Rw2G012800

B3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0000593
Physical Location & Seq
Reverse (-)
13390447 .. 13393187
2741 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G012800.1

Sequence Viewer

Length: 1443 bp
ATGGCAACAACAAGAAAACCCTCTTTCTTCAAGGTTCTTATAGGGGATCACTTCTGTAACAGATTGAAAATTCCGGGAGATTTTCTTGAGCATTTTGATGGGAGAGTACCTGAACAGTGCCATCTTAGAATCGGCACTCAAACTTGGCTTGTTGATGTTGAAATGGTCAATCAGAAGTTCTATTTCCAAAATGGTTGGAAGGAATTTGTGCTTGAGAATGATTTAAGGGGTTGCGAGTTTCTAGTTTTTTTCTATAATGGAAATTCGGAGTTCTATGTCCACATATATGGGAAGAATGCTTGCAGAAAGGAGTTTGCAGATGCCGCAGCTGGAGTAAGTGGCACACCGAAATCAATGCATGTTGGTAGCTCCAGAGGGATGGAAGAAGCTGATAGTAATGACTCATCTGTTCAGATCTTGGATGACTTTCCAATCTGCTCAAGAAGAGCAGGGGAGAAATCTTCATTACCAAGTCATCAGCAGCACAAGAAAGACAGTACAAGCTGGATTGGTAAAGAGACACATGGTCCCAAACCAGGAATTACAAATAATGATTTTCCAGCGGAACAATATGTGGGAGGGACTTCTAGCTCGAAACCAAAACATGAGGTTCTTGGGAGCTGGATGCACACTGATGATGACGATGATGACAAAGAAGAGGAAGAGAGTGAGGAGGATGATGATGATTATGAGGAAGATGAAGAGAGTGAGGAGGATGAAAATGAAGCTGACAGTGAAGACAAAGATGGGTATGAAGATGAAGAAGAGGAAGAAGAAAGCGATGGAGATGGGGGAAAAAGTGATGATTCTATTGAAATTCTGGACGAGTTTCCACGATGCTCAAAAGCAAAAACAATGAGGAAATCATCAGGGCTTCAGAAGAGAAAGATAAACAGTTCTAAAGCTAAAACAAAAAGAAGCATAACACGTGCTAGTGGCTCGTCTAGCAATCAAAAAATCACAGGATGGAAATATAAGGTTTTAAGGAAAATGCCTGGATCGAATGGAATTGGAGAACTTATAGCCCGTCACAGAGCTAAAGATTTCATATCCAAATCTGACAAGCTGGATTCTTGTTTGATTGCCATGTCTCCTTCGTATATTAGAGGATCTTGTGTGCATTTTCCACGTACTTTTGCCTACAGACATCTTGGAAGGCATTGTAGTCGCATCAAACTTCGGGTTAATAAGAGGAGGTGGCGTCTTGGTATCTATCATGTCCATAAAACAGTTAGATTACAAGCTGGATGGATTGAATTTAAGCATGACAATAATCTGAAAGTGGGTGATGTGTGCGTCTTTGAGTTGACTGACAGCATCAATGTACTTGATGTTACAATTTTCCGCGCCGCCACAGAAGCTGCAAATTGCACCTTGCCAGGGGGAAGACAAATGACTGGGGTTCAATTCAAGTCGGAAAAAGGAAAAGAGAAAGCTGGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

480

Amino Acids

54.35

Weight (kDa)

5.78

Isoelectric Point (pI)

54.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 9 - 98 1.1e-10 B3 DNA binding domain
B3 PF02362 364 - 450 1.4e-10 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 1347
AciI CCGC 4 cut(s) 324, 563, 1345, 1350
AclWI GGATC 3 cut(s) 54, 1006, 1117
AcsI RAATTY 5 cut(s) 69, 203, 262, 816, 1256
AcuI CTGAAG 1 cut(s) 860
AcvI CACGTG 1 cut(s) 929
AcyI GRCGYC 1 cut(s) 1201
AfaI GTAC 4 cut(s) 108, 499, 1132, 1326
AfiI CCNNNNNNNGG 2 cut(s) 536, 1380
AflIII ACRYGT 1 cut(s) 926
AgsI TTSAA 7 cut(s) 31, 67, 161, 815, 1256, 1406, 1411
AhdI GACNNNNNGTC 1 cut(s) 525
AjnI CCWGG 3 cut(s) 535, 994, 1378
Alw26I GTCTC 2 cut(s) 512, 1095
AlwI GGATC 3 cut(s) 54, 1006, 1117
AlwNI CAGNNNCTG 1 cut(s) 1361
ApeKI GCWGC 3 cut(s) 326, 481, 1361
ApoI RAATTY 5 cut(s) 69, 203, 262, 816, 1256
AspLEI GCGC 1 cut(s) 1349
AspS9I GGNCC 1 cut(s) 527
AsuC2I CCSGG 1 cut(s) 75
AsuHPI GGTGA 1 cut(s) 1298
AvaII GGWCC 1 cut(s) 527
BbrPI CACGTG 1 cut(s) 929
BbsI GAAGAC 2 cut(s) 744, 1393
BbvI GCAGC 3 cut(s) 338, 493, 1348
BccI CCATC 8 cut(s) 92, 129, 373, 740, 776, 782, 960, 1242
BcgI CGANNNNNNTGC 4 cut(s) 337, 371, 1148, 1182
BciT130I CCWGG 3 cut(s) 537, 996, 1380
BcnI CCSGG 1 cut(s) 75
BcoDI GTCTC 2 cut(s) 512, 1095
BfaI CTAG 4 cut(s) 242, 588, 933, 945
BfmI CTRYAG 1 cut(s) 1141
BglII AGATCT 1 cut(s) 414
BisI GCNGC 5 cut(s) 324, 327, 482, 1350, 1362
BlsI GCNGC 5 cut(s) 325, 328, 483, 1351, 1363
Bme1390I CCNGG 4 cut(s) 75, 537, 996, 1380
Bme18I GGWCC 1 cut(s) 527
BmeRI GACNNNNNGTC 1 cut(s) 525
BmgT120I GGNCC 1 cut(s) 527
BmiI GGNNCC 1 cut(s) 529
BmrFI CCNGG 4 cut(s) 75, 537, 996, 1380
BmrI ACTGGG 1 cut(s) 1407
BmsI GCATC 5 cut(s) 310, 615, 827, 1179, 1326
BmuI ACTGGG 1 cut(s) 1407
BpiI GAAGAC 2 cut(s) 744, 1393
BpmI CTGGAG 2 cut(s) 351, 355
BpuEI CTTGAG 3 cut(s) 107, 233, 424
BpuMI CCSGG 1 cut(s) 75
BsaAI YACGTR 2 cut(s) 929, 1130
BsaHI GRCGYC 1 cut(s) 1201
BsaJI CCNNGG 1 cut(s) 1379
BsaXI ACNNNNNCTCC 2 cut(s) 1186, 1216
Bsc4I CCNNNNNNNGG 2 cut(s) 536, 1380
Bse1I ACTGG 1 cut(s) 1402
BseBI CCWGG 3 cut(s) 537, 996, 1380
BseDI CCNNGG 1 cut(s) 1379
BseGI GGATG 7 cut(s) 384, 427, 630, 682, 721, 971, 1253
BseLI CCNNNNNNNGG 2 cut(s) 536, 1380
BseNI ACTGG 1 cut(s) 1402
BseRI GAGGAG 3 cut(s) 686, 725, 1207
BseXI GCAGC 3 cut(s) 338, 493, 1348
Bsh1236I CGCG 1 cut(s) 1347
BsiSI CCGG 1 cut(s) 74
BslFI GGGAC 2 cut(s) 513, 595
BslI CCNNNNNNNGG 2 cut(s) 536, 1380
BsmAI GTCTC 2 cut(s) 512, 1095
BsmFI GGGAC 2 cut(s) 513, 595
BsmI GAATGC 1 cut(s) 301
Bsp143I GATC 4 cut(s) 46, 414, 998, 1109
BspACI CCGC 4 cut(s) 324, 563, 1345, 1350
BspFNI CGCG 1 cut(s) 1347
BspLI GGNNCC 1 cut(s) 529
BspPI GGATC 3 cut(s) 54, 1006, 1117
BspQI GCTCTTC 1 cut(s) 439
BsrI ACTGG 1 cut(s) 1402
BssECI CCNNGG 1 cut(s) 1379
BssMI GATC 4 cut(s) 46, 414, 998, 1109
BssNI GRCGYC 1 cut(s) 1201
Bst2UI CCWGG 3 cut(s) 537, 996, 1380
Bst4CI ACNGT 5 cut(s) 117, 497, 734, 896, 1231
Bst6I CTCTTC 6 cut(s) 439, 651, 657, 696, 759, 875
BstACI GRCGYC 1 cut(s) 1201
BstBAI YACGTR 2 cut(s) 929, 1130
BstC8I GCNNGC 1 cut(s) 301
BstDEI CTNAG 1 cut(s) 125
BstF5I GGATG 7 cut(s) 384, 427, 630, 682, 721, 971, 1253
BstFNI CGCG 1 cut(s) 1347
BstHHI GCGC 1 cut(s) 1349
BstKTI GATC 4 cut(s) 49, 417, 1001, 1112
BstMAI GTCTC 2 cut(s) 512, 1095
BstMBI GATC 4 cut(s) 46, 414, 998, 1109
BstMWI GCNNNNNNNGC 3 cut(s) 323, 945, 1358
BstNI CCWGG 3 cut(s) 537, 996, 1380
BstNSI RCATGY 1 cut(s) 362
BstSCI CCNGG 4 cut(s) 73, 535, 994, 1378
BstSFI CTRYAG 1 cut(s) 1141
BstUI CGCG 1 cut(s) 1347
BstV1I GCAGC 3 cut(s) 338, 493, 1348
BstV2I GAAGAC 2 cut(s) 744, 1393
BstX2I RGATCY 2 cut(s) 414, 1109
BstXI CCANNNNNNTGG 2 cut(s) 287, 379
BstYI RGATCY 2 cut(s) 414, 1109
BtgZI GCGATG 1 cut(s) 795
BtsCI GGATG 7 cut(s) 384, 427, 630, 682, 721, 971, 1253
BtsIMutI CAGTG 3 cut(s) 122, 630, 739
Cac8I GCNNGC 1 cut(s) 301
CaiI CAGNNNCTG 1 cut(s) 1361
CfoI GCGC 1 cut(s) 1349
Cfr13I GGNCC 1 cut(s) 527
CseI GACGC 2 cut(s) 1190, 1285
Csp6I GTAC 4 cut(s) 107, 498, 1131, 1325
CviAII CATG 6 cut(s) 359, 524, 605, 1087, 1217, 1265
CviQI GTAC 4 cut(s) 107, 498, 1131, 1325
DdeI CTNAG 1 cut(s) 125
DpnI GATC 4 cut(s) 48, 416, 1000, 1111
DpnII GATC 4 cut(s) 46, 414, 998, 1109
DriI GACNNNNNGTC 1 cut(s) 525
Eam1104I CTCTTC 6 cut(s) 439, 651, 657, 696, 759, 875
Eam1105I GACNNNNNGTC 1 cut(s) 525
EarI CTCTTC 6 cut(s) 439, 651, 657, 696, 759, 875
Eco47I GGWCC 1 cut(s) 527
Eco57I CTGAAG 1 cut(s) 860
Eco72I CACGTG 1 cut(s) 929
EcoRII CCWGG 3 cut(s) 535, 994, 1378
EcoT22I ATGCAT 1 cut(s) 360
FaeI CATG 6 cut(s) 362, 527, 608, 1090, 1220, 1268
FaqI GGGAC 2 cut(s) 513, 595
FatI CATG 6 cut(s) 358, 523, 604, 1086, 1216, 1264
Fnu4HI GCNGC 5 cut(s) 324, 327, 482, 1350, 1362
FokI GGATG 7 cut(s) 391, 434, 637, 689, 728, 978, 1260
Fsp4HI GCNGC 5 cut(s) 324, 327, 482, 1350, 1362
FspBI CTAG 4 cut(s) 242, 588, 933, 945
GlaI GCGC 1 cut(s) 1348
GluI GCNGC 5 cut(s) 324, 327, 482, 1350, 1362
GsuI CTGGAG 2 cut(s) 351, 355
HapII CCGG 1 cut(s) 74
HgaI GACGC 2 cut(s) 1190, 1285
HhaI GCGC 1 cut(s) 1349
Hin1I GRCGYC 1 cut(s) 1201
Hin1II CATG 6 cut(s) 362, 527, 608, 1090, 1220, 1268
Hin6I GCGC 1 cut(s) 1347
HinP1I GCGC 1 cut(s) 1347
HincII GTYRAC 1 cut(s) 1308
HindII GTYRAC 1 cut(s) 1308
HinfI GANTC 4 cut(s) 129, 401, 806, 1070
HpaII CCGG 1 cut(s) 74
HphI GGTGA 1 cut(s) 1298
Hpy166II GTNNAC 2 cut(s) 280, 1308
Hpy188I TCNGA 7 cut(s) 174, 268, 414, 879, 1060, 1278, 1417
Hpy188III TCNNGA 4 cut(s) 86, 372, 441, 821
Hpy8I GTNNAC 2 cut(s) 280, 1308
HpyAV CCTTC 3 cut(s) 193, 1104, 1149
HpyCH4III ACNGT 5 cut(s) 117, 497, 734, 896, 1231
HpyCH4IV ACGT 2 cut(s) 928, 1129
HpyCH4V TGCA 7 cut(s) 303, 317, 358, 628, 1120, 1364, 1371
HpyF10VI GCNNNNNNNGC 3 cut(s) 323, 945, 1358
HpyF3I CTNAG 1 cut(s) 125
HpySE526I ACGT 2 cut(s) 928, 1129
Hsp92I GRCGYC 1 cut(s) 1201
Hsp92II CATG 6 cut(s) 362, 527, 608, 1090, 1220, 1268
HspAI GCGC 1 cut(s) 1347
Kzo9I GATC 4 cut(s) 46, 414, 998, 1109
LguI GCTCTTC 1 cut(s) 439
LmnI GCTCC 2 cut(s) 374, 618
Lsp1109I GCAGC 3 cut(s) 338, 493, 1348
LweI GCATC 5 cut(s) 310, 615, 827, 1179, 1326
MaeI CTAG 4 cut(s) 242, 588, 933, 945
MaeII ACGT 2 cut(s) 928, 1129
MaeIII GTNAC 3 cut(s) 56, 1028, 1333
MalI GATC 4 cut(s) 48, 416, 1000, 1111
MboI GATC 4 cut(s) 46, 414, 998, 1109
MflI RGATCY 2 cut(s) 414, 1109
MlyI GAGTC 1 cut(s) 395
MmeI TCCRAC 2 cut(s) 176, 1395
Mph1103I ATGCAT 1 cut(s) 360
MseI TTAA 4 cut(s) 224, 983, 1185, 1260
MslI CAYNNNNRTG 3 cut(s) 96, 285, 633
MspA1I CMGCKG 2 cut(s) 329, 563
MspI CCGG 1 cut(s) 74
MspR9I CCNGG 4 cut(s) 75, 537, 996, 1380
Mva1269I GAATGC 1 cut(s) 301
MvaI CCWGG 3 cut(s) 537, 996, 1380
MvnI CGCG 1 cut(s) 1347
MwoI GCNNNNNNNGC 3 cut(s) 323, 945, 1358
NciI CCSGG 1 cut(s) 75
NdeII GATC 4 cut(s) 46, 414, 998, 1109
NlaIII CATG 6 cut(s) 362, 527, 608, 1090, 1220, 1268
NlaIV GGNNCC 1 cut(s) 529
NmuCI GTSAC 1 cut(s) 1028
NsiI ATGCAT 1 cut(s) 360
NspI RCATGY 1 cut(s) 362
PciSI GCTCTTC 1 cut(s) 439
PctI GAATGC 1 cut(s) 301
PfeI GAWTC 3 cut(s) 129, 806, 1070
PfoI TCCNGGA 1 cut(s) 73
PkrI GCNGC 5 cut(s) 325, 328, 483, 1351, 1363
PleI GAGTC 1 cut(s) 395
PmaCI CACGTG 1 cut(s) 929
PmlI CACGTG 1 cut(s) 929
PpsI GAGTC 1 cut(s) 395
Ppu21I YACGTR 2 cut(s) 929, 1130
Psp6I CCWGG 3 cut(s) 535, 994, 1378
PspCI CACGTG 1 cut(s) 929
PspGI CCWGG 3 cut(s) 535, 994, 1378
PspN4I GGNNCC 1 cut(s) 529
PspPI GGNCC 1 cut(s) 527
PstNI CAGNNNCTG 1 cut(s) 1361
PsuI RGATCY 2 cut(s) 414, 1109
PvuII CAGCTG 1 cut(s) 329
RsaI GTAC 4 cut(s) 108, 499, 1132, 1326
RsaNI GTAC 4 cut(s) 107, 498, 1131, 1325
RseI CAYNNNNRTG 3 cut(s) 96, 285, 633
SapI GCTCTTC 1 cut(s) 439
SaqAI TTAA 4 cut(s) 224, 983, 1185, 1260
SatI GCNGC 5 cut(s) 324, 327, 482, 1350, 1362
Sau3AI GATC 4 cut(s) 46, 414, 998, 1109
Sau96I GGNCC 1 cut(s) 527
SchI GAGTC 1 cut(s) 395
ScrFI CCNGG 4 cut(s) 75, 537, 996, 1380
SfaNI GCATC 5 cut(s) 310, 615, 827, 1179, 1326
SfcI CTRYAG 1 cut(s) 1141
SinI GGWCC 1 cut(s) 527
SmiMI CAYNNNNRTG 3 cut(s) 96, 285, 633
SmlI CTYRAG 3 cut(s) 86, 212, 439
SmoI CTYRAG 3 cut(s) 86, 212, 439
SsiI CCGC 4 cut(s) 324, 563, 1345, 1350
SspMI CTAG 4 cut(s) 242, 588, 933, 945
StyD4I CCNGG 4 cut(s) 73, 535, 994, 1378
TaaI ACNGT 5 cut(s) 117, 497, 734, 896, 1231
TaiI ACGT 2 cut(s) 931, 1132
TaqI TCGA 2 cut(s) 593, 1001
TatI WGTACW 2 cut(s) 497, 1324
TauI GCSGC 2 cut(s) 326, 1352
TfiI GAWTC 3 cut(s) 129, 806, 1070
Tru1I TTAA 4 cut(s) 224, 983, 1185, 1260
Tru9I TTAA 4 cut(s) 224, 983, 1185, 1260
TscAI CASTG 3 cut(s) 122, 637, 739
TseFI GTSAC 1 cut(s) 1028
TseI GCWGC 3 cut(s) 326, 481, 1361
Tsp45I GTSAC 1 cut(s) 1028
TspDTI ATGAA 7 cut(s) 453, 714, 732, 738, 768, 774, 1036
TspRI CASTG 3 cut(s) 122, 637, 739
VpaK11BI GGWCC 1 cut(s) 527
XapI RAATTY 5 cut(s) 69, 203, 262, 816, 1256
XceI RCATGY 1 cut(s) 362
XspI CTAG 4 cut(s) 242, 588, 933, 945
Zsp2I ATGCAT 1 cut(s) 360
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.