FvH4_1g14620

B3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0000593
Physical Location & Seq
Reverse (-)
8185258 .. 8188450
3193 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g14620.t1

Sequence Viewer

Length: 1362 bp
ATGGCAACAAGAAGAAACCCCTCTTTCTTCAAGGTTCTTATAGGGGATCACTTCTGTGACAAATTGAAGATCCCCAGAGCTTTTCTTAGGCATTTTGATGGGAATGTACCTGAACAATGCCAACTTAGAATCCTCACTCAAACTTGGCTTGTTGATATTGAAATGGATAATCACAAGTTCTATTTCCAAAATGGTTGGAAGGAATTTGTGCATGAGAATGGTTTAAAGGGTCGCGAGTTTCTAGTTTTTTTCTATGATGGAATTTCGGAGTTCTATGTCCACATATATGGGGAAAATGCCTGCAAAAAGGAGTTTGCAGAGGTCACTAGGAACTATGTTGCTAGCTTCAGAGAGATGGAAGAAGCTGATAGTGATGACACATCTGTTGAAATCTTGGATGACTTTCCACCCAGCTCAAGGAGAGCAGGGCTAGGGAACAAATCTTCGTTACCAAGTCATCAGACTCACAAGAAAGACGGTACAAGTTGGAGTAATAAAGCAGTAAATGGAGCAATGTATAAGAAATCAGGAACTACAAGTATAGATGGATTTCCGGCGGAACAATTTGTGGGAGGTACCTCTAGCTCAAAAAGACTCGTACAACCAGAATTGGAGGCTCTTGGGAGGATGCACATTGATGACGACGATGATGACAAAGAAGAGGAGAGCGAGGAGGATGAAACTGAAGCTGACAGTGAAAATGAAGATGAGGAAGAGGAAGAAGAAAGTGATGGAGATGAAGAAAAAAGTGATGATTCTATTGAAATTCTGTACGAGTTTCCACAATGCTCAAAAGCAAAAACAATGAGGAAATCATCAGGGCTTCACAAGGGAAAGATAAACAGTTCTAAAGCTAAACCACAAAGCAGCATAACAGGTGCTAGTGGCTCATCTAGCAATAAAAAAATCACAGTAATGAAACACAAGGGAACCGGAGACATTATAGCTCGTTGTAGAGCTAGTGATTTTATATCCAAATCTGACAAGCCTTCTTGTTCAATTGCTATGTCTCCTTCACATGTTCATGCAGCTTGGGTGCATTTTCCTTGTCTTTTTGCCGAGAGACATCTCAAAAAGCATTGTAGTTGCATCAACCTTCGGGTTAATACGAGAAGTTGGTGTATCAGTCTCTATCATGTCAAGAAATTTGTCAGATTACAAACTGGTTGGAGGCAATTTGCGCAGGCAAATAATCTGAAAGAGGGTGATGTGTGCGTCTTAGTGTTGATTGACAACATCAAATATGTATTTGATGTTACAATTTTCCGGGCCACAGAAGCTGCAAATTGCACCTTGCCAGGGGGAAGGCAAATTACCGAGGTTCAATTCAAGTCAAAGAAAGAAAAAGAGAAAGCTGGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

454

Amino Acids

51.3

Weight (kDa)

5.94

Isoelectric Point (pI)

55.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 9 - 97 9.9e-13 B3 DNA binding domain
B3 PF02362 336 - 423 1.3e-11 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1182
Acc65I GGTACC 1 cut(s) 575
AccB1I GGYRCC 1 cut(s) 575
AccII CGCG 1 cut(s) 234
AciI CCGC 1 cut(s) 557
AclWI GGATC 2 cut(s) 54, 64
AcsI RAATTY 4 cut(s) 203, 261, 765, 1145
AcuI CTGAAG 2 cut(s) 331, 705
AfaI GTAC 5 cut(s) 108, 481, 577, 600, 773
AfiI CCNNNNNNNGG 2 cut(s) 417, 1299
AflIII ACRYGT 1 cut(s) 1018
AgsI TTSAA 8 cut(s) 31, 67, 161, 389, 764, 999, 1325, 1330
AjnI CCWGG 1 cut(s) 1297
AleI CACNNNNGTG 1 cut(s) 54
Alw26I GTCTC 4 cut(s) 930, 1014, 1057, 1133
AlwI GGATC 2 cut(s) 54, 64
AlwNI CAGNNNCTG 1 cut(s) 1280
AoxI GGCC 1 cut(s) 1269
ApeKI GCWGC 3 cut(s) 867, 1028, 1280
ApoI RAATTY 4 cut(s) 203, 261, 765, 1145
Asp718I GGTACC 1 cut(s) 575
AspLEI GCGC 1 cut(s) 1183
AspS9I GGNCC 1 cut(s) 1269
AsuC2I CCSGG 1 cut(s) 1268
AsuHPI GGTGA 1 cut(s) 1217
AsuNHI GCTAGC 1 cut(s) 341
BanI GGYRCC 1 cut(s) 575
BarI GAAGNNNNNNTAC 2 cut(s) 1297, 1329
BbvI GCAGC 3 cut(s) 879, 1040, 1267
BccI CCATC 5 cut(s) 92, 251, 349, 539, 725
BciT130I CCWGG 1 cut(s) 1299
BcnI CCSGG 1 cut(s) 1268
BcoDI GTCTC 4 cut(s) 930, 1014, 1057, 1133
BfaI CTAG 8 cut(s) 242, 327, 342, 431, 582, 882, 894, 960
BisI GCNGC 3 cut(s) 868, 1029, 1281
BlsI GCNGC 3 cut(s) 869, 1030, 1282
Bme1390I CCNGG 2 cut(s) 1268, 1299
BmgT120I GGNCC 1 cut(s) 1269
BmiI GGNNCC 2 cut(s) 577, 931
BmrFI CCNGG 2 cut(s) 1268, 1299
BmsI GCATC 2 cut(s) 618, 1098
BmtI GCTAGC 1 cut(s) 345
BpuEI CTTGAG 1 cut(s) 400
BpuMI CCSGG 1 cut(s) 1268
BsaJI CCNNGG 2 cut(s) 1298, 1317
BsaWI WCCGGW 1 cut(s) 932
Bsc4I CCNNNNNNNGG 2 cut(s) 417, 1299
Bse1I ACTGG 1 cut(s) 1168
Bse3DI GCAATG 1 cut(s) 519
BseBI CCWGG 1 cut(s) 1299
BseDI CCNNGG 2 cut(s) 1298, 1317
BseGI GGATG 3 cut(s) 403, 633, 682
BseLI CCNNNNNNNGG 2 cut(s) 417, 1299
BseMI GCAATG 1 cut(s) 519
BseNI ACTGG 1 cut(s) 1168
BseRI GAGGAG 2 cut(s) 677, 686
BseXI GCAGC 3 cut(s) 879, 1040, 1267
BseYI CCCAGC 1 cut(s) 410
Bsh1236I CGCG 1 cut(s) 234
BshFI GGCC 1 cut(s) 1271
BshNI GGYRCC 1 cut(s) 575
BsiSI CCGG 3 cut(s) 554, 933, 1267
BslI CCNNNNNNNGG 2 cut(s) 417, 1299
BsmAI GTCTC 4 cut(s) 930, 1014, 1057, 1133
BsnI GGCC 1 cut(s) 1271
Bsp143I GATC 2 cut(s) 46, 69
Bsp68I TCGCGA 1 cut(s) 234
BspACI CCGC 1 cut(s) 557
BspANI GGCC 1 cut(s) 1271
BspFNI CGCG 1 cut(s) 234
BspLI GGNNCC 2 cut(s) 577, 931
BspOI GCTAGC 1 cut(s) 345
BspPI GGATC 2 cut(s) 54, 64
BspT107I GGYRCC 1 cut(s) 575
BsrDI GCAATG 1 cut(s) 519
BsrI ACTGG 1 cut(s) 1168
BssECI CCNNGG 2 cut(s) 1298, 1317
BssMI GATC 2 cut(s) 46, 69
Bst2UI CCWGG 1 cut(s) 1299
Bst4CI ACNGT 4 cut(s) 479, 695, 845, 913
Bst6I CTCTTC 2 cut(s) 654, 708
BstC8I GCNNGC 3 cut(s) 301, 343, 1185
BstDEI CTNAG 3 cut(s) 86, 125, 1219
BstF5I GGATG 3 cut(s) 403, 633, 682
BstFNI CGCG 1 cut(s) 234
BstHHI GCGC 1 cut(s) 1183
BstKTI GATC 2 cut(s) 49, 72
BstMAI GTCTC 4 cut(s) 930, 1014, 1057, 1133
BstMBI GATC 2 cut(s) 46, 69
BstMWI GCNNNNNNNGC 3 cut(s) 894, 1180, 1277
BstNI CCWGG 1 cut(s) 1299
BstNSI RCATGY 1 cut(s) 1022
BstSCI CCNGG 2 cut(s) 1266, 1297
BstUI CGCG 1 cut(s) 234
BstV1I GCAGC 3 cut(s) 879, 1040, 1267
BstX2I RGATCY 1 cut(s) 69
BstXI CCANNNNNNTGG 1 cut(s) 287
BstYI RGATCY 1 cut(s) 69
BsuRI GGCC 1 cut(s) 1271
BtsCI GGATG 3 cut(s) 403, 633, 682
BtsIMutI CAGTG 1 cut(s) 700
BtuMI TCGCGA 1 cut(s) 234
Cac8I GCNNGC 3 cut(s) 301, 343, 1185
CaiI CAGNNNCTG 1 cut(s) 1280
CfoI GCGC 1 cut(s) 1183
Cfr13I GGNCC 1 cut(s) 1269
CseI GACGC 1 cut(s) 1204
Csp6I GTAC 5 cut(s) 107, 480, 576, 599, 772
CviAII CATG 4 cut(s) 212, 1019, 1025, 1136
CviQI GTAC 5 cut(s) 107, 480, 576, 599, 772
DdeI CTNAG 3 cut(s) 86, 125, 1219
DpnI GATC 2 cut(s) 48, 71
DpnII GATC 2 cut(s) 46, 69
DraI TTTAAA 1 cut(s) 225
Eam1104I CTCTTC 2 cut(s) 654, 708
EarI CTCTTC 2 cut(s) 654, 708
EciI GGCGGA 1 cut(s) 572
Eco57I CTGAAG 2 cut(s) 331, 705
EcoRII CCWGG 1 cut(s) 1297
FaeI CATG 4 cut(s) 215, 1022, 1028, 1139
FatI CATG 4 cut(s) 211, 1018, 1024, 1135
Fnu4HI GCNGC 3 cut(s) 868, 1029, 1281
FokI GGATG 3 cut(s) 410, 640, 689
Fsp4HI GCNGC 3 cut(s) 868, 1029, 1281
FspBI CTAG 8 cut(s) 242, 327, 342, 431, 582, 882, 894, 960
FspI TGCGCA 1 cut(s) 1182
GlaI GCGC 1 cut(s) 1182
GluI GCNGC 3 cut(s) 868, 1029, 1281
GsaI CCCAGC 1 cut(s) 414
HaeIII GGCC 1 cut(s) 1271
HapII CCGG 3 cut(s) 554, 933, 1267
HgaI GACGC 1 cut(s) 1204
HhaI GCGC 1 cut(s) 1183
Hin1II CATG 4 cut(s) 215, 1022, 1028, 1139
Hin6I GCGC 1 cut(s) 1181
HinP1I GCGC 1 cut(s) 1181
HinfI GANTC 4 cut(s) 129, 463, 594, 755
HpaII CCGG 3 cut(s) 554, 933, 1267
HphI GGTGA 1 cut(s) 1217
Hpy166II GTNNAC 1 cut(s) 280
Hpy188I TCNGA 6 cut(s) 268, 350, 462, 982, 1154, 1197
Hpy188III TCNNGA 3 cut(s) 233, 528, 1141
Hpy8I GTNNAC 1 cut(s) 280
Hpy99I CGWCG 1 cut(s) 647
HpyAV CCTTC 5 cut(s) 193, 999, 1023, 1106, 1299
HpyCH4III ACNGT 4 cut(s) 479, 695, 845, 913
HpyCH4V TGCA 9 cut(s) 211, 303, 317, 631, 1028, 1039, 1089, 1283, 1290
HpyF10VI GCNNNNNNNGC 3 cut(s) 894, 1180, 1277
HpyF3I CTNAG 3 cut(s) 86, 125, 1219
Hsp92II CATG 4 cut(s) 215, 1022, 1028, 1139
HspAI GCGC 1 cut(s) 1181
KpnI GGTACC 1 cut(s) 579
Kzo9I GATC 2 cut(s) 46, 69
LmnI GCTCC 1 cut(s) 509
Lsp1109I GCAGC 3 cut(s) 879, 1040, 1267
LweI GCATC 2 cut(s) 618, 1098
MaeI CTAG 8 cut(s) 242, 327, 342, 431, 582, 882, 894, 960
MaeIII GTNAC 4 cut(s) 56, 322, 447, 1255
MalI GATC 2 cut(s) 48, 71
MboI GATC 2 cut(s) 46, 69
MfeI CAATTG 1 cut(s) 999
MflI RGATCY 1 cut(s) 69
MlyI GAGTC 2 cut(s) 457, 588
MmeI TCCRAC 3 cut(s) 176, 467, 1148
MseI TTAA 2 cut(s) 224, 1104
MslI CAYNNNNRTG 7 cut(s) 54, 96, 216, 285, 636, 914, 1023
MspI CCGG 3 cut(s) 554, 933, 1267
MspR9I CCNGG 2 cut(s) 1268, 1299
MunI CAATTG 1 cut(s) 999
MvaI CCWGG 1 cut(s) 1299
MvnI CGCG 1 cut(s) 234
MwoI GCNNNNNNNGC 3 cut(s) 894, 1180, 1277
NciI CCSGG 1 cut(s) 1268
NdeII GATC 2 cut(s) 46, 69
NheI GCTAGC 1 cut(s) 341
NlaIII CATG 4 cut(s) 215, 1022, 1028, 1139
NlaIV GGNNCC 2 cut(s) 577, 931
NmeAIII GCCGAG 1 cut(s) 1084
NmuCI GTSAC 2 cut(s) 56, 322
NruI TCGCGA 1 cut(s) 234
NsbI TGCGCA 1 cut(s) 1182
NspI RCATGY 1 cut(s) 1022
OliI CACNNNNGTG 1 cut(s) 54
PciI ACATGT 1 cut(s) 1018
PfeI GAWTC 2 cut(s) 129, 755
PkrI GCNGC 3 cut(s) 869, 1030, 1282
PleI GAGTC 2 cut(s) 457, 588
PpsI GAGTC 2 cut(s) 457, 588
PscI ACATGT 1 cut(s) 1018
Psp6I CCWGG 1 cut(s) 1297
PspFI CCCAGC 1 cut(s) 410
PspGI CCWGG 1 cut(s) 1297
PspN4I GGNNCC 2 cut(s) 577, 931
PspPI GGNCC 1 cut(s) 1269
PstNI CAGNNNCTG 1 cut(s) 1280
PsuI RGATCY 1 cut(s) 69
RruI TCGCGA 1 cut(s) 234
RsaI GTAC 5 cut(s) 108, 481, 577, 600, 773
RsaNI GTAC 5 cut(s) 107, 480, 576, 599, 772
RseI CAYNNNNRTG 7 cut(s) 54, 96, 216, 285, 636, 914, 1023
SaqAI TTAA 2 cut(s) 224, 1104
SatI GCNGC 3 cut(s) 868, 1029, 1281
Sau3AI GATC 2 cut(s) 46, 69
Sau96I GGNCC 1 cut(s) 1269
SchI GAGTC 2 cut(s) 457, 588
ScrFI CCNGG 2 cut(s) 1268, 1299
SfaNI GCATC 2 cut(s) 618, 1098
SmiMI CAYNNNNRTG 7 cut(s) 54, 96, 216, 285, 636, 914, 1023
SmlI CTYRAG 1 cut(s) 415
SmoI CTYRAG 1 cut(s) 415
SsiI CCGC 1 cut(s) 557
SspMI CTAG 8 cut(s) 242, 327, 342, 431, 582, 882, 894, 960
StyD4I CCNGG 2 cut(s) 1266, 1297
TaaI ACNGT 4 cut(s) 479, 695, 845, 913
TfiI GAWTC 2 cut(s) 129, 755
Tru1I TTAA 2 cut(s) 224, 1104
Tru9I TTAA 2 cut(s) 224, 1104
TscAI CASTG 1 cut(s) 700
TseFI GTSAC 2 cut(s) 56, 322
TseI GCWGC 3 cut(s) 867, 1028, 1280
Tsp45I GTSAC 2 cut(s) 56, 322
TspDTI ATGAA 5 cut(s) 693, 717, 753, 932, 1013
TspRI CASTG 1 cut(s) 700
XapI RAATTY 4 cut(s) 203, 261, 765, 1145
XceI RCATGY 1 cut(s) 1022
XspI CTAG 8 cut(s) 242, 327, 342, 431, 582, 882, 894, 960
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.