Rroxscaffold_2G00139650

B3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000593
Physical Location & Seq
Forward (+)
78022004 .. 78025066
3063 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00139650.1

Sequence Viewer

Length: 1206 bp
ATGGCAACAGAAAGAAACCCCTCGTTTTTCAAGGTTCTTCAGGGTGATCAGTTCTCTATCAAATTGAAAATCCCTAGTGCATTTCTCCAGTATTTTAATGGAAAGGTACCTCCCAAGTGCTTTCTTCAAACCCTAGGTCAAACTTGGCTTGTTGATGTGGAAAAGGTTAATCACAAGTTCTATTTCCAACTGGGGTGGAATCGCTTTGTGCAAGACAATGGCTTGAAGTATGGTCAACTTCTAGTCTTCCGTTATGCTGGAAACTCAGACTTCCATGTGGACATATTTGGAATAAATACCTGCAAAAATCTGTGTGTAATTCCTGAGATGGAAGAAGCTAATGAATATGATGATGATGTATCTCTTGAGATCTTGGACGACTTTCCACCCTGTCAAAGAAGAACAGAGGAGAAATCTTTGCTTTCATGTCCTCTGCCTCGCAAGAAAAACAAAACAAGTTCTAGTGGTAGAGCAGATATCACCCCTAACTTTCGAAAGAGGGTTCCACATCTTGAGAAGTCACAAATGAAAAGGAAAAAGGCACATTGGTTCAAATCAGAAATGATAGATAATTCTGATACTGATTCTGGTGATGAAGATGATCATGATGAGGAGGATGACGACGAAAATGAAGATGATGAAGAAGAAACTCAAGATGAAGAGGTTGAGGAGGAAGATGATGGTGATTCTGTTGGAACCCTCAACGATTCTCTACCATGCTCAAGAACAGTGGGGAAATCTACATTAGCATCGAGGCTGCACCAGAGAGAGAGAAAGAGTTCTAGTGCAAAAGCAAAAACCAACATCAAAACTGTTGATGGCTTGTCTAAAGAGGAAAAGATCCTAAAAGTTATAGCTCTACAAAGAGCTAATGCTTTCAAATCTAATTCTGTCAACCCTTGTTTCATGGTTTCCTTGTCTGCCACATATGCTCGTGGAGCTTTTCTGAATTTGCCACGGAGTTTTGCCGCAATACATTTTACTAATCCGCAGCCTGGCCACGTCATCCTTTGGGTTGAGAAGATCTCTTGGTTTGTCAAGGTCAGTTATATTGGGACTGCAACAAGATTACAGGCCGGCTGGTCTGAATTTGTGCATGTCAATAATTTGAAGATGGGTGATGCTTGTGTTTTTGTCTTGACGGATGCCATCAATTTTCAATATGATGTTGAAATATTCCGCGTTACAGATGCTGTTTGCACCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

401

Amino Acids

45.69

Weight (kDa)

5.29

Isoelectric Point (pI)

40.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 9 - 96 1.7e-13 B3 DNA binding domain
B3 PF02362 311 - 395 7.2e-12 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 308
Acc65I GGTACC 1 cut(s) 106
AccB1I GGYRCC 1 cut(s) 106
AccII CGCG 1 cut(s) 1182
AciI CCGC 3 cut(s) 969, 989, 1180
AclWI GGATC 1 cut(s) 835
AcoI YGGCCR 1 cut(s) 997
AcsI RAATTY 2 cut(s) 949, 1088
AcuI CTGAAG 1 cut(s) 23
AfaI GTAC 1 cut(s) 108
AfiI CCNNNNNNNGG 1 cut(s) 995
AgsI TTSAA 9 cut(s) 31, 67, 128, 226, 553, 880, 1111, 1160, 1172
AjiI CACGTC 1 cut(s) 1003
AjnI CCWGG 1 cut(s) 994
AluBI AGCT 4 cut(s) 338, 857, 869, 941
AluI AGCT 4 cut(s) 338, 857, 869, 941
AlwI GGATC 1 cut(s) 835
AlwNI CAGNNNCTG 1 cut(s) 1193
AoxI GGCC 2 cut(s) 997, 1074
ApeKI GCWGC 2 cut(s) 757, 991
ApoI RAATTY 2 cut(s) 949, 1088
ArsI GACNNNNNNTTYG 2 cut(s) 121, 153
Asp700I GAANNNNTTC 1 cut(s) 778
Asp718I GGTACC 1 cut(s) 106
AspA2I CCTAGG 1 cut(s) 133
AsuHPI GGTGA 5 cut(s) 56, 472, 602, 695, 1130
AsuII TTCGAA 1 cut(s) 493
AvrII CCTAGG 1 cut(s) 133
BalI TGGCCA 1 cut(s) 999
BanI GGYRCC 1 cut(s) 106
BauI CACGAG 1 cut(s) 933
BbsI GAAGAC 1 cut(s) 238
BbvI GCAGC 2 cut(s) 744, 1003
BccI CCATC 5 cut(s) 322, 674, 812, 1108, 1157
BciT130I CCWGG 1 cut(s) 996
BclI TGATCA 2 cut(s) 46, 601
BfaI CTAG 6 cut(s) 75, 134, 242, 462, 783, 1204
BfuAI ACCTGC 1 cut(s) 308
BglII AGATCT 2 cut(s) 369, 1023
BisI GCNGC 3 cut(s) 758, 969, 992
BlnI CCTAGG 1 cut(s) 133
BlsI GCNGC 3 cut(s) 759, 970, 993
Bme1390I CCNGG 1 cut(s) 996
BmgBI CACGTC 1 cut(s) 1003
BmiI GGNNCC 3 cut(s) 108, 504, 697
BmrFI CCNGG 1 cut(s) 996
BmrI ACTGGG 1 cut(s) 200
BmsI GCATC 4 cut(s) 758, 1111, 1135, 1180
BmuI ACTGGG 1 cut(s) 200
BpiI GAAGAC 1 cut(s) 238
BplI GAGNNNNNCTC 2 cut(s) 1010, 1042
BpmI CTGGAG 1 cut(s) 71
Bpu14I TTCGAA 1 cut(s) 493
BpuEI CTTGAG 4 cut(s) 386, 533, 636, 706
BsaJI CCNNGG 2 cut(s) 133, 956
Bsc4I CCNNNNNNNGG 1 cut(s) 995
Bse118I RCCGGY 1 cut(s) 1076
Bse1I ACTGG 2 cut(s) 88, 195
BseBI CCWGG 1 cut(s) 996
BseDI CCNNGG 2 cut(s) 133, 956
BseGI GGATG 3 cut(s) 622, 1005, 1150
BseLI CCNNNNNNNGG 1 cut(s) 995
BseMII CTCAG 2 cut(s) 279, 315
BseNI ACTGG 2 cut(s) 88, 195
BseRI GAGGAG 3 cut(s) 422, 626, 683
BseXI GCAGC 2 cut(s) 744, 1003
BsgI GTGCAG 1 cut(s) 743
Bsh1236I CGCG 1 cut(s) 1182
BshFI GGCC 2 cut(s) 999, 1076
BshNI GGYRCC 1 cut(s) 106
BsiSI CCGG 1 cut(s) 1077
BslFI GGGAC 1 cut(s) 1069
BslI CCNNNNNNNGG 1 cut(s) 995
BsmFI GGGAC 1 cut(s) 1069
BsnI GGCC 2 cut(s) 999, 1076
Bsp119I TTCGAA 1 cut(s) 493
Bsp143I GATC 5 cut(s) 46, 369, 601, 840, 1023
BspACI CCGC 3 cut(s) 969, 989, 1180
BspANI GGCC 2 cut(s) 999, 1076
BspCNI CTCAG 2 cut(s) 278, 316
BspFNI CGCG 1 cut(s) 1182
BspHI TCATGA 1 cut(s) 604
BspLI GGNNCC 3 cut(s) 108, 504, 697
BspMI ACCTGC 1 cut(s) 308
BspPI GGATC 1 cut(s) 835
BspT104I TTCGAA 1 cut(s) 493
BspT107I GGYRCC 1 cut(s) 106
BsrFI RCCGGY 1 cut(s) 1076
BsrI ACTGG 2 cut(s) 88, 195
BssAI RCCGGY 1 cut(s) 1076
BssECI CCNNGG 2 cut(s) 133, 956
BssMI GATC 5 cut(s) 46, 369, 601, 840, 1023
BssSI CACGAG 1 cut(s) 933
BssT1I CCWWGG 1 cut(s) 133
Bst2BI CACGAG 1 cut(s) 933
Bst2UI CCWGG 1 cut(s) 996
Bst4CI ACNGT 2 cut(s) 730, 814
Bst6I CTCTTC 1 cut(s) 654
BstBI TTCGAA 1 cut(s) 493
BstC8I GCNNGC 1 cut(s) 1078
BstDEI CTNAG 2 cut(s) 265, 324
BstDSI CCRYGG 1 cut(s) 956
BstF5I GGATG 3 cut(s) 622, 1005, 1150
BstFNI CGCG 1 cut(s) 1182
BstKTI GATC 5 cut(s) 49, 372, 604, 843, 1026
BstMBI GATC 5 cut(s) 46, 369, 601, 840, 1023
BstMWI GCNNNNNNNGC 2 cut(s) 929, 938
BstNI CCWGG 1 cut(s) 996
BstNSI RCATGY 1 cut(s) 1100
BstSCI CCNGG 1 cut(s) 994
BstUI CGCG 1 cut(s) 1182
BstV1I GCAGC 2 cut(s) 744, 1003
BstV2I GAAGAC 1 cut(s) 238
BstX2I RGATCY 3 cut(s) 369, 840, 1023
BstYI RGATCY 3 cut(s) 369, 840, 1023
BsuRI GGCC 2 cut(s) 999, 1076
BtgI CCRYGG 1 cut(s) 956
BtrI CACGTC 1 cut(s) 1003
BtsCI GGATG 3 cut(s) 622, 1005, 1150
BtsIMutI CAGTG 1 cut(s) 735
BveI ACCTGC 1 cut(s) 308
Cac8I GCNNGC 1 cut(s) 1078
CaiI CAGNNNCTG 1 cut(s) 1193
CciI TCATGA 1 cut(s) 604
Cfr10I RCCGGY 1 cut(s) 1076
Csp6I GTAC 1 cut(s) 107
CspCI CAANNNNNGTGG 4 cut(s) 176, 211, 711, 746
CviAII CATG 6 cut(s) 275, 426, 605, 717, 907, 1097
CviQI GTAC 1 cut(s) 107
DdeI CTNAG 2 cut(s) 265, 324
DpnI GATC 5 cut(s) 48, 371, 603, 842, 1025
DpnII GATC 5 cut(s) 46, 369, 601, 840, 1023
EaeI YGGCCR 1 cut(s) 997
Eam1104I CTCTTC 1 cut(s) 654
EarI CTCTTC 1 cut(s) 654
Eco130I CCWWGG 1 cut(s) 133
Eco32I GATATC 1 cut(s) 478
Eco57I CTGAAG 1 cut(s) 23
EcoRII CCWGG 1 cut(s) 994
EcoRV GATATC 1 cut(s) 478
EcoT14I CCWWGG 1 cut(s) 133
ErhI CCWWGG 1 cut(s) 133
FaeI CATG 6 cut(s) 278, 429, 608, 720, 910, 1100
FaqI GGGAC 1 cut(s) 1069
FatI CATG 6 cut(s) 274, 425, 604, 716, 906, 1096
FauNDI CATATG 1 cut(s) 928
FbaI TGATCA 2 cut(s) 46, 601
Fnu4HI GCNGC 3 cut(s) 758, 969, 992
FokI GGATG 3 cut(s) 629, 992, 1157
Fsp4HI GCNGC 3 cut(s) 758, 969, 992
FspBI CTAG 6 cut(s) 75, 134, 242, 462, 783, 1204
GluI GCNGC 3 cut(s) 758, 969, 992
GsuI CTGGAG 1 cut(s) 71
HaeIII GGCC 2 cut(s) 999, 1076
HapII CCGG 1 cut(s) 1077
Hin1II CATG 6 cut(s) 278, 429, 608, 720, 910, 1100
HincII GTYRAC 2 cut(s) 236, 895
HindII GTYRAC 2 cut(s) 236, 895
HinfI GANTC 4 cut(s) 199, 584, 686, 707
HpaII CCGG 1 cut(s) 1077
HphI GGTGA 5 cut(s) 56, 472, 602, 695, 1130
Hpy166II GTNNAC 3 cut(s) 236, 280, 895
Hpy188I TCNGA 5 cut(s) 268, 559, 577, 948, 1087
Hpy188III TCNNGA 7 cut(s) 323, 365, 512, 605, 653, 723, 1138
Hpy8I GTNNAC 3 cut(s) 236, 280, 895
Hpy99I CGWCG 1 cut(s) 626
HpyCH4III ACNGT 2 cut(s) 730, 814
HpyCH4IV ACGT 1 cut(s) 1002
HpyCH4V TGCA 8 cut(s) 80, 211, 303, 760, 788, 1061, 1096, 1200
HpyF10VI GCNNNNNNNGC 2 cut(s) 929, 938
HpyF3I CTNAG 2 cut(s) 265, 324
HpySE526I ACGT 1 cut(s) 1002
Hsp92II CATG 6 cut(s) 278, 429, 608, 720, 910, 1100
KpnI GGTACC 1 cut(s) 110
KroI GCCGGC 1 cut(s) 1076
KroNI GCCGGC 1 cut(s) 1078
Ksp22I TGATCA 2 cut(s) 46, 601
Kzo9I GATC 5 cut(s) 46, 369, 601, 840, 1023
LmnI GCTCC 1 cut(s) 938
Lsp1109I GCAGC 2 cut(s) 744, 1003
LweI GCATC 4 cut(s) 758, 1111, 1135, 1180
MaeI CTAG 6 cut(s) 75, 134, 242, 462, 783, 1204
MaeII ACGT 1 cut(s) 1002
MaeIII GTNAC 2 cut(s) 519, 1183
MalI GATC 5 cut(s) 48, 371, 603, 842, 1025
MboI GATC 5 cut(s) 46, 369, 601, 840, 1023
MflI RGATCY 3 cut(s) 369, 840, 1023
MlsI TGGCCA 1 cut(s) 999
MluCI AATT 8 cut(s) 62, 318, 571, 886, 949, 1088, 1105, 1153
MluNI TGGCCA 1 cut(s) 999
MmeI TCCRAC 2 cut(s) 211, 673
Mox20I TGGCCA 1 cut(s) 999
MroNI GCCGGC 1 cut(s) 1076
MroXI GAANNNNTTC 1 cut(s) 778
MscI TGGCCA 1 cut(s) 999
MseI TTAA 2 cut(s) 96, 168
Msp20I TGGCCA 1 cut(s) 999
MspI CCGG 1 cut(s) 1077
MspR9I CCNGG 1 cut(s) 996
MvaI CCWGG 1 cut(s) 996
MvnI CGCG 1 cut(s) 1182
MwoI GCNNNNNNNGC 2 cut(s) 929, 938
NaeI GCCGGC 1 cut(s) 1078
NdeI CATATG 1 cut(s) 928
NdeII GATC 5 cut(s) 46, 369, 601, 840, 1023
NgoMIV GCCGGC 1 cut(s) 1076
NlaIII CATG 6 cut(s) 278, 429, 608, 720, 910, 1100
NlaIV GGNNCC 3 cut(s) 108, 504, 697
NmuCI GTSAC 1 cut(s) 519
NspI RCATGY 1 cut(s) 1100
NspV TTCGAA 1 cut(s) 493
PagI TCATGA 1 cut(s) 604
PdiI GCCGGC 1 cut(s) 1078
PdmI GAANNNNTTC 1 cut(s) 778
PfeI GAWTC 4 cut(s) 199, 584, 686, 707
PkrI GCNGC 3 cut(s) 759, 970, 993
Psp6I CCWGG 1 cut(s) 994
PspGI CCWGG 1 cut(s) 994
PspN4I GGNNCC 3 cut(s) 108, 504, 697
PstNI CAGNNNCTG 1 cut(s) 1193
PsuI RGATCY 3 cut(s) 369, 840, 1023
RsaI GTAC 1 cut(s) 108
RsaNI GTAC 1 cut(s) 107
SaqAI TTAA 2 cut(s) 96, 168
SatI GCNGC 3 cut(s) 758, 969, 992
Sau3AI GATC 5 cut(s) 46, 369, 601, 840, 1023
ScrFI CCNGG 1 cut(s) 996
SfaNI GCATC 4 cut(s) 758, 1111, 1135, 1180
SfuI TTCGAA 1 cut(s) 493
SmlI CTYRAG 4 cut(s) 365, 512, 651, 721
SmoI CTYRAG 4 cut(s) 365, 512, 651, 721
Sse9I AATT 8 cut(s) 62, 318, 571, 886, 949, 1088, 1105, 1153
SsiI CCGC 3 cut(s) 969, 989, 1180
SspI AATATT 1 cut(s) 1176
SspMI CTAG 6 cut(s) 75, 134, 242, 462, 783, 1204
StyD4I CCNGG 1 cut(s) 994
StyI CCWWGG 1 cut(s) 133
TaaI ACNGT 2 cut(s) 730, 814
TaiI ACGT 1 cut(s) 1005
TaqI TCGA 2 cut(s) 493, 752
TasI AATT 8 cut(s) 62, 318, 571, 886, 949, 1088, 1105, 1153
TauI GCSGC 1 cut(s) 971
TfiI GAWTC 4 cut(s) 199, 584, 686, 707
Tru1I TTAA 2 cut(s) 96, 168
Tru9I TTAA 2 cut(s) 96, 168
TscAI CASTG 1 cut(s) 735
TseFI GTSAC 1 cut(s) 519
TseI GCWGC 2 cut(s) 757, 991
Tsp45I GTSAC 1 cut(s) 519
TspDTI ATGAA 8 cut(s) 357, 414, 542, 609, 645, 654, 672, 895
TspGWI ACGGA 3 cut(s) 239, 973, 1157
TspRI CASTG 1 cut(s) 735
XapI RAATTY 2 cut(s) 949, 1088
XceI RCATGY 1 cut(s) 1100
XcmI CCANNNNNNNNNTGG 1 cut(s) 95
XmaJI CCTAGG 1 cut(s) 133
XmnI GAANNNNTTC 1 cut(s) 778
XspI CTAG 6 cut(s) 75, 134, 242, 462, 783, 1204
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.