Rh2AG277000

B3 DNA binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0000593
Physical Location & Seq
Forward (+)
33248118 .. 33260192
12075 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG277000.1

Sequence Viewer

Length: 819 bp
ATGGCAAGAAAAAGAAAGCCTTCCTTTTTCAAGGTTCTTATAGGTGACTTCTCTGACAAGCTGAGAATCCCACAGGCTTTTCGTAAGCATTTGGCTGCAAGTGTACTTCAACAATCCCATCTTCGGACCCCTGCTGGAACTTGGCTTGTTGATGTGAAAAGCGATAACGGCAGGTTCTTTTTACAAAAGGGTTGGAAGCAATTTGTGCATGATAATGGTTTAAAGCTTGGAGAGTTTCTAATTTTCCGATATACTGGAAGTTCAAAATTCTATGTTGACATATATGGAAGACATGGCTGCAAAAAGGAGTTTGTAATGCCGGCTAGTAGTGAAAAACCATTTAAAAAGAGGCACGGTAATGAAAGTCATAGAACAAACGCTGTTATGAGTTCCAGCATACATCCAAAGAAGAAGATGGATTATCAGAAGTTAAAAGGTTCAGCTGGTGAAAATAAAACTAGTCAAGAAACTATTTCCGTCAAATCTGAACCAATTGATTTTGAATTGGAAACATCTATGGATATTGACGAGTCAATTAGAAATGTGAATTCTAGAGAGACTTTGTCTTTCCAACCTCAAGTGGCAAATAGTGCTCTAGAAGCAGCCAACAGATACTCATCGAACCATCCTTGCTTCAGAACTCTTTTGACCGAAACTTATGTGACCAAAGGAATTCTGGGAGGTTTTGAGATAAGATCTACACAAGGGTTAGCCTGTTCAACGCACGCGTTGTACATATTGAGGAACCAAAACACCTATATTTGGATCAACAAGATCAATCTGAGCAAGAAAGAAACCAGTATGACAAGTTTAAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

272

Amino Acids

31.02

Weight (kDa)

9.84

Isoelectric Point (pI)

36.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 9 - 96 6.3e-14 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 162
AccII CGCG 1 cut(s) 728
AclWI GGATC 1 cut(s) 773
AcsI RAATTY 3 cut(s) 266, 547, 672
AcuI CTGAAG 1 cut(s) 619
AfaI GTAC 2 cut(s) 105, 734
AfiI CCNNNNNNNGG 2 cut(s) 123, 762
AflIII ACRYGT 1 cut(s) 726
AgsI TTSAA 5 cut(s) 31, 110, 264, 503, 720
AhlI ACTAGT 1 cut(s) 458
AluBI AGCT 3 cut(s) 61, 226, 443
AluI AGCT 3 cut(s) 61, 226, 443
Alw21I GWGCWC 1 cut(s) 595
Alw26I GTCTC 1 cut(s) 551
AlwI GGATC 1 cut(s) 773
ApeKI GCWGC 3 cut(s) 95, 297, 602
ApoI RAATTY 3 cut(s) 266, 547, 672
Asp700I GAANNNNTTC 1 cut(s) 19
AspS9I GGNCC 1 cut(s) 126
AsuHPI GGTGA 2 cut(s) 56, 458
AvaII GGWCC 1 cut(s) 126
BbsI GAAGAC 1 cut(s) 295
Bbv12I GWGCWC 1 cut(s) 595
BbvI GCAGC 3 cut(s) 82, 284, 614
BccI CCATC 3 cut(s) 126, 409, 633
BceAI ACGGC 1 cut(s) 184
BcoDI GTCTC 1 cut(s) 551
BcuI ACTAGT 1 cut(s) 458
BfaI CTAG 4 cut(s) 324, 459, 552, 596
BfuAI ACCTGC 1 cut(s) 162
BglII AGATCT 1 cut(s) 695
BisI GCNGC 3 cut(s) 96, 298, 603
BlsI GCNGC 3 cut(s) 97, 299, 604
Bme18I GGWCC 1 cut(s) 126
BmgT120I GGNCC 1 cut(s) 126
BmiI GGNNCC 2 cut(s) 128, 746
BpiI GAAGAC 1 cut(s) 295
BpuEI CTTGAG 1 cut(s) 561
BsaBI GATNNNNATC 1 cut(s) 616
Bsc4I CCNNNNNNNGG 2 cut(s) 123, 762
Bse118I RCCGGY 1 cut(s) 319
Bse1I ACTGG 2 cut(s) 259, 798
Bse8I GATNNNNATC 1 cut(s) 616
BseGI GGATG 2 cut(s) 400, 625
BseJI GATNNNNATC 1 cut(s) 616
BseLI CCNNNNNNNGG 2 cut(s) 123, 762
BseMII CTCAG 2 cut(s) 53, 773
BseNI ACTGG 2 cut(s) 259, 798
BseXI GCAGC 3 cut(s) 82, 284, 614
Bsh1236I CGCG 1 cut(s) 728
BsiHKAI GWGCWC 1 cut(s) 595
BsiSI CCGG 1 cut(s) 320
BslI CCNNNNNNNGG 2 cut(s) 123, 762
BsmAI GTCTC 1 cut(s) 551
Bsp1286I GDGCHC 1 cut(s) 595
Bsp1407I TGTACA 1 cut(s) 732
Bsp143I GATC 3 cut(s) 695, 765, 774
BspCNI CTCAG 2 cut(s) 54, 774
BspFNI CGCG 1 cut(s) 728
BspLI GGNNCC 2 cut(s) 128, 746
BspMI ACCTGC 1 cut(s) 162
BspPI GGATC 1 cut(s) 773
BsrFI RCCGGY 1 cut(s) 319
BsrGI TGTACA 1 cut(s) 732
BsrI ACTGG 2 cut(s) 259, 798
BssAI RCCGGY 1 cut(s) 319
BssMI GATC 3 cut(s) 695, 765, 774
Bst4CI ACNGT 1 cut(s) 356
BstAPI GCANNNNNTGC 2 cut(s) 205, 590
BstAUI TGTACA 1 cut(s) 732
BstC8I GCNNGC 2 cut(s) 321, 726
BstDEI CTNAG 2 cut(s) 62, 782
BstF5I GGATG 2 cut(s) 400, 625
BstFNI CGCG 1 cut(s) 728
BstKTI GATC 3 cut(s) 698, 768, 777
BstMAI GTCTC 1 cut(s) 551
BstMBI GATC 3 cut(s) 695, 765, 774
BstMWI GCNNNNNNNGC 4 cut(s) 168, 205, 590, 599
BstUI CGCG 1 cut(s) 728
BstV1I GCAGC 3 cut(s) 82, 284, 614
BstV2I GAAGAC 1 cut(s) 295
BstX2I RGATCY 1 cut(s) 695
BstYI RGATCY 1 cut(s) 695
BtsCI GGATG 2 cut(s) 400, 625
BveI ACCTGC 1 cut(s) 162
Cac8I GCNNGC 2 cut(s) 321, 726
Cfr10I RCCGGY 1 cut(s) 319
Cfr13I GGNCC 1 cut(s) 126
Csp6I GTAC 2 cut(s) 104, 733
CviAII CATG 2 cut(s) 209, 293
CviQI GTAC 2 cut(s) 104, 733
DdeI CTNAG 2 cut(s) 62, 782
DpnI GATC 3 cut(s) 697, 767, 776
DpnII GATC 3 cut(s) 695, 765, 774
DraI TTTAAA 3 cut(s) 222, 343, 813
Eco47I GGWCC 1 cut(s) 126
Eco57I CTGAAG 1 cut(s) 619
EcoRI GAATTC 2 cut(s) 547, 672
FaeI CATG 2 cut(s) 212, 296
FalI AAGNNNNNCTT 3 cut(s) 36, 8, 40
FatI CATG 2 cut(s) 208, 292
Fnu4HI GCNGC 3 cut(s) 96, 298, 603
FokI GGATG 2 cut(s) 387, 612
Fsp4HI GCNGC 3 cut(s) 96, 298, 603
FspBI CTAG 4 cut(s) 324, 459, 552, 596
GluI GCNGC 3 cut(s) 96, 298, 603
HapII CCGG 1 cut(s) 320
Hin1II CATG 2 cut(s) 212, 296
HincII GTYRAC 1 cut(s) 277
HindII GTYRAC 1 cut(s) 277
HindIII AAGCTT 1 cut(s) 224
HinfI GANTC 2 cut(s) 66, 530
HpaII CCGG 1 cut(s) 320
HphI GGTGA 2 cut(s) 56, 458
Hpy166II GTNNAC 2 cut(s) 104, 277
Hpy188I TCNGA 7 cut(s) 55, 126, 248, 426, 487, 638, 783
Hpy188III TCNNGA 3 cut(s) 464, 552, 596
Hpy8I GTNNAC 2 cut(s) 104, 277
HpyAV CCTTC 1 cut(s) 30
HpyCH4III ACNGT 1 cut(s) 356
HpyCH4V TGCA 3 cut(s) 98, 208, 300
HpyF10VI GCNNNNNNNGC 4 cut(s) 168, 205, 590, 599
HpyF3I CTNAG 2 cut(s) 62, 782
Hsp92II CATG 2 cut(s) 212, 296
KroI GCCGGC 1 cut(s) 319
KroNI GCCGGC 1 cut(s) 321
Kzo9I GATC 3 cut(s) 695, 765, 774
Lsp1109I GCAGC 3 cut(s) 82, 284, 614
MaeI CTAG 4 cut(s) 324, 459, 552, 596
MaeIII GTNAC 2 cut(s) 44, 661
MalI GATC 3 cut(s) 697, 767, 776
MboI GATC 3 cut(s) 695, 765, 774
MboII GAAGA 4 cut(s) 113, 300, 421, 424
MfeI CAATTG 1 cut(s) 492
MflI RGATCY 1 cut(s) 695
MhlI GDGCHC 1 cut(s) 595
MluCI AATT 8 cut(s) 200, 240, 266, 492, 503, 534, 547, 672
MluI ACGCGT 1 cut(s) 726
MlyI GAGTC 1 cut(s) 539
MmeI TCCRAC 2 cut(s) 173, 595
MnlI CCTC 4 cut(s) 342, 585, 674, 735
MroNI GCCGGC 1 cut(s) 319
MroXI GAANNNNTTC 1 cut(s) 19
MseI TTAA 4 cut(s) 221, 342, 431, 812
MslI CAYNNNNRTG 2 cut(s) 213, 357
MspA1I CMGCKG 1 cut(s) 443
MspI CCGG 1 cut(s) 320
MunI CAATTG 1 cut(s) 492
MvnI CGCG 1 cut(s) 728
MwoI GCNNNNNNNGC 4 cut(s) 168, 205, 590, 599
NaeI GCCGGC 1 cut(s) 321
NdeII GATC 3 cut(s) 695, 765, 774
NgoMIV GCCGGC 1 cut(s) 319
NlaIII CATG 2 cut(s) 212, 296
NlaIV GGNNCC 2 cut(s) 128, 746
NmuCI GTSAC 2 cut(s) 44, 661
PdiI GCCGGC 1 cut(s) 321
PdmI GAANNNNTTC 1 cut(s) 19
PfeI GAWTC 1 cut(s) 66
PflFI GACNNNGTC 1 cut(s) 562
PkrI GCNGC 3 cut(s) 97, 299, 604
PleI GAGTC 1 cut(s) 538
PpsI GAGTC 1 cut(s) 538
PspN4I GGNNCC 2 cut(s) 128, 746
PspPI GGNCC 1 cut(s) 126
PsuI RGATCY 1 cut(s) 695
PsyI GACNNNGTC 1 cut(s) 562
PvuII CAGCTG 1 cut(s) 443
RsaI GTAC 2 cut(s) 105, 734
RsaNI GTAC 2 cut(s) 104, 733
RseI CAYNNNNRTG 2 cut(s) 213, 357
SaqAI TTAA 4 cut(s) 221, 342, 431, 812
SatI GCNGC 3 cut(s) 96, 298, 603
Sau3AI GATC 3 cut(s) 695, 765, 774
Sau96I GGNCC 1 cut(s) 126
SchI GAGTC 1 cut(s) 539
SduI GDGCHC 1 cut(s) 595
SinI GGWCC 1 cut(s) 126
SmiMI CAYNNNNRTG 2 cut(s) 213, 357
SmlI CTYRAG 1 cut(s) 576
SmoI CTYRAG 1 cut(s) 576
SpeI ACTAGT 1 cut(s) 458
Sse9I AATT 8 cut(s) 200, 240, 266, 492, 503, 534, 547, 672
SspMI CTAG 4 cut(s) 324, 459, 552, 596
TaaI ACNGT 1 cut(s) 356
TaqI TCGA 1 cut(s) 620
TaqII GACCGA 1 cut(s) 665
TasI AATT 8 cut(s) 200, 240, 266, 492, 503, 534, 547, 672
TatI WGTACW 2 cut(s) 103, 732
TfiI GAWTC 1 cut(s) 66
Tru1I TTAA 4 cut(s) 221, 342, 431, 812
Tru9I TTAA 4 cut(s) 221, 342, 431, 812
TseFI GTSAC 2 cut(s) 44, 661
TseI GCWGC 3 cut(s) 95, 297, 602
Tsp45I GTSAC 2 cut(s) 44, 661
TspDTI ATGAA 1 cut(s) 375
TspGWI ACGGA 1 cut(s) 466
Tth111I GACNNNGTC 1 cut(s) 562
VpaK11BI GGWCC 1 cut(s) 126
XapI RAATTY 3 cut(s) 266, 547, 672
XbaI TCTAGA 2 cut(s) 551, 595
XcmI CCANNNNNNNNNTGG 1 cut(s) 673
XmnI GAANNNNTTC 1 cut(s) 19
XspI CTAG 4 cut(s) 324, 459, 552, 596
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.