 640, 734
TauI GCSGC 3 cut(s) 592, 705, 708
TfiI GAWTC 2 cut(s) 16, 465
Tru1I TTAA 1 cut(s) 78
Tru9I TTAA 1 cut(s) 78
TseFI GTSAC 2 cut(s) 141, 655
TseI GCWGC 1 cut(s) 356
Tsp45I GTSAC 2 cut(s) 141, 655
TspDTI ATGAA 4 cut(s) 112, 362, 475, 727
TspGWI ACGGA 2 cut(s) 629, 725
VpaK11BI GGWCC 2 cut(s) 407, 545
XapI RAATTY 1 cut(s) 470
XceI RCATGY 1 cut(s) 696
XhoI CTCGAG 2 cut(s) 397, 749
XmnI GAANNNNTTC 1 cut(s) 734
XspI CTAG 2 cut(s) 435, 480
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.