Rw6G017210

B3 DNA binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0000593
Physical Location & Seq
Reverse (-)
34170115 .. 34171745
1631 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G017210.1

Sequence Viewer

Length: 1047 bp
ATGGCAAGAACAACAAGACCAGTAAGAAGAAGAAAACCCTCCTTTTTCAAGGTTCTTATAGGTGACTTCTCTAACCACCTGCAAATCCCGCCGGCTTTTTATAAGCACTTTGATGGGAAGGGACCTCAGCAGACCCACCTTCGGACCCTGGCTAAAACTTGGCTTGTTAATGTGGAAAAGATTGATGGCAAGTTCTTCTTCCGAAAGGGTTGGAAGAAATTTGTGCATGGAAATGGTCTGAAGCTTGGTGAATTTCTTGTTTTTCGATATTTTGGAAACTCGAAATTCTTTGTTGACATATATGGAAGAAATACCTGCAAAAAGGATTTTGTAAAGGCCGTTGAGAAAAGTGAGAAAGAGGGACATGGTAATGAAGATTACCAGGGCTTAAAAGGTGTAGCTGAAGGAACTAGGACTGGTCAAGCCACGAACAGTATTCACATCAAAGATGAAGTAATCGATACTGAATTGGAAAGATCGTTGCATCCATTGACCAGAAATACTGGAAAAGATGTCGCTCTTGAGAGAGCAAAAGCTTTCAAAATTGAAGATGAAGAAAAACAAGGAAATGATGATGAGGACGAGGATGATGATGATTCTATAAGAATCCCAAATGACCTTCCACCATGCACAAAAACAATGGAGAAATCTCCATTAGGAGCACAACTTGTCAAGAGAAGGCAATCAAGTTCTAGTACTAACATTGGTGGAAAAGATGTAGCACTTCAGAAAGCCAATGCTTTCAAATCTGCAGACCCTTCTTTCGTGGTTGCAATGCATATCTCGTATGTCCACGCAGGTTATATGAATGTGCCATCTGCTTTTGCCAAGGGATATCTTACTAAGCGGCCTAGTGAAGTCACCCTCCAGGTTAATGGGGAAACTTGGTCTGTCAATTTACAATATGAAAGAAACATGAAAAGACTCACCACAGGTTGGGCGGATTTTGTGCGAGCCAATAAGTTGGAAGTTGGTAACGCGTGTGTTTTTGTGTTGAGTAACGACGACAAGTTTATCTTTGATGTTATCGTTTACCGCGCCAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

348

Amino Acids

39.48

Weight (kDa)

9.34

Isoelectric Point (pI)

38.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 15 - 103 7.1e-15 B3 DNA binding domain
B3 PF02362 256 - 344 2.6e-15 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 102
AarI CACCTGC 1 cut(s) 87
Acc36I ACCTGC 3 cut(s) 87, 323, 788
AccB7I CCANNNNNTGG 1 cut(s) 936
AccII CGCG 2 cut(s) 980, 1038
AciI CCGC 4 cut(s) 89, 847, 941, 1036
AcsI RAATTY 3 cut(s) 218, 251, 284
AcuI CTGAAG 3 cut(s) 260, 423, 710
AfaI GTAC 1 cut(s) 697
AfiI CCNNNNNNNGG 2 cut(s) 141, 936
AflIII ACRYGT 1 cut(s) 978
AgsI TTSAA 4 cut(s) 49, 541, 548, 745
AjnI CCWGG 3 cut(s) 147, 381, 867
AluBI AGCT 3 cut(s) 244, 401, 536
AluI AGCT 3 cut(s) 244, 401, 536
Alw21I GWGCWC 1 cut(s) 664
AoxI GGCC 2 cut(s) 336, 848
ApoI RAATTY 3 cut(s) 218, 251, 284
ArsI GACNNNNNNTTYG 2 cut(s) 746, 778
AspLEI GCGC 1 cut(s) 1040
AspS9I GGNCC 2 cut(s) 122, 144
AsuHPI GGTGA 4 cut(s) 74, 260, 853, 919
AvaII GGWCC 2 cut(s) 122, 144
Bbv12I GWGCWC 1 cut(s) 664
BbvCI CCTCAGC 1 cut(s) 126
BccI CCATC 3 cut(s) 107, 179, 823
BceAI ACGGC 1 cut(s) 323
BciT130I CCWGG 3 cut(s) 149, 383, 869
BfaI CTAG 3 cut(s) 411, 693, 852
BfmI CTRYAG 1 cut(s) 750
BfuAI ACCTGC 3 cut(s) 87, 323, 788
BisI GCNGC 1 cut(s) 848
BlsI GCNGC 1 cut(s) 849
BmcAI AGTACT 1 cut(s) 697
Bme1390I CCNGG 3 cut(s) 149, 383, 869
Bme18I GGWCC 2 cut(s) 122, 144
BmgT120I GGNCC 2 cut(s) 122, 144
BmiI GGNNCC 2 cut(s) 123, 146
BmrFI CCNGG 3 cut(s) 149, 383, 869
BmsI GCATC 1 cut(s) 493
BpmI CTGGAG 1 cut(s) 851
Bpu10I CCTNAGC 1 cut(s) 126
BpuEI CTTGAG 1 cut(s) 542
Bsa29I ATCGAT 1 cut(s) 459
BsaJI CCNNGG 3 cut(s) 147, 382, 828
Bsc4I CCNNNNNNNGG 2 cut(s) 141, 936
Bse118I RCCGGY 1 cut(s) 91
Bse1I ACTGG 3 cut(s) 20, 421, 508
Bse3DI GCAATG 1 cut(s) 780
BseBI CCWGG 3 cut(s) 149, 383, 869
BseCI ATCGAT 1 cut(s) 459
BseDI CCNNGG 3 cut(s) 147, 382, 828
BseGI GGATG 2 cut(s) 484, 592
BseLI CCNNNNNNNGG 2 cut(s) 141, 936
BseMI GCAATG 1 cut(s) 780
BseMII CTCAG 1 cut(s) 140
BseNI ACTGG 3 cut(s) 20, 421, 508
Bsh1236I CGCG 2 cut(s) 980, 1038
BshFI GGCC 2 cut(s) 338, 850
BshVI ATCGAT 1 cut(s) 459
BsiHKAI GWGCWC 1 cut(s) 664
BsiSI CCGG 1 cut(s) 92
BslFI GGGAC 2 cut(s) 135, 375
BslI CCNNNNNNNGG 2 cut(s) 141, 936
BsmFI GGGAC 2 cut(s) 135, 375
BsnI GGCC 2 cut(s) 338, 850
Bsp1286I GDGCHC 1 cut(s) 664
Bsp143I GATC 1 cut(s) 476
BspACI CCGC 4 cut(s) 89, 847, 941, 1036
BspANI GGCC 2 cut(s) 338, 850
BspCNI CTCAG 1 cut(s) 139
BspDI ATCGAT 1 cut(s) 459
BspFNI CGCG 2 cut(s) 980, 1038
BspLI GGNNCC 2 cut(s) 123, 146
BspMAI CTGCAG 1 cut(s) 754
BspMI ACCTGC 3 cut(s) 87, 323, 788
BsrDI GCAATG 1 cut(s) 780
BsrFI RCCGGY 1 cut(s) 91
BsrI ACTGG 3 cut(s) 20, 421, 508
BssAI RCCGGY 1 cut(s) 91
BssECI CCNNGG 3 cut(s) 147, 382, 828
BssMI GATC 1 cut(s) 476
BssT1I CCWWGG 1 cut(s) 828
Bst2UI CCWGG 3 cut(s) 149, 383, 869
Bst4CI ACNGT 1 cut(s) 434
BstC8I GCNNGC 2 cut(s) 93, 954
BstDEI CTNAG 2 cut(s) 126, 843
BstF5I GGATG 2 cut(s) 484, 592
BstFNI CGCG 2 cut(s) 980, 1038
BstHHI GCGC 1 cut(s) 1040
BstKTI GATC 1 cut(s) 479
BstMBI GATC 1 cut(s) 476
BstMWI GCNNNNNNNGC 1 cut(s) 88
BstNI CCWGG 3 cut(s) 149, 383, 869
BstSCI CCNGG 3 cut(s) 147, 381, 867
BstSFI CTRYAG 1 cut(s) 750
BstUI CGCG 2 cut(s) 980, 1038
BstXI CCANNNNNNTGG 2 cut(s) 875, 964
Bsu15I ATCGAT 1 cut(s) 459
BsuRI GGCC 2 cut(s) 338, 850
BsuTUI ATCGAT 1 cut(s) 459
BtsCI GGATG 2 cut(s) 484, 592
BveI ACCTGC 3 cut(s) 87, 323, 788
Cac8I GCNNGC 2 cut(s) 93, 954
CfoI GCGC 1 cut(s) 1040
Cfr10I RCCGGY 1 cut(s) 91
Cfr13I GGNCC 2 cut(s) 122, 144
ClaI ATCGAT 1 cut(s) 459
Csp6I GTAC 1 cut(s) 696
CviAII CATG 4 cut(s) 227, 365, 627, 916
CviQI GTAC 1 cut(s) 696
DdeI CTNAG 2 cut(s) 126, 843
DpnI GATC 1 cut(s) 478
DpnII GATC 1 cut(s) 476
EciI GGCGGA 1 cut(s) 956
Eco130I CCWWGG 1 cut(s) 828
Eco32I GATATC 1 cut(s) 836
Eco47I GGWCC 2 cut(s) 122, 144
Eco57I CTGAAG 3 cut(s) 260, 423, 710
EcoO109I RGGNCCY 1 cut(s) 122
EcoRII CCWGG 3 cut(s) 147, 381, 867
EcoRV GATATC 1 cut(s) 836
EcoT14I CCWWGG 1 cut(s) 828
EcoT22I ATGCAT 1 cut(s) 780
ErhI CCWWGG 1 cut(s) 828
FaeI CATG 4 cut(s) 230, 368, 630, 919
FalI AAGNNNNNCTT 4 cut(s) 182, 214, 1001, 1033
FaqI GGGAC 2 cut(s) 135, 375
FatI CATG 4 cut(s) 226, 364, 626, 915
FauI CCCGC 1 cut(s) 96
Fnu4HI GCNGC 1 cut(s) 848
FokI GGATG 2 cut(s) 471, 599
Fsp4HI GCNGC 1 cut(s) 848
FspBI CTAG 3 cut(s) 411, 693, 852
GlaI GCGC 1 cut(s) 1039
GluI GCNGC 1 cut(s) 848
GsuI CTGGAG 1 cut(s) 851
HaeIII GGCC 2 cut(s) 338, 850
HapII CCGG 1 cut(s) 92
HhaI GCGC 1 cut(s) 1040
Hin1II CATG 4 cut(s) 230, 368, 630, 919
Hin6I GCGC 1 cut(s) 1038
HinP1I GCGC 1 cut(s) 1038
HincII GTYRAC 1 cut(s) 295
HindII GTYRAC 1 cut(s) 295
HindIII AAGCTT 2 cut(s) 242, 534
HinfI GANTC 3 cut(s) 596, 606, 924
HpaII CCGG 1 cut(s) 92
HphI GGTGA 4 cut(s) 74, 260, 853, 919
Hpy166II GTNNAC 3 cut(s) 295, 793, 1033
Hpy188I TCNGA 4 cut(s) 144, 203, 240, 729
Hpy188III TCNNGA 2 cut(s) 521, 673
Hpy8I GTNNAC 3 cut(s) 295, 793, 1033
Hpy99I CGWCG 1 cut(s) 1007
HpyAV CCTTC 6 cut(s) 112, 149, 398, 629, 672, 768
HpyCH4III ACNGT 1 cut(s) 434
HpyCH4V TGCA 8 cut(s) 82, 226, 318, 484, 630, 752, 773, 778
HpyF10VI GCNNNNNNNGC 1 cut(s) 88
HpyF3I CTNAG 2 cut(s) 126, 843
Hsp92II CATG 4 cut(s) 230, 368, 630, 919
HspAI GCGC 1 cut(s) 1038
KroI GCCGGC 1 cut(s) 91
KroNI GCCGGC 1 cut(s) 93
Kzo9I GATC 1 cut(s) 476
LmnI GCTCC 1 cut(s) 659
LweI GCATC 1 cut(s) 493
MaeI CTAG 3 cut(s) 411, 693, 852
MaeIII GTNAC 4 cut(s) 62, 859, 974, 998
MalI GATC 1 cut(s) 478
MboI GATC 1 cut(s) 476
MboII GAAGA 9 cut(s) 39, 42, 187, 190, 226, 318, 386, 560, 566
MhlI GDGCHC 1 cut(s) 664
MluCI AATT 6 cut(s) 218, 251, 284, 467, 543, 895
MluI ACGCGT 1 cut(s) 978
MlyI GAGTC 1 cut(s) 918
MmeI TCCRAC 2 cut(s) 191, 945
MnlI CCTC 6 cut(s) 49, 135, 352, 571, 577, 875
Mph1103I ATGCAT 1 cut(s) 780
MroNI GCCGGC 1 cut(s) 91
MseI TTAA 3 cut(s) 168, 389, 873
MslI CAYNNNNRTG 3 cut(s) 111, 231, 369
MspI CCGG 1 cut(s) 92
MspR9I CCNGG 3 cut(s) 149, 383, 869
MvaI CCWGG 3 cut(s) 149, 383, 869
MvnI CGCG 2 cut(s) 980, 1038
MwoI GCNNNNNNNGC 1 cut(s) 88
NaeI GCCGGC 1 cut(s) 93
NdeII GATC 1 cut(s) 476
NgoMIV GCCGGC 1 cut(s) 91
NlaIII CATG 4 cut(s) 230, 368, 630, 919
NlaIV GGNNCC 2 cut(s) 123, 146
NmuCI GTSAC 2 cut(s) 62, 859
NsiI ATGCAT 1 cut(s) 780
PaqCI CACCTGC 1 cut(s) 87
PdiI GCCGGC 1 cut(s) 93
PfeI GAWTC 2 cut(s) 596, 606
PflMI CCANNNNNTGG 1 cut(s) 936
PkrI GCNGC 1 cut(s) 849
PleI GAGTC 1 cut(s) 918
PpsI GAGTC 1 cut(s) 918
PpuMI RGGWCCY 1 cut(s) 122
PsiI TTATAA 1 cut(s) 102
Psp5II RGGWCCY 1 cut(s) 122
Psp6I CCWGG 3 cut(s) 147, 381, 867
PspGI CCWGG 3 cut(s) 147, 381, 867
PspN4I GGNNCC 2 cut(s) 123, 146
PspPI GGNCC 2 cut(s) 122, 144
PspPPI RGGWCCY 1 cut(s) 122
PstI CTGCAG 1 cut(s) 754
RsaI GTAC 1 cut(s) 697
RsaNI GTAC 1 cut(s) 696
RseI CAYNNNNRTG 3 cut(s) 111, 231, 369
SaqAI TTAA 3 cut(s) 168, 389, 873
SatI GCNGC 1 cut(s) 848
Sau3AI GATC 1 cut(s) 476
Sau96I GGNCC 2 cut(s) 122, 144
ScaI AGTACT 1 cut(s) 697
SchI GAGTC 1 cut(s) 918
ScrFI CCNGG 3 cut(s) 149, 383, 869
SduI GDGCHC 1 cut(s) 664
SfaNI GCATC 1 cut(s) 493
SfcI CTRYAG 1 cut(s) 750
SinI GGWCC 2 cut(s) 122, 144
SmiMI CAYNNNNRTG 3 cut(s) 111, 231, 369
SmlI CTYRAG 1 cut(s) 521
SmoI CTYRAG 1 cut(s) 521
Sse9I AATT 6 cut(s) 218, 251, 284, 467, 543, 895
SsiI CCGC 4 cut(s) 89, 847, 941, 1036
SspMI CTAG 3 cut(s) 411, 693, 852
StyD4I CCNGG 3 cut(s) 147, 381, 867
StyI CCWWGG 1 cut(s) 828
TaaI ACNGT 1 cut(s) 434
TaqI TCGA 3 cut(s) 265, 281, 459
TasI AATT 6 cut(s) 218, 251, 284, 467, 543, 895
TatI WGTACW 1 cut(s) 695
TauI GCSGC 1 cut(s) 850
TfiI GAWTC 2 cut(s) 596, 606
Tru1I TTAA 3 cut(s) 168, 389, 873
Tru9I TTAA 3 cut(s) 168, 389, 873
TseFI GTSAC 2 cut(s) 62, 859
Tsp45I GTSAC 2 cut(s) 62, 859
TspDTI ATGAA 6 cut(s) 387, 465, 567, 821, 921, 932
Van91I CCANNNNNTGG 1 cut(s) 936
VpaK11BI GGWCC 2 cut(s) 122, 144
XapI RAATTY 3 cut(s) 218, 251, 284
XspI CTAG 3 cut(s) 411, 693, 852
ZrmI AGTACT 1 cut(s) 697
Zsp2I ATGCAT 1 cut(s) 780
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.