RLG00000018437

B3 DNA binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0000593
Physical Location & Seq
Forward (+)
29755086 .. 29756910
1825 bp
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UTR
Exon/CDS
Intron
RLM00000018437

Sequence Viewer

Length: 924 bp
ATGGCAAGAAAAAGAAAGCCTTCCTTTTTCAAGGTTCTTATAGGTGACTTCTCTGACAAGCTGAGAATCCCACAGGCTTTTCGTAAGCATTTGGCTGCAAGTGTACTTCAACAATCCCATCTTCGGACCCCTGCTGGAACTTGGCTTGTTGAAGTGAAAAGCGATAACGGCAGGTTCTTTTTACGAAAGGGTTGGAAGCAATTTGTGCATGATAATGGTTTAAAGCTTGGAGAATTTCTAATTTTTCGATATGCTGGAAATTCAAAATTCTATGTTGACATATATGGAAGACATGGTTGCAAAAAGGAGTTTGTAATGCCGGCTACGAGAAGTGAAAAACCATTTAAAAAGAGGCACGGTAATGAAAGTCATAGAACAAACGCTGTTATGAGTTCCAGCATACATCCAAAGAAGAAGATGGATTATCAGAAGTTGAAAGGTTCAGCTGGTGAAAATAAAACTAGTCAAGAAACTATTGCCGTCAAATCTGAACCAATTGATTTTGAATTGGAAACATCTATGGATATTGACGAGTCAATTAGAAATATGAATTCTAGAGAGACTTTGTCTTTCCAGCCTCAAGTGGCAAATAGTGCTCTAGAAGCAGCCAACAGATACTCATCGAACCATCCTTGCTTCAGAACTCTTTTGACCGAAACTTATGTGACCAAAGGAATTCTGCATATACCATGTACCTTTGTGAAAGATTATATTAAGATAAAGAAAAGGACAGCTGAGCTTCAAGTTTCAAACAGGTTGTGGTCTGTGAAGTGGATTAATGACTCTCGCTCAAGTAGATTCTCTCAAGGTTGGAATGAGTTTGTAAGGCAGAATGATCTGAAAGTGGGAGATGTTTGTGTCTTTGAGCTGATTAAGCAGACGAATGATGTTGTGATGATGAAAGTTTCTTTTTTCAGATGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

308

Amino Acids

35.56

Weight (kDa)

9.88

Isoelectric Point (pI)

38.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 9 - 96 2.4e-14 B3 DNA binding domain
B3 PF02362 213 - 296 1.5e-15 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 162
AcsI RAATTY 5 cut(s) 233, 259, 266, 550, 675
AcuI CTGAAG 1 cut(s) 622
AfaI GTAC 2 cut(s) 105, 694
AfiI CCNNNNNNNGG 1 cut(s) 123
AgsI TTSAA 8 cut(s) 31, 110, 152, 264, 436, 506, 743, 750
AhlI ACTAGT 1 cut(s) 461
AluBI AGCT 6 cut(s) 61, 226, 446, 734, 739, 868
AluI AGCT 6 cut(s) 61, 226, 446, 734, 739, 868
Alw21I GWGCWC 1 cut(s) 598
Alw26I GTCTC 1 cut(s) 554
ApeKI GCWGC 2 cut(s) 95, 605
ApoI RAATTY 5 cut(s) 233, 259, 266, 550, 675
AseI ATTAAT 1 cut(s) 777
Asp700I GAANNNNTTC 1 cut(s) 19
AspS9I GGNCC 1 cut(s) 126
AsuHPI GGTGA 2 cut(s) 56, 461
AvaII GGWCC 1 cut(s) 126
BbsI GAAGAC 1 cut(s) 295
Bbv12I GWGCWC 1 cut(s) 598
BbvI GCAGC 2 cut(s) 82, 617
BccI CCATC 3 cut(s) 126, 412, 636
BceAI ACGGC 2 cut(s) 184, 464
BcoDI GTCTC 1 cut(s) 554
BcuI ACTAGT 1 cut(s) 461
BfaI CTAG 3 cut(s) 462, 555, 599
BfuAI ACCTGC 1 cut(s) 162
BisI GCNGC 2 cut(s) 96, 606
BlpI GCTNAGC 1 cut(s) 735
BlsI GCNGC 2 cut(s) 97, 607
Bme18I GGWCC 1 cut(s) 126
BmgT120I GGNCC 1 cut(s) 126
BmiI GGNNCC 1 cut(s) 128
BpiI GAAGAC 1 cut(s) 295
Bpu1102I GCTNAGC 1 cut(s) 735
BpuEI CTTGAG 3 cut(s) 564, 775, 789
BsaBI GATNNNNATC 1 cut(s) 619
Bsc4I CCNNNNNNNGG 1 cut(s) 123
Bse118I RCCGGY 1 cut(s) 319
Bse8I GATNNNNATC 1 cut(s) 619
BseGI GGATG 2 cut(s) 403, 628
BseJI GATNNNNATC 1 cut(s) 619
BseLI CCNNNNNNNGG 1 cut(s) 123
BseMII CTCAG 2 cut(s) 53, 726
BseXI GCAGC 2 cut(s) 82, 617
BsiHKAI GWGCWC 1 cut(s) 598
BsiSI CCGG 1 cut(s) 320
BslI CCNNNNNNNGG 1 cut(s) 123
BsmAI GTCTC 1 cut(s) 554
Bsp1286I GDGCHC 1 cut(s) 598
Bsp143I GATC 1 cut(s) 835
Bsp1720I GCTNAGC 1 cut(s) 735
BspCNI CTCAG 2 cut(s) 54, 727
BspLI GGNNCC 1 cut(s) 128
BspMI ACCTGC 1 cut(s) 162
BsrFI RCCGGY 1 cut(s) 319
BssAI RCCGGY 1 cut(s) 319
BssMI GATC 1 cut(s) 835
Bst4CI ACNGT 1 cut(s) 359
BstAPI GCANNNNNTGC 2 cut(s) 205, 593
BstC8I GCNNGC 1 cut(s) 321
BstDEI CTNAG 2 cut(s) 62, 735
BstF5I GGATG 2 cut(s) 403, 628
BstKTI GATC 1 cut(s) 838
BstMAI GTCTC 1 cut(s) 554
BstMBI GATC 1 cut(s) 835
BstMWI GCNNNNNNNGC 5 cut(s) 168, 205, 593, 602, 874
BstV1I GCAGC 2 cut(s) 82, 617
BstV2I GAAGAC 1 cut(s) 295
BtsCI GGATG 2 cut(s) 403, 628
BveI ACCTGC 1 cut(s) 162
Cac8I GCNNGC 1 cut(s) 321
Cfr10I RCCGGY 1 cut(s) 319
Cfr13I GGNCC 1 cut(s) 126
Csp6I GTAC 2 cut(s) 104, 693
CviAII CATG 3 cut(s) 209, 293, 690
CviQI GTAC 2 cut(s) 104, 693
DdeI CTNAG 2 cut(s) 62, 735
DpnI GATC 1 cut(s) 837
DpnII GATC 1 cut(s) 835
DraI TTTAAA 2 cut(s) 222, 346
Eco47I GGWCC 1 cut(s) 126
Eco57I CTGAAG 1 cut(s) 622
EcoRI GAATTC 2 cut(s) 550, 675
FaeI CATG 3 cut(s) 212, 296, 693
FalI AAGNNNNNCTT 3 cut(s) 36, 8, 40
FatI CATG 3 cut(s) 208, 292, 689
Fnu4HI GCNGC 2 cut(s) 96, 606
FokI GGATG 2 cut(s) 390, 615
Fsp4HI GCNGC 2 cut(s) 96, 606
FspBI CTAG 3 cut(s) 462, 555, 599
GluI GCNGC 2 cut(s) 96, 606
HapII CCGG 1 cut(s) 320
Hin1II CATG 3 cut(s) 212, 296, 693
HincII GTYRAC 1 cut(s) 277
HindII GTYRAC 1 cut(s) 277
HindIII AAGCTT 1 cut(s) 224
HinfI GANTC 4 cut(s) 66, 533, 782, 798
HpaII CCGG 1 cut(s) 320
HphI GGTGA 2 cut(s) 56, 461
Hpy166II GTNNAC 2 cut(s) 104, 277
Hpy188I TCNGA 7 cut(s) 55, 126, 429, 490, 641, 840, 917
Hpy188III TCNNGA 3 cut(s) 467, 555, 599
Hpy8I GTNNAC 2 cut(s) 104, 277
HpyAV CCTTC 1 cut(s) 30
HpyCH4III ACNGT 1 cut(s) 359
HpyCH4V TGCA 4 cut(s) 98, 208, 300, 682
HpyF10VI GCNNNNNNNGC 5 cut(s) 168, 205, 593, 602, 874
HpyF3I CTNAG 2 cut(s) 62, 735
Hsp92II CATG 3 cut(s) 212, 296, 693
KroI GCCGGC 1 cut(s) 319
KroNI GCCGGC 1 cut(s) 321
Kzo9I GATC 1 cut(s) 835
Lsp1109I GCAGC 2 cut(s) 82, 617
MaeI CTAG 3 cut(s) 462, 555, 599
MaeIII GTNAC 2 cut(s) 44, 664
MalI GATC 1 cut(s) 837
MboI GATC 1 cut(s) 835
MboII GAAGA 4 cut(s) 113, 300, 424, 427
MfeI CAATTG 1 cut(s) 495
MhlI GDGCHC 1 cut(s) 598
MlyI GAGTC 2 cut(s) 542, 776
MmeI TCCRAC 2 cut(s) 173, 791
MnlI CCTC 2 cut(s) 345, 588
MroNI GCCGGC 1 cut(s) 319
MroXI GAANNNNTTC 1 cut(s) 19
MseI TTAA 5 cut(s) 221, 345, 714, 777, 873
MslI CAYNNNNRTG 2 cut(s) 213, 360
MspA1I CMGCKG 2 cut(s) 446, 734
MspI CCGG 1 cut(s) 320
MunI CAATTG 1 cut(s) 495
MwoI GCNNNNNNNGC 5 cut(s) 168, 205, 593, 602, 874
NaeI GCCGGC 1 cut(s) 321
NdeII GATC 1 cut(s) 835
NgoMIV GCCGGC 1 cut(s) 319
NlaIII CATG 3 cut(s) 212, 296, 693
NlaIV GGNNCC 1 cut(s) 128
NmuCI GTSAC 2 cut(s) 44, 664
PdiI GCCGGC 1 cut(s) 321
PdmI GAANNNNTTC 1 cut(s) 19
PfeI GAWTC 2 cut(s) 66, 798
PflFI GACNNNGTC 1 cut(s) 565
PkrI GCNGC 2 cut(s) 97, 607
PleI GAGTC 2 cut(s) 541, 776
PpsI GAGTC 2 cut(s) 541, 776
PshBI ATTAAT 1 cut(s) 777
PspN4I GGNNCC 1 cut(s) 128
PspPI GGNCC 1 cut(s) 126
PsyI GACNNNGTC 1 cut(s) 565
PvuII CAGCTG 2 cut(s) 446, 734
RsaI GTAC 2 cut(s) 105, 694
RsaNI GTAC 2 cut(s) 104, 693
RseI CAYNNNNRTG 2 cut(s) 213, 360
SaqAI TTAA 5 cut(s) 221, 345, 714, 777, 873
SatI GCNGC 2 cut(s) 96, 606
Sau3AI GATC 1 cut(s) 835
Sau96I GGNCC 1 cut(s) 126
SchI GAGTC 2 cut(s) 542, 776
SduI GDGCHC 1 cut(s) 598
SinI GGWCC 1 cut(s) 126
SmiMI CAYNNNNRTG 2 cut(s) 213, 360
SmlI CTYRAG 3 cut(s) 579, 790, 804
SmoI CTYRAG 3 cut(s) 579, 790, 804
SpeI ACTAGT 1 cut(s) 461
SspMI CTAG 3 cut(s) 462, 555, 599
TaaI ACNGT 1 cut(s) 359
TaqI TCGA 2 cut(s) 247, 623
TaqII GACCGA 1 cut(s) 668
TatI WGTACW 1 cut(s) 103
TfiI GAWTC 2 cut(s) 66, 798
Tru1I TTAA 5 cut(s) 221, 345, 714, 777, 873
Tru9I TTAA 5 cut(s) 221, 345, 714, 777, 873
TseFI GTSAC 2 cut(s) 44, 664
TseI GCWGC 2 cut(s) 95, 605
Tsp45I GTSAC 2 cut(s) 44, 664
TspDTI ATGAA 3 cut(s) 378, 563, 914
Tth111I GACNNNGTC 1 cut(s) 565
VpaK11BI GGWCC 1 cut(s) 126
VspI ATTAAT 1 cut(s) 777
XapI RAATTY 5 cut(s) 233, 259, 266, 550, 675
XbaI TCTAGA 2 cut(s) 554, 598
XmnI GAANNNNTTC 1 cut(s) 19
XspI CTAG 3 cut(s) 462, 555, 599
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.