Rw1G019210

B3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0000593
Physical Location & Seq
Forward (+)
42056252 .. 42058468
2217 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G019210.1

Sequence Viewer

Length: 1119 bp
ATGGCAAGAAGAAGGGATCCTTCTTTCTTCAAGATTCTTCTGGGGGATCACTTCTCTCACAAGTTGATAATTCCTGGAGCTTTTCTCAAACATTTTGATGGGAATGTGCCTGAACAGTTTCGTCTTCAAACACCAGCTAAAACTTGGCTTGTTGATGTGGAAAAGGTTGATCATAAGTACTATTTCCAAAAGGGTTGGAATGAATTTGTGAGTGAAAATGGCTTAAGGGTTAAGGAAATTCTAGTTTTTCATTATACTGGAAATTCAGACTTCTCTGTCGACATATATGGAAAAAATACTATCAAAAAGGTGTTTGTAAGAGAAAGTCATGCCTTCCAGGGATCAAAAGATGTCGGCATCCCCACAGACATTGACTATCCTATGGAAGAAGCTGATGAAGATGATGATCCGTCTGTTGAAATATTGGATGATGTTGCACCATACCCAACAAGTAGAGGAGAGAAATATCAGATATCAAGTCCTCTGCCTCACATTGATTATGACCTTGACATTCAAGATAACATATATGATGAGAATAAGCATGAAAGTTATAAAAATGAAGAAGATGAAAATGAGGAAGATGAAGAAGATGAAGATGAAAGTCTGAGTGAAGAAGAAAATGAAACTGCGGAAGATGTTGATTTTGGAGATGAGGATCAAAATGAAAGTGGCTCTTCCAGAACTGAAAGTTTCCGAAAACCTGAGATACCTAAGAAGATGAATCTAGCTATGGAAGTTGGAAAGCATAATGCTCTTCAGAGAGCTCTTGATTTTAAATCTAAATCTGTAAATCCTTGTCTTGTGCTTCCCATGCGTGCCTCTTATGCCCGTGGAAGTTACTTGAATTTTCCATTTGACTTTGCCAAGAGATATCTTAGGAAGCAGCCTAGTAACGTCACTTTTCTGGCCGATGGTGTATCTTGGCCTGTCAAGTTCTATTATCCCCAAAACCAAACCAGAGCAGTGGCTGGGTGGTCTACATTTGCGCGAGTCAATAAATTGAAGGAGAGTGATGTGTGTGTCTTTGTGTTGACTGACGACATCAAATTTGTATTTAGTGTCGCGATTTTCCGCATTACAGGTGCTGCAAATTGCACCTTACCATCACCAGGTGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

372

Amino Acids

42.74

Weight (kDa)

4.79

Isoelectric Point (pI)

50.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 9 - 98 1.7e-14 B3 DNA binding domain
B3 PF02362 274 - 358 7.1e-11 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 552
AasI GACNNNNNNGTC 1 cut(s) 275
AccI GTMKAC 2 cut(s) 279, 977
AccII CGCG 2 cut(s) 988, 1064
AciI CCGC 2 cut(s) 629, 1072
AclWI GGATC 6 cut(s) 11, 24, 54, 349, 401, 663
AcoI YGGCCR 1 cut(s) 906
AcsI RAATTY 5 cut(s) 203, 237, 262, 844, 1046
AcuI CTGAAG 1 cut(s) 740
AdeI CACNNNGTG 1 cut(s) 1112
AfaI GTAC 1 cut(s) 179
AfiI CCNNNNNNNGG 1 cut(s) 1109
AflII CTTAAG 1 cut(s) 223
AgsI TTSAA 6 cut(s) 31, 128, 419, 515, 844, 1003
AjnI CCWGG 3 cut(s) 73, 336, 1108
AluBI AGCT 5 cut(s) 80, 137, 392, 728, 764
AluI AGCT 5 cut(s) 80, 137, 392, 728, 764
Alw21I GWGCWC 1 cut(s) 766
AlwI GGATC 6 cut(s) 11, 24, 54, 349, 401, 663
AlwNI CAGNNNCTG 2 cut(s) 968, 1085
AoxI GGCC 2 cut(s) 906, 923
ApeKI GCWGC 2 cut(s) 883, 1085
ApoI RAATTY 5 cut(s) 203, 237, 262, 844, 1046
ArsI GACNNNNNNTTYG 2 cut(s) 1031, 1063
Asp700I GAANNNNTTC 1 cut(s) 117
AspLEI GCGC 1 cut(s) 988
AsuHPI GGTGA 1 cut(s) 1098
BamHI GGATCC 1 cut(s) 16
BanII GRGCYC 1 cut(s) 766
BbsI GAAGAC 1 cut(s) 116
Bbv12I GWGCWC 1 cut(s) 766
BbvI GCAGC 2 cut(s) 895, 1072
BccI CCATC 3 cut(s) 92, 905, 1111
BciT130I CCWGG 3 cut(s) 75, 338, 1110
BclI TGATCA 1 cut(s) 169
BfaI CTAG 3 cut(s) 242, 725, 888
BfrI CTTAAG 1 cut(s) 223
BisI GCNGC 2 cut(s) 884, 1086
BlsI GCNGC 2 cut(s) 885, 1087
BmcAI AGTACT 1 cut(s) 179
Bme1390I CCNGG 3 cut(s) 75, 338, 1110
BmiI GGNNCC 1 cut(s) 18
BmrFI CCNGG 3 cut(s) 75, 338, 1110
BmsI GCATC 1 cut(s) 366
BpiI GAAGAC 1 cut(s) 116
BplI GAGNNNNNCTC 2 cut(s) 69, 101
BpmI CTGGAG 1 cut(s) 96
BsaBI GATNNNNATC 2 cut(s) 405, 654
BsaJI CCNNGG 2 cut(s) 337, 829
Bsc4I CCNNNNNNNGG 1 cut(s) 1109
Bse1I ACTGG 1 cut(s) 262
Bse8I GATNNNNATC 2 cut(s) 405, 654
BseBI CCWGG 3 cut(s) 75, 338, 1110
BseDI CCNNGG 2 cut(s) 337, 829
BseGI GGATG 2 cut(s) 357, 433
BseJI GATNNNNATC 2 cut(s) 405, 654
BseLI CCNNNNNNNGG 1 cut(s) 1109
BseMII CTCAG 2 cut(s) 596, 693
BseNI ACTGG 1 cut(s) 262
BseRI GAGGAG 1 cut(s) 471
BseXI GCAGC 2 cut(s) 895, 1072
BseYI CCCAGC 1 cut(s) 968
Bsh1236I CGCG 2 cut(s) 988, 1064
BshFI GGCC 2 cut(s) 908, 925
BsiHKAI GWGCWC 1 cut(s) 766
BslI CCNNNNNNNGG 1 cut(s) 1109
BsnI GGCC 2 cut(s) 908, 925
Bsp1286I GDGCHC 1 cut(s) 766
Bsp143I GATC 6 cut(s) 16, 46, 169, 341, 406, 655
Bsp68I TCGCGA 1 cut(s) 1064
BspACI CCGC 2 cut(s) 629, 1072
BspANI GGCC 2 cut(s) 908, 925
BspCNI CTCAG 2 cut(s) 597, 694
BspFNI CGCG 2 cut(s) 988, 1064
BspLI GGNNCC 1 cut(s) 18
BspPI GGATC 6 cut(s) 11, 24, 54, 349, 401, 663
BspQI GCTCTTC 2 cut(s) 679, 759
BspTI CTTAAG 1 cut(s) 223
BsrI ACTGG 1 cut(s) 262
BssECI CCNNGG 2 cut(s) 337, 829
BssMI GATC 6 cut(s) 16, 46, 169, 341, 406, 655
Bst2UI CCWGG 3 cut(s) 75, 338, 1110
Bst4CI ACNGT 1 cut(s) 117
Bst6I CTCTTC 2 cut(s) 679, 759
BstAFI CTTAAG 1 cut(s) 223
BstC8I GCNNGC 1 cut(s) 816
BstDEI CTNAG 4 cut(s) 605, 702, 711, 875
BstDSI CCRYGG 1 cut(s) 829
BstF5I GGATG 2 cut(s) 357, 433
BstFNI CGCG 2 cut(s) 988, 1064
BstHHI GCGC 1 cut(s) 988
BstKTI GATC 6 cut(s) 19, 49, 172, 344, 409, 658
BstMBI GATC 6 cut(s) 16, 46, 169, 341, 406, 655
BstMWI GCNNNNNNNGC 2 cut(s) 811, 824
BstNI CCWGG 3 cut(s) 75, 338, 1110
BstSCI CCNGG 3 cut(s) 73, 336, 1108
BstUI CGCG 2 cut(s) 988, 1064
BstV1I GCAGC 2 cut(s) 895, 1072
BstV2I GAAGAC 1 cut(s) 116
BstX2I RGATCY 1 cut(s) 16
BstXI CCANNNNNNTGG 1 cut(s) 964
BstYI RGATCY 1 cut(s) 16
BsuRI GGCC 2 cut(s) 908, 925
BtgI CCRYGG 1 cut(s) 829
BtsCI GGATG 2 cut(s) 357, 433
BtsI GCAGTG 1 cut(s) 969
BtsIMutI CAGTG 1 cut(s) 969
BtuMI TCGCGA 1 cut(s) 1064
Cac8I GCNNGC 1 cut(s) 816
CaiI CAGNNNCTG 2 cut(s) 968, 1085
CfoI GCGC 1 cut(s) 988
CsiI ACCWGGT 1 cut(s) 1108
Csp6I GTAC 1 cut(s) 178
CspCI CAANNNNNGTGG 2 cut(s) 352, 387
CviAII CATG 3 cut(s) 329, 542, 811
CviQI GTAC 1 cut(s) 178
DdeI CTNAG 4 cut(s) 605, 702, 711, 875
DpnI GATC 6 cut(s) 18, 48, 171, 343, 408, 657
DpnII GATC 6 cut(s) 16, 46, 169, 341, 406, 655
DraI TTTAAA 1 cut(s) 775
DraIII CACNNNGTG 1 cut(s) 1112
DrdI GACNNNNNNGTC 1 cut(s) 275
DseDI GACNNNNNNGTC 1 cut(s) 275
EaeI YGGCCR 1 cut(s) 906
Eam1104I CTCTTC 2 cut(s) 679, 759
EarI CTCTTC 2 cut(s) 679, 759
Ecl136II GAGCTC 1 cut(s) 764
Eco24I GRGCYC 1 cut(s) 766
Eco32I GATATC 2 cut(s) 474, 872
Eco53kI GAGCTC 1 cut(s) 764
Eco57I CTGAAG 1 cut(s) 740
EcoICRI GAGCTC 1 cut(s) 764
EcoRII CCWGG 3 cut(s) 73, 336, 1108
EcoRV GATATC 2 cut(s) 474, 872
EcoT38I GRGCYC 1 cut(s) 766
FaeI CATG 3 cut(s) 332, 545, 814
FalI AAGNNNNNCTT 3 cut(s) 36, 658, 690
FatI CATG 3 cut(s) 328, 541, 810
FbaI TGATCA 1 cut(s) 169
FblI GTMKAC 2 cut(s) 279, 977
Fnu4HI GCNGC 2 cut(s) 884, 1086
FokI GGATG 2 cut(s) 344, 440
FriOI GRGCYC 1 cut(s) 766
Fsp4HI GCNGC 2 cut(s) 884, 1086
FspBI CTAG 3 cut(s) 242, 725, 888
GlaI GCGC 1 cut(s) 987
GluI GCNGC 2 cut(s) 884, 1086
GsaI CCCAGC 1 cut(s) 972
GsuI CTGGAG 1 cut(s) 96
HaeIII GGCC 2 cut(s) 908, 925
HhaI GCGC 1 cut(s) 988
Hin1II CATG 3 cut(s) 332, 545, 814
Hin6I GCGC 1 cut(s) 986
HinP1I GCGC 1 cut(s) 986
HincII GTYRAC 2 cut(s) 280, 1032
HindII GTYRAC 2 cut(s) 280, 1032
HinfI GANTC 3 cut(s) 34, 721, 990
HphI GGTGA 1 cut(s) 1098
Hpy166II GTNNAC 3 cut(s) 280, 978, 1032
Hpy188I TCNGA 5 cut(s) 268, 471, 606, 695, 759
Hpy188III TCNNGA 5 cut(s) 31, 515, 678, 767, 1063
Hpy8I GTNNAC 3 cut(s) 280, 978, 1032
HpyAV CCTTC 4 cut(s) 6, 30, 343, 997
HpyCH4III ACNGT 1 cut(s) 117
HpyCH4IV ACGT 1 cut(s) 894
HpyCH4V TGCA 3 cut(s) 437, 1088, 1095
HpyF10VI GCNNNNNNNGC 2 cut(s) 811, 824
HpyF3I CTNAG 4 cut(s) 605, 702, 711, 875
HpySE526I ACGT 1 cut(s) 894
Hsp92II CATG 3 cut(s) 332, 545, 814
HspAI GCGC 1 cut(s) 986
Ksp22I TGATCA 1 cut(s) 169
Kzo9I GATC 6 cut(s) 16, 46, 169, 341, 406, 655
LguI GCTCTTC 2 cut(s) 679, 759
LmnI GCTCC 1 cut(s) 77
Lsp1109I GCAGC 2 cut(s) 895, 1072
LweI GCATC 1 cut(s) 366
MabI ACCWGGT 1 cut(s) 1108
MaeI CTAG 3 cut(s) 242, 725, 888
MaeII ACGT 1 cut(s) 894
MaeIII GTNAC 3 cut(s) 836, 890, 895
MalI GATC 6 cut(s) 18, 48, 171, 343, 408, 657
MboI GATC 6 cut(s) 16, 46, 169, 341, 406, 655
MflI RGATCY 1 cut(s) 16
MhlI GDGCHC 1 cut(s) 766
MluCI AATT 8 cut(s) 69, 203, 237, 262, 844, 998, 1046, 1090
MlyI GAGTC 1 cut(s) 999
MmeI TCCRAC 2 cut(s) 176, 718
MnlI CCTC 6 cut(s) 449, 492, 498, 568, 646, 829
MroXI GAANNNNTTC 1 cut(s) 117
MseI TTAA 3 cut(s) 224, 231, 774
MslI CAYNNNNRTG 1 cut(s) 96
MspCI CTTAAG 1 cut(s) 223
MspR9I CCNGG 3 cut(s) 75, 338, 1110
MvaI CCWGG 3 cut(s) 75, 338, 1110
MvnI CGCG 2 cut(s) 988, 1064
MwoI GCNNNNNNNGC 2 cut(s) 811, 824
NdeII GATC 6 cut(s) 16, 46, 169, 341, 406, 655
NlaIII CATG 3 cut(s) 332, 545, 814
NlaIV GGNNCC 1 cut(s) 18
NmuCI GTSAC 1 cut(s) 895
NruI TCGCGA 1 cut(s) 1064
PciSI GCTCTTC 2 cut(s) 679, 759
PdmI GAANNNNTTC 1 cut(s) 117
PfeI GAWTC 2 cut(s) 34, 721
PfoI TCCNGGA 1 cut(s) 73
PkrI GCNGC 2 cut(s) 885, 1087
PleI GAGTC 1 cut(s) 998
PpsI GAGTC 1 cut(s) 998
PsiI TTATAA 1 cut(s) 552
Psp124BI GAGCTC 1 cut(s) 766
Psp6I CCWGG 3 cut(s) 73, 336, 1108
PspFI CCCAGC 1 cut(s) 968
PspGI CCWGG 3 cut(s) 73, 336, 1108
PspN4I GGNNCC 1 cut(s) 18
PstNI CAGNNNCTG 2 cut(s) 968, 1085
PsuI RGATCY 1 cut(s) 16
RruI TCGCGA 1 cut(s) 1064
RsaI GTAC 1 cut(s) 179
RsaNI GTAC 1 cut(s) 178
RseI CAYNNNNRTG 1 cut(s) 96
SacI GAGCTC 1 cut(s) 766
SalI GTCGAC 1 cut(s) 278
SapI GCTCTTC 2 cut(s) 679, 759
SaqAI TTAA 3 cut(s) 224, 231, 774
SatI GCNGC 2 cut(s) 884, 1086
Sau3AI GATC 6 cut(s) 16, 46, 169, 341, 406, 655
ScaI AGTACT 1 cut(s) 179
SchI GAGTC 1 cut(s) 999
ScrFI CCNGG 3 cut(s) 75, 338, 1110
SduI GDGCHC 1 cut(s) 766
SexAI ACCWGGT 1 cut(s) 1108
SfaNI GCATC 1 cut(s) 366
SmiMI CAYNNNNRTG 1 cut(s) 96
SmlI CTYRAG 1 cut(s) 223
SmoI CTYRAG 1 cut(s) 223
Sse9I AATT 8 cut(s) 69, 203, 237, 262, 844, 998, 1046, 1090
SsiI CCGC 2 cut(s) 629, 1072
SspI AATATT 1 cut(s) 423
SspMI CTAG 3 cut(s) 242, 725, 888
SstI GAGCTC 1 cut(s) 766
StyD4I CCNGG 3 cut(s) 73, 336, 1108
TaaI ACNGT 1 cut(s) 117
TaiI ACGT 1 cut(s) 897
TaqI TCGA 1 cut(s) 279
TasI AATT 8 cut(s) 69, 203, 237, 262, 844, 998, 1046, 1090
TatI WGTACW 1 cut(s) 177
TfiI GAWTC 2 cut(s) 34, 721
Tru1I TTAA 3 cut(s) 224, 231, 774
Tru9I TTAA 3 cut(s) 224, 231, 774
TscAI CASTG 1 cut(s) 969
TseFI GTSAC 1 cut(s) 895
TseI GCWGC 2 cut(s) 883, 1085
Tsp45I GTSAC 1 cut(s) 895
TspGWI ACGGA 1 cut(s) 399
TspRI CASTG 1 cut(s) 969
Vha464I CTTAAG 1 cut(s) 223
XapI RAATTY 5 cut(s) 203, 237, 262, 844, 1046
XcmI CCANNNNNNNNNTGG 1 cut(s) 141
XmiI GTMKAC 2 cut(s) 279, 977
XmnI GAANNNNTTC 1 cut(s) 117
XspI CTAG 3 cut(s) 242, 725, 888
ZrmI AGTACT 1 cut(s) 179
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.