Rh6BG201300

B3 DNA binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0000593
Physical Location & Seq
Reverse (-)
37919546 .. 37922431
2886 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG201300.1

Sequence Viewer

Length: 339 bp
ATGGCAAGAACAACAAGACCAGTAAGAAGAAGAAAACCCTCCTTCTTCAAGGTTCTTATAGGTGACTTCTCTAACCACCTGCAAATCCCGCCGGCTTTTTATAAGCACTTTGATGGGAAGGGACCTCAGCAGACCCACCTTCGGACCCTGGCTAAAACTTGGCTTGTTAATGTGGAAAAGATTGATGGCAAGTTCTTCTTCCGAAAGGGTTGGAAGAAATTTGTGCATTTAAATGGTCTGAAGCTTGGTTTGCACCCAAGGGTTATGGATGGTGGCTACGCCATCTCTCAATGGGCTGAGATCGGTGGTGGCTACCTGTCTCTTTCAGAACTAGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

112

Amino Acids

12.85

Weight (kDa)

10.52

Isoelectric Point (pI)

38.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 15 - 83 4.6e-09 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 102
AarI CACCTGC 1 cut(s) 87
Acc36I ACCTGC 1 cut(s) 87
AciI CCGC 1 cut(s) 89
AcsI RAATTY 1 cut(s) 218
AcuI CTGAAG 1 cut(s) 260
AfiI CCNNNNNNNGG 1 cut(s) 141
AgsI TTSAA 1 cut(s) 49
AjnI CCWGG 1 cut(s) 147
AluBI AGCT 2 cut(s) 244, 335
AluI AGCT 2 cut(s) 244, 335
Alw26I GTCTC 1 cut(s) 324
ApoI RAATTY 1 cut(s) 218
AspS9I GGNCC 2 cut(s) 122, 144
AsuHPI GGTGA 1 cut(s) 74
AvaII GGWCC 2 cut(s) 122, 144
BbvCI CCTCAGC 1 cut(s) 126
BccI CCATC 4 cut(s) 107, 179, 263, 290
BciT130I CCWGG 1 cut(s) 149
BcoDI GTCTC 1 cut(s) 324
BfaI CTAG 1 cut(s) 332
BfuAI ACCTGC 1 cut(s) 87
Bme1390I CCNGG 1 cut(s) 149
Bme18I GGWCC 2 cut(s) 122, 144
BmgT120I GGNCC 2 cut(s) 122, 144
BmiI GGNNCC 2 cut(s) 123, 146
BmrFI CCNGG 1 cut(s) 149
Bpu10I CCTNAGC 1 cut(s) 126
BsaJI CCNNGG 2 cut(s) 147, 257
Bsc4I CCNNNNNNNGG 1 cut(s) 141
Bse118I RCCGGY 1 cut(s) 91
Bse1I ACTGG 1 cut(s) 20
BseBI CCWGG 1 cut(s) 149
BseDI CCNNGG 2 cut(s) 147, 257
BseGI GGATG 1 cut(s) 274
BseLI CCNNNNNNNGG 1 cut(s) 141
BseMII CTCAG 2 cut(s) 140, 288
BseNI ACTGG 1 cut(s) 20
BsiSI CCGG 1 cut(s) 92
BslFI GGGAC 1 cut(s) 135
BslI CCNNNNNNNGG 1 cut(s) 141
BsmAI GTCTC 1 cut(s) 324
BsmFI GGGAC 1 cut(s) 135
Bsp143I GATC 1 cut(s) 300
BspACI CCGC 1 cut(s) 89
BspCNI CTCAG 2 cut(s) 139, 289
BspLI GGNNCC 2 cut(s) 123, 146
BspMI ACCTGC 1 cut(s) 87
BsrFI RCCGGY 1 cut(s) 91
BsrI ACTGG 1 cut(s) 20
BssAI RCCGGY 1 cut(s) 91
BssECI CCNNGG 2 cut(s) 147, 257
BssMI GATC 1 cut(s) 300
BssT1I CCWWGG 1 cut(s) 257
Bst2UI CCWGG 1 cut(s) 149
BstC8I GCNNGC 1 cut(s) 93
BstDEI CTNAG 2 cut(s) 126, 297
BstF5I GGATG 1 cut(s) 274
BstKTI GATC 1 cut(s) 303
BstMAI GTCTC 1 cut(s) 324
BstMBI GATC 1 cut(s) 300
BstMWI GCNNNNNNNGC 2 cut(s) 88, 250
BstNI CCWGG 1 cut(s) 149
BstSCI CCNGG 1 cut(s) 147
BtsCI GGATG 1 cut(s) 274
BveI ACCTGC 1 cut(s) 87
Cac8I GCNNGC 1 cut(s) 93
Cfr10I RCCGGY 1 cut(s) 91
Cfr13I GGNCC 2 cut(s) 122, 144
CviJI RGCY 8 cut(s) 95, 152, 163, 244, 276, 296, 312, 335
CviKI_1 RGCY 8 cut(s) 95, 152, 163, 244, 276, 296, 312, 335
DdeI CTNAG 2 cut(s) 126, 297
DpnI GATC 1 cut(s) 302
DpnII GATC 1 cut(s) 300
DraI TTTAAA 1 cut(s) 231
Eco130I CCWWGG 1 cut(s) 257
Eco47I GGWCC 2 cut(s) 122, 144
Eco57I CTGAAG 1 cut(s) 260
EcoO109I RGGNCCY 1 cut(s) 122
EcoRII CCWGG 1 cut(s) 147
EcoT14I CCWWGG 1 cut(s) 257
ErhI CCWWGG 1 cut(s) 257
FaiI YATR 3 cut(s) 59, 102, 266
FalI AAGNNNNNCTT 2 cut(s) 182, 214
FaqI GGGAC 1 cut(s) 135
FauI CCCGC 1 cut(s) 96
FokI GGATG 1 cut(s) 281
FspBI CTAG 1 cut(s) 332
HapII CCGG 1 cut(s) 92
HindIII AAGCTT 1 cut(s) 242
HpaII CCGG 1 cut(s) 92
HphI GGTGA 1 cut(s) 74
Hpy188I TCNGA 4 cut(s) 144, 203, 240, 328
HpyAV CCTTC 3 cut(s) 52, 112, 149
HpyCH4V TGCA 3 cut(s) 82, 226, 253
HpyF10VI GCNNNNNNNGC 2 cut(s) 88, 250
HpyF3I CTNAG 2 cut(s) 126, 297
KroI GCCGGC 1 cut(s) 91
KroNI GCCGGC 1 cut(s) 93
Kzo9I GATC 1 cut(s) 300
LpnPI CCDG 6 cut(s) 33, 92, 105, 134, 161, 329
MaeI CTAG 1 cut(s) 332
MaeIII GTNAC 1 cut(s) 62
MalI GATC 1 cut(s) 302
MboI GATC 1 cut(s) 300
MboII GAAGA 6 cut(s) 37, 39, 42, 187, 190, 226
MluCI AATT 1 cut(s) 218
MmeI TCCRAC 1 cut(s) 191
MnlI CCTC 2 cut(s) 49, 135
MroNI GCCGGC 1 cut(s) 91
MseI TTAA 2 cut(s) 168, 230
MslI CAYNNNNRTG 2 cut(s) 111, 231
MspI CCGG 1 cut(s) 92
MspR9I CCNGG 1 cut(s) 149
MvaI CCWGG 1 cut(s) 149
MwoI GCNNNNNNNGC 2 cut(s) 88, 250
NaeI GCCGGC 1 cut(s) 93
NdeII GATC 1 cut(s) 300
NgoMIV GCCGGC 1 cut(s) 91
NlaIV GGNNCC 2 cut(s) 123, 146
NmuCI GTSAC 1 cut(s) 62
PaqCI CACCTGC 1 cut(s) 87
PdiI GCCGGC 1 cut(s) 93
PpuMI RGGWCCY 1 cut(s) 122
PsiI TTATAA 1 cut(s) 102
Psp5II RGGWCCY 1 cut(s) 122
Psp6I CCWGG 1 cut(s) 147
PspGI CCWGG 1 cut(s) 147
PspN4I GGNNCC 2 cut(s) 123, 146
PspPI GGNCC 2 cut(s) 122, 144
PspPPI RGGWCCY 1 cut(s) 122
RseI CAYNNNNRTG 2 cut(s) 111, 231
SaqAI TTAA 2 cut(s) 168, 230
Sau3AI GATC 1 cut(s) 300
Sau96I GGNCC 2 cut(s) 122, 144
ScrFI CCNGG 1 cut(s) 149
SetI ASST 8 cut(s) 54, 64, 81, 127, 141, 246, 318, 337
SinI GGWCC 2 cut(s) 122, 144
SmiI ATTTAAAT 1 cut(s) 231
SmiMI CAYNNNNRTG 2 cut(s) 111, 231
Sse9I AATT 1 cut(s) 218
SsiI CCGC 1 cut(s) 89
SspMI CTAG 1 cut(s) 332
StyD4I CCNGG 1 cut(s) 147
StyI CCWWGG 1 cut(s) 257
SwaI ATTTAAAT 1 cut(s) 231
TasI AATT 1 cut(s) 218
Tru1I TTAA 2 cut(s) 168, 230
Tru9I TTAA 2 cut(s) 168, 230
TseFI GTSAC 1 cut(s) 62
Tsp45I GTSAC 1 cut(s) 62
VpaK11BI GGWCC 2 cut(s) 122, 144
XapI RAATTY 1 cut(s) 218
XspI CTAG 1 cut(s) 332
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.