FvH4_5g14275

B3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0000593
Physical Location & Seq
Reverse (-)
8077429 .. 8081550
4122 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g14275.t5

Sequence Viewer

Length: 1563 bp
ATGGCAACCAGAGTAAAGCTACCCTCTTTTCTCAAAATTGTTCCTGGTCATCAGTTCACTAACAAGCTGAAAATCCCAACAGAATTTCTCCAGCATTTCCATGGAAACGTGCCTCACCAGTGCCAACTTCAAACCGCAGCCCGAACTTGGCTTGTTCATGTGGAAGTGGTCAATAACAAGTTCTATTTCCAGGAGGGGTGGAATGCATTTCTGCGACAGAATGGCTTAAAATACGGGCAACTTCTAGTCTTCCATTATGCTGGAAACTCACACTTCCATGTGGATATCTTTGGAAAAAATACATGTGGAGAGTTGTTTGTAATGGCTGAAACAGAAGAAGCTGATGAAGAAGATTATGATGTATCTCTTGAGATCACAGACGGCTCTCCAGTTTCAAGTCCTCTGCCTCGAAAGAAGTACAAAACAAGTTCGAGTGTGAAAGAAAGAGGAGGCACCTCCAGCTCACAAAGATTCATAGAAAAGGATCTGTGGGGAGCTCGCATCAGAGCTGAAGAATTCAAATTAAAAAACCCTTCTGCTTTCTTGGTTTCCATGAAGCCCTCCTACATCAAGTATAAATTTGTGTGGTTGCCAGCAGAGTTCACCATGCTAGTGAAGCCTCGTATTGAGAACTCTGGTGATGTTATTCTTCGGGTTTCAGATTGGAGTGTGAGAACTTGGCGGGCCAAATTGACTTGTGATAATGTTTCTACCAACAGATGCAAATTACATGGTAGTTGGTCTGAATTTATGCGAGATAATAATTTGAAAGAAGGTGATCTGTGTGCATTTATGTTGATTAAAGACATCAGAGTTATAATCAATGTTGTCATTTTTCACACAAAAGAAGCAGCAAATCGCACCTTATCACCAGACATTTCTGATAATGAGGATCATGAGAATGATGAGGAGGGTGATGATGAAAGTGAAGATGAAGAAGAAACTGAAGATGAGGAGGAGGGAGATGATGCTTCTAGTGAAGCACTTAACTATGTTCCACCAGGCTCAAGAACAATAGCTTCGAGGCTTCACAAGAGAAGGAAAAACTGGACAAGTTCTATTGTTAAAGCACCACGCTTGTCTAGAGACGAAAAGATCCTAAATGCTATAGCTCTAAAAAAAGCTAATGCTTTCCAATCTGAGTCTACCCACCATTCTTTTGTGGTTTCCTTGTGTGCCTCATATGCCGGTGGAAGTTATCTGAGTTTTCCACGCACTTTTGCTGAGAGACATATTATTAATGGGCGCCCTAATTATGTCATCCTTCGGGTTAATTGGAGCTTTCTTGTCAATTCTAAAAAAAAAAGGAGCTTTCTTGTCAGGGTCAATTATATTGAGAGGACAACCAGATTACAAGCTGGTTGGTCTGAATTTGCGTATGTCAATAATCTGAAAATGGGCGATGTTTGTGTCTTTGAGTTGACCAATACCAAAAAGTTTGAATTTGAAGTTGAAATTTTCCGCATGACAGATGCTGTCTGCACTTCATCACAGGGGAAAGGAAAACAGCTGAGGACTGAGTCGATCAACTCAAGAATTAAGACAGAAATCCAGATAGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

521

Amino Acids

59.7

Weight (kDa)

7.63

Isoelectric Point (pI)

45.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 10 - 99 1e-11 B3 DNA binding domain
B3 PF02362 356 - 455 4.1e-13 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 818
AccB1I GGYRCC 2 cut(s) 452, 1245
AccI GTMKAC 1 cut(s) 1145
AciI CCGC 3 cut(s) 135, 682, 1462
AclWI GGATC 3 cut(s) 492, 900, 1090
AcsI RAATTY 7 cut(s) 83, 515, 578, 746, 1370, 1442, 1455
AcuI CTGAAG 2 cut(s) 531, 966
AcyI GRCGYC 1 cut(s) 1246
AfaI GTAC 1 cut(s) 419
AfiI CCNNNNNNNGG 1 cut(s) 147
AflIII ACRYGT 1 cut(s) 302
AgsI TTSAA 7 cut(s) 131, 396, 520, 769, 1442, 1448, 1454
AjnI CCWGG 3 cut(s) 43, 189, 1000
Alw21I GWGCWC 1 cut(s) 499
Alw26I GTCTC 2 cut(s) 1080, 1222
AlwI GGATC 3 cut(s) 492, 900, 1090
AlwNI CAGNNNCTG 1 cut(s) 1475
AoxI GGCC 1 cut(s) 684
ApeKI GCWGC 2 cut(s) 137, 851
ApoI RAATTY 7 cut(s) 83, 515, 578, 746, 1370, 1442, 1455
AseI ATTAAT 1 cut(s) 1239
AspLEI GCGC 1 cut(s) 1248
AspS9I GGNCC 1 cut(s) 684
AsuHPI GGTGA 6 cut(s) 107, 595, 650, 788, 861, 926
BanI GGYRCC 2 cut(s) 452, 1245
BanII GRGCYC 1 cut(s) 499
BarI GAAGNNNNNNTAC 2 cut(s) 548, 580
BbsI GAAGAC 1 cut(s) 241
Bbv12I GWGCWC 1 cut(s) 499
BbvCI CCTCAGC 1 cut(s) 1511
BbvI GCAGC 2 cut(s) 149, 863
BceAI ACGGC 1 cut(s) 397
BciT130I CCWGG 3 cut(s) 45, 191, 1002
BcoDI GTCTC 2 cut(s) 1080, 1222
BfaI CTAG 4 cut(s) 245, 611, 975, 1083
BfmI CTRYAG 1 cut(s) 1107
BfoI RGCGCY 1 cut(s) 1249
BisI GCNGC 2 cut(s) 138, 852
BlsI GCNGC 2 cut(s) 139, 853
Bme1390I CCNGG 3 cut(s) 45, 191, 1002
BmgT120I GGNCC 1 cut(s) 684
BmiI GGNNCC 2 cut(s) 454, 1247
BmrFI CCNGG 3 cut(s) 45, 191, 1002
BmsI GCATC 4 cut(s) 510, 710, 958, 1462
BpiI GAAGAC 1 cut(s) 241
BpmI CTGGAG 3 cut(s) 74, 372, 442
Bpu10I CCTNAGC 1 cut(s) 1511
BpuEI CTTGAG 3 cut(s) 389, 991, 1516
BsaHI GRCGYC 1 cut(s) 1246
BsaJI CCNNGG 1 cut(s) 100
Bsc4I CCNNNNNNNGG 1 cut(s) 147
Bse118I RCCGGY 1 cut(s) 1187
Bse1I ACTGG 3 cut(s) 118, 389, 1052
BseBI CCWGG 3 cut(s) 45, 191, 1002
BseDI CCNNGG 1 cut(s) 100
BseGI GGATG 1 cut(s) 1260
BseLI CCNNNNNNNGG 1 cut(s) 147
BseMII CTCAG 5 cut(s) 1131, 1193, 1215, 1502, 1509
BseNI ACTGG 3 cut(s) 118, 389, 1052
BseRI GAGGAG 4 cut(s) 462, 923, 968, 971
BseXI GCAGC 2 cut(s) 149, 863
BsgI GTGCAG 1 cut(s) 1465
BshFI GGCC 1 cut(s) 686
BshNI GGYRCC 2 cut(s) 452, 1245
BsiHKAI GWGCWC 1 cut(s) 499
BsiSI CCGG 1 cut(s) 1188
BslI CCNNNNNNNGG 1 cut(s) 147
BsmAI GTCTC 2 cut(s) 1080, 1222
BsmBI CGTCTC 1 cut(s) 1080
BsmI GAATGC 1 cut(s) 208
BsnI GGCC 1 cut(s) 686
Bsp1286I GDGCHC 1 cut(s) 499
Bsp143I GATC 6 cut(s) 372, 484, 778, 892, 1095, 1524
Bsp19I CCATGG 1 cut(s) 100
BspACI CCGC 3 cut(s) 135, 682, 1462
BspANI GGCC 1 cut(s) 686
BspCNI CTCAG 5 cut(s) 1132, 1194, 1216, 1503, 1510
BspHI TCATGA 1 cut(s) 895
BspLI GGNNCC 2 cut(s) 454, 1247
BspPI GGATC 3 cut(s) 492, 900, 1090
BspT107I GGYRCC 2 cut(s) 452, 1245
BsrFI RCCGGY 1 cut(s) 1187
BsrI ACTGG 3 cut(s) 118, 389, 1052
BssAI RCCGGY 1 cut(s) 1187
BssECI CCNNGG 1 cut(s) 100
BssMI GATC 6 cut(s) 372, 484, 778, 892, 1095, 1524
BssNI GRCGYC 1 cut(s) 1246
BssT1I CCWWGG 1 cut(s) 100
Bst2UI CCWGG 3 cut(s) 45, 191, 1002
BstACI GRCGYC 1 cut(s) 1246
BstC8I GCNNGC 3 cut(s) 499, 594, 684
BstDEI CTNAG 5 cut(s) 1140, 1202, 1224, 1511, 1518
BstDSI CCRYGG 1 cut(s) 100
BstF5I GGATG 1 cut(s) 1260
BstH2I RGCGCY 1 cut(s) 1249
BstHHI GCGC 1 cut(s) 1248
BstKTI GATC 6 cut(s) 375, 487, 781, 895, 1098, 1527
BstMAI GTCTC 2 cut(s) 1080, 1222
BstMBI GATC 6 cut(s) 372, 484, 778, 892, 1095, 1524
BstMWI GCNNNNNNNGC 3 cut(s) 459, 616, 1184
BstNI CCWGG 3 cut(s) 45, 191, 1002
BstNSI RCATGY 1 cut(s) 306
BstSCI CCNGG 3 cut(s) 43, 189, 1000
BstSFI CTRYAG 1 cut(s) 1107
BstV1I GCAGC 2 cut(s) 149, 863
BstV2I GAAGAC 1 cut(s) 241
BstX2I RGATCY 2 cut(s) 484, 1095
BstXI CCANNNNNNTGG 1 cut(s) 260
BstYI RGATCY 2 cut(s) 484, 1095
BsuRI GGCC 1 cut(s) 686
BtgI CCRYGG 1 cut(s) 100
BtgZI GCGATG 1 cut(s) 1416
BtsCI GGATG 1 cut(s) 1260
BtsIMutI CAGTG 1 cut(s) 125
Cac8I GCNNGC 3 cut(s) 499, 594, 684
CaiI CAGNNNCTG 1 cut(s) 1475
CciI TCATGA 1 cut(s) 895
CfoI GCGC 1 cut(s) 1248
Cfr10I RCCGGY 1 cut(s) 1187
Cfr13I GGNCC 1 cut(s) 684
Csp6I GTAC 1 cut(s) 418
CviAII CATG 9 cut(s) 101, 158, 278, 303, 553, 607, 731, 896, 1465
CviQI GTAC 1 cut(s) 418
DdeI CTNAG 5 cut(s) 1140, 1202, 1224, 1511, 1518
DinI GGCGCC 1 cut(s) 1247
DpnI GATC 6 cut(s) 374, 486, 780, 894, 1097, 1526
DpnII GATC 6 cut(s) 372, 484, 778, 892, 1095, 1524
Ecl136II GAGCTC 1 cut(s) 497
Eco130I CCWWGG 1 cut(s) 100
Eco24I GRGCYC 1 cut(s) 499
Eco32I GATATC 1 cut(s) 286
Eco53kI GAGCTC 1 cut(s) 497
Eco57I CTGAAG 2 cut(s) 531, 966
EcoICRI GAGCTC 1 cut(s) 497
EcoRI GAATTC 1 cut(s) 515
EcoRII CCWGG 3 cut(s) 43, 189, 1000
EcoRV GATATC 1 cut(s) 286
EcoT14I CCWWGG 1 cut(s) 100
EcoT22I ATGCAT 1 cut(s) 208
EcoT38I GRGCYC 1 cut(s) 499
EgeI GGCGCC 1 cut(s) 1247
EheI GGCGCC 1 cut(s) 1247
ErhI CCWWGG 1 cut(s) 100
Esp3I CGTCTC 1 cut(s) 1080
FaeI CATG 9 cut(s) 104, 161, 281, 306, 556, 610, 734, 899, 1468
FatI CATG 9 cut(s) 100, 157, 277, 302, 552, 606, 730, 895, 1464
FauI CCCGC 1 cut(s) 675
FauNDI CATATG 1 cut(s) 1183
FblI GTMKAC 1 cut(s) 1145
Fnu4HI GCNGC 2 cut(s) 138, 852
FokI GGATG 1 cut(s) 1247
FriOI GRGCYC 1 cut(s) 499
Fsp4HI GCNGC 2 cut(s) 138, 852
FspBI CTAG 4 cut(s) 245, 611, 975, 1083
GlaI GCGC 1 cut(s) 1247
GluI GCNGC 2 cut(s) 138, 852
GsuI CTGGAG 3 cut(s) 74, 372, 442
HaeII RGCGCY 1 cut(s) 1249
HaeIII GGCC 1 cut(s) 686
HapII CCGG 1 cut(s) 1188
HhaI GCGC 1 cut(s) 1248
Hin1I GRCGYC 1 cut(s) 1246
Hin1II CATG 9 cut(s) 104, 161, 281, 306, 556, 610, 734, 899, 1468
Hin6I GCGC 1 cut(s) 1246
HinP1I GCGC 1 cut(s) 1246
HincII GTYRAC 1 cut(s) 1422
HindII GTYRAC 1 cut(s) 1422
HinfI GANTC 3 cut(s) 471, 1142, 1520
HpaII CCGG 1 cut(s) 1188
HphI GGTGA 6 cut(s) 107, 595, 650, 788, 861, 926
Hpy166II GTNNAC 4 cut(s) 57, 603, 1146, 1422
Hpy188I TCNGA 9 cut(s) 506, 661, 745, 812, 883, 1141, 1203, 1369, 1392
Hpy188III TCNNGA 6 cut(s) 368, 896, 1008, 1083, 1533, 1552
Hpy8I GTNNAC 4 cut(s) 57, 603, 1146, 1422
HpyAV CCTTC 4 cut(s) 543, 767, 1032, 1274
HpyCH4IV ACGT 1 cut(s) 108
HpyCH4V TGCA 4 cut(s) 206, 723, 788, 1482
HpyF10VI GCNNNNNNNGC 3 cut(s) 459, 616, 1184
HpyF3I CTNAG 5 cut(s) 1140, 1202, 1224, 1511, 1518
HpySE526I ACGT 1 cut(s) 108
Hsp92I GRCGYC 1 cut(s) 1246
Hsp92II CATG 9 cut(s) 104, 161, 281, 306, 556, 610, 734, 899, 1468
HspAI GCGC 1 cut(s) 1246
KasI GGCGCC 1 cut(s) 1245
Kzo9I GATC 6 cut(s) 372, 484, 778, 892, 1095, 1524
LmnI GCTCC 3 cut(s) 494, 1278, 1308
Lsp1109I GCAGC 2 cut(s) 149, 863
LweI GCATC 4 cut(s) 510, 710, 958, 1462
MaeI CTAG 4 cut(s) 245, 611, 975, 1083
MaeII ACGT 1 cut(s) 108
MalI GATC 6 cut(s) 374, 486, 780, 894, 1097, 1526
MboI GATC 6 cut(s) 372, 484, 778, 892, 1095, 1524
MflI RGATCY 2 cut(s) 484, 1095
MhlI GDGCHC 1 cut(s) 499
Mly113I GGCGCC 1 cut(s) 1246
MlyI GAGTC 2 cut(s) 1151, 1529
Mph1103I ATGCAT 1 cut(s) 208
MseI TTAA 8 cut(s) 227, 524, 801, 987, 1065, 1239, 1272, 1539
MslI CAYNNNNRTG 4 cut(s) 99, 276, 611, 900
MspA1I CMGCKG 1 cut(s) 1510
MspI CCGG 1 cut(s) 1188
MspR9I CCNGG 3 cut(s) 45, 191, 1002
Mva1269I GAATGC 1 cut(s) 208
MvaI CCWGG 3 cut(s) 45, 191, 1002
MwoI GCNNNNNNNGC 3 cut(s) 459, 616, 1184
NarI GGCGCC 1 cut(s) 1246
NcoI CCATGG 1 cut(s) 100
NdeI CATATG 1 cut(s) 1183
NdeII GATC 6 cut(s) 372, 484, 778, 892, 1095, 1524
NlaIII CATG 9 cut(s) 104, 161, 281, 306, 556, 610, 734, 899, 1468
NlaIV GGNNCC 2 cut(s) 454, 1247
NsiI ATGCAT 1 cut(s) 208
NspI RCATGY 1 cut(s) 306
PagI TCATGA 1 cut(s) 895
PciI ACATGT 1 cut(s) 302
PctI GAATGC 1 cut(s) 208
PfeI GAWTC 1 cut(s) 471
PflFI GACNNNGTC 1 cut(s) 1519
PfoI TCCNGGA 1 cut(s) 189
PkrI GCNGC 2 cut(s) 139, 853
PleI GAGTC 2 cut(s) 1150, 1528
PluTI GGCGCC 1 cut(s) 1249
PpsI GAGTC 2 cut(s) 1150, 1528
PscI ACATGT 1 cut(s) 302
PshBI ATTAAT 1 cut(s) 1239
PsiI TTATAA 1 cut(s) 818
Psp124BI GAGCTC 1 cut(s) 499
Psp6I CCWGG 3 cut(s) 43, 189, 1000
PspGI CCWGG 3 cut(s) 43, 189, 1000
PspN4I GGNNCC 2 cut(s) 454, 1247
PspPI GGNCC 1 cut(s) 684
PstNI CAGNNNCTG 1 cut(s) 1475
PsuI RGATCY 2 cut(s) 484, 1095
PsyI GACNNNGTC 1 cut(s) 1519
PvuII CAGCTG 1 cut(s) 1510
RsaI GTAC 1 cut(s) 419
RsaNI GTAC 1 cut(s) 418
RseI CAYNNNNRTG 4 cut(s) 99, 276, 611, 900
SacI GAGCTC 1 cut(s) 499
SaqAI TTAA 8 cut(s) 227, 524, 801, 987, 1065, 1239, 1272, 1539
SatI GCNGC 2 cut(s) 138, 852
Sau3AI GATC 6 cut(s) 372, 484, 778, 892, 1095, 1524
Sau96I GGNCC 1 cut(s) 684
SchI GAGTC 2 cut(s) 1151, 1529
ScrFI CCNGG 3 cut(s) 45, 191, 1002
SduI GDGCHC 1 cut(s) 499
SfaNI GCATC 4 cut(s) 510, 710, 958, 1462
SfcI CTRYAG 1 cut(s) 1107
SfoI GGCGCC 1 cut(s) 1247
SmiMI CAYNNNNRTG 4 cut(s) 99, 276, 611, 900
SmlI CTYRAG 3 cut(s) 368, 1006, 1531
SmoI CTYRAG 3 cut(s) 368, 1006, 1531
SsiI CCGC 3 cut(s) 135, 682, 1462
SspDI GGCGCC 1 cut(s) 1245
SspMI CTAG 4 cut(s) 245, 611, 975, 1083
SstI GAGCTC 1 cut(s) 499
StyD4I CCNGG 3 cut(s) 43, 189, 1000
StyI CCWWGG 1 cut(s) 100
TaiI ACGT 1 cut(s) 111
TaqI TCGA 4 cut(s) 409, 431, 1022, 1523
TatI WGTACW 1 cut(s) 417
TfiI GAWTC 1 cut(s) 471
Tru1I TTAA 8 cut(s) 227, 524, 801, 987, 1065, 1239, 1272, 1539
Tru9I TTAA 8 cut(s) 227, 524, 801, 987, 1065, 1239, 1272, 1539
TscAI CASTG 1 cut(s) 125
TseI GCWGC 2 cut(s) 137, 851
TspDTI ATGAA 7 cut(s) 146, 360, 463, 569, 936, 948, 1476
TspRI CASTG 1 cut(s) 125
Tth111I GACNNNGTC 1 cut(s) 1519
VspI ATTAAT 1 cut(s) 1239
XapI RAATTY 7 cut(s) 83, 515, 578, 746, 1370, 1442, 1455
XbaI TCTAGA 1 cut(s) 1082
XceI RCATGY 1 cut(s) 306
XcmI CCANNNNNNNNNTGG 1 cut(s) 98
XmiI GTMKAC 1 cut(s) 1145
XspI CTAG 4 cut(s) 245, 611, 975, 1083
Zsp2I ATGCAT 1 cut(s) 208
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.