Rmu_sc0021123.1_g000001

B3 DNA binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0021123.1
OG0000593
Physical Location & Seq
Reverse (-)
1012 .. 1888
877 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0021123.1_g000001.1.cds

Sequence Viewer

Length: 699 bp
atgaagtatggacttgcaaggtatcccttttttgacatgaagttccttgtgactattggagagtatgcttttgggagaatcccacaggcttttcgtaagcatttggctgcaagtgtacttcaacaatcccatcttcggacccctgctggaacttggcttgttgatgtgaaaagcgataacggcaggttctttttacaaaagggttggaagcaatttgtgcatgataatggtttaaagcttggagagtttctaattttccgatatgctggaaattcaaaattctatgttgacatatatggaagacatggttgcaaaaaggagtttgtaatgccggctaggagaagtgaaaaaccatttaaaaagaggcacggtaatgaaagtcatagaacaaacgctgttatgggttccagcatacatccaaagaagaagatggattatcagaagttaaaaggttcagctggtgaaaataaaactagtcaagaaactatgtccgtcaaatctgaaccaattgattttgaattggaaacatctatggatattgacgagtcaattagaaatatgaattctagagagactatgtctttccagcctcaagtggcaaatagtgctctagaagcagccaacagatactcatcgaaccatccttgcttcagaactcttttgaccgaaacttatgtgaccaaaggaattctggtatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

232

Amino Acids

26.65

Weight (kDa)

9.72

Isoelectric Point (pI)

40.17

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 174
AcsI RAATTY 4 cut(s) 271, 278, 562, 687
AcuI CTGAAG 1 cut(s) 634
AfaI GTAC 1 cut(s) 117
AfiI CCNNNNNNNGG 1 cut(s) 135
AgsI TTSAA 3 cut(s) 122, 276, 518
AhlI ACTAGT 1 cut(s) 473
AluBI AGCT 2 cut(s) 238, 458
AluI AGCT 2 cut(s) 238, 458
Alw21I GWGCWC 1 cut(s) 610
Alw26I GTCTC 1 cut(s) 566
ApeKI GCWGC 2 cut(s) 107, 617
ApoI RAATTY 4 cut(s) 271, 278, 562, 687
AspS9I GGNCC 1 cut(s) 138
AsuHPI GGTGA 1 cut(s) 473
AvaII GGWCC 1 cut(s) 138
BbsI GAAGAC 1 cut(s) 307
Bbv12I GWGCWC 1 cut(s) 610
BbvI GCAGC 2 cut(s) 94, 629
BccI CCATC 3 cut(s) 138, 424, 648
BceAI ACGGC 1 cut(s) 196
BciVI GTATCC 1 cut(s) 33
BcoDI GTCTC 1 cut(s) 566
BcuI ACTAGT 1 cut(s) 473
BfaI CTAG 4 cut(s) 336, 474, 567, 611
BfuAI ACCTGC 1 cut(s) 174
BfuI GTATCC 1 cut(s) 33
BisI GCNGC 2 cut(s) 108, 618
BlsI GCNGC 2 cut(s) 109, 619
Bme18I GGWCC 1 cut(s) 138
BmgT120I GGNCC 1 cut(s) 138
BmiI GGNNCC 2 cut(s) 140, 406
BpiI GAAGAC 1 cut(s) 307
BpuEI CTTGAG 1 cut(s) 576
BsaBI GATNNNNATC 1 cut(s) 631
Bsc4I CCNNNNNNNGG 1 cut(s) 135
Bse118I RCCGGY 1 cut(s) 331
Bse8I GATNNNNATC 1 cut(s) 631
BseGI GGATG 2 cut(s) 415, 640
BseJI GATNNNNATC 1 cut(s) 631
BseLI CCNNNNNNNGG 1 cut(s) 135
BseXI GCAGC 2 cut(s) 94, 629
BsiHKAI GWGCWC 1 cut(s) 610
BsiSI CCGG 1 cut(s) 332
BslI CCNNNNNNNGG 1 cut(s) 135
BsmAI GTCTC 1 cut(s) 566
Bsp1286I GDGCHC 1 cut(s) 610
BspLI GGNNCC 2 cut(s) 140, 406
BspMI ACCTGC 1 cut(s) 174
BsrFI RCCGGY 1 cut(s) 331
BssAI RCCGGY 1 cut(s) 331
Bst4CI ACNGT 1 cut(s) 371
BstAPI GCANNNNNTGC 2 cut(s) 217, 605
BstC8I GCNNGC 1 cut(s) 333
BstF5I GGATG 2 cut(s) 415, 640
BstMAI GTCTC 1 cut(s) 566
BstMWI GCNNNNNNNGC 4 cut(s) 180, 217, 605, 614
BstV1I GCAGC 2 cut(s) 94, 629
BstV2I GAAGAC 1 cut(s) 307
BsuI GTATCC 1 cut(s) 33
BtsCI GGATG 2 cut(s) 415, 640
BveI ACCTGC 1 cut(s) 174
Cac8I GCNNGC 1 cut(s) 333
Cfr10I RCCGGY 1 cut(s) 331
Cfr13I GGNCC 1 cut(s) 138
Csp6I GTAC 1 cut(s) 116
CviAII CATG 3 cut(s) 37, 221, 305
CviJI RGCY 8 cut(s) 89, 107, 157, 238, 335, 458, 589, 620
CviKI_1 RGCY 8 cut(s) 89, 107, 157, 238, 335, 458, 589, 620
CviQI GTAC 1 cut(s) 116
DraI TTTAAA 2 cut(s) 234, 358
Eco47I GGWCC 1 cut(s) 138
Eco57I CTGAAG 1 cut(s) 634
EcoRI GAATTC 2 cut(s) 562, 687
FaeI CATG 3 cut(s) 40, 224, 308
FatI CATG 3 cut(s) 36, 220, 304
Fnu4HI GCNGC 2 cut(s) 108, 618
FokI GGATG 2 cut(s) 402, 627
Fsp4HI GCNGC 2 cut(s) 108, 618
FspBI CTAG 4 cut(s) 336, 474, 567, 611
GluI GCNGC 2 cut(s) 108, 618
HapII CCGG 1 cut(s) 332
Hin1II CATG 3 cut(s) 40, 224, 308
HincII GTYRAC 1 cut(s) 289
HindII GTYRAC 1 cut(s) 289
HindIII AAGCTT 1 cut(s) 236
HinfI GANTC 2 cut(s) 78, 545
HpaII CCGG 1 cut(s) 332
HphI GGTGA 1 cut(s) 473
Hpy166II GTNNAC 2 cut(s) 116, 289
Hpy188I TCNGA 5 cut(s) 138, 260, 441, 502, 653
Hpy188III TCNNGA 3 cut(s) 479, 567, 611
Hpy8I GTNNAC 2 cut(s) 116, 289
HpyCH4III ACNGT 1 cut(s) 371
HpyCH4V TGCA 4 cut(s) 17, 110, 220, 312
HpyF10VI GCNNNNNNNGC 4 cut(s) 180, 217, 605, 614
Hsp92II CATG 3 cut(s) 40, 224, 308
KroI GCCGGC 1 cut(s) 331
KroNI GCCGGC 1 cut(s) 333
Lsp1109I GCAGC 2 cut(s) 94, 629
MaeI CTAG 4 cut(s) 336, 474, 567, 611
MaeIII GTNAC 2 cut(s) 49, 676
MboII GAAGA 4 cut(s) 125, 312, 436, 439
MfeI CAATTG 1 cut(s) 507
MhlI GDGCHC 1 cut(s) 610
MluCI AATT 9 cut(s) 212, 252, 271, 278, 507, 518, 549, 562, 687
MlyI GAGTC 1 cut(s) 554
MmeI TCCRAC 1 cut(s) 185
MnlI CCTC 2 cut(s) 357, 600
MroNI GCCGGC 1 cut(s) 331
MseI TTAA 3 cut(s) 233, 357, 446
MslI CAYNNNNRTG 2 cut(s) 225, 372
MspA1I CMGCKG 1 cut(s) 458
MspI CCGG 1 cut(s) 332
MunI CAATTG 1 cut(s) 507
MwoI GCNNNNNNNGC 4 cut(s) 180, 217, 605, 614
NaeI GCCGGC 1 cut(s) 333
NgoMIV GCCGGC 1 cut(s) 331
NlaIII CATG 3 cut(s) 40, 224, 308
NlaIV GGNNCC 2 cut(s) 140, 406
NmuCI GTSAC 2 cut(s) 49, 676
PdiI GCCGGC 1 cut(s) 333
PfeI GAWTC 1 cut(s) 78
PflFI GACNNNGTC 1 cut(s) 577
PkrI GCNGC 2 cut(s) 109, 619
PleI GAGTC 1 cut(s) 553
PpsI GAGTC 1 cut(s) 553
PspN4I GGNNCC 2 cut(s) 140, 406
PspPI GGNCC 1 cut(s) 138
PsyI GACNNNGTC 1 cut(s) 577
PvuII CAGCTG 1 cut(s) 458
RsaI GTAC 1 cut(s) 117
RsaNI GTAC 1 cut(s) 116
RseI CAYNNNNRTG 2 cut(s) 225, 372
SaqAI TTAA 3 cut(s) 233, 357, 446
SatI GCNGC 2 cut(s) 108, 618
Sau96I GGNCC 1 cut(s) 138
SchI GAGTC 1 cut(s) 554
SduI GDGCHC 1 cut(s) 610
SetI ASST 5 cut(s) 23, 188, 240, 454, 460
SinI GGWCC 1 cut(s) 138
SmiMI CAYNNNNRTG 2 cut(s) 225, 372
SmlI CTYRAG 1 cut(s) 591
SmoI CTYRAG 1 cut(s) 591
SpeI ACTAGT 1 cut(s) 473
Sse9I AATT 9 cut(s) 212, 252, 271, 278, 507, 518, 549, 562, 687
SspMI CTAG 4 cut(s) 336, 474, 567, 611
TaaI ACNGT 1 cut(s) 371
TaqI TCGA 1 cut(s) 635
TaqII GACCGA 1 cut(s) 680
TasI AATT 9 cut(s) 212, 252, 271, 278, 507, 518, 549, 562, 687
TatI WGTACW 1 cut(s) 115
TfiI GAWTC 1 cut(s) 78
Tru1I TTAA 3 cut(s) 233, 357, 446
Tru9I TTAA 3 cut(s) 233, 357, 446
TseFI GTSAC 2 cut(s) 49, 676
TseI GCWGC 2 cut(s) 107, 617
Tsp45I GTSAC 2 cut(s) 49, 676
TspDTI ATGAA 4 cut(s) 17, 53, 390, 575
TspGWI ACGGA 1 cut(s) 481
Tth111I GACNNNGTC 1 cut(s) 577
VpaK11BI GGWCC 1 cut(s) 138
XapI RAATTY 4 cut(s) 271, 278, 562, 687
XbaI TCTAGA 2 cut(s) 566, 610
XcmI CCANNNNNNNNNTGG 1 cut(s) 688
XspI CTAG 4 cut(s) 336, 474, 567, 611
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.