Rh2AG162900

B3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0000593
Physical Location & Seq
Reverse (-)
15391905 .. 15394575
2671 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG162900.1

Sequence Viewer

Length: 1446 bp
ATGGCAACAACAAGAAAACCCTCTTTCTTCAAGGTTCTTATAGGGGATCACTTCTGTAACAGATTGAAAATTCCCGGAGCTTTTCTTGAGCATTTTGATGGGAGAGTACCTGAACAGTGCCATCTTAGAATCGGCACTCAGACTTGGCTTGTTGATGTTGAAATGGTCCATCAGAAGTTCTATTTCCAAAATGGTTGGAAGGAATTTGTGCTTGAGAATGGTTTAAGGGGTTGCGAGTTTCTAGTGTTTTTCTATAATGGAAATTCGGAGTTCTATGTCCACATATATGGGAAGAATGCTTGCAGAAAGGAGTTTGCAGATGCCGCTGCTGGAGTAAGTGGCACACCGAAATCAATGCATGTTGGTAGCTCCAGAGGGATGGAAGAAGCTGATAGTAATGACTCATCTGTTCAGATCCTGGATGACTTTCCAACCTGCTCAAGAAGAGCAGGGGAGAAATCTTCATTACCAAGTCATCAGCAGCACAAGAAAGACAGTACAAGCTGGATTGGTAAAGAGACACATGGTCCCAAACCAGGAATTACAAGAAAAGATAATGATTTTCCGGCGGAACAATATGTGGGAGGGACTTCTAGCTTGAAACCAAAACATGAGGTTCTTGGGAGCTGGATGCACACTGATGATGACGATGATGACAAAGAAGAGGAAGAGAGTGAGGAGGATGATGATGATTATGAGGAAGATGAAGAGAGTGAGGAGGATGAAAATGAAGCTGACAGTGAAGACAAAGATGGGTATGAAGATGAAGAAGAGGTAGAAGAAAGCGATGGAGATGGGGGAAAAAGTGATGATTCCATTGAAATTCTGGACGAGTTTCCACGATGCTCAAAAGCAAAAACAATGAGGAAATCATCAGGGCTTCAGAAGAGAAAGATAAACAGTTCAAAAGCTAAAACAAAAAGAAGCATAACACGTGCTAGTGGCTCGTCTAGCAATCAAAAAATCACGGGATGGAAATATAAGGTTTTAAGGAAAATGCCTGGATCGAATGGAATCGGAGAACTTATAGCCCGTCACAGAGCTAAAGATTTCATATCCAAATCTGACAAGCTGGATTCTTGTTTGATTGCCATGTCTCCTTCGTATATTAGAGGATCTTGTGTGCATTTTCCACGTACTTTTGCCTACAGACATCTTGGAAGGCATTGTAGTCGCATCAAACTTCGGGTTAATAAGAGGAGGTGGTGTCTTGGTATCTATCATGTCCATAAAACAGTTAGATTACAAGCTGGATGGATTGAATTTAAGCATGACAATAATCTGAAAGTGGGTGATGTGTGCGTCTTTGAATTGACTGACAGCATCAATGTACTTGATGTTACAATTTTCCGCGCCGCAGAAGCTGCAAATTGCACCTTGCCAGGGGGAAGACAAACGACTGGGGTTCAATTCAAGTCGGAAAAAGGAAAAGAGAAAGCTGGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

481

Amino Acids

54.35

Weight (kDa)

6.02

Isoelectric Point (pI)

52.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 9 - 98 8e-11 B3 DNA binding domain
B3 PF02362 366 - 452 3.5e-10 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 443
AccII CGCG 1 cut(s) 1353
AciI CCGC 4 cut(s) 324, 569, 1351, 1356
AclWI GGATC 4 cut(s) 54, 409, 1012, 1123
AcsI RAATTY 5 cut(s) 69, 203, 262, 822, 1262
AcuI CTGAAG 1 cut(s) 866
AcvI CACGTG 1 cut(s) 935
AfaI GTAC 4 cut(s) 108, 499, 1138, 1332
AfiI CCNNNNNNNGG 2 cut(s) 536, 1383
AflIII ACRYGT 1 cut(s) 932
AhdI GACNNNNNGTC 1 cut(s) 525
AjnI CCWGG 4 cut(s) 417, 535, 1000, 1381
Alw26I GTCTC 2 cut(s) 512, 1101
AlwI GGATC 4 cut(s) 54, 409, 1012, 1123
AlwNI CAGNNNCTG 2 cut(s) 418, 1364
ApeKI GCWGC 3 cut(s) 326, 481, 1364
ApoI RAATTY 5 cut(s) 69, 203, 262, 822, 1262
AspLEI GCGC 1 cut(s) 1355
AspS9I GGNCC 2 cut(s) 166, 527
AsuC2I CCSGG 1 cut(s) 75
AsuHPI GGTGA 1 cut(s) 1304
AvaII GGWCC 2 cut(s) 166, 527
BbrPI CACGTG 1 cut(s) 935
BbsI GAAGAC 2 cut(s) 750, 1396
BbvI GCAGC 3 cut(s) 313, 493, 1351
BccI CCATC 9 cut(s) 92, 129, 177, 373, 746, 782, 788, 966, 1248
BcgI CGANNNNNNTGC 4 cut(s) 337, 371, 1154, 1188
BciT130I CCWGG 4 cut(s) 419, 537, 1002, 1383
BcnI CCSGG 1 cut(s) 75
BcoDI GTCTC 2 cut(s) 512, 1101
BfaI CTAG 4 cut(s) 242, 594, 939, 951
BfmI CTRYAG 1 cut(s) 1147
BfuAI ACCTGC 1 cut(s) 443
BisI GCNGC 5 cut(s) 324, 327, 482, 1356, 1365
BlsI GCNGC 5 cut(s) 325, 328, 483, 1357, 1366
Bme1390I CCNGG 5 cut(s) 75, 419, 537, 1002, 1383
Bme18I GGWCC 2 cut(s) 166, 527
BmeRI GACNNNNNGTC 1 cut(s) 525
BmgT120I GGNCC 2 cut(s) 166, 527
BmiI GGNNCC 1 cut(s) 529
BmrFI CCNGG 5 cut(s) 75, 419, 537, 1002, 1383
BmrI ACTGGG 1 cut(s) 1410
BmsI GCATC 5 cut(s) 310, 621, 833, 1185, 1332
BmuI ACTGGG 1 cut(s) 1410
BpiI GAAGAC 2 cut(s) 750, 1396
BpmI CTGGAG 2 cut(s) 351, 355
BpuEI CTTGAG 3 cut(s) 107, 233, 424
BpuMI CCSGG 1 cut(s) 75
BsaAI YACGTR 2 cut(s) 935, 1136
BsaJI CCNNGG 1 cut(s) 1382
BsaXI ACNNNNNCTCC 2 cut(s) 1192, 1222
Bsc4I CCNNNNNNNGG 2 cut(s) 536, 1383
Bse1I ACTGG 1 cut(s) 1405
BseBI CCWGG 4 cut(s) 419, 537, 1002, 1383
BseDI CCNNGG 1 cut(s) 1382
BseGI GGATG 7 cut(s) 384, 427, 636, 688, 727, 977, 1259
BseLI CCNNNNNNNGG 2 cut(s) 536, 1383
BseMII CTCAG 1 cut(s) 152
BseNI ACTGG 1 cut(s) 1405
BseRI GAGGAG 3 cut(s) 692, 731, 1213
BseXI GCAGC 3 cut(s) 313, 493, 1351
Bsh1236I CGCG 1 cut(s) 1353
BsiSI CCGG 2 cut(s) 75, 566
BslFI GGGAC 2 cut(s) 513, 601
BslI CCNNNNNNNGG 2 cut(s) 536, 1383
BsmAI GTCTC 2 cut(s) 512, 1101
BsmFI GGGAC 2 cut(s) 513, 601
BsmI GAATGC 1 cut(s) 301
Bsp143I GATC 4 cut(s) 46, 414, 1004, 1115
BspACI CCGC 4 cut(s) 324, 569, 1351, 1356
BspCNI CTCAG 1 cut(s) 151
BspFNI CGCG 1 cut(s) 1353
BspLI GGNNCC 1 cut(s) 529
BspMI ACCTGC 1 cut(s) 443
BspPI GGATC 4 cut(s) 54, 409, 1012, 1123
BspQI GCTCTTC 1 cut(s) 439
BsrI ACTGG 1 cut(s) 1405
BssECI CCNNGG 1 cut(s) 1382
BssMI GATC 4 cut(s) 46, 414, 1004, 1115
Bst2UI CCWGG 4 cut(s) 419, 537, 1002, 1383
Bst4CI ACNGT 5 cut(s) 117, 497, 740, 902, 1237
Bst6I CTCTTC 6 cut(s) 439, 657, 663, 702, 765, 881
BstAPI GCANNNNNTGC 1 cut(s) 1364
BstBAI YACGTR 2 cut(s) 935, 1136
BstC8I GCNNGC 1 cut(s) 301
BstDEI CTNAG 2 cut(s) 125, 138
BstF5I GGATG 7 cut(s) 384, 427, 636, 688, 727, 977, 1259
BstFNI CGCG 1 cut(s) 1353
BstHHI GCGC 1 cut(s) 1355
BstKTI GATC 4 cut(s) 49, 417, 1007, 1118
BstMAI GTCTC 2 cut(s) 512, 1101
BstMBI GATC 4 cut(s) 46, 414, 1004, 1115
BstMWI GCNNNNNNNGC 4 cut(s) 323, 951, 1361, 1364
BstNI CCWGG 4 cut(s) 419, 537, 1002, 1383
BstNSI RCATGY 1 cut(s) 362
BstSCI CCNGG 5 cut(s) 73, 417, 535, 1000, 1381
BstSFI CTRYAG 1 cut(s) 1147
BstUI CGCG 1 cut(s) 1353
BstV1I GCAGC 3 cut(s) 313, 493, 1351
BstV2I GAAGAC 2 cut(s) 750, 1396
BstX2I RGATCY 2 cut(s) 414, 1115
BstXI CCANNNNNNTGG 2 cut(s) 287, 379
BstYI RGATCY 2 cut(s) 414, 1115
BtgZI GCGATG 1 cut(s) 801
BtsCI GGATG 7 cut(s) 384, 427, 636, 688, 727, 977, 1259
BtsIMutI CAGTG 3 cut(s) 122, 636, 745
BveI ACCTGC 1 cut(s) 443
Cac8I GCNNGC 1 cut(s) 301
CaiI CAGNNNCTG 2 cut(s) 418, 1364
CfoI GCGC 1 cut(s) 1355
Cfr13I GGNCC 2 cut(s) 166, 527
CseI GACGC 1 cut(s) 1291
Csp6I GTAC 4 cut(s) 107, 498, 1137, 1331
CviAII CATG 6 cut(s) 359, 524, 611, 1093, 1223, 1271
CviQI GTAC 4 cut(s) 107, 498, 1137, 1331
DdeI CTNAG 2 cut(s) 125, 138
DpnI GATC 4 cut(s) 48, 416, 1006, 1117
DpnII GATC 4 cut(s) 46, 414, 1004, 1115
DriI GACNNNNNGTC 1 cut(s) 525
Eam1104I CTCTTC 6 cut(s) 439, 657, 663, 702, 765, 881
Eam1105I GACNNNNNGTC 1 cut(s) 525
EarI CTCTTC 6 cut(s) 439, 657, 663, 702, 765, 881
EciI GGCGGA 1 cut(s) 584
Eco47I GGWCC 2 cut(s) 166, 527
Eco57I CTGAAG 1 cut(s) 866
Eco72I CACGTG 1 cut(s) 935
EcoRII CCWGG 4 cut(s) 417, 535, 1000, 1381
EcoT22I ATGCAT 1 cut(s) 360
FaeI CATG 6 cut(s) 362, 527, 614, 1096, 1226, 1274
FaqI GGGAC 2 cut(s) 513, 601
FatI CATG 6 cut(s) 358, 523, 610, 1092, 1222, 1270
Fnu4HI GCNGC 5 cut(s) 324, 327, 482, 1356, 1365
FokI GGATG 7 cut(s) 391, 434, 643, 695, 734, 984, 1266
Fsp4HI GCNGC 5 cut(s) 324, 327, 482, 1356, 1365
FspBI CTAG 4 cut(s) 242, 594, 939, 951
GlaI GCGC 1 cut(s) 1354
GluI GCNGC 5 cut(s) 324, 327, 482, 1356, 1365
GsuI CTGGAG 2 cut(s) 351, 355
HapII CCGG 2 cut(s) 75, 566
HgaI GACGC 1 cut(s) 1291
HhaI GCGC 1 cut(s) 1355
Hin1II CATG 6 cut(s) 362, 527, 614, 1096, 1226, 1274
Hin6I GCGC 1 cut(s) 1353
HinP1I GCGC 1 cut(s) 1353
HinfI GANTC 5 cut(s) 129, 401, 812, 1014, 1076
HpaII CCGG 2 cut(s) 75, 566
HphI GGTGA 1 cut(s) 1304
Hpy166II GTNNAC 1 cut(s) 280
Hpy188I TCNGA 9 cut(s) 141, 174, 268, 414, 885, 1019, 1066, 1284, 1420
Hpy188III TCNNGA 4 cut(s) 86, 372, 441, 827
Hpy8I GTNNAC 1 cut(s) 280
HpyAV CCTTC 3 cut(s) 193, 1110, 1155
HpyCH4III ACNGT 5 cut(s) 117, 497, 740, 902, 1237
HpyCH4IV ACGT 2 cut(s) 934, 1135
HpyCH4V TGCA 7 cut(s) 303, 317, 358, 634, 1126, 1367, 1374
HpyF10VI GCNNNNNNNGC 4 cut(s) 323, 951, 1361, 1364
HpyF3I CTNAG 2 cut(s) 125, 138
HpySE526I ACGT 2 cut(s) 934, 1135
Hsp92II CATG 6 cut(s) 362, 527, 614, 1096, 1226, 1274
HspAI GCGC 1 cut(s) 1353
Kzo9I GATC 4 cut(s) 46, 414, 1004, 1115
LguI GCTCTTC 1 cut(s) 439
LmnI GCTCC 3 cut(s) 77, 374, 624
Lsp1109I GCAGC 3 cut(s) 313, 493, 1351
LweI GCATC 5 cut(s) 310, 621, 833, 1185, 1332
MaeI CTAG 4 cut(s) 242, 594, 939, 951
MaeII ACGT 2 cut(s) 934, 1135
MaeIII GTNAC 3 cut(s) 56, 1034, 1339
MalI GATC 4 cut(s) 48, 416, 1006, 1117
MboI GATC 4 cut(s) 46, 414, 1004, 1115
MflI RGATCY 2 cut(s) 414, 1115
MlyI GAGTC 1 cut(s) 395
MmeI TCCRAC 3 cut(s) 176, 455, 1398
Mph1103I ATGCAT 1 cut(s) 360
MseI TTAA 4 cut(s) 224, 989, 1191, 1266
MslI CAYNNNNRTG 3 cut(s) 96, 285, 639
MspA1I CMGCKG 1 cut(s) 326
MspI CCGG 2 cut(s) 75, 566
MspR9I CCNGG 5 cut(s) 75, 419, 537, 1002, 1383
Mva1269I GAATGC 1 cut(s) 301
MvaI CCWGG 4 cut(s) 419, 537, 1002, 1383
MvnI CGCG 1 cut(s) 1353
MwoI GCNNNNNNNGC 4 cut(s) 323, 951, 1361, 1364
NciI CCSGG 1 cut(s) 75
NdeII GATC 4 cut(s) 46, 414, 1004, 1115
NlaIII CATG 6 cut(s) 362, 527, 614, 1096, 1226, 1274
NlaIV GGNNCC 1 cut(s) 529
NmuCI GTSAC 1 cut(s) 1034
NsiI ATGCAT 1 cut(s) 360
NspI RCATGY 1 cut(s) 362
PciSI GCTCTTC 1 cut(s) 439
PctI GAATGC 1 cut(s) 301
PfeI GAWTC 4 cut(s) 129, 812, 1014, 1076
PfoI TCCNGGA 2 cut(s) 73, 417
PkrI GCNGC 5 cut(s) 325, 328, 483, 1357, 1366
PleI GAGTC 1 cut(s) 395
PmaCI CACGTG 1 cut(s) 935
PmlI CACGTG 1 cut(s) 935
PpsI GAGTC 1 cut(s) 395
Ppu21I YACGTR 2 cut(s) 935, 1136
Psp6I CCWGG 4 cut(s) 417, 535, 1000, 1381
PspCI CACGTG 1 cut(s) 935
PspGI CCWGG 4 cut(s) 417, 535, 1000, 1381
PspN4I GGNNCC 1 cut(s) 529
PspPI GGNCC 2 cut(s) 166, 527
PstNI CAGNNNCTG 2 cut(s) 418, 1364
PsuI RGATCY 2 cut(s) 414, 1115
RsaI GTAC 4 cut(s) 108, 499, 1138, 1332
RsaNI GTAC 4 cut(s) 107, 498, 1137, 1331
RseI CAYNNNNRTG 3 cut(s) 96, 285, 639
SapI GCTCTTC 1 cut(s) 439
SaqAI TTAA 4 cut(s) 224, 989, 1191, 1266
SatI GCNGC 5 cut(s) 324, 327, 482, 1356, 1365
Sau3AI GATC 4 cut(s) 46, 414, 1004, 1115
Sau96I GGNCC 2 cut(s) 166, 527
SchI GAGTC 1 cut(s) 395
ScrFI CCNGG 5 cut(s) 75, 419, 537, 1002, 1383
SfaNI GCATC 5 cut(s) 310, 621, 833, 1185, 1332
SfcI CTRYAG 1 cut(s) 1147
SinI GGWCC 2 cut(s) 166, 527
SmiMI CAYNNNNRTG 3 cut(s) 96, 285, 639
SmlI CTYRAG 3 cut(s) 86, 212, 439
SmoI CTYRAG 3 cut(s) 86, 212, 439
SsiI CCGC 4 cut(s) 324, 569, 1351, 1356
SspMI CTAG 4 cut(s) 242, 594, 939, 951
StyD4I CCNGG 5 cut(s) 73, 417, 535, 1000, 1381
TaaI ACNGT 5 cut(s) 117, 497, 740, 902, 1237
TaiI ACGT 2 cut(s) 937, 1138
TaqI TCGA 1 cut(s) 1007
TatI WGTACW 2 cut(s) 497, 1330
TauI GCSGC 2 cut(s) 326, 1358
TfiI GAWTC 4 cut(s) 129, 812, 1014, 1076
Tru1I TTAA 4 cut(s) 224, 989, 1191, 1266
Tru9I TTAA 4 cut(s) 224, 989, 1191, 1266
TscAI CASTG 3 cut(s) 122, 643, 745
TseFI GTSAC 1 cut(s) 1034
TseI GCWGC 3 cut(s) 326, 481, 1364
Tsp45I GTSAC 1 cut(s) 1034
TspDTI ATGAA 7 cut(s) 453, 720, 738, 744, 774, 780, 1042
TspRI CASTG 3 cut(s) 122, 643, 745
VpaK11BI GGWCC 2 cut(s) 166, 527
XapI RAATTY 5 cut(s) 69, 203, 262, 822, 1262
XceI RCATGY 1 cut(s) 362
XcmI CCANNNNNNNNNTGG 1 cut(s) 823
XspI CTAG 4 cut(s) 242, 594, 939, 951
Zsp2I ATGCAT 1 cut(s) 360
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.