Rh2DG303000

B3 DNA binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0000593
Physical Location & Seq
Forward (+)
36226671 .. 36234029
7359 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG303000.1

Sequence Viewer

Length: 1065 bp
ATGACCTCATCCAGCAAGAGCTGTAGCGATATCCTAGAAATTTTGACACAAAATACGAGTGCGAGACACTGGGTATGGGATAGGGATTCCGAACCCTTGCTCCGTCGCGAAGACAAAGAAGTAGACGAACACAAAATCGTCATGGCAAGAAAAAGAAAGCCTTCCTTTTTCAAGGTTCTTATAGGTGACTTCTCTGACAAGCTGAGAATCCCACAGGCTTTTCGTAAGCATTTGGCTGCAAGTGTACTTCAACAATCCCATCTTCGGACCCCTGCTGGAACTTGGCTTGTCGATGTGAAAAGCGATAACGGCAGGTTCTTTTTACAAAAGGGTTGGAAGCAATTTGTGCATGATAATGGTTTAAAGCTCGGAGAGTTTCTAATTTTCCGATATGCTGGAAATTCAAAATTCTATGTTGACATATATGGAAGACATGGTTGCAAAAAGGAGTTTATAATGCCGGCTAGGAGTAGTGAAAAACCATTTAAAAAGAGGCACGGTAATGAAAGTCATAGAACAAACGCTGTTATGAGTTCCAGCATACATCCAAAGAAGAAGATGGATTATCAGAAGTCAAAAGGTTCAGCTGGTGAAAATAAAACTAGTCAAGAAACTATTTCCGTCAAATCTGAACCAATTGATTTTGAATTGGAAACATGTATGGATATTGACGAGTCAATTAGAAATATGAATTCTAGAGAGACTTTGTCTTTCCAACCTCAAGTGGCAAATAGTGGTCTAGAAGCAGCCAACAGATACTCATCGAACCATCCTTGCTTCAGAACTCTTTTGACCGAAACTTATGTGACCAAAGGAATTCTGCATATACCATGTACCTTTGTGAAAGATTATATTAAGATAAAGAAAAGGACAGCTGAGCTTCAAGTTTCAAACAGGTTGTGGTCTGTGAAGTGGATTAATGACTCTCGCTCAAGTAGATTCTCTCAAGGTTGGAATGAGTTTGTAAGGCAGAATGATCTGAAAGTGGGAGATGTTTGTGTCTTTGAGCTGATTAAGCAGACGAATGATGTTGTGATGATGAAAGTTTCTTTTTTCAGACGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

354

Amino Acids

41.09

Weight (kDa)

9.66

Isoelectric Point (pI)

45.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 56 - 143 5.5e-14 B3 DNA binding domain
B3 PF02362 260 - 343 1.7e-15 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 455
Acc36I ACCTGC 1 cut(s) 303
AccI GTMKAC 1 cut(s) 123
AccII CGCG 1 cut(s) 108
AcsI RAATTY 5 cut(s) 39, 400, 407, 691, 816
AcuI CTGAAG 1 cut(s) 763
AfaI GTAC 2 cut(s) 246, 835
AfiI CCNNNNNNNGG 1 cut(s) 264
AflIII ACRYGT 1 cut(s) 656
AgsI TTSAA 6 cut(s) 172, 251, 405, 647, 884, 891
AhlI ACTAGT 1 cut(s) 602
AloI GAACNNNNNNTCC 2 cut(s) 84, 116
AluBI AGCT 7 cut(s) 21, 202, 367, 587, 875, 880, 1009
AluI AGCT 7 cut(s) 21, 202, 367, 587, 875, 880, 1009
Alw26I GTCTC 2 cut(s) 58, 695
ApeKI GCWGC 2 cut(s) 236, 746
ApoI RAATTY 5 cut(s) 39, 400, 407, 691, 816
AseI ATTAAT 1 cut(s) 918
Asp700I GAANNNNTTC 1 cut(s) 160
AspS9I GGNCC 1 cut(s) 267
AsuHPI GGTGA 2 cut(s) 197, 602
AvaII GGWCC 1 cut(s) 267
BbsI GAAGAC 2 cut(s) 117, 436
BbvI GCAGC 2 cut(s) 223, 758
BccI CCATC 3 cut(s) 267, 553, 777
BceAI ACGGC 1 cut(s) 325
BcoDI GTCTC 2 cut(s) 58, 695
BcuI ACTAGT 1 cut(s) 602
BfaI CTAG 5 cut(s) 35, 465, 603, 696, 740
BfmI CTRYAG 1 cut(s) 22
BfuAI ACCTGC 1 cut(s) 303
BisI GCNGC 2 cut(s) 237, 747
BlpI GCTNAGC 1 cut(s) 876
BlsI GCNGC 2 cut(s) 238, 748
Bme18I GGWCC 1 cut(s) 267
BmgT120I GGNCC 1 cut(s) 267
BmiI GGNNCC 1 cut(s) 269
BmrI ACTGGG 1 cut(s) 79
BmuI ACTGGG 1 cut(s) 79
BpiI GAAGAC 2 cut(s) 117, 436
Bpu1102I GCTNAGC 1 cut(s) 876
BpuEI CTTGAG 3 cut(s) 705, 916, 930
BsaBI GATNNNNATC 1 cut(s) 760
BsaXI ACNNNNNCTCC 2 cut(s) 84, 114
Bsc4I CCNNNNNNNGG 1 cut(s) 264
Bse118I RCCGGY 1 cut(s) 460
Bse1I ACTGG 1 cut(s) 74
Bse8I GATNNNNATC 1 cut(s) 760
BseGI GGATG 3 cut(s) 8, 544, 769
BseJI GATNNNNATC 1 cut(s) 760
BseLI CCNNNNNNNGG 1 cut(s) 264
BseMII CTCAG 2 cut(s) 194, 867
BseNI ACTGG 1 cut(s) 74
BseXI GCAGC 2 cut(s) 223, 758
Bsh1236I CGCG 1 cut(s) 108
BsiSI CCGG 1 cut(s) 461
BslI CCNNNNNNNGG 1 cut(s) 264
BsmAI GTCTC 2 cut(s) 58, 695
Bsp143I GATC 1 cut(s) 976
Bsp1720I GCTNAGC 1 cut(s) 876
Bsp68I TCGCGA 1 cut(s) 108
BspCNI CTCAG 2 cut(s) 195, 868
BspFNI CGCG 1 cut(s) 108
BspLI GGNNCC 1 cut(s) 269
BspMI ACCTGC 1 cut(s) 303
BsrFI RCCGGY 1 cut(s) 460
BsrI ACTGG 1 cut(s) 74
BssAI RCCGGY 1 cut(s) 460
BssMI GATC 1 cut(s) 976
Bst4CI ACNGT 1 cut(s) 500
BstAPI GCANNNNNTGC 1 cut(s) 346
BstC8I GCNNGC 1 cut(s) 462
BstDEI CTNAG 2 cut(s) 203, 876
BstF5I GGATG 3 cut(s) 8, 544, 769
BstFNI CGCG 1 cut(s) 108
BstKTI GATC 1 cut(s) 979
BstMAI GTCTC 2 cut(s) 58, 695
BstMBI GATC 1 cut(s) 976
BstMWI GCNNNNNNNGC 3 cut(s) 309, 346, 1015
BstNSI RCATGY 1 cut(s) 660
BstSFI CTRYAG 1 cut(s) 22
BstUI CGCG 1 cut(s) 108
BstV1I GCAGC 2 cut(s) 223, 758
BstV2I GAAGAC 2 cut(s) 117, 436
BtsCI GGATG 3 cut(s) 8, 544, 769
BtsIMutI CAGTG 1 cut(s) 67
BtuMI TCGCGA 1 cut(s) 108
BveI ACCTGC 1 cut(s) 303
Cac8I GCNNGC 1 cut(s) 462
Cfr10I RCCGGY 1 cut(s) 460
Cfr13I GGNCC 1 cut(s) 267
Csp6I GTAC 2 cut(s) 245, 834
CviAII CATG 5 cut(s) 142, 350, 434, 657, 831
CviQI GTAC 2 cut(s) 245, 834
DdeI CTNAG 2 cut(s) 203, 876
DpnI GATC 1 cut(s) 978
DpnII GATC 1 cut(s) 976
DraI TTTAAA 2 cut(s) 363, 487
Eco32I GATATC 1 cut(s) 31
Eco47I GGWCC 1 cut(s) 267
Eco57I CTGAAG 1 cut(s) 763
EcoRI GAATTC 2 cut(s) 691, 816
EcoRV GATATC 1 cut(s) 31
FaeI CATG 5 cut(s) 145, 353, 437, 660, 834
FalI AAGNNNNNCTT 4 cut(s) 145, 177, 149, 181
FatI CATG 5 cut(s) 141, 349, 433, 656, 830
FblI GTMKAC 1 cut(s) 123
Fnu4HI GCNGC 2 cut(s) 237, 747
FokI GGATG 2 cut(s) 531, 756
Fsp4HI GCNGC 2 cut(s) 237, 747
FspBI CTAG 5 cut(s) 35, 465, 603, 696, 740
GluI GCNGC 2 cut(s) 237, 747
HapII CCGG 1 cut(s) 461
Hin1II CATG 5 cut(s) 145, 353, 437, 660, 834
HincII GTYRAC 1 cut(s) 418
HindII GTYRAC 1 cut(s) 418
HinfI GANTC 5 cut(s) 86, 207, 674, 923, 939
HpaII CCGG 1 cut(s) 461
HphI GGTGA 2 cut(s) 197, 602
Hpy166II GTNNAC 3 cut(s) 124, 245, 418
Hpy188III TCNNGA 4 cut(s) 107, 608, 696, 740
Hpy8I GTNNAC 3 cut(s) 124, 245, 418
Hpy99I CGWCG 1 cut(s) 108
HpyAV CCTTC 1 cut(s) 171
HpyCH4III ACNGT 1 cut(s) 500
HpyCH4IV ACGT 1 cut(s) 1060
HpyCH4V TGCA 4 cut(s) 239, 349, 441, 823
HpyF10VI GCNNNNNNNGC 3 cut(s) 309, 346, 1015
HpyF3I CTNAG 2 cut(s) 203, 876
HpySE526I ACGT 1 cut(s) 1060
Hsp92II CATG 5 cut(s) 145, 353, 437, 660, 834
KroI GCCGGC 1 cut(s) 460
KroNI GCCGGC 1 cut(s) 462
Kzo9I GATC 1 cut(s) 976
LmnI GCTCC 1 cut(s) 105
Lsp1109I GCAGC 2 cut(s) 223, 758
MaeI CTAG 5 cut(s) 35, 465, 603, 696, 740
MaeII ACGT 1 cut(s) 1060
MaeIII GTNAC 2 cut(s) 185, 805
MalI GATC 1 cut(s) 978
MboI GATC 1 cut(s) 976
MboII GAAGA 5 cut(s) 122, 254, 441, 565, 568
MfeI CAATTG 1 cut(s) 636
MlyI GAGTC 2 cut(s) 683, 917
MmeI TCCRAC 3 cut(s) 314, 739, 932
MnlI CCTC 3 cut(s) 16, 486, 729
MroNI GCCGGC 1 cut(s) 460
MroXI GAANNNNTTC 1 cut(s) 160
MseI TTAA 5 cut(s) 362, 486, 855, 918, 1014
MslI CAYNNNNRTG 2 cut(s) 354, 501
MspA1I CMGCKG 2 cut(s) 587, 875
MspI CCGG 1 cut(s) 461
MunI CAATTG 1 cut(s) 636
MvnI CGCG 1 cut(s) 108
MwoI GCNNNNNNNGC 3 cut(s) 309, 346, 1015
NaeI GCCGGC 1 cut(s) 462
NdeII GATC 1 cut(s) 976
NgoMIV GCCGGC 1 cut(s) 460
NlaIII CATG 5 cut(s) 145, 353, 437, 660, 834
NlaIV GGNNCC 1 cut(s) 269
NmuCI GTSAC 2 cut(s) 185, 805
NruI TCGCGA 1 cut(s) 108
NspI RCATGY 1 cut(s) 660
PciI ACATGT 1 cut(s) 656
PdiI GCCGGC 1 cut(s) 462
PdmI GAANNNNTTC 1 cut(s) 160
PfeI GAWTC 3 cut(s) 86, 207, 939
PflFI GACNNNGTC 1 cut(s) 706
PkrI GCNGC 2 cut(s) 238, 748
PleI GAGTC 2 cut(s) 682, 917
PpsI GAGTC 2 cut(s) 682, 917
PscI ACATGT 1 cut(s) 656
PshBI ATTAAT 1 cut(s) 918
PsiI TTATAA 1 cut(s) 455
PspN4I GGNNCC 1 cut(s) 269
PspPI GGNCC 1 cut(s) 267
PsyI GACNNNGTC 1 cut(s) 706
PvuII CAGCTG 2 cut(s) 587, 875
RruI TCGCGA 1 cut(s) 108
RsaI GTAC 2 cut(s) 246, 835
RsaNI GTAC 2 cut(s) 245, 834
RseI CAYNNNNRTG 2 cut(s) 354, 501
SaqAI TTAA 5 cut(s) 362, 486, 855, 918, 1014
SatI GCNGC 2 cut(s) 237, 747
Sau3AI GATC 1 cut(s) 976
Sau96I GGNCC 1 cut(s) 267
SchI GAGTC 2 cut(s) 683, 917
SfcI CTRYAG 1 cut(s) 22
SinI GGWCC 1 cut(s) 267
SmiMI CAYNNNNRTG 2 cut(s) 354, 501
SmlI CTYRAG 3 cut(s) 720, 931, 945
SmoI CTYRAG 3 cut(s) 720, 931, 945
SpeI ACTAGT 1 cut(s) 602
SspMI CTAG 5 cut(s) 35, 465, 603, 696, 740
TaaI ACNGT 1 cut(s) 500
TaiI ACGT 1 cut(s) 1063
TaqI TCGA 2 cut(s) 291, 764
TaqII GACCGA 1 cut(s) 809
TatI WGTACW 1 cut(s) 244
TfiI GAWTC 3 cut(s) 86, 207, 939
Tru1I TTAA 5 cut(s) 362, 486, 855, 918, 1014
Tru9I TTAA 5 cut(s) 362, 486, 855, 918, 1014
TscAI CASTG 1 cut(s) 74
TseFI GTSAC 2 cut(s) 185, 805
TseI GCWGC 2 cut(s) 236, 746
Tsp45I GTSAC 2 cut(s) 185, 805
TspDTI ATGAA 3 cut(s) 519, 704, 1055
TspGWI ACGGA 2 cut(s) 92, 610
TspRI CASTG 1 cut(s) 74
Tth111I GACNNNGTC 1 cut(s) 706
VpaK11BI GGWCC 1 cut(s) 267
VspI ATTAAT 1 cut(s) 918
XapI RAATTY 5 cut(s) 39, 400, 407, 691, 816
XbaI TCTAGA 2 cut(s) 695, 739
XceI RCATGY 1 cut(s) 660
XmiI GTMKAC 1 cut(s) 123
XmnI GAANNNNTTC 1 cut(s) 160
XspI CTAG 5 cut(s) 35, 465, 603, 696, 740
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.