MD15G1273900.v1.1

B3 DNA binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0000593
Physical Location & Seq
Reverse (-)
24032620 .. 24035055
2436 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1273900.v1.1.491

Sequence Viewer

Length: 1074 bp
ATGCTCAACACACACCAAACCAAGGACGGTTCTGAGTCTTTGACCATTTCTCCGTTCAGAAGAGAAGCGCTGAAGAGGAAGAAGAAAATGGTGATGGCAAGATCAAATGCAGTAAGAGCACCCACTTTTTTCAAGGTTCTTGTAGATGACTTCTCCACCCAGTTGCAAATCCCGCTGGCTTTTCTCGTGCATTTTGATGGAAAGGTACCTCAAGAGACCCATCTTCAGACCCCAGGTGGAACTTGGCCTGTTAGTCTGGAAAAACGTAATGACAGGTTCTTTTTCCAAAAGGGTTGGAAGGAATTTGTGCAGGACAATGGTTTCCAGCTTTGTGAGTTCCTAGTTTTTCGATATTATGAAAAATTGGGATTCTATGTTGACATATACGGAAGAAATGGATGCAAAAAGGAGTTTGTGATGGTTATTAGGAAGAGGGACAGACCTCATGAGGAGGCACATGGCAATGCAGTTCACAGAAAGACACCAAGAAATCATGGAAAGCAGAATGTAGATAACCAAGGTCAAAAAGGCTTGGCTTATGGAACTGGGACTAGTCGTGCGACTCATGCCAATATTCAAATCAAAGATGAAGTAACTGATGATAGAGAATATGAATTATCGATGCATCCAAGGGCTACAAGAACTGAGAAAGCTGTTGCTCTTCAGAAAGGCAATGATTTTGAATATCGAAACCCTTTTTTCAAGGTTCCACTGCGGTTCTCATATACCCATTATGGTTATCTGGCTTTGCCAGTTATGTTTGTCAAGGCACATCTCACTGTGAGGCCTTCTGATGCTACCCTTCGGGTTTCTGATGGAACAACCTGGCCTGTTAAAGTTGGATTTGATGTAGCTGGACACTGCAGATTCCTTAGTGGTTGGAGGCTATTTGTGAACGACAATGGTTTAGAAATTGGTGATATATGTGTCTTTGTCTTGATTAACAAGATTGAGTTTTTGTTTGAAGTTGTTCTTCACCGCAAGATAGGAGCTGTGAGTCCTACCTTGTCACCAGTGGAAGACAAACTGCGGCGGAGGATCAATTCGAGTTACGAAATCAAGATAGAAGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

358

Amino Acids

41.23

Weight (kDa)

9.59

Isoelectric Point (pI)

37.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 43 - 131 3.9e-13 B3 DNA binding domain
B3 PF02362 233 - 326 1.5e-13 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 205
AccB1I GGYRCC 1 cut(s) 205
AciI CCGC 5 cut(s) 173, 715, 979, 1030, 1033
AclWI GGATC 1 cut(s) 1046
AcsI RAATTY 1 cut(s) 302
AcuI CTGAAG 3 cut(s) 92, 209, 647
AfaI GTAC 1 cut(s) 207
AfeI AGCGCT 1 cut(s) 69
AfiI CCNNNNNNNGG 1 cut(s) 22
AgsI TTSAA 5 cut(s) 133, 578, 683, 703, 965
AhlI ACTAGT 1 cut(s) 551
AjnI CCWGG 2 cut(s) 232, 824
AluBI AGCT 4 cut(s) 328, 653, 854, 992
AluI AGCT 4 cut(s) 328, 653, 854, 992
Alw21I GWGCWC 1 cut(s) 121
Alw26I GTCTC 1 cut(s) 209
AlwI GGATC 1 cut(s) 1046
Aor51HI AGCGCT 1 cut(s) 69
AoxI GGCC 3 cut(s) 245, 785, 827
ApoI RAATTY 1 cut(s) 302
Asp700I GAANNNNTTC 1 cut(s) 969
Asp718I GGTACC 1 cut(s) 205
AspLEI GCGC 1 cut(s) 70
AsuHPI GGTGA 4 cut(s) 103, 929, 968, 1002
BanI GGYRCC 1 cut(s) 205
BauI CACGAG 1 cut(s) 185
BbsI GAAGAC 1 cut(s) 1026
Bbv12I GWGCWC 1 cut(s) 121
BccI CCATC 5 cut(s) 88, 191, 228, 412, 809
BciT130I CCWGG 2 cut(s) 234, 826
BcoDI GTCTC 1 cut(s) 209
BcuI ACTAGT 1 cut(s) 551
BfaI CTAG 2 cut(s) 341, 552
BfmI CTRYAG 1 cut(s) 862
BfoI RGCGCY 1 cut(s) 71
BisI GCNGC 1 cut(s) 1031
BlsI GCNGC 1 cut(s) 1032
Bme1390I CCNGG 2 cut(s) 234, 826
BmiI GGNNCC 2 cut(s) 207, 708
BmrFI CCNGG 2 cut(s) 234, 826
BmrI ACTGGG 2 cut(s) 154, 555
BmsI GCATC 4 cut(s) 389, 612, 634, 784
BmuI ACTGGG 2 cut(s) 154, 555
BpiI GAAGAC 1 cut(s) 1026
BpuEI CTTGAG 1 cut(s) 195
Bsa29I ATCGAT 1 cut(s) 620
BsaI GGTCTC 1 cut(s) 209
BsaJI CCNNGG 4 cut(s) 21, 232, 517, 629
BsaXI ACNNNNNCTCC 2 cut(s) 34, 64
Bsc4I CCNNNNNNNGG 1 cut(s) 22
Bse1I ACTGG 4 cut(s) 160, 550, 752, 1013
Bse3DI GCAATG 2 cut(s) 469, 679
BseBI CCWGG 2 cut(s) 234, 826
BseCI ATCGAT 1 cut(s) 620
BseDI CCNNGG 4 cut(s) 21, 232, 517, 629
BseGI GGATG 2 cut(s) 404, 625
BseLI CCNNNNNNNGG 1 cut(s) 22
BseMI GCAATG 2 cut(s) 469, 679
BseMII CTCAG 2 cut(s) 24, 636
BseNI ACTGG 4 cut(s) 160, 550, 752, 1013
BseRI GAGGAG 1 cut(s) 464
BsgI GTGCAG 1 cut(s) 329
BshFI GGCC 3 cut(s) 247, 787, 829
BshNI GGYRCC 1 cut(s) 205
BshVI ATCGAT 1 cut(s) 620
BsiHKAI GWGCWC 1 cut(s) 121
BslFI GGGAC 2 cut(s) 449, 562
BslI CCNNNNNNNGG 1 cut(s) 22
BsmAI GTCTC 1 cut(s) 209
BsmFI GGGAC 2 cut(s) 449, 562
BsnI GGCC 3 cut(s) 247, 787, 829
Bso31I GGTCTC 1 cut(s) 209
Bsp1286I GDGCHC 1 cut(s) 121
Bsp143I GATC 2 cut(s) 101, 1038
BspACI CCGC 5 cut(s) 173, 715, 979, 1030, 1033
BspANI GGCC 3 cut(s) 247, 787, 829
BspCNI CTCAG 2 cut(s) 25, 637
BspDI ATCGAT 1 cut(s) 620
BspHI TCATGA 1 cut(s) 445
BspLI GGNNCC 2 cut(s) 207, 708
BspMAI CTGCAG 1 cut(s) 866
BspPI GGATC 1 cut(s) 1046
BspQI GCTCTTC 1 cut(s) 666
BspT107I GGYRCC 1 cut(s) 205
BspTNI GGTCTC 1 cut(s) 209
BsrDI GCAATG 2 cut(s) 469, 679
BsrI ACTGG 4 cut(s) 160, 550, 752, 1013
BssECI CCNNGG 4 cut(s) 21, 232, 517, 629
BssMI GATC 2 cut(s) 101, 1038
BssSI CACGAG 1 cut(s) 185
BssT1I CCWWGG 3 cut(s) 21, 517, 629
Bst2BI CACGAG 1 cut(s) 185
Bst2UI CCWGG 2 cut(s) 234, 826
Bst4CI ACNGT 2 cut(s) 29, 781
Bst6I CTCTTC 4 cut(s) 55, 68, 425, 666
BstC8I GCNNGC 1 cut(s) 177
BstDEI CTNAG 3 cut(s) 33, 645, 872
BstF5I GGATG 2 cut(s) 404, 625
BstH2I RGCGCY 1 cut(s) 71
BstHHI GCGC 1 cut(s) 70
BstKTI GATC 2 cut(s) 104, 1041
BstMAI GTCTC 1 cut(s) 209
BstMBI GATC 2 cut(s) 101, 1038
BstMWI GCNNNNNNNGC 3 cut(s) 116, 172, 566
BstNI CCWGG 2 cut(s) 234, 826
BstSCI CCNGG 2 cut(s) 232, 824
BstSFI CTRYAG 1 cut(s) 862
BstV2I GAAGAC 1 cut(s) 1026
Bsu15I ATCGAT 1 cut(s) 620
BsuRI GGCC 3 cut(s) 247, 787, 829
BsuTUI ATCGAT 1 cut(s) 620
BtsCI GGATG 2 cut(s) 404, 625
BtsI GCAGTG 2 cut(s) 710, 859
BtsIMutI CAGTG 4 cut(s) 710, 777, 859, 1020
Cac8I GCNNGC 1 cut(s) 177
CciI TCATGA 1 cut(s) 445
CfoI GCGC 1 cut(s) 70
ClaI ATCGAT 1 cut(s) 620
Csp6I GTAC 1 cut(s) 206
CviAII CATG 4 cut(s) 446, 458, 494, 566
CviQI GTAC 1 cut(s) 206
DdeI CTNAG 3 cut(s) 33, 645, 872
DpnI GATC 2 cut(s) 103, 1040
DpnII GATC 2 cut(s) 101, 1038
Eam1104I CTCTTC 4 cut(s) 55, 68, 425, 666
EarI CTCTTC 4 cut(s) 55, 68, 425, 666
EciI GGCGGA 1 cut(s) 1048
Eco130I CCWWGG 3 cut(s) 21, 517, 629
Eco147I AGGCCT 1 cut(s) 787
Eco31I GGTCTC 1 cut(s) 209
Eco47III AGCGCT 1 cut(s) 69
Eco57I CTGAAG 3 cut(s) 92, 209, 647
EcoRII CCWGG 2 cut(s) 232, 824
EcoT14I CCWWGG 3 cut(s) 21, 517, 629
EcoT22I ATGCAT 1 cut(s) 627
ErhI CCWWGG 3 cut(s) 21, 517, 629
FaeI CATG 4 cut(s) 449, 461, 497, 569
FalI AAGNNNNNCTT 2 cut(s) 957, 989
FaqI GGGAC 2 cut(s) 449, 562
FatI CATG 4 cut(s) 445, 457, 493, 565
FauI CCCGC 1 cut(s) 180
Fnu4HI GCNGC 1 cut(s) 1031
FokI GGATG 2 cut(s) 411, 612
Fsp4HI GCNGC 1 cut(s) 1031
FspBI CTAG 2 cut(s) 341, 552
GlaI GCGC 1 cut(s) 69
GluI GCNGC 1 cut(s) 1031
HaeII RGCGCY 1 cut(s) 71
HaeIII GGCC 3 cut(s) 247, 787, 829
HhaI GCGC 1 cut(s) 70
Hin1II CATG 4 cut(s) 449, 461, 497, 569
Hin6I GCGC 1 cut(s) 68
HinP1I GCGC 1 cut(s) 68
HincII GTYRAC 1 cut(s) 379
HindII GTYRAC 1 cut(s) 379
HinfI GANTC 5 cut(s) 35, 369, 562, 867, 997
HphI GGTGA 4 cut(s) 103, 929, 968, 1002
Hpy166II GTNNAC 3 cut(s) 379, 472, 895
Hpy188I TCNGA 6 cut(s) 34, 59, 228, 666, 793, 814
Hpy188III TCNNGA 5 cut(s) 212, 257, 446, 937, 1060
Hpy8I GTNNAC 3 cut(s) 379, 472, 895
HpyAV CCTTC 3 cut(s) 292, 798, 812
HpyCH4III ACNGT 2 cut(s) 29, 781
HpyCH4IV ACGT 1 cut(s) 265
HpyCH4V TGCA 8 cut(s) 110, 166, 190, 310, 402, 467, 625, 864
HpyF10VI GCNNNNNNNGC 3 cut(s) 116, 172, 566
HpyF3I CTNAG 3 cut(s) 33, 645, 872
HpySE526I ACGT 1 cut(s) 265
Hsp92II CATG 4 cut(s) 449, 461, 497, 569
HspAI GCGC 1 cut(s) 68
KpnI GGTACC 1 cut(s) 209
Kzo9I GATC 2 cut(s) 101, 1038
LguI GCTCTTC 1 cut(s) 666
LmnI GCTCC 1 cut(s) 989
LweI GCATC 4 cut(s) 389, 612, 634, 784
MaeI CTAG 2 cut(s) 341, 552
MaeII ACGT 1 cut(s) 265
MaeIII GTNAC 3 cut(s) 592, 1008, 1049
MalI GATC 2 cut(s) 103, 1040
MboI GATC 2 cut(s) 101, 1038
MhlI GDGCHC 1 cut(s) 121
MluCI AATT 5 cut(s) 302, 362, 614, 912, 1042
MlyI GAGTC 3 cut(s) 44, 556, 1006
MmeI TCCRAC 3 cut(s) 275, 820, 860
MnlI CCTC 9 cut(s) 69, 219, 426, 442, 445, 453, 777, 876, 1029
Mph1103I ATGCAT 1 cut(s) 627
MroXI GAANNNNTTC 1 cut(s) 969
MseI TTAA 2 cut(s) 834, 942
MslI CAYNNNNRTG 2 cut(s) 195, 462
MspA1I CMGCKG 1 cut(s) 175
MspR9I CCNGG 2 cut(s) 234, 826
MvaI CCWGG 2 cut(s) 234, 826
MwoI GCNNNNNNNGC 3 cut(s) 116, 172, 566
NdeII GATC 2 cut(s) 101, 1038
NlaIII CATG 4 cut(s) 449, 461, 497, 569
NlaIV GGNNCC 2 cut(s) 207, 708
NmuCI GTSAC 1 cut(s) 1008
NsiI ATGCAT 1 cut(s) 627
PagI TCATGA 1 cut(s) 445
PceI AGGCCT 1 cut(s) 787
PciSI GCTCTTC 1 cut(s) 666
PdmI GAANNNNTTC 1 cut(s) 969
PfeI GAWTC 2 cut(s) 369, 867
PkrI GCNGC 1 cut(s) 1032
PleI GAGTC 3 cut(s) 43, 556, 1005
PpsI GAGTC 3 cut(s) 43, 556, 1005
Psp6I CCWGG 2 cut(s) 232, 824
PspGI CCWGG 2 cut(s) 232, 824
PspN4I GGNNCC 2 cut(s) 207, 708
PstI CTGCAG 1 cut(s) 866
RsaI GTAC 1 cut(s) 207
RsaNI GTAC 1 cut(s) 206
RseI CAYNNNNRTG 2 cut(s) 195, 462
SapI GCTCTTC 1 cut(s) 666
SaqAI TTAA 2 cut(s) 834, 942
SatI GCNGC 1 cut(s) 1031
Sau3AI GATC 2 cut(s) 101, 1038
SchI GAGTC 3 cut(s) 44, 556, 1006
ScrFI CCNGG 2 cut(s) 234, 826
SduI GDGCHC 1 cut(s) 121
SfaNI GCATC 4 cut(s) 389, 612, 634, 784
SfcI CTRYAG 1 cut(s) 862
SmiMI CAYNNNNRTG 2 cut(s) 195, 462
SmlI CTYRAG 1 cut(s) 210
SmoI CTYRAG 1 cut(s) 210
SpeI ACTAGT 1 cut(s) 551
Sse9I AATT 5 cut(s) 302, 362, 614, 912, 1042
SseBI AGGCCT 1 cut(s) 787
SsiI CCGC 5 cut(s) 173, 715, 979, 1030, 1033
SspI AATATT 1 cut(s) 574
SspMI CTAG 2 cut(s) 341, 552
StuI AGGCCT 1 cut(s) 787
StyD4I CCNGG 2 cut(s) 232, 824
StyI CCWWGG 3 cut(s) 21, 517, 629
TaaI ACNGT 2 cut(s) 29, 781
TaiI ACGT 1 cut(s) 268
TaqI TCGA 4 cut(s) 349, 620, 688, 1046
TasI AATT 5 cut(s) 302, 362, 614, 912, 1042
TauI GCSGC 1 cut(s) 1033
TfiI GAWTC 2 cut(s) 369, 867
Tru1I TTAA 2 cut(s) 834, 942
Tru9I TTAA 2 cut(s) 834, 942
TscAI CASTG 4 cut(s) 717, 784, 866, 1020
TseFI GTSAC 1 cut(s) 1008
Tsp45I GTSAC 1 cut(s) 1008
TspDTI ATGAA 3 cut(s) 372, 603, 627
TspGWI ACGGA 2 cut(s) 42, 402
TspRI CASTG 4 cut(s) 717, 784, 866, 1020
XapI RAATTY 1 cut(s) 302
XcmI CCANNNNNNNNNTGG 1 cut(s) 240
XmnI GAANNNNTTC 1 cut(s) 969
XspI CTAG 2 cut(s) 341, 552
Zsp2I ATGCAT 1 cut(s) 627
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.