Prupe.7G144000_v2.0.a1

B3 DNA binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp07
OG0000593
Physical Location & Seq
Forward (+)
15798910 .. 15801700
2791 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.7G144000.1

Sequence Viewer

Length: 969 bp
ATGGCAAGAAAATCAAGTGCAGTAAGAAGAAAACCCTCATTTTTCAAGGCTCTTGTAGGCAACTTCTCTAACCAGCTGCAAATCCCGCCAGCATTTCTTGTGCATTTTGATGGTGGAAAAGTGCCTCAGAAGTCCCACCTTCGGACCTCGGCTGGAACTTGGCCTGTTAATGTGAAGAAGGCTGATGACAAGTTCTTTTTCCAAAAGGGTTGGAAGAAATTTGTGCATGACAATGATTTAAAGCTTTGTGAATTTCTAGTCTTTGGATGTGCTGGAGATTTGGGATTCTATGTTGACATTTATGGAAGAAATGGCGGCAAAAGAGAGTTTGTAACGGCCGAAAGGGACAGACCTCATGAAGAGGGACATGGTAATGTATTTCACAGAAAGAGCCCAAGACATCATGGAAAACAGAATTTGCCTTCAATTGATCCCATCAAATATGAAGCAACCGATACTGAATCTGAAACATCAATGCATCCATTGGCTACAACAAGTACTGGAAAAACTATTGCTCTTCAGAGAGACAATAGCTTCAACTCTGAAAAACCTTTTTTCAAGGTTGCATTATGGAACTCGTACATGGATCGAGGTGTGATGTGTTTGCCAAAAAACTTTGTCAAGACGCATCTCACCAGGCACCGAGCTAATGTTACCCTTCGGGTTTCGGATGGGAGAACTTGGCCTGTCAATTTGATATTTGAAGGTGAAAGAGGCAAACTCGGTGGTGGTTTCATGGCATTTTGCCGAGATAATAATTTAAAAGTTGGTGACATGTGTGTCTTTGTCTTGATTAACAAGATTGAGTTTTTGTTTGAAGCTGGCTTTTACCGCAAGACAGAAGCTGCAAATTGCACCGTGCCACCAGAACATTATGTTATGGATATCACGGGGTGTAGCGACATATGTTTTTATAAACTGAAGGAACAGATTGCCACTCTTCAGGTCAGAGATATACCAGGCATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

323

Amino Acids

36.6

Weight (kDa)

9.36

Isoelectric Point (pI)

33.25

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 915
AasI GACNNNNNNGTC 1 cut(s) 779
AccB1I GGYRCC 1 cut(s) 639
AciI CCGC 3 cut(s) 86, 315, 832
AclWI GGATC 2 cut(s) 425, 594
AcoI YGGCCR 1 cut(s) 336
AcsI RAATTY 3 cut(s) 218, 251, 415
AcuI CTGAAG 3 cut(s) 503, 926, 941
AdeI CACNNNGTG 1 cut(s) 894
AfaI GTAC 2 cut(s) 499, 581
AfiI CCNNNNNNNGG 1 cut(s) 141
AflIII ACRYGT 1 cut(s) 774
AgsI TTSAA 6 cut(s) 46, 426, 538, 559, 704, 818
AjnI CCWGG 2 cut(s) 635, 958
AluBI AGCT 6 cut(s) 76, 244, 534, 647, 821, 845
AluI AGCT 6 cut(s) 76, 244, 534, 647, 821, 845
Alw26I GTCTC 1 cut(s) 519
AlwI GGATC 2 cut(s) 425, 594
AlwNI CAGNNNCTG 1 cut(s) 845
AoxI GGCC 3 cut(s) 161, 336, 683
ApeKI GCWGC 2 cut(s) 76, 845
ApoI RAATTY 3 cut(s) 218, 251, 415
AspS9I GGNCC 1 cut(s) 144
AsuHPI GGTGA 3 cut(s) 625, 719, 782
AvaII GGWCC 1 cut(s) 144
BanI GGYRCC 1 cut(s) 639
BanII GRGCYC 1 cut(s) 395
BbvI GCAGC 2 cut(s) 63, 832
BccI CCATC 3 cut(s) 104, 443, 665
BceAI ACGGC 1 cut(s) 351
BciT130I CCWGG 2 cut(s) 637, 960
BcoDI GTCTC 1 cut(s) 519
BfaI CTAG 1 cut(s) 257
BisI GCNGC 3 cut(s) 77, 316, 846
BlsI GCNGC 3 cut(s) 78, 317, 847
BmcAI AGTACT 1 cut(s) 499
Bme1390I CCNGG 2 cut(s) 637, 960
Bme18I GGWCC 1 cut(s) 144
BmgT120I GGNCC 1 cut(s) 144
BmiI GGNNCC 1 cut(s) 641
BmrFI CCNGG 2 cut(s) 637, 960
BmsI GCATC 2 cut(s) 487, 637
BplI GAGNNNNNCTC 2 cut(s) 705, 737
BpmI CTGGAG 1 cut(s) 294
BsaJI CCNNGG 1 cut(s) 147
Bsc4I CCNNNNNNNGG 1 cut(s) 141
Bse1I ACTGG 1 cut(s) 505
BseBI CCWGG 2 cut(s) 637, 960
BseDI CCNNGG 1 cut(s) 147
BseGI GGATG 3 cut(s) 272, 478, 676
BseLI CCNNNNNNNGG 1 cut(s) 141
BseMII CTCAG 1 cut(s) 140
BseNI ACTGG 1 cut(s) 505
BseX3I CGGCCG 1 cut(s) 336
BseXI GCAGC 2 cut(s) 63, 832
BsgI GTGCAG 1 cut(s) 39
Bsh1285I CGRYCG 1 cut(s) 339
BshFI GGCC 3 cut(s) 163, 338, 685
BshNI GGYRCC 1 cut(s) 639
BsiEI CGRYCG 1 cut(s) 339
BslFI GGGAC 3 cut(s) 118, 359, 378
BslI CCNNNNNNNGG 1 cut(s) 141
BsmAI GTCTC 1 cut(s) 519
BsmFI GGGAC 3 cut(s) 118, 359, 378
BsnI GGCC 3 cut(s) 163, 338, 685
Bsp1286I GDGCHC 1 cut(s) 395
Bsp143I GATC 2 cut(s) 430, 586
BspACI CCGC 3 cut(s) 86, 315, 832
BspANI GGCC 3 cut(s) 163, 338, 685
BspCNI CTCAG 1 cut(s) 139
BspHI TCATGA 1 cut(s) 355
BspLI GGNNCC 1 cut(s) 641
BspPI GGATC 2 cut(s) 425, 594
BspQI GCTCTTC 1 cut(s) 522
BspT107I GGYRCC 1 cut(s) 639
BsrI ACTGG 1 cut(s) 505
BssECI CCNNGG 1 cut(s) 147
BssMI GATC 2 cut(s) 430, 586
Bst2UI CCWGG 2 cut(s) 637, 960
Bst4CI ACNGT 1 cut(s) 859
Bst6I CTCTTC 3 cut(s) 354, 522, 945
BstC8I GCNNGC 2 cut(s) 90, 823
BstDEI CTNAG 1 cut(s) 126
BstF5I GGATG 3 cut(s) 272, 478, 676
BstKTI GATC 2 cut(s) 433, 589
BstMAI GTCTC 1 cut(s) 519
BstMBI GATC 2 cut(s) 430, 586
BstMCI CGRYCG 1 cut(s) 339
BstMWI GCNNNNNNNGC 2 cut(s) 85, 831
BstNI CCWGG 2 cut(s) 637, 960
BstNSI RCATGY 2 cut(s) 778, 967
BstSCI CCNGG 2 cut(s) 635, 958
BstV1I GCAGC 2 cut(s) 63, 832
BstZI CGGCCG 1 cut(s) 336
BsuRI GGCC 3 cut(s) 163, 338, 685
BtsCI GGATG 3 cut(s) 272, 478, 676
Cac8I GCNNGC 2 cut(s) 90, 823
CaiI CAGNNNCTG 1 cut(s) 845
CciI TCATGA 1 cut(s) 355
Cfr13I GGNCC 1 cut(s) 144
CseI GACGC 1 cut(s) 634
Csp6I GTAC 2 cut(s) 498, 580
CspCI CAANNNNNGTGG 2 cut(s) 706, 741
CviAII CATG 8 cut(s) 227, 356, 368, 404, 583, 736, 775, 964
CviQI GTAC 2 cut(s) 498, 580
DdeI CTNAG 1 cut(s) 126
DpnI GATC 2 cut(s) 432, 588
DpnII GATC 2 cut(s) 430, 586
DraI TTTAAA 2 cut(s) 240, 762
DraIII CACNNNGTG 1 cut(s) 894
DrdI GACNNNNNNGTC 1 cut(s) 779
DseDI GACNNNNNNGTC 1 cut(s) 779
EaeI YGGCCR 1 cut(s) 336
EagI CGGCCG 1 cut(s) 336
Eam1104I CTCTTC 3 cut(s) 354, 522, 945
EarI CTCTTC 3 cut(s) 354, 522, 945
EclXI CGGCCG 1 cut(s) 336
Eco24I GRGCYC 1 cut(s) 395
Eco32I GATATC 1 cut(s) 886
Eco47I GGWCC 1 cut(s) 144
Eco52I CGGCCG 1 cut(s) 336
Eco57I CTGAAG 3 cut(s) 503, 926, 941
EcoRII CCWGG 2 cut(s) 635, 958
EcoRV GATATC 1 cut(s) 886
EcoT22I ATGCAT 1 cut(s) 480
EcoT38I GRGCYC 1 cut(s) 395
FaeI CATG 8 cut(s) 230, 359, 371, 407, 586, 739, 778, 967
FaqI GGGAC 3 cut(s) 118, 359, 378
FatI CATG 8 cut(s) 226, 355, 367, 403, 582, 735, 774, 963
FauI CCCGC 1 cut(s) 93
FauNDI CATATG 1 cut(s) 905
Fnu4HI GCNGC 3 cut(s) 77, 316, 846
FokI GGATG 3 cut(s) 279, 465, 683
FriOI GRGCYC 1 cut(s) 395
Fsp4HI GCNGC 3 cut(s) 77, 316, 846
FspBI CTAG 1 cut(s) 257
GluI GCNGC 3 cut(s) 77, 316, 846
GsuI CTGGAG 1 cut(s) 294
HaeIII GGCC 3 cut(s) 163, 338, 685
HgaI GACGC 1 cut(s) 634
Hin1II CATG 8 cut(s) 230, 359, 371, 407, 586, 739, 778, 967
HincII GTYRAC 1 cut(s) 295
HindII GTYRAC 1 cut(s) 295
HindIII AAGCTT 1 cut(s) 242
HinfI GANTC 2 cut(s) 285, 461
HphI GGTGA 3 cut(s) 625, 719, 782
Hpy166II GTNNAC 1 cut(s) 295
Hpy188I TCNGA 7 cut(s) 129, 144, 466, 522, 544, 670, 950
Hpy188III TCNNGA 3 cut(s) 356, 622, 790
Hpy8I GTNNAC 1 cut(s) 295
HpyAV CCTTC 6 cut(s) 149, 172, 432, 668, 698, 916
HpyCH4III ACNGT 1 cut(s) 859
HpyCH4V TGCA 8 cut(s) 20, 79, 103, 226, 478, 566, 848, 855
HpyF10VI GCNNNNNNNGC 2 cut(s) 85, 831
HpyF3I CTNAG 1 cut(s) 126
Hsp92II CATG 8 cut(s) 230, 359, 371, 407, 586, 739, 778, 967
Kzo9I GATC 2 cut(s) 430, 586
LguI GCTCTTC 1 cut(s) 522
Lsp1109I GCAGC 2 cut(s) 63, 832
LweI GCATC 2 cut(s) 487, 637
MaeI CTAG 1 cut(s) 257
MaeIII GTNAC 3 cut(s) 331, 652, 770
MalI GATC 2 cut(s) 432, 588
MboI GATC 2 cut(s) 430, 586
MboII GAAGA 7 cut(s) 39, 187, 226, 318, 371, 509, 932
MfeI CAATTG 1 cut(s) 426
MhlI GDGCHC 1 cut(s) 395
MluCI AATT 7 cut(s) 218, 251, 415, 426, 691, 757, 850
MmeI TCCRAC 1 cut(s) 191
MnlI CCTC 7 cut(s) 46, 135, 157, 355, 363, 584, 707
Mph1103I ATGCAT 1 cut(s) 480
MseI TTAA 4 cut(s) 168, 239, 761, 795
MslI CAYNNNNRTG 3 cut(s) 108, 231, 372
MspA1I CMGCKG 1 cut(s) 76
MspR9I CCNGG 2 cut(s) 637, 960
MunI CAATTG 1 cut(s) 426
MvaI CCWGG 2 cut(s) 637, 960
MwoI GCNNNNNNNGC 2 cut(s) 85, 831
NdeI CATATG 1 cut(s) 905
NdeII GATC 2 cut(s) 430, 586
NlaIII CATG 8 cut(s) 230, 359, 371, 407, 586, 739, 778, 967
NlaIV GGNNCC 1 cut(s) 641
NmeAIII GCCGAG 2 cut(s) 128, 773
NmuCI GTSAC 1 cut(s) 770
NsiI ATGCAT 1 cut(s) 480
NspI RCATGY 2 cut(s) 778, 967
PagI TCATGA 1 cut(s) 355
PciI ACATGT 1 cut(s) 774
PciSI GCTCTTC 1 cut(s) 522
PfeI GAWTC 2 cut(s) 285, 461
PkrI GCNGC 3 cut(s) 78, 317, 847
PscI ACATGT 1 cut(s) 774
PsiI TTATAA 1 cut(s) 915
Psp6I CCWGG 2 cut(s) 635, 958
PspGI CCWGG 2 cut(s) 635, 958
PspN4I GGNNCC 1 cut(s) 641
PspPI GGNCC 1 cut(s) 144
PstNI CAGNNNCTG 1 cut(s) 845
PvuII CAGCTG 1 cut(s) 76
RsaI GTAC 2 cut(s) 499, 581
RsaNI GTAC 2 cut(s) 498, 580
RseI CAYNNNNRTG 3 cut(s) 108, 231, 372
SapI GCTCTTC 1 cut(s) 522
SaqAI TTAA 4 cut(s) 168, 239, 761, 795
SatI GCNGC 3 cut(s) 77, 316, 846
Sau3AI GATC 2 cut(s) 430, 586
Sau96I GGNCC 1 cut(s) 144
ScaI AGTACT 1 cut(s) 499
ScrFI CCNGG 2 cut(s) 637, 960
SduI GDGCHC 1 cut(s) 395
SfaNI GCATC 2 cut(s) 487, 637
SinI GGWCC 1 cut(s) 144
SmiMI CAYNNNNRTG 3 cut(s) 108, 231, 372
Sse9I AATT 7 cut(s) 218, 251, 415, 426, 691, 757, 850
SsiI CCGC 3 cut(s) 86, 315, 832
SspMI CTAG 1 cut(s) 257
StyD4I CCNGG 2 cut(s) 635, 958
TaaI ACNGT 1 cut(s) 859
TaqI TCGA 1 cut(s) 589
TasI AATT 7 cut(s) 218, 251, 415, 426, 691, 757, 850
TatI WGTACW 1 cut(s) 497
TauI GCSGC 1 cut(s) 318
TfiI GAWTC 2 cut(s) 285, 461
Tru1I TTAA 4 cut(s) 168, 239, 761, 795
Tru9I TTAA 4 cut(s) 168, 239, 761, 795
TseFI GTSAC 1 cut(s) 770
TseI GCWGC 2 cut(s) 76, 845
Tsp45I GTSAC 1 cut(s) 770
TspDTI ATGAA 3 cut(s) 372, 459, 724
VpaK11BI GGWCC 1 cut(s) 144
XapI RAATTY 3 cut(s) 218, 251, 415
XceI RCATGY 2 cut(s) 778, 967
XspI CTAG 1 cut(s) 257
ZrmI AGTACT 1 cut(s) 499
Zsp2I ATGCAT 1 cut(s) 480
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.