FvH4_1g02670

Calmodulin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0000115
Physical Location & Seq
Reverse (-)
1501977 .. 1502474
498 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g02670.t1

Sequence Viewer

Length: 498 bp
ATGACAACAAAAAGGGAGCAGGAGGATCCGGAGCTGAAGCGAGTGTTTGCGACGTTCGACAAGAACAGTGATGGGTTTATAACAAAGCAGGAGCTCAAGGAGTCGCTGAAGAACATCAGGGTGTTCATGAGTGATGAGGAAGTTGATGAAATGGTGAAGAAAGTGGACGCAAACGGAGACGGGCTGATCGACTTTTCCGAGTTCTGTTTGCTCTGCCAGGGTCAGTCCATACTTAGTGGAGAAGAAGAAGCTGGAAATGAGGTGGAGCAAGAGGAGGAGCTCAAGGAGGCTTTTGATGTGTTTGACAAGGACAAAGATGGGTTGATTTCTGTGGAGGAGTTGGGGGTGGTTTTGTGCTCTTTGGGTTTGAGAGAGGGGAATAGAGTTGAGGCTTGTAAGGAAATGGTGAAGAAGGTTGATAAGGATGGAGATGGTATGGTTAATTTTGATGAGTTCAAGATGATGATGAAAGGTGGGAGGCTTCTCACAGCTTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005509 GO:0043167 GO:0043169 GO:0046872
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5

Protein Analysis

166

Amino Acids

18.66

Weight (kDa)

4.43

Isoelectric Point (pI)

43.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_1 PF00036 11 - 38 2.8e-07 EF hand domain
EF-hand_7 PF13499 11 - 72 2.1e-16 EF-hand domain pair
EF-hand_6 PF13405 11 - 38 1.2e-06 EF-hand domain
EF-hand_5 PF13202 12 - 34 9.8e-06 EF hand
EF-hand_8 PF13833 23 - 70 1.5e-07 EF-hand domain pair
EF-hand_1 PF00036 47 - 73 3.9e-07 EF hand domain
EF-hand_7 PF13499 91 - 157 6.9e-15 EF-hand domain pair
EF-hand_1 PF00036 93 - 118 1.3e-07 EF hand domain
EF-hand_6 PF13405 93 - 122 5e-08 EF-hand domain
EF-hand_5 PF13202 94 - 116 1.5e-06 EF hand
EF-hand_1 PF00036 132 - 157 4.4e-08 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000115)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G07900
fragaria_vesca FvH4_1g02670 FvH4_1g08280 FvH4_1g08280 FvH4_1g08280 FvH4_1g08280 FvH4_1g28911 FvH4_2g24551 FvH4_2g24560 FvH4_2g24560 FvH4_2g24560 FvH4_2g24560 FvH4_2g25031 FvH4_2g25040 FvH4_2g25050 FvH4_2g25080 FvH4_3g16800 FvH4_4g00540 FvH4_4g00540 FvH4_4g20540 FvH4_6g12320 FvH4_6g36580 FvH4_6g36580 FvH4_7g03240 FvH4_7g03270
malus_domestica MD02G1026800.v1.1 MD02G1278100.v1.1 MD02G1279500.v1.1 MD02G1279600.v1.1 MD02G1279800.v1.1 MD02G1279900.v1.1 MD02G1280100.v1.1 MD02G1280200.v1.1 MD02G1280500.v1.1 MD02G1280700.v1.1 MD02G1280800.v1.1 MD02G1281700.v1.1 MD02G1281800.v1.1 MD02G1281900.v1.1 MD02G1285600.v1.1 MD02G1285700.v1.1 MD03G1280700.v1.1 MD04G1005600.v1.1 MD07G1022900.v1.1 MD07G1032900.v1.1 MD07G1033000.v1.1 MD07G1046200.v1.1 MD07G1047400.v1.1 MD07G1047500.v1.1 MD07G1047600.v1.1 MD07G1047700.v1.1 MD07G1047900.v1.1 MD07G1048000.v1.1 MD07G1048100.v1.1 MD07G1050600.v1.1 MD11G1301600.v1.1 MD15G1168300.v1.1
prunus_persica Prupe.1G005300_v2.0.a1 Prupe.1G005800_v2.0.a1 Prupe.1G005800_v2.0.a1 Prupe.1G005900_v2.0.a1 Prupe.1G006000_v2.0.a1 Prupe.2G037000_v2.0.a1 Prupe.2G037400_v2.0.a1 Prupe.2G037400_v2.0.a1 Prupe.2G037400_v2.0.a1 Prupe.2G037400_v2.0.a1 Prupe.2G037400_v2.0.a1 Prupe.2G037400_v2.0.a1 Prupe.2G037400_v2.0.a1 Prupe.2G037400_v2.0.a1 Prupe.2G040200_v2.0.a1 Prupe.2G040300_v2.0.a1 Prupe.2G040400_v2.0.a1 Prupe.2G040400_v2.0.a1 Prupe.2G041000_v2.0.a1 Prupe.2G041000_v2.0.a1 Prupe.2G041000_v2.0.a1 Prupe.2G041100_v2.0.a1 Prupe.2G041100_v2.0.a1 Prupe.7G248200_v2.0.a1 Prupe.8G255200_v2.0.a1
pyrus_communis pycom01g04780 pycom02g02190 pycom02g23830 pycom02g23970 pycom02g23980 pycom02g24000 pycom02g24010 pycom02g24020 pycom02g24030 pycom02g24040 pycom02g24290 pycom02g24300 pycom02g24310 pycom06g20690 pycom07g01740 pycom07g03330 pycom07g03440 pycom07g03450 pycom07g03480 pycom07g03490 pycom11g26420
rosa_chinensis RchiOBHm_Chr1g0325381 RchiOBHm_Chr1g0325491 RchiOBHm_Chr1g0325501 RchiOBHm_Chr1g0327641 RchiOBHm_Chr1g0327651 RchiOBHm_Chr2g0087701 RchiOBHm_Chr2g0130871 RchiOBHm_Chr3g0456231 RchiOBHm_Chr4g0385991 RchiOBHm_Chr6g0276761 RchiOBHm_Chr6g0285221 RchiOBHm_Chr6g0293011 RchiOBHm_Chr6g0293041 RchiOBHm_Chr6g0293051 RchiOBHm_Chr6g0293061 RchiOBHm_Chr6g0293101 RchiOBHm_Chr6g0293111 RchiOBHm_Chr6g0293121 RchiOBHm_Chr6g0293131 RchiOBHm_Chr7g0223561
rosa_laevigata RLG00000011974 RLG00000015894
rosa_multiflora Rmu_co8377771.1_g000001 Rmu_co8466941.1_g000001 Rmu_sc0000811.1_g000022 Rmu_sc0000811.1_g000023 Rmu_sc0000838.1_g000008 Rmu_sc0001788.1_g000002 Rmu_sc0001788.1_g000003 Rmu_sc0001788.1_g000004 Rmu_sc0001836.1_g000024 Rmu_sc0001836.1_g000025 Rmu_sc0001903.1_g000013 Rmu_sc0002247.1_g000010 Rmu_sc0002247.1_g000011 Rmu_sc0002826.1_g000016 Rmu_sc0003629.1_g000020 Rmu_sc0003874.1_g000015 Rmu_sc0004481.1_g000017 Rmu_sc0005212.1_g000007 Rmu_sc0005710.1_g000001 Rmu_sc0005823.1_g000013 Rmu_sc0006405.1_g000009 Rmu_sc0006405.1_g000010 Rmu_sc0006801.1_g000010 Rmu_sc0008168.1_g000005 Rmu_sc0014786.1_g000001 Rmu_sc0032118.1_g000001 Rmu_sc0032118.1_g000002 Rmu_ssc0000042.1_g000006
rosa_roxburghii Rroxscaffold_2G00111260 Rroxscaffold_3G00235580 Rroxscaffold_4G00322490 Rroxscaffold_4G00324780 Rroxscaffold_4G00324950 Rroxscaffold_5G00333760 Rroxscaffold_7G00174460 Rroxscaffold_7G00174470 Rroxscaffold_7G00191990
rosa_rugosa Rorug01G0051700 Rorug01G0052100 Rorug01G0069100 Rorug01G0165700 Rorug01G0478600 Rorug02G0311200 Rorug03G0305800.1 Rorug03G0305900.1 Rorug03G0306000 Rorug06G0103900 Rorug06G0232100 Rorug06G0232200 Rorug06G0232300 Rorug06G0232400.1 Rorug06G0232500 Rorug06G0232700
rosa_samantha Rh1AG066700 Rh1AG067400 Rh1AG067500 Rh1AG086200 Rh1AG086300 Rh1BG055100 Rh1BG068800 Rh1CG068800 Rh1CG083700 Rh1CG083800 Rh2AG032500 Rh2BG031600 Rh2BG353500 Rh2CG032000 Rh2CG332100 Rh2DG032000 Rh2DG371500 Rh4AG009800 Rh4BG006800 Rh4CG010600 Rh5AG192200 Rh6AG214100 Rh6AG283700 Rh6AG344800 Rh6AG344900 Rh6AG345000 Rh6AG345100 Rh6AG345200 Rh6AG345300 Rh6BG218900 Rh6BG245300 Rh6BG352300 Rh6BG352400 Rh6BG352600 Rh6BG352700 Rh6BG352800 Rh6BG353400 Rh6DG211600 Rh6DG279100 Rh6DG344900 Rh6DG345000 Rh6DG345100 Rh6DG345200 Rh6DG345300 Rh6DG345900 Rh7BG351900
rosa_wichuraiana Rw1G005450 Rw1G005580 Rw1G006730 Rw2G002540 Rw4G000160 Rw6G018720 Rw6G030090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 80
AccIII TCCGGA 1 cut(s) 28
AclWI GGATC 2 cut(s) 20, 33
AcuI CTGAAG 2 cut(s) 56, 128
AgsI TTSAA 1 cut(s) 457
AjnI CCWGG 1 cut(s) 216
AluBI AGCT 5 cut(s) 34, 94, 251, 280, 491
AluI AGCT 5 cut(s) 34, 94, 251, 280, 491
Alw21I GWGCWC 3 cut(s) 96, 282, 359
Alw26I GTCTC 1 cut(s) 171
AlwI GGATC 2 cut(s) 20, 33
Aor13HI TCCGGA 1 cut(s) 28
AsuHPI GGTGA 2 cut(s) 166, 418
BamHI GGATCC 1 cut(s) 25
BanII GRGCYC 2 cut(s) 96, 282
Bbv12I GWGCWC 3 cut(s) 96, 282, 359
BccI CCATC 4 cut(s) 65, 311, 419, 425
BciT130I CCWGG 1 cut(s) 218
BcoDI GTCTC 1 cut(s) 171
Bme1390I CCNGG 1 cut(s) 218
BmiI GGNNCC 1 cut(s) 27
BmrFI CCNGG 1 cut(s) 218
BpuEI CTTGAG 2 cut(s) 80, 266
BsaJI CCNNGG 1 cut(s) 217
BsaWI WCCGGW 1 cut(s) 28
BseAI TCCGGA 1 cut(s) 28
BseBI CCWGG 1 cut(s) 218
BseDI CCNNGG 1 cut(s) 217
BseGI GGATG 1 cut(s) 430
BseRI GAGGAG 3 cut(s) 287, 290, 350
BsiHKAI GWGCWC 3 cut(s) 96, 282, 359
BsiSI CCGG 1 cut(s) 29
BsmAI GTCTC 1 cut(s) 171
BsmBI CGTCTC 1 cut(s) 171
Bsp1286I GDGCHC 3 cut(s) 96, 282, 359
Bsp13I TCCGGA 1 cut(s) 28
Bsp143I GATC 2 cut(s) 25, 186
BspEI TCCGGA 1 cut(s) 28
BspHI TCATGA 1 cut(s) 126
BspLI GGNNCC 1 cut(s) 27
BspPI GGATC 2 cut(s) 20, 33
BssECI CCNNGG 1 cut(s) 217
BssMI GATC 2 cut(s) 25, 186
Bst2UI CCWGG 1 cut(s) 218
Bst4CI ACNGT 1 cut(s) 68
BstDEI CTNAG 1 cut(s) 233
BstF5I GGATG 1 cut(s) 430
BstKTI GATC 2 cut(s) 28, 189
BstMAI GTCTC 1 cut(s) 171
BstMBI GATC 2 cut(s) 25, 186
BstNI CCWGG 1 cut(s) 218
BstSCI CCNGG 1 cut(s) 216
BstX2I RGATCY 1 cut(s) 25
BstYI RGATCY 1 cut(s) 25
BtsCI GGATG 1 cut(s) 430
BtsIMutI CAGTG 1 cut(s) 73
CciI TCATGA 1 cut(s) 126
CseI GACGC 1 cut(s) 176
CviAII CATG 1 cut(s) 127
CviJI RGCY 9 cut(s) 34, 94, 184, 251, 280, 290, 392, 481, 491
CviKI_1 RGCY 9 cut(s) 34, 94, 184, 251, 280, 290, 392, 481, 491
DdeI CTNAG 1 cut(s) 233
DpnI GATC 2 cut(s) 27, 188
DpnII GATC 2 cut(s) 25, 186
Ecl136II GAGCTC 2 cut(s) 94, 280
Eco24I GRGCYC 2 cut(s) 96, 282
Eco53kI GAGCTC 2 cut(s) 94, 280
Eco57I CTGAAG 2 cut(s) 56, 128
EcoICRI GAGCTC 2 cut(s) 94, 280
EcoRII CCWGG 1 cut(s) 216
EcoT38I GRGCYC 2 cut(s) 96, 282
Esp3I CGTCTC 1 cut(s) 171
FaeI CATG 1 cut(s) 130
FaiI YATR 4 cut(s) 80, 128, 230, 437
FatI CATG 1 cut(s) 126
FokI GGATG 1 cut(s) 437
FriOI GRGCYC 2 cut(s) 96, 282
HapII CCGG 1 cut(s) 29
HgaI GACGC 1 cut(s) 176
Hin1II CATG 1 cut(s) 130
HinfI GANTC 1 cut(s) 101
HpaII CCGG 1 cut(s) 29
HphI GGTGA 2 cut(s) 166, 418
Hpy166II GTNNAC 1 cut(s) 166
Hpy188I TCNGA 1 cut(s) 199
Hpy188III TCNNGA 3 cut(s) 29, 127, 457
Hpy8I GTNNAC 1 cut(s) 166
Hpy99I CGWCG 1 cut(s) 55
HpyAV CCTTC 1 cut(s) 406
HpyCH4III ACNGT 1 cut(s) 68
HpyCH4IV ACGT 1 cut(s) 53
HpyF3I CTNAG 1 cut(s) 233
HpySE526I ACGT 1 cut(s) 53
Hsp92II CATG 1 cut(s) 130
Kpn2I TCCGGA 1 cut(s) 28
Kzo9I GATC 2 cut(s) 25, 186
LmnI GCTCC 5 cut(s) 16, 31, 91, 265, 277
LpnPI CCDG 7 cut(s) 5, 42, 74, 103, 203, 230, 237
MaeII ACGT 1 cut(s) 53
MalI GATC 2 cut(s) 27, 188
MboI GATC 2 cut(s) 25, 186
MboII GAAGA 5 cut(s) 121, 169, 254, 257, 421
MflI RGATCY 1 cut(s) 25
MhlI GDGCHC 3 cut(s) 96, 282, 359
MluCI AATT 1 cut(s) 442
MlyI GAGTC 1 cut(s) 110
MroI TCCGGA 1 cut(s) 28
MseI TTAA 1 cut(s) 441
MslI CAYNNNNRTG 1 cut(s) 119
MspI CCGG 1 cut(s) 29
MspR9I CCNGG 1 cut(s) 218
MvaI CCWGG 1 cut(s) 218
NdeII GATC 2 cut(s) 25, 186
NlaIII CATG 1 cut(s) 130
NlaIV GGNNCC 1 cut(s) 27
PagI TCATGA 1 cut(s) 126
PcsI WCGNNNNNNNCGW 2 cut(s) 186, 195
PleI GAGTC 1 cut(s) 109
PpsI GAGTC 1 cut(s) 109
PsiI TTATAA 1 cut(s) 80
Psp124BI GAGCTC 2 cut(s) 96, 282
Psp6I CCWGG 1 cut(s) 216
PspGI CCWGG 1 cut(s) 216
PspN4I GGNNCC 1 cut(s) 27
PsuI RGATCY 1 cut(s) 25
RseI CAYNNNNRTG 1 cut(s) 119
SacI GAGCTC 2 cut(s) 96, 282
SaqAI TTAA 1 cut(s) 441
Sau3AI GATC 2 cut(s) 25, 186
SchI GAGTC 1 cut(s) 110
ScrFI CCNGG 1 cut(s) 218
SduI GDGCHC 3 cut(s) 96, 282, 359
SetI ASST 9 cut(s) 36, 56, 96, 253, 264, 282, 417, 475, 493
SmiMI CAYNNNNRTG 1 cut(s) 119
SmlI CTYRAG 2 cut(s) 95, 281
SmoI CTYRAG 2 cut(s) 95, 281
Sse9I AATT 1 cut(s) 442
SstI GAGCTC 2 cut(s) 96, 282
StyD4I CCNGG 1 cut(s) 216
TaaI ACNGT 1 cut(s) 68
TaiI ACGT 1 cut(s) 56
TaqI TCGA 2 cut(s) 57, 189
TasI AATT 1 cut(s) 442
Tru1I TTAA 1 cut(s) 441
Tru9I TTAA 1 cut(s) 441
TscAI CASTG 1 cut(s) 73
TspDTI ATGAA 3 cut(s) 115, 162, 482
TspGWI ACGGA 1 cut(s) 189
TspRI CASTG 1 cut(s) 73
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.