Rroxscaffold_7G00194250

B3 DNA binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0000593
Physical Location & Seq
Forward (+)
36009837 .. 36011409
1573 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00194250.1

Sequence Viewer

Length: 1035 bp
ATGGCAAGAGCAACAAGAAGAAAACCCTCCTTCTTCAAGGTTCTTATAGGTGACTTCTCTAACCACCTGCAAATCCCGCCTGCTTTTTGTAAGCACTTTGATGGGAAGGGACCTCAGCAGACCCACCTTCGGACCCTGGCTAAAACTTGGCTTGTTAATGTGGAAAAGATTGATGGCAAGTTCTTCTTCCGAAAGGGTTGGAAGAAATTTGTGCATGGCAATGGTCTGAAGCTTGGTGAATTTCTTGTTTTTCGATATTTTGGAAAATCGAAATTCTTTGTTGACATATATGGAAGAAATACCTGCAAAAAGGATTTTGTAAAGGCCGTTGAGAAAAGTGAGAAAGAGGGACATGGTAATGAAGATATCCAGGGCTTAAAAGGTGTAGCTGAAGGAGCTAGGACTGGTCAAGCCACGAACAGTATTCACATCAAAGATGAAGTAATTGATACTGAATCGGAAAGATCGTTGCATCCATTGACAAGAAATACTGGAAAAGATGTTGCTCTTGAGAGAGCAAACGCTTTCAAAATTGAAGATGAAGAAGAACCAGGAAATGATGATGAGGACGAGGATGATGATGATTCTATTAGAATCCCAAATGACCTTCCACCATGCACAAAAACAATGGAGAAATCTCCATTAGGACCACAACTTGTCAAGAGAAGGCAATCAAGTTCTAGTACTAACATTGATGGAAAAGATGTAGCACTTCAGAAAGCCAACGCTTTCAAATCTGCAGACCCTTCTTTCGTGGTTCCAATGCATATTTCGTATGTCCACGCAGGTTATATGAATGTGCCATCTGCTTTTGCCAAGGGATATCTTACTAAGCGGCCTAGTGAAGTCACTCTCCAGGTTAATGGGGAAACTTGGTCTGTCAATTTACAATATGAAAGAAACATGAAAAGACTCACCACAGGTTGGGCGGATTTTGTGCGAGCCAATAAGTTGGAAGTTGGTAACGCGTGTGTTTTTGTGTTGAGCAACCACGACAGGTTTATCTTCGATGTTATCGTTTACCGCGCCAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

344

Amino Acids

38.91

Weight (kDa)

9.04

Isoelectric Point (pI)

38.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 11 - 99 4.6e-15 B3 DNA binding domain
B3 PF02362 255 - 340 6.2e-15 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 75
Acc36I ACCTGC 3 cut(s) 75, 311, 776
AccB7I CCANNNNNTGG 1 cut(s) 924
AccII CGCG 2 cut(s) 968, 1026
AciI CCGC 4 cut(s) 77, 835, 929, 1024
AcsI RAATTY 3 cut(s) 206, 239, 272
AcuI CTGAAG 3 cut(s) 248, 411, 698
AfaI GTAC 1 cut(s) 685
AfiI CCNNNNNNNGG 2 cut(s) 129, 924
AflIII ACRYGT 1 cut(s) 966
AgsI TTSAA 4 cut(s) 37, 529, 536, 733
AjnI CCWGG 4 cut(s) 135, 369, 550, 855
AjuI GAANNNNNNNTTGG 2 cut(s) 754, 786
AluBI AGCT 3 cut(s) 232, 389, 398
AluI AGCT 3 cut(s) 232, 389, 398
AoxI GGCC 2 cut(s) 324, 836
ApoI RAATTY 3 cut(s) 206, 239, 272
ArsI GACNNNNNNTTYG 2 cut(s) 734, 766
AspLEI GCGC 1 cut(s) 1028
AspS9I GGNCC 3 cut(s) 110, 132, 647
AsuHPI GGTGA 3 cut(s) 62, 248, 907
AvaII GGWCC 3 cut(s) 110, 132, 647
BbvCI CCTCAGC 1 cut(s) 114
BccI CCATC 4 cut(s) 95, 167, 689, 811
BceAI ACGGC 1 cut(s) 311
BciT130I CCWGG 4 cut(s) 137, 371, 552, 857
BfaI CTAG 3 cut(s) 399, 681, 840
BfmI CTRYAG 1 cut(s) 738
BfuAI ACCTGC 3 cut(s) 75, 311, 776
BisI GCNGC 1 cut(s) 836
BlsI GCNGC 1 cut(s) 837
BmcAI AGTACT 1 cut(s) 685
Bme1390I CCNGG 4 cut(s) 137, 371, 552, 857
Bme18I GGWCC 3 cut(s) 110, 132, 647
BmgT120I GGNCC 3 cut(s) 110, 132, 647
BmiI GGNNCC 3 cut(s) 111, 134, 759
BmrFI CCNGG 4 cut(s) 137, 371, 552, 857
BmsI GCATC 1 cut(s) 481
BpmI CTGGAG 1 cut(s) 839
Bpu10I CCTNAGC 1 cut(s) 114
BpuEI CTTGAG 1 cut(s) 530
BsaJI CCNNGG 3 cut(s) 135, 370, 816
Bsc4I CCNNNNNNNGG 2 cut(s) 129, 924
Bse1I ACTGG 2 cut(s) 409, 496
Bse3DI GCAATG 1 cut(s) 226
BseBI CCWGG 4 cut(s) 137, 371, 552, 857
BseDI CCNNGG 3 cut(s) 135, 370, 816
BseGI GGATG 2 cut(s) 472, 580
BseLI CCNNNNNNNGG 2 cut(s) 129, 924
BseMI GCAATG 1 cut(s) 226
BseMII CTCAG 1 cut(s) 128
BseNI ACTGG 2 cut(s) 409, 496
Bsh1236I CGCG 2 cut(s) 968, 1026
BshFI GGCC 2 cut(s) 326, 838
BslFI GGGAC 2 cut(s) 123, 363
BslI CCNNNNNNNGG 2 cut(s) 129, 924
BsmFI GGGAC 2 cut(s) 123, 363
BsnI GGCC 2 cut(s) 326, 838
Bsp143I GATC 1 cut(s) 464
BspACI CCGC 4 cut(s) 77, 835, 929, 1024
BspANI GGCC 2 cut(s) 326, 838
BspCNI CTCAG 1 cut(s) 127
BspFNI CGCG 2 cut(s) 968, 1026
BspLI GGNNCC 3 cut(s) 111, 134, 759
BspMAI CTGCAG 1 cut(s) 742
BspMI ACCTGC 3 cut(s) 75, 311, 776
BsrDI GCAATG 1 cut(s) 226
BsrI ACTGG 2 cut(s) 409, 496
BssECI CCNNGG 3 cut(s) 135, 370, 816
BssMI GATC 1 cut(s) 464
BssT1I CCWWGG 1 cut(s) 816
Bst2UI CCWGG 4 cut(s) 137, 371, 552, 857
Bst4CI ACNGT 1 cut(s) 422
BstC8I GCNNGC 2 cut(s) 81, 942
BstDEI CTNAG 2 cut(s) 114, 831
BstF5I GGATG 2 cut(s) 472, 580
BstFNI CGCG 2 cut(s) 968, 1026
BstHHI GCGC 1 cut(s) 1028
BstKTI GATC 1 cut(s) 467
BstMBI GATC 1 cut(s) 464
BstMWI GCNNNNNNNGC 2 cut(s) 76, 395
BstNI CCWGG 4 cut(s) 137, 371, 552, 857
BstSCI CCNGG 4 cut(s) 135, 369, 550, 855
BstSFI CTRYAG 1 cut(s) 738
BstUI CGCG 2 cut(s) 968, 1026
BstXI CCANNNNNNTGG 2 cut(s) 863, 952
BsuRI GGCC 2 cut(s) 326, 838
BtsCI GGATG 2 cut(s) 472, 580
BveI ACCTGC 3 cut(s) 75, 311, 776
Cac8I GCNNGC 2 cut(s) 81, 942
CfoI GCGC 1 cut(s) 1028
Cfr13I GGNCC 3 cut(s) 110, 132, 647
Csp6I GTAC 1 cut(s) 684
CviAII CATG 4 cut(s) 215, 353, 615, 904
CviQI GTAC 1 cut(s) 684
DdeI CTNAG 2 cut(s) 114, 831
DpnI GATC 1 cut(s) 466
DpnII GATC 1 cut(s) 464
EciI GGCGGA 1 cut(s) 944
Eco130I CCWWGG 1 cut(s) 816
Eco32I GATATC 2 cut(s) 367, 824
Eco47I GGWCC 3 cut(s) 110, 132, 647
Eco57I CTGAAG 3 cut(s) 248, 411, 698
EcoO109I RGGNCCY 1 cut(s) 110
EcoRII CCWGG 4 cut(s) 135, 369, 550, 855
EcoRV GATATC 2 cut(s) 367, 824
EcoT14I CCWWGG 1 cut(s) 816
EcoT22I ATGCAT 1 cut(s) 768
ErhI CCWWGG 1 cut(s) 816
FaeI CATG 4 cut(s) 218, 356, 618, 907
FalI AAGNNNNNCTT 2 cut(s) 170, 202
FaqI GGGAC 2 cut(s) 123, 363
FatI CATG 4 cut(s) 214, 352, 614, 903
FauI CCCGC 1 cut(s) 84
Fnu4HI GCNGC 1 cut(s) 836
FokI GGATG 2 cut(s) 459, 587
Fsp4HI GCNGC 1 cut(s) 836
FspBI CTAG 3 cut(s) 399, 681, 840
GlaI GCGC 1 cut(s) 1027
GluI GCNGC 1 cut(s) 836
GsuI CTGGAG 1 cut(s) 839
HaeIII GGCC 2 cut(s) 326, 838
HhaI GCGC 1 cut(s) 1028
Hin1II CATG 4 cut(s) 218, 356, 618, 907
Hin6I GCGC 1 cut(s) 1026
HinP1I GCGC 1 cut(s) 1026
HincII GTYRAC 1 cut(s) 283
HindII GTYRAC 1 cut(s) 283
HindIII AAGCTT 1 cut(s) 230
HinfI GANTC 4 cut(s) 455, 584, 594, 912
HphI GGTGA 3 cut(s) 62, 248, 907
Hpy166II GTNNAC 3 cut(s) 283, 781, 1021
Hpy188I TCNGA 5 cut(s) 132, 191, 228, 460, 717
Hpy188III TCNNGA 2 cut(s) 509, 661
Hpy8I GTNNAC 3 cut(s) 283, 781, 1021
HpyAV CCTTC 7 cut(s) 40, 100, 137, 386, 617, 660, 756
HpyCH4III ACNGT 1 cut(s) 422
HpyCH4V TGCA 7 cut(s) 70, 214, 306, 472, 618, 740, 766
HpyF10VI GCNNNNNNNGC 2 cut(s) 76, 395
HpyF3I CTNAG 2 cut(s) 114, 831
Hsp92II CATG 4 cut(s) 218, 356, 618, 907
HspAI GCGC 1 cut(s) 1026
Kzo9I GATC 1 cut(s) 464
LmnI GCTCC 1 cut(s) 395
LweI GCATC 1 cut(s) 481
MaeI CTAG 3 cut(s) 399, 681, 840
MaeIII GTNAC 3 cut(s) 50, 847, 962
MalI GATC 1 cut(s) 466
MboI GATC 1 cut(s) 464
MluCI AATT 6 cut(s) 206, 239, 272, 444, 531, 883
MluI ACGCGT 1 cut(s) 966
MlyI GAGTC 1 cut(s) 906
MmeI TCCRAC 2 cut(s) 179, 933
MnlI CCTC 5 cut(s) 37, 123, 340, 559, 565
Mph1103I ATGCAT 1 cut(s) 768
MseI TTAA 3 cut(s) 156, 377, 861
MslI CAYNNNNRTG 3 cut(s) 99, 219, 357
MspR9I CCNGG 4 cut(s) 137, 371, 552, 857
MvaI CCWGG 4 cut(s) 137, 371, 552, 857
MvnI CGCG 2 cut(s) 968, 1026
MwoI GCNNNNNNNGC 2 cut(s) 76, 395
NdeII GATC 1 cut(s) 464
NlaIII CATG 4 cut(s) 218, 356, 618, 907
NlaIV GGNNCC 3 cut(s) 111, 134, 759
NmuCI GTSAC 2 cut(s) 50, 847
NsiI ATGCAT 1 cut(s) 768
PaqCI CACCTGC 1 cut(s) 75
PcsI WCGNNNNNNNCGW 2 cut(s) 464, 1014
PfeI GAWTC 3 cut(s) 455, 584, 594
PflMI CCANNNNNTGG 1 cut(s) 924
PkrI GCNGC 1 cut(s) 837
PleI GAGTC 1 cut(s) 906
PpsI GAGTC 1 cut(s) 906
PpuMI RGGWCCY 1 cut(s) 110
Psp5II RGGWCCY 1 cut(s) 110
Psp6I CCWGG 4 cut(s) 135, 369, 550, 855
PspGI CCWGG 4 cut(s) 135, 369, 550, 855
PspN4I GGNNCC 3 cut(s) 111, 134, 759
PspPI GGNCC 3 cut(s) 110, 132, 647
PspPPI RGGWCCY 1 cut(s) 110
PstI CTGCAG 1 cut(s) 742
RsaI GTAC 1 cut(s) 685
RsaNI GTAC 1 cut(s) 684
RseI CAYNNNNRTG 3 cut(s) 99, 219, 357
SaqAI TTAA 3 cut(s) 156, 377, 861
SatI GCNGC 1 cut(s) 836
Sau3AI GATC 1 cut(s) 464
Sau96I GGNCC 3 cut(s) 110, 132, 647
ScaI AGTACT 1 cut(s) 685
SchI GAGTC 1 cut(s) 906
ScrFI CCNGG 4 cut(s) 137, 371, 552, 857
SfaNI GCATC 1 cut(s) 481
SfcI CTRYAG 1 cut(s) 738
SinI GGWCC 3 cut(s) 110, 132, 647
SmiMI CAYNNNNRTG 3 cut(s) 99, 219, 357
SmlI CTYRAG 1 cut(s) 509
SmoI CTYRAG 1 cut(s) 509
Sse9I AATT 6 cut(s) 206, 239, 272, 444, 531, 883
SsiI CCGC 4 cut(s) 77, 835, 929, 1024
SspMI CTAG 3 cut(s) 399, 681, 840
StyD4I CCNGG 4 cut(s) 135, 369, 550, 855
StyI CCWWGG 1 cut(s) 816
TaaI ACNGT 1 cut(s) 422
TaqI TCGA 3 cut(s) 253, 269, 1008
TasI AATT 6 cut(s) 206, 239, 272, 444, 531, 883
TatI WGTACW 1 cut(s) 683
TauI GCSGC 1 cut(s) 838
TfiI GAWTC 3 cut(s) 455, 584, 594
Tru1I TTAA 3 cut(s) 156, 377, 861
Tru9I TTAA 3 cut(s) 156, 377, 861
TseFI GTSAC 2 cut(s) 50, 847
Tsp45I GTSAC 2 cut(s) 50, 847
TspDTI ATGAA 6 cut(s) 375, 453, 555, 809, 909, 920
Van91I CCANNNNNTGG 1 cut(s) 924
VpaK11BI GGWCC 3 cut(s) 110, 132, 647
XapI RAATTY 3 cut(s) 206, 239, 272
XspI CTAG 3 cut(s) 399, 681, 840
ZrmI AGTACT 1 cut(s) 685
Zsp2I ATGCAT 1 cut(s) 768
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.