Rorug02G0114800

B3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0000593
Physical Location & Seq
Forward (+)
9795428 .. 9796660
1233 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0114800.1

Sequence Viewer

Length: 1233 bp
ATGGAGAGCGTGAACTGGACCGATCTTCCAAAGGAGCTCTGGCTAATCATCACTGAATGCTTCCAAAGCCGTATCGGAATTCTCCGATTCCGCAGCGTCTGCTCCTCATGGCATTCCTCTCTCCCTCCTTCCGAGCAATTTTCCCCTCCTCCTTTCCCCCTCAAGTTCTCCTCCCCCGGCGAAGACTCTCCCGGAAACCATGCTTCATTGTTCCAATCAACAATCTACCGCATGGAGTCATGCATTGACGAGGATAACTGCGGTTCTTCACCATCAAACCTTTGTTCGAAGCCATGGTTGGTGAAGTTTGAGGAGGACCCTAGTTCTGGTATAATGCGTTTACTAGATCCGGTGACTAGTTCGCCTCTCAGGTATTCTCCCAATACTTCTTTAAAGGAATTAAACTTATTGGAGTATCGGATTGTAGAGCTACACAAGTCGTATGGACTTAAATTTGGCGAGAACAATGTTACTATTAATTCTGTGCACAAATTGGTAGTCCTCCCTCGTAACAATTTGGATTTTGGTGATGTGTGTGCGGTTTTTATGATTTATAATGAAGGCAAGCTGGGTTTTATGAGATTTGGAGATGAGGAGTTGACCCATGTGGATGAGGAAAACTCCTACTATGATGATATTATTGTGTACAAGGGTCAATGCTATGTGGTTGATAAGTGGGGAACGATTTCCTCTATCAATTCAGCTCTGGAGGTGATCCAGTTTTCGCCTCTATTGTCGTGTGGATTTGGTGGGCAGAAGCATTTGGTTGAGTCTTGTGGTGATCTTTATGTGGTTGATCAGTACTATGAGAGGGTGAGTGTTCGTCACGGCAGGGAGGACGAGTACATTTTCGGGAAGTATCACAATTCGCGCTATGATTCTGATGCAATCGATTTTAAAGTTTACAAGCTGGATCAAGAATGGAGTAAATGGGACAATGTGAAAAACTTGGGTGATCATGTGTTTCTTCTGAGCAAAGATGGGTCTTTCTCTGTCTCCACCAGAGAGTTTGATGGAGTGAAGGGAAATTGCATCTTCTTCATTGAACGAGTGTCGCCTACTGTAAGAGCAAGGTATCACAGTCGTGTGTTCGATTTAGAGGATGGTTCGATGAGGGACTTAGCATCTTTGCCGATTTCTCGGATGGTCGAGCCACCTCCAACTTGGCTCAGCCCTGATTTGAGTTCTTCTGCATCTGAATGTGAGTGTGAAATATTAATGCCAATTCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

410

Amino Acids

46.77

Weight (kDa)

4.94

Isoelectric Point (pI)

50.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_KIB1-4 PF03478 95 - 357 8.5e-37 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 555
AccII CGCG 1 cut(s) 871
AciI CCGC 4 cut(s) 91, 229, 261, 539
AclWI GGATC 3 cut(s) 341, 709, 921
AcsI RAATTY 2 cut(s) 78, 452
AfaI GTAC 3 cut(s) 647, 803, 845
AfiI CCNNNNNNNGG 1 cut(s) 326
AgsI TTSAA 1 cut(s) 1046
AhlI ACTAGT 1 cut(s) 356
AjuI GAANNNNNNNTTGG 2 cut(s) 281, 313
AleI CACNNNNGTG 1 cut(s) 1083
AluBI AGCT 5 cut(s) 37, 430, 568, 704, 910
AluI AGCT 5 cut(s) 37, 430, 568, 704, 910
Alw21I GWGCWC 2 cut(s) 39, 489
Alw26I GTCTC 1 cut(s) 1000
Alw44I GTGCAC 1 cut(s) 485
AlwI GGATC 3 cut(s) 341, 709, 921
AlwNI CAGNNNCTG 1 cut(s) 99
ApaLI GTGCAC 1 cut(s) 485
ApeKI GCWGC 1 cut(s) 93
ApoI RAATTY 2 cut(s) 78, 452
ArsI GACNNNNNNTTYG 2 cut(s) 483, 515
AseI ATTAAT 2 cut(s) 477, 1217
Asp700I GAANNNNTTC 1 cut(s) 685
AspLEI GCGC 1 cut(s) 873
AspS9I GGNCC 2 cut(s) 18, 316
AsuC2I CCSGG 2 cut(s) 177, 192
AsuHPI GGTGA 8 cut(s) 261, 313, 364, 539, 724, 791, 826, 965
AsuII TTCGAA 1 cut(s) 287
AvaII GGWCC 2 cut(s) 18, 316
BaeGI GKGCMC 1 cut(s) 489
BanII GRGCYC 1 cut(s) 39
BbsI GAAGAC 1 cut(s) 189
Bbv12I GWGCWC 2 cut(s) 39, 489
BbvI GCAGC 1 cut(s) 105
BccI CCATC 5 cut(s) 280, 974, 1007, 1097, 1138
BceAI ACGGC 2 cut(s) 54, 844
BclI TGATCA 2 cut(s) 796, 955
BcnI CCSGG 2 cut(s) 177, 192
BcoDI GTCTC 1 cut(s) 1000
BcuI ACTAGT 1 cut(s) 356
BfaI CTAG 3 cut(s) 321, 344, 357
BisI GCNGC 1 cut(s) 94
BlpI GCTNAGC 1 cut(s) 1169
BlsI GCNGC 1 cut(s) 95
BmcAI AGTACT 1 cut(s) 803
Bme1390I CCNGG 2 cut(s) 177, 192
Bme18I GGWCC 2 cut(s) 18, 316
BmgT120I GGNCC 2 cut(s) 18, 316
BmiI GGNNCC 1 cut(s) 318
BmrFI CCNGG 2 cut(s) 177, 192
BmsI GCATC 4 cut(s) 874, 1041, 1133, 1202
BpiI GAAGAC 1 cut(s) 189
BplI GAGNNNNNCTC 2 cut(s) 605, 637
BpmI CTGGAG 1 cut(s) 728
Bpu1102I GCTNAGC 1 cut(s) 1169
Bpu14I TTCGAA 1 cut(s) 287
BpuEI CTTGAG 1 cut(s) 146
BpuMI CCSGG 2 cut(s) 177, 192
Bsa29I ATCGAT 1 cut(s) 891
BsaJI CCNNGG 2 cut(s) 175, 293
BsaWI WCCGGW 1 cut(s) 349
BsaXI ACNNNNNCTCC 2 cut(s) 827, 857
Bsc4I CCNNNNNNNGG 1 cut(s) 326
Bse1I ACTGG 2 cut(s) 20, 718
BseCI ATCGAT 1 cut(s) 891
BseDI CCNNGG 2 cut(s) 175, 293
BseGI GGATG 3 cut(s) 616, 1108, 1149
BseLI CCNNNNNNNGG 1 cut(s) 326
BseMII CTCAG 3 cut(s) 382, 962, 1183
BseNI ACTGG 2 cut(s) 20, 718
BseRI GAGGAG 5 cut(s) 94, 138, 160, 326, 608
BseSI GKGCMC 1 cut(s) 489
BseXI GCAGC 1 cut(s) 105
BseYI CCCAGC 1 cut(s) 568
Bsh1236I CGCG 1 cut(s) 871
BshVI ATCGAT 1 cut(s) 891
BsiHKAI GWGCWC 2 cut(s) 39, 489
BsiSI CCGG 3 cut(s) 177, 192, 350
BslFI GGGAC 2 cut(s) 947, 1130
BslI CCNNNNNNNGG 1 cut(s) 326
BsmAI GTCTC 1 cut(s) 1000
BsmFI GGGAC 2 cut(s) 947, 1130
BsmI GAATGC 2 cut(s) 62, 112
Bsp119I TTCGAA 1 cut(s) 287
Bsp1286I GDGCHC 2 cut(s) 39, 489
Bsp1407I TGTACA 1 cut(s) 645
Bsp143I GATC 7 cut(s) 22, 346, 714, 781, 796, 913, 955
Bsp1720I GCTNAGC 1 cut(s) 1169
Bsp19I CCATGG 1 cut(s) 293
BspACI CCGC 4 cut(s) 91, 229, 261, 539
BspCNI CTCAG 3 cut(s) 381, 963, 1182
BspDI ATCGAT 1 cut(s) 891
BspFNI CGCG 1 cut(s) 871
BspLI GGNNCC 1 cut(s) 318
BspPI GGATC 3 cut(s) 341, 709, 921
BspT104I TTCGAA 1 cut(s) 287
BsrGI TGTACA 1 cut(s) 645
BsrI ACTGG 2 cut(s) 20, 718
BssECI CCNNGG 2 cut(s) 175, 293
BssMI GATC 7 cut(s) 22, 346, 714, 781, 796, 913, 955
BssT1I CCWWGG 1 cut(s) 293
Bst4CI ACNGT 2 cut(s) 1063, 1082
BstAPI GCANNNNNTGC 1 cut(s) 99
BstAUI TGTACA 1 cut(s) 645
BstBI TTCGAA 1 cut(s) 287
BstC8I GCNNGC 1 cut(s) 566
BstDEI CTNAG 4 cut(s) 368, 971, 1120, 1169
BstDSI CCRYGG 1 cut(s) 293
BstF5I GGATG 3 cut(s) 616, 1108, 1149
BstFNI CGCG 1 cut(s) 871
BstHHI GCGC 1 cut(s) 873
BstKTI GATC 7 cut(s) 25, 349, 717, 784, 799, 916, 958
BstMAI GTCTC 1 cut(s) 1000
BstMBI GATC 7 cut(s) 22, 346, 714, 781, 796, 913, 955
BstMWI GCNNNNNNNGC 2 cut(s) 66, 99
BstSCI CCNGG 2 cut(s) 175, 190
BstSLI GKGCMC 1 cut(s) 489
BstUI CGCG 1 cut(s) 871
BstV1I GCAGC 1 cut(s) 105
BstV2I GAAGAC 1 cut(s) 189
BstX2I RGATCY 1 cut(s) 346
BstYI RGATCY 1 cut(s) 346
Bsu15I ATCGAT 1 cut(s) 891
BsuTUI ATCGAT 1 cut(s) 891
BtgI CCRYGG 1 cut(s) 293
BtsCI GGATG 3 cut(s) 616, 1108, 1149
BtsIMutI CAGTG 1 cut(s) 51
Cac8I GCNNGC 1 cut(s) 566
CaiI CAGNNNCTG 1 cut(s) 99
CfoI GCGC 1 cut(s) 873
Cfr13I GGNCC 2 cut(s) 18, 316
ClaI ATCGAT 1 cut(s) 891
CseI GACGC 1 cut(s) 85
Csp6I GTAC 3 cut(s) 646, 802, 844
CviAII CATG 7 cut(s) 108, 200, 232, 240, 294, 605, 959
CviQI GTAC 3 cut(s) 646, 802, 844
DdeI CTNAG 4 cut(s) 368, 971, 1120, 1169
DpnI GATC 7 cut(s) 24, 348, 716, 783, 798, 915, 957
DpnII GATC 7 cut(s) 22, 346, 714, 781, 796, 913, 955
DraI TTTAAA 2 cut(s) 393, 898
Ecl136II GAGCTC 1 cut(s) 37
Eco130I CCWWGG 1 cut(s) 293
Eco24I GRGCYC 1 cut(s) 39
Eco47I GGWCC 2 cut(s) 18, 316
Eco53kI GAGCTC 1 cut(s) 37
EcoICRI GAGCTC 1 cut(s) 37
EcoO109I RGGNCCY 1 cut(s) 316
EcoRI GAATTC 1 cut(s) 78
EcoT14I CCWWGG 1 cut(s) 293
EcoT22I ATGCAT 1 cut(s) 245
EcoT38I GRGCYC 1 cut(s) 39
ErhI CCWWGG 1 cut(s) 293
FaeI CATG 7 cut(s) 111, 203, 235, 243, 297, 608, 962
FaqI GGGAC 2 cut(s) 947, 1130
FatI CATG 7 cut(s) 107, 199, 231, 239, 293, 604, 958
FbaI TGATCA 2 cut(s) 796, 955
Fnu4HI GCNGC 1 cut(s) 94
FokI GGATG 3 cut(s) 623, 1115, 1156
FriOI GRGCYC 1 cut(s) 39
Fsp4HI GCNGC 1 cut(s) 94
FspBI CTAG 3 cut(s) 321, 344, 357
GlaI GCGC 1 cut(s) 872
GluI GCNGC 1 cut(s) 94
GsaI CCCAGC 1 cut(s) 572
GsuI CTGGAG 1 cut(s) 728
HapII CCGG 3 cut(s) 177, 192, 350
HgaI GACGC 1 cut(s) 85
HhaI GCGC 1 cut(s) 873
Hin1II CATG 7 cut(s) 111, 203, 235, 243, 297, 608, 962
Hin6I GCGC 1 cut(s) 871
HinP1I GCGC 1 cut(s) 871
HincII GTYRAC 1 cut(s) 600
HindII GTYRAC 1 cut(s) 600
HinfI GANTC 5 cut(s) 87, 185, 236, 770, 878
HpaII CCGG 3 cut(s) 177, 192, 350
HphI GGTGA 8 cut(s) 261, 313, 364, 539, 724, 791, 826, 965
Hpy166II GTNNAC 6 cut(s) 13, 341, 487, 600, 646, 904
Hpy188I TCNGA 8 cut(s) 77, 86, 133, 420, 883, 972, 1143, 1198
Hpy188III TCNNGA 3 cut(s) 707, 853, 917
Hpy8I GTNNAC 6 cut(s) 13, 341, 487, 600, 646, 904
HpyAV CCTTC 3 cut(s) 138, 554, 1015
HpyCH4III ACNGT 2 cut(s) 1063, 1082
HpyCH4V TGCA 5 cut(s) 243, 487, 887, 1032, 1193
HpyF10VI GCNNNNNNNGC 2 cut(s) 66, 99
HpyF3I CTNAG 4 cut(s) 368, 971, 1120, 1169
Hsp92II CATG 7 cut(s) 111, 203, 235, 243, 297, 608, 962
HspAI GCGC 1 cut(s) 871
Ksp22I TGATCA 2 cut(s) 796, 955
Kzo9I GATC 7 cut(s) 22, 346, 714, 781, 796, 913, 955
LmnI GCTCC 2 cut(s) 34, 107
Lsp1109I GCAGC 1 cut(s) 105
LweI GCATC 4 cut(s) 874, 1041, 1133, 1202
MaeI CTAG 3 cut(s) 321, 344, 357
MaeIII GTNAC 4 cut(s) 352, 469, 509, 824
MalI GATC 7 cut(s) 24, 348, 716, 783, 798, 915, 957
MboI GATC 7 cut(s) 22, 346, 714, 781, 796, 913, 955
MboII GAAGA 7 cut(s) 17, 194, 258, 959, 1027, 1030, 1179
MflI RGATCY 1 cut(s) 346
MhlI GDGCHC 2 cut(s) 39, 489
MlyI GAGTC 3 cut(s) 179, 245, 779
MmeI TCCRAC 1 cut(s) 1184
Mph1103I ATGCAT 1 cut(s) 245
MroXI GAANNNNTTC 1 cut(s) 685
MseI TTAA 6 cut(s) 392, 401, 450, 477, 897, 1217
MslI CAYNNNNRTG 3 cut(s) 609, 1083, 1198
MspI CCGG 3 cut(s) 177, 192, 350
MspR9I CCNGG 2 cut(s) 177, 192
Mva1269I GAATGC 2 cut(s) 62, 112
MvnI CGCG 1 cut(s) 871
MwoI GCNNNNNNNGC 2 cut(s) 66, 99
NciI CCSGG 2 cut(s) 177, 192
NcoI CCATGG 1 cut(s) 293
NdeII GATC 7 cut(s) 22, 346, 714, 781, 796, 913, 955
NlaIII CATG 7 cut(s) 111, 203, 235, 243, 297, 608, 962
NlaIV GGNNCC 1 cut(s) 318
NmuCI GTSAC 2 cut(s) 352, 824
NsiI ATGCAT 1 cut(s) 245
NspV TTCGAA 1 cut(s) 287
OliI CACNNNNGTG 1 cut(s) 1083
PctI GAATGC 2 cut(s) 62, 112
PdmI GAANNNNTTC 1 cut(s) 685
PfeI GAWTC 2 cut(s) 87, 878
PfoI TCCNGGA 1 cut(s) 190
PkrI GCNGC 1 cut(s) 95
PleI GAGTC 3 cut(s) 179, 244, 778
PpsI GAGTC 3 cut(s) 179, 244, 778
PpuMI RGGWCCY 1 cut(s) 316
PshBI ATTAAT 2 cut(s) 477, 1217
PsiI TTATAA 1 cut(s) 555
Psp124BI GAGCTC 1 cut(s) 39
Psp5II RGGWCCY 1 cut(s) 316
PspFI CCCAGC 1 cut(s) 568
PspN4I GGNNCC 1 cut(s) 318
PspPI GGNCC 2 cut(s) 18, 316
PspPPI RGGWCCY 1 cut(s) 316
PstNI CAGNNNCTG 1 cut(s) 99
PsuI RGATCY 1 cut(s) 346
RsaI GTAC 3 cut(s) 647, 803, 845
RsaNI GTAC 3 cut(s) 646, 802, 844
RseI CAYNNNNRTG 3 cut(s) 609, 1083, 1198
SacI GAGCTC 1 cut(s) 39
SaqAI TTAA 6 cut(s) 392, 401, 450, 477, 897, 1217
SatI GCNGC 1 cut(s) 94
Sau3AI GATC 7 cut(s) 22, 346, 714, 781, 796, 913, 955
Sau96I GGNCC 2 cut(s) 18, 316
ScaI AGTACT 1 cut(s) 803
SchI GAGTC 3 cut(s) 179, 245, 779
ScrFI CCNGG 2 cut(s) 177, 192
SduI GDGCHC 2 cut(s) 39, 489
SfaNI GCATC 4 cut(s) 874, 1041, 1133, 1202
SfuI TTCGAA 1 cut(s) 287
SinI GGWCC 2 cut(s) 18, 316
SmiMI CAYNNNNRTG 3 cut(s) 609, 1083, 1198
SmlI CTYRAG 1 cut(s) 161
SmoI CTYRAG 1 cut(s) 161
SpeI ACTAGT 1 cut(s) 356
SsiI CCGC 4 cut(s) 91, 229, 261, 539
SspI AATATT 1 cut(s) 1215
SspMI CTAG 3 cut(s) 321, 344, 357
SstI GAGCTC 1 cut(s) 39
StyD4I CCNGG 2 cut(s) 175, 190
StyI CCWWGG 1 cut(s) 293
TaaI ACNGT 2 cut(s) 1063, 1082
TaqI TCGA 5 cut(s) 287, 891, 1092, 1109, 1149
TaqII GACCGA 1 cut(s) 35
TatI WGTACW 3 cut(s) 645, 801, 843
TfiI GAWTC 2 cut(s) 87, 878
Tru1I TTAA 6 cut(s) 392, 401, 450, 477, 897, 1217
Tru9I TTAA 6 cut(s) 392, 401, 450, 477, 897, 1217
TscAI CASTG 1 cut(s) 58
TseFI GTSAC 2 cut(s) 352, 824
TseI GCWGC 1 cut(s) 93
Tsp45I GTSAC 2 cut(s) 352, 824
TspDTI ATGAA 3 cut(s) 195, 573, 1030
TspRI CASTG 1 cut(s) 58
VneI GTGCAC 1 cut(s) 485
VpaK11BI GGWCC 2 cut(s) 18, 316
VspI ATTAAT 2 cut(s) 477, 1217
XapI RAATTY 2 cut(s) 78, 452
XcmI CCANNNNNNNNNTGG 2 cut(s) 36, 1161
XmnI GAANNNNTTC 1 cut(s) 685
XspI CTAG 3 cut(s) 321, 344, 357
ZrmI AGTACT 1 cut(s) 803
Zsp2I ATGCAT 1 cut(s) 245
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.