pycom15g23740

B3 DNA binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0000593
Physical Location & Seq
Reverse (-)
17891789 .. 17893845
2057 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g23740.1

Sequence Viewer

Length: 1053 bp
ATGCTCAACACACACCAAACCAAGGTCAGTTCTGAGTCTTTGACCATTTCTCCGTTCAGAAGAGAAACGCTGAAGAGGAAGAAGAAAATGATGATGGCAAGATCAAATGCAGTAAGAGCACCCACCTTTTTCAAGGTTCTTGTAGATGACTTCTCCACCCAGTTGCAAATCCCGCTGGCTTTTCTCTTGCATTTTGATGGAAAGGTACCTCAAGAGACCCATCTTCAGACCCCAGGTGGAACTTGGCCTGTTAGTCTGGAAAAACGTAATGACAGGCTCTTTTTCCAAAAGGGTTGGAAGGAATTTGTGCAGGACAATGGTTTCCAGCTTTGTGAGTTCCTAGTTTTTCGATATTATGAAAAATTGGGATTCTATGTTGACATATATGGAAGAAATGGATGCAAAAAGGAGTTTGTGGCGGTTATTAGGAAGAGGGACAGACCTCATGAGGAGGCACATGGCAATGCAGTTCACAGAAAGACACCAAGAAATCATGGAAAGCAGAATGTAGATAACCAAGGCCGAAAGGGCTTGGCTCATGGAACTGGGACTAGTCGTGCGACTCATGCCAATATTCAAATCAAAGATGAAGTAACTAATGATAGAGAATATGAATTATCGATGCATCGAAGGGCTACAAGAACTGAGAATGCTGTTGCTCTTCAGAGAGGCAATGGTTTTGAATATCGAAACCCTTTTTTCAAGGTTCCACTGCGGTTCTCATATACCCATTATGGTTATCTGGCTTTGCCAGGTATGTTTGTCAAGGCACATCTCACTGAGAGGCCTTCTGATGCTACCCTTCGGGTTTCTGATGGGAGAATTTGGCCTGTTAAAGTTGGATTTGATGTAGCTGGACACTGCAGATTCCTTAGCGGTTGGAGTCTATTTGTGAAAGACAATGGTTTAGAAATTGGTGATATATGTGTCTTTGTGTTGATTAACAAGATTGAGTTTTTGTTTGAAGTTGTTCTTCACCGCAAGATAGGAGCTGTGAGTCCTACCTTGTCACCAGTTTTGTGGAAGACAAACTGCAGCGGAGGATCAATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

351

Amino Acids

40.16

Weight (kDa)

9.82

Isoelectric Point (pI)

34.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 43 - 131 1.6e-12 B3 DNA binding domain
B3 PF02362 233 - 326 1.3e-13 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 205
AccB1I GGYRCC 1 cut(s) 205
AciI CCGC 6 cut(s) 173, 419, 715, 876, 979, 1038
AclWI GGATC 1 cut(s) 1051
AcsI RAATTY 2 cut(s) 302, 822
AcuI CTGAAG 3 cut(s) 92, 209, 647
AfaI GTAC 1 cut(s) 207
AfiI CCNNNNNNNGG 1 cut(s) 22
AgsI TTSAA 5 cut(s) 133, 578, 683, 703, 965
AhlI ACTAGT 1 cut(s) 551
AjnI CCWGG 2 cut(s) 232, 751
AluBI AGCT 3 cut(s) 328, 854, 992
AluI AGCT 3 cut(s) 328, 854, 992
Alw21I GWGCWC 1 cut(s) 121
Alw26I GTCTC 1 cut(s) 209
AlwI GGATC 1 cut(s) 1051
AoxI GGCC 4 cut(s) 245, 520, 785, 827
ApeKI GCWGC 1 cut(s) 1035
ApoI RAATTY 2 cut(s) 302, 822
Asp700I GAANNNNTTC 1 cut(s) 969
Asp718I GGTACC 1 cut(s) 205
AsuHPI GGTGA 3 cut(s) 929, 968, 1002
BanI GGYRCC 1 cut(s) 205
BbsI GAAGAC 1 cut(s) 1031
Bbv12I GWGCWC 1 cut(s) 121
BbvI GCAGC 1 cut(s) 1047
BccI CCATC 4 cut(s) 88, 191, 228, 809
BciT130I CCWGG 2 cut(s) 234, 753
BcoDI GTCTC 1 cut(s) 209
BcuI ACTAGT 1 cut(s) 551
BfaI CTAG 2 cut(s) 341, 552
BfmI CTRYAG 2 cut(s) 862, 1033
BglI GCCNNNNNGGC 1 cut(s) 528
BisI GCNGC 1 cut(s) 1036
BlsI GCNGC 1 cut(s) 1037
Bme1390I CCNGG 2 cut(s) 234, 753
BmiI GGNNCC 2 cut(s) 207, 708
BmrFI CCNGG 2 cut(s) 234, 753
BmrI ACTGGG 2 cut(s) 154, 555
BmsI GCATC 4 cut(s) 389, 612, 634, 784
BmuI ACTGGG 2 cut(s) 154, 555
BpiI GAAGAC 1 cut(s) 1031
Bpu10I CCTNAGC 1 cut(s) 872
BpuEI CTTGAG 1 cut(s) 195
Bsa29I ATCGAT 1 cut(s) 620
BsaI GGTCTC 1 cut(s) 209
BsaJI CCNNGG 3 cut(s) 21, 232, 517
BsaXI ACNNNNNCTCC 2 cut(s) 34, 64
Bsc4I CCNNNNNNNGG 1 cut(s) 22
Bse1I ACTGG 3 cut(s) 160, 550, 1013
Bse3DI GCAATG 2 cut(s) 469, 679
BseBI CCWGG 2 cut(s) 234, 753
BseCI ATCGAT 1 cut(s) 620
BseDI CCNNGG 3 cut(s) 21, 232, 517
BseGI GGATG 1 cut(s) 404
BseLI CCNNNNNNNGG 1 cut(s) 22
BseMI GCAATG 2 cut(s) 469, 679
BseMII CTCAG 3 cut(s) 24, 636, 771
BseNI ACTGG 3 cut(s) 160, 550, 1013
BseRI GAGGAG 1 cut(s) 464
BseXI GCAGC 1 cut(s) 1047
BsgI GTGCAG 1 cut(s) 329
BshFI GGCC 4 cut(s) 247, 522, 787, 829
BshNI GGYRCC 1 cut(s) 205
BshVI ATCGAT 1 cut(s) 620
BsiHKAI GWGCWC 1 cut(s) 121
BslFI GGGAC 2 cut(s) 449, 562
BslI CCNNNNNNNGG 1 cut(s) 22
BsmAI GTCTC 1 cut(s) 209
BsmFI GGGAC 2 cut(s) 449, 562
BsmI GAATGC 1 cut(s) 655
BsnI GGCC 4 cut(s) 247, 522, 787, 829
Bso31I GGTCTC 1 cut(s) 209
Bsp1286I GDGCHC 1 cut(s) 121
Bsp143I GATC 2 cut(s) 101, 1043
BspACI CCGC 6 cut(s) 173, 419, 715, 876, 979, 1038
BspANI GGCC 4 cut(s) 247, 522, 787, 829
BspCNI CTCAG 3 cut(s) 25, 637, 772
BspDI ATCGAT 1 cut(s) 620
BspHI TCATGA 1 cut(s) 445
BspLI GGNNCC 2 cut(s) 207, 708
BspMAI CTGCAG 2 cut(s) 866, 1037
BspPI GGATC 1 cut(s) 1051
BspQI GCTCTTC 1 cut(s) 666
BspT107I GGYRCC 1 cut(s) 205
BspTNI GGTCTC 1 cut(s) 209
BsrDI GCAATG 2 cut(s) 469, 679
BsrI ACTGG 3 cut(s) 160, 550, 1013
BssECI CCNNGG 3 cut(s) 21, 232, 517
BssMI GATC 2 cut(s) 101, 1043
BssT1I CCWWGG 2 cut(s) 21, 517
Bst2UI CCWGG 2 cut(s) 234, 753
Bst6I CTCTTC 4 cut(s) 55, 68, 425, 666
BstC8I GCNNGC 1 cut(s) 177
BstDEI CTNAG 4 cut(s) 33, 645, 780, 872
BstF5I GGATG 1 cut(s) 404
BstKTI GATC 2 cut(s) 104, 1046
BstMAI GTCTC 1 cut(s) 209
BstMBI GATC 2 cut(s) 101, 1043
BstMWI GCNNNNNNNGC 4 cut(s) 116, 172, 528, 566
BstNI CCWGG 2 cut(s) 234, 753
BstSCI CCNGG 2 cut(s) 232, 751
BstSFI CTRYAG 2 cut(s) 862, 1033
BstV1I GCAGC 1 cut(s) 1047
BstV2I GAAGAC 1 cut(s) 1031
BstXI CCANNNNNNTGG 1 cut(s) 1020
Bsu15I ATCGAT 1 cut(s) 620
BsuRI GGCC 4 cut(s) 247, 522, 787, 829
BsuTUI ATCGAT 1 cut(s) 620
BtsCI GGATG 1 cut(s) 404
BtsI GCAGTG 2 cut(s) 710, 859
BtsIMutI CAGTG 3 cut(s) 710, 777, 859
Cac8I GCNNGC 1 cut(s) 177
CciI TCATGA 1 cut(s) 445
ClaI ATCGAT 1 cut(s) 620
Csp6I GTAC 1 cut(s) 206
CviAII CATG 5 cut(s) 446, 458, 494, 539, 566
CviQI GTAC 1 cut(s) 206
DdeI CTNAG 4 cut(s) 33, 645, 780, 872
DpnI GATC 2 cut(s) 103, 1045
DpnII GATC 2 cut(s) 101, 1043
Eam1104I CTCTTC 4 cut(s) 55, 68, 425, 666
EarI CTCTTC 4 cut(s) 55, 68, 425, 666
Eco130I CCWWGG 2 cut(s) 21, 517
Eco147I AGGCCT 1 cut(s) 787
Eco31I GGTCTC 1 cut(s) 209
Eco57I CTGAAG 3 cut(s) 92, 209, 647
EcoRII CCWGG 2 cut(s) 232, 751
EcoT14I CCWWGG 2 cut(s) 21, 517
EcoT22I ATGCAT 1 cut(s) 627
ErhI CCWWGG 2 cut(s) 21, 517
FaeI CATG 5 cut(s) 449, 461, 497, 542, 569
FalI AAGNNNNNCTT 2 cut(s) 957, 989
FaqI GGGAC 2 cut(s) 449, 562
FatI CATG 5 cut(s) 445, 457, 493, 538, 565
FauI CCCGC 1 cut(s) 180
Fnu4HI GCNGC 1 cut(s) 1036
FokI GGATG 1 cut(s) 411
Fsp4HI GCNGC 1 cut(s) 1036
FspBI CTAG 2 cut(s) 341, 552
GluI GCNGC 1 cut(s) 1036
HaeIII GGCC 4 cut(s) 247, 522, 787, 829
Hin1II CATG 5 cut(s) 449, 461, 497, 542, 569
HincII GTYRAC 1 cut(s) 379
HindII GTYRAC 1 cut(s) 379
HinfI GANTC 6 cut(s) 35, 369, 562, 867, 883, 997
HphI GGTGA 3 cut(s) 929, 968, 1002
Hpy166II GTNNAC 2 cut(s) 379, 472
Hpy188I TCNGA 6 cut(s) 34, 59, 228, 666, 793, 814
Hpy188III TCNNGA 3 cut(s) 212, 257, 446
Hpy8I GTNNAC 2 cut(s) 379, 472
HpyAV CCTTC 4 cut(s) 292, 624, 798, 812
HpyCH4IV ACGT 1 cut(s) 265
HpyCH4V TGCA 9 cut(s) 110, 166, 190, 310, 402, 467, 625, 864, 1035
HpyF10VI GCNNNNNNNGC 4 cut(s) 116, 172, 528, 566
HpyF3I CTNAG 4 cut(s) 33, 645, 780, 872
HpySE526I ACGT 1 cut(s) 265
Hsp92II CATG 5 cut(s) 449, 461, 497, 542, 569
KpnI GGTACC 1 cut(s) 209
Kzo9I GATC 2 cut(s) 101, 1043
LguI GCTCTTC 1 cut(s) 666
LmnI GCTCC 1 cut(s) 989
Lsp1109I GCAGC 1 cut(s) 1047
LweI GCATC 4 cut(s) 389, 612, 634, 784
MaeI CTAG 2 cut(s) 341, 552
MaeII ACGT 1 cut(s) 265
MaeIII GTNAC 2 cut(s) 592, 1008
MalI GATC 2 cut(s) 103, 1045
MboI GATC 2 cut(s) 101, 1043
MhlI GDGCHC 1 cut(s) 121
MluCI AATT 6 cut(s) 302, 362, 614, 822, 912, 1047
MlyI GAGTC 4 cut(s) 44, 556, 892, 1006
MmeI TCCRAC 3 cut(s) 275, 820, 860
MnlI CCTC 9 cut(s) 69, 219, 426, 442, 445, 453, 662, 777, 1034
Mph1103I ATGCAT 1 cut(s) 627
MroXI GAANNNNTTC 1 cut(s) 969
MseI TTAA 2 cut(s) 834, 942
MslI CAYNNNNRTG 2 cut(s) 195, 462
MspA1I CMGCKG 2 cut(s) 175, 1038
MspR9I CCNGG 2 cut(s) 234, 753
Mva1269I GAATGC 1 cut(s) 655
MvaI CCWGG 2 cut(s) 234, 753
MwoI GCNNNNNNNGC 4 cut(s) 116, 172, 528, 566
NdeII GATC 2 cut(s) 101, 1043
NlaIII CATG 5 cut(s) 449, 461, 497, 542, 569
NlaIV GGNNCC 2 cut(s) 207, 708
NmuCI GTSAC 1 cut(s) 1008
NsiI ATGCAT 1 cut(s) 627
PagI TCATGA 1 cut(s) 445
PceI AGGCCT 1 cut(s) 787
PciSI GCTCTTC 1 cut(s) 666
PctI GAATGC 1 cut(s) 655
PdmI GAANNNNTTC 1 cut(s) 969
PfeI GAWTC 2 cut(s) 369, 867
PkrI GCNGC 1 cut(s) 1037
PleI GAGTC 4 cut(s) 43, 556, 891, 1005
PpsI GAGTC 4 cut(s) 43, 556, 891, 1005
Psp6I CCWGG 2 cut(s) 232, 751
PspGI CCWGG 2 cut(s) 232, 751
PspN4I GGNNCC 2 cut(s) 207, 708
PstI CTGCAG 2 cut(s) 866, 1037
RsaI GTAC 1 cut(s) 207
RsaNI GTAC 1 cut(s) 206
RseI CAYNNNNRTG 2 cut(s) 195, 462
SapI GCTCTTC 1 cut(s) 666
SaqAI TTAA 2 cut(s) 834, 942
SatI GCNGC 1 cut(s) 1036
Sau3AI GATC 2 cut(s) 101, 1043
SchI GAGTC 4 cut(s) 44, 556, 892, 1006
ScrFI CCNGG 2 cut(s) 234, 753
SduI GDGCHC 1 cut(s) 121
SfaNI GCATC 4 cut(s) 389, 612, 634, 784
SfcI CTRYAG 2 cut(s) 862, 1033
SmiMI CAYNNNNRTG 2 cut(s) 195, 462
SmlI CTYRAG 1 cut(s) 210
SmoI CTYRAG 1 cut(s) 210
SpeI ACTAGT 1 cut(s) 551
Sse9I AATT 6 cut(s) 302, 362, 614, 822, 912, 1047
SseBI AGGCCT 1 cut(s) 787
SsiI CCGC 6 cut(s) 173, 419, 715, 876, 979, 1038
SspI AATATT 1 cut(s) 574
SspMI CTAG 2 cut(s) 341, 552
StuI AGGCCT 1 cut(s) 787
StyD4I CCNGG 2 cut(s) 232, 751
StyI CCWWGG 2 cut(s) 21, 517
TaiI ACGT 1 cut(s) 268
TaqI TCGA 4 cut(s) 349, 620, 628, 688
TasI AATT 6 cut(s) 302, 362, 614, 822, 912, 1047
TfiI GAWTC 2 cut(s) 369, 867
Tru1I TTAA 2 cut(s) 834, 942
Tru9I TTAA 2 cut(s) 834, 942
TscAI CASTG 3 cut(s) 717, 784, 866
TseFI GTSAC 1 cut(s) 1008
TseI GCWGC 1 cut(s) 1035
Tsp45I GTSAC 1 cut(s) 1008
TspDTI ATGAA 3 cut(s) 372, 603, 627
TspGWI ACGGA 1 cut(s) 42
TspRI CASTG 3 cut(s) 717, 784, 866
XapI RAATTY 2 cut(s) 302, 822
XcmI CCANNNNNNNNNTGG 1 cut(s) 240
XmnI GAANNNNTTC 1 cut(s) 969
XspI CTAG 2 cut(s) 341, 552
Zsp2I ATGCAT 1 cut(s) 627
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.