Prupe.7G144200_v2.0.a1

B3 DNA binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp07
OG0000593
Physical Location & Seq
Forward (+)
15807921 .. 15810343
2423 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.7G144200.1

Sequence Viewer

Length: 939 bp
ATGGCTAGAAGAAGAAGAAAACCCTCCTTTTTCAAGGCTCTTGTACATGACTTCTCCAGCCAGTTGGAAATCCCTCCGGCTTTTTACGTGCATTTTGCTGGAGAGGTACCTCGGAGGTCTCTTCTTCGGACCCCAGCTGGAACTTGGAATGTAAATGTGGAAAAGGTTAATGATGGTTTCTTTTTCCAAAAGGGTTGGAAGGAATTTGTGCACGACCATGGTTTAAAGCTTTGTGAATTTCTAATTTTTCGATATGCTGGAAATTCGAAATTTGATGTTGACGTATATGGAAGAAATGGGTGCGCAAAGGAGTTTGTAATGGCGGTTAGCAAAAATGACAGATCCCTTGAGGAGGAACATGATAATACAATTCATGGAACGCAGAATGTAGATGGCCAGGGCTTTGGCTCAGCTGATGGAACTACAACTAGTCAAGCCACTTGTGCCATAAAAAATGAAGTAATTGATGTTGATTCAGATACATCGATGGATGTTGATGAGCCTGATACTCAAGAGGATCAACTTAACGAGGATTCCGAAATTGGGTTGCCTAGAGAGTTGGGTTCTACAGAGCATTTTTATTCAAGGACTCAAATGGAAAATCCGAGTGAAGCTCTTGAAGCAGCCAAAAAATTCTCTTCTAAGCATCCTTTCTTCAGAGCTATTATGCGTCAAGCATATGTGGACAAGGGAAGACTGGTTATACCAAAGAGCTTTCAGACTAGTTGTATTGGAAAAAAAAAGTATGTCAGGCTTCAAGACTCAATGAGGAGGTGGATTGTGAAGTGTATTTCTCGCCGCAACTCTAGCAGGCTCTCTGCAGGGTGGTACCAGTTTGTAAGGGATCGTGCTTTGAAAGTGGGAGATGTTTGTGTGTTTGAGCTGATTGACAGAAATGATGCTGTGATGAAAGTTTCTATTTTCAGATGCAACAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

313

Amino Acids

35.7

Weight (kDa)

8.39

Isoelectric Point (pI)

38.87

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 304
Acc65I GGTACC 2 cut(s) 106, 828
AccB1I GGYRCC 2 cut(s) 106, 828
AciI CCGC 2 cut(s) 323, 799
AclWI GGATC 3 cut(s) 336, 525, 852
AcoI YGGCCR 1 cut(s) 394
AcsI RAATTY 5 cut(s) 203, 236, 262, 269, 632
AcuI CTGAAG 1 cut(s) 640
AfaI GTAC 3 cut(s) 45, 108, 830
AfiI CCNNNNNNNGG 2 cut(s) 352, 543
AgsI TTSAA 5 cut(s) 34, 585, 620, 758, 856
AhlI ACTAGT 2 cut(s) 428, 722
AjnI CCWGG 1 cut(s) 396
AluBI AGCT 7 cut(s) 137, 229, 413, 614, 662, 714, 883
AluI AGCT 7 cut(s) 137, 229, 413, 614, 662, 714, 883
Alw21I GWGCWC 1 cut(s) 213
Alw26I GTCTC 1 cut(s) 123
Alw44I GTGCAC 1 cut(s) 209
AlwI GGATC 3 cut(s) 336, 525, 852
AoxI GGCC 1 cut(s) 394
ApaLI GTGCAC 1 cut(s) 209
ApeKI GCWGC 1 cut(s) 623
ApoI RAATTY 5 cut(s) 203, 236, 262, 269, 632
Asp718I GGTACC 2 cut(s) 106, 828
AspLEI GCGC 1 cut(s) 305
AspS9I GGNCC 1 cut(s) 129
AsuII TTCGAA 1 cut(s) 266
AvaII GGWCC 1 cut(s) 129
BaeGI GKGCMC 1 cut(s) 213
BalI TGGCCA 1 cut(s) 396
BanI GGYRCC 2 cut(s) 106, 828
BbsI GAAGAC 1 cut(s) 700
Bbv12I GWGCWC 1 cut(s) 213
BbvI GCAGC 1 cut(s) 635
BccI CCATC 4 cut(s) 167, 386, 410, 481
BciT130I CCWGG 1 cut(s) 398
BcoDI GTCTC 1 cut(s) 123
BcuI ACTAGT 2 cut(s) 428, 722
BfaI CTAG 5 cut(s) 6, 429, 552, 723, 807
BfmI CTRYAG 2 cut(s) 567, 819
BisI GCNGC 2 cut(s) 624, 799
BlpI GCTNAGC 1 cut(s) 409
BlsI GCNGC 2 cut(s) 625, 800
Bme1390I CCNGG 1 cut(s) 398
Bme18I GGWCC 1 cut(s) 129
BmgT120I GGNCC 1 cut(s) 129
BmiI GGNNCC 3 cut(s) 108, 131, 830
BmrFI CCNGG 1 cut(s) 398
BmsI GCATC 3 cut(s) 655, 889, 917
BpiI GAAGAC 1 cut(s) 700
BplI GAGNNNNNCTC 2 cut(s) 598, 630
BpmI CTGGAG 2 cut(s) 40, 120
Bpu1102I GCTNAGC 1 cut(s) 409
Bpu14I TTCGAA 1 cut(s) 266
BpuEI CTTGAG 2 cut(s) 368, 495
Bsa29I ATCGAT 1 cut(s) 485
BsaAI YACGTR 1 cut(s) 88
BsaI GGTCTC 1 cut(s) 123
BsaJI CCNNGG 3 cut(s) 110, 217, 397
Bsc4I CCNNNNNNNGG 2 cut(s) 352, 543
Bse1I ACTGG 3 cut(s) 61, 702, 832
BseBI CCWGG 1 cut(s) 398
BseCI ATCGAT 1 cut(s) 485
BseDI CCNNGG 3 cut(s) 110, 217, 397
BseGI GGATG 2 cut(s) 496, 646
BseLI CCNNNNNNNGG 2 cut(s) 352, 543
BseMII CTCAG 1 cut(s) 423
BseNI ACTGG 3 cut(s) 61, 702, 832
BseRI GAGGAG 2 cut(s) 365, 784
BseSI GKGCMC 1 cut(s) 213
BseXI GCAGC 1 cut(s) 635
BseYI CCCAGC 1 cut(s) 133
BshFI GGCC 1 cut(s) 396
BshNI GGYRCC 2 cut(s) 106, 828
BshVI ATCGAT 1 cut(s) 485
BsiHKAI GWGCWC 1 cut(s) 213
BsiSI CCGG 1 cut(s) 77
BslI CCNNNNNNNGG 2 cut(s) 352, 543
BsmAI GTCTC 1 cut(s) 123
BsnI GGCC 1 cut(s) 396
Bso31I GGTCTC 1 cut(s) 123
Bsp119I TTCGAA 1 cut(s) 266
Bsp1286I GDGCHC 1 cut(s) 213
Bsp1407I TGTACA 1 cut(s) 43
Bsp143I GATC 3 cut(s) 341, 517, 844
Bsp1720I GCTNAGC 1 cut(s) 409
Bsp19I CCATGG 1 cut(s) 217
BspACI CCGC 2 cut(s) 323, 799
BspANI GGCC 1 cut(s) 396
BspCNI CTCAG 1 cut(s) 422
BspDI ATCGAT 1 cut(s) 485
BspLI GGNNCC 3 cut(s) 108, 131, 830
BspMAI CTGCAG 1 cut(s) 823
BspPI GGATC 3 cut(s) 336, 525, 852
BspT104I TTCGAA 1 cut(s) 266
BspT107I GGYRCC 2 cut(s) 106, 828
BspTNI GGTCTC 1 cut(s) 123
BsrGI TGTACA 1 cut(s) 43
BsrI ACTGG 3 cut(s) 61, 702, 832
BssECI CCNNGG 3 cut(s) 110, 217, 397
BssMI GATC 3 cut(s) 341, 517, 844
BssT1I CCWWGG 1 cut(s) 217
Bst2UI CCWGG 1 cut(s) 398
Bst4CI ACNGT 1 cut(s) 935
Bst6I CTCTTC 2 cut(s) 126, 643
BstAUI TGTACA 1 cut(s) 43
BstBAI YACGTR 1 cut(s) 88
BstBI TTCGAA 1 cut(s) 266
BstC8I GCNNGC 1 cut(s) 812
BstDEI CTNAG 2 cut(s) 409, 642
BstDSI CCRYGG 1 cut(s) 217
BstENI CCTNNNNNAGG 1 cut(s) 350
BstF5I GGATG 2 cut(s) 496, 646
BstHHI GCGC 1 cut(s) 305
BstKTI GATC 3 cut(s) 344, 520, 847
BstMAI GTCTC 1 cut(s) 123
BstMBI GATC 3 cut(s) 341, 517, 844
BstMWI GCNNNNNNNGC 3 cut(s) 443, 620, 807
BstNI CCWGG 1 cut(s) 398
BstSCI CCNGG 1 cut(s) 396
BstSFI CTRYAG 2 cut(s) 567, 819
BstSLI GKGCMC 1 cut(s) 213
BstV1I GCAGC 1 cut(s) 635
BstV2I GAAGAC 1 cut(s) 700
BstX2I RGATCY 1 cut(s) 341
BstXI CCANNNNNNTGG 2 cut(s) 64, 404
BstYI RGATCY 1 cut(s) 341
Bsu15I ATCGAT 1 cut(s) 485
BsuRI GGCC 1 cut(s) 396
BsuTUI ATCGAT 1 cut(s) 485
BtgI CCRYGG 1 cut(s) 217
BtsCI GGATG 2 cut(s) 496, 646
Cac8I GCNNGC 1 cut(s) 812
CfoI GCGC 1 cut(s) 305
Cfr13I GGNCC 1 cut(s) 129
ClaI ATCGAT 1 cut(s) 485
CseI GACGC 1 cut(s) 659
Csp6I GTAC 3 cut(s) 44, 107, 829
CviAII CATG 4 cut(s) 47, 218, 359, 374
CviQI GTAC 3 cut(s) 44, 107, 829
DdeI CTNAG 2 cut(s) 409, 642
DpnI GATC 3 cut(s) 343, 519, 846
DpnII GATC 3 cut(s) 341, 517, 844
DraI TTTAAA 1 cut(s) 225
EaeI YGGCCR 1 cut(s) 394
Eam1104I CTCTTC 2 cut(s) 126, 643
EarI CTCTTC 2 cut(s) 126, 643
Eco130I CCWWGG 1 cut(s) 217
Eco31I GGTCTC 1 cut(s) 123
Eco47I GGWCC 1 cut(s) 129
Eco57I CTGAAG 1 cut(s) 640
EcoNI CCTNNNNNAGG 1 cut(s) 350
EcoRII CCWGG 1 cut(s) 396
EcoT14I CCWWGG 1 cut(s) 217
ErhI CCWWGG 1 cut(s) 217
FaeI CATG 4 cut(s) 50, 221, 362, 377
FatI CATG 4 cut(s) 46, 217, 358, 373
FauNDI CATATG 1 cut(s) 679
Fnu4HI GCNGC 2 cut(s) 624, 799
FokI GGATG 2 cut(s) 503, 633
Fsp4HI GCNGC 2 cut(s) 624, 799
FspBI CTAG 5 cut(s) 6, 429, 552, 723, 807
FspI TGCGCA 1 cut(s) 304
GlaI GCGC 1 cut(s) 304
GluI GCNGC 2 cut(s) 624, 799
GsaI CCCAGC 1 cut(s) 137
GsuI CTGGAG 2 cut(s) 40, 120
HaeIII GGCC 1 cut(s) 396
HapII CCGG 1 cut(s) 77
HgaI GACGC 1 cut(s) 659
HhaI GCGC 1 cut(s) 305
Hin1II CATG 4 cut(s) 50, 221, 362, 377
Hin6I GCGC 1 cut(s) 303
HinP1I GCGC 1 cut(s) 303
HincII GTYRAC 1 cut(s) 280
HindII GTYRAC 1 cut(s) 280
HindIII AAGCTT 1 cut(s) 227
HinfI GANTC 4 cut(s) 473, 533, 589, 761
HpaII CCGG 1 cut(s) 77
Hpy166II GTNNAC 3 cut(s) 211, 280, 685
Hpy188I TCNGA 8 cut(s) 114, 129, 478, 538, 606, 659, 720, 926
Hpy188III TCNNGA 3 cut(s) 512, 617, 758
Hpy8I GTNNAC 3 cut(s) 211, 280, 685
HpyAV CCTTC 1 cut(s) 193
HpyCH4III ACNGT 1 cut(s) 935
HpyCH4IV ACGT 2 cut(s) 87, 282
HpyCH4V TGCA 4 cut(s) 91, 211, 821, 930
HpyF10VI GCNNNNNNNGC 3 cut(s) 443, 620, 807
HpyF3I CTNAG 2 cut(s) 409, 642
HpySE526I ACGT 2 cut(s) 87, 282
Hsp92II CATG 4 cut(s) 50, 221, 362, 377
HspAI GCGC 1 cut(s) 303
KpnI GGTACC 2 cut(s) 110, 832
Kzo9I GATC 3 cut(s) 341, 517, 844
Lsp1109I GCAGC 1 cut(s) 635
LweI GCATC 3 cut(s) 655, 889, 917
MaeI CTAG 5 cut(s) 6, 429, 552, 723, 807
MaeII ACGT 2 cut(s) 87, 282
MalI GATC 3 cut(s) 343, 519, 846
MboI GATC 3 cut(s) 341, 517, 844
MboII GAAGA 9 cut(s) 21, 24, 27, 113, 116, 303, 630, 646, 705
MflI RGATCY 1 cut(s) 341
MhlI GDGCHC 1 cut(s) 213
MlsI TGGCCA 1 cut(s) 396
MluCI AATT 9 cut(s) 203, 236, 243, 262, 269, 369, 462, 540, 632
MluNI TGGCCA 1 cut(s) 396
MlyI GAGTC 2 cut(s) 583, 755
MmeI TCCRAC 2 cut(s) 45, 176
Mox20I TGGCCA 1 cut(s) 396
MscI TGGCCA 1 cut(s) 396
MseI TTAA 3 cut(s) 168, 224, 525
MslI CAYNNNNRTG 1 cut(s) 216
Msp20I TGGCCA 1 cut(s) 396
MspA1I CMGCKG 2 cut(s) 137, 413
MspI CCGG 1 cut(s) 77
MspR9I CCNGG 1 cut(s) 398
MvaI CCWGG 1 cut(s) 398
MwoI GCNNNNNNNGC 3 cut(s) 443, 620, 807
NcoI CCATGG 1 cut(s) 217
NdeI CATATG 1 cut(s) 679
NdeII GATC 3 cut(s) 341, 517, 844
NlaIII CATG 4 cut(s) 50, 221, 362, 377
NlaIV GGNNCC 3 cut(s) 108, 131, 830
NsbI TGCGCA 1 cut(s) 304
NspV TTCGAA 1 cut(s) 266
PcsI WCGNNNNNNNCGW 1 cut(s) 534
PfeI GAWTC 2 cut(s) 473, 533
PkrI GCNGC 2 cut(s) 625, 800
PleI GAGTC 2 cut(s) 583, 755
PpsI GAGTC 2 cut(s) 583, 755
Ppu21I YACGTR 1 cut(s) 88
Psp6I CCWGG 1 cut(s) 396
PspFI CCCAGC 1 cut(s) 133
PspGI CCWGG 1 cut(s) 396
PspN4I GGNNCC 3 cut(s) 108, 131, 830
PspPI GGNCC 1 cut(s) 129
PstI CTGCAG 1 cut(s) 823
PsuI RGATCY 1 cut(s) 341
PvuII CAGCTG 2 cut(s) 137, 413
RsaI GTAC 3 cut(s) 45, 108, 830
RsaNI GTAC 3 cut(s) 44, 107, 829
RseI CAYNNNNRTG 1 cut(s) 216
SaqAI TTAA 3 cut(s) 168, 224, 525
SatI GCNGC 2 cut(s) 624, 799
Sau3AI GATC 3 cut(s) 341, 517, 844
Sau96I GGNCC 1 cut(s) 129
SchI GAGTC 2 cut(s) 583, 755
ScrFI CCNGG 1 cut(s) 398
SduI GDGCHC 1 cut(s) 213
SfaNI GCATC 3 cut(s) 655, 889, 917
SfcI CTRYAG 2 cut(s) 567, 819
SfuI TTCGAA 1 cut(s) 266
SinI GGWCC 1 cut(s) 129
SmiMI CAYNNNNRTG 1 cut(s) 216
SmlI CTYRAG 2 cut(s) 347, 510
SmoI CTYRAG 2 cut(s) 347, 510
SpeI ACTAGT 2 cut(s) 428, 722
Sse9I AATT 9 cut(s) 203, 236, 243, 262, 269, 369, 462, 540, 632
SsiI CCGC 2 cut(s) 323, 799
SspMI CTAG 5 cut(s) 6, 429, 552, 723, 807
StyD4I CCNGG 1 cut(s) 396
StyI CCWWGG 1 cut(s) 217
TaaI ACNGT 1 cut(s) 935
TaiI ACGT 2 cut(s) 90, 285
TaqI TCGA 3 cut(s) 250, 266, 485
TasI AATT 9 cut(s) 203, 236, 243, 262, 269, 369, 462, 540, 632
TatI WGTACW 1 cut(s) 43
TauI GCSGC 1 cut(s) 801
TfiI GAWTC 2 cut(s) 473, 533
Tru1I TTAA 3 cut(s) 168, 224, 525
Tru9I TTAA 3 cut(s) 168, 224, 525
TseI GCWGC 1 cut(s) 623
TspDTI ATGAA 3 cut(s) 362, 471, 923
VneI GTGCAC 1 cut(s) 209
VpaK11BI GGWCC 1 cut(s) 129
XagI CCTNNNNNAGG 1 cut(s) 350
XapI RAATTY 5 cut(s) 203, 236, 262, 269, 632
XcmI CCANNNNNNNNNTGG 1 cut(s) 141
XspI CTAG 5 cut(s) 6, 429, 552, 723, 807
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.