Rorug02G0220000

B3 DNA binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0000593
Physical Location & Seq
Forward (+)
21218227 .. 21220985
2759 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0220000.1

Sequence Viewer

Length: 921 bp
ATGGATAATCCGAGCAGCAGCGGCGTACCCGAGTCCGAAAAGCTTCTCGGAATCGCCGCCGATCTCCTCTGCGGCGGCGACTTCGCTTCTTGCCGGAAATTCGCTCTCTTCGCCCGCGACTGCGACCCGGAAAACCCCGTCGCGGAAAGAATCCTCGCCGTCGCCGACGTTCTCCTCGCCGAGAAGAGGCGCGACCCGACCGACTGGTACCCCATTTTGCAACTGACCCGACCCGACTCCGGGAACCGCGGCCTCGTTCGGACCCAGTTCGAGAAGCTGACGGCGCTGTTGAACCCGGAGGACAACGGCTTCCCGTTCTCCGACGTGGCGCTCGGGCTGGTCCGCAAGGCGTGGGCCGCGCTGTCCGACCCGGGGTTCGGAAATGGGCCCAAGAACGGGCCGGAAAGCCCGAAACTTGAAGAGGGTTTGGCCGGAGAGACGTTCTGGACGGTTTGTCCGTACTGCTATGGCATGTTTATGTACAAGAAGGTGTTTGAGGAGTGTTGCCTGAGGTGTCAGATTTGCCGGAAGGCGTTTCACGGCGTGGCGATTAGGCCGCCGGCGCCGGAGATTTTGGTGGAGGGGAAGGAGCAGTACTATTTTACTTATGGGTGTTTTCCTATGGAGTACAATGAGGAAGCAAAGAAGAGTAATGAGGACACAAAGAAGAGGCAGATGGAGGAGGGTGTTTGTGTTGTGGAGATTTCTGATGATGAGGAGGAGGTGGTGGTGAAGAATGTGGGTGGAGGAGGGAAGTTTTCGAGCGAAGGGAAGGCTCCGGTGAAGAGAATGAAGGCTCCGGCGAGGCGCATGAAGGGGATGAAGACGAAAACAGTGGCGAGTAGGAGGGGTGGGATGGTGAAAGAGTTGGACTTGAATGGTGGGTGTGGAAATGGGAATGGTGAAATGGGTGATTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

306

Amino Acids

33.62

Weight (kDa)

5.46

Isoelectric Point (pI)

47.37

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 207
AccB1I GGYRCC 2 cut(s) 207, 562
AccII CGCG 5 cut(s) 117, 143, 192, 249, 359
AcoI YGGCCR 1 cut(s) 429
AcsI RAATTY 1 cut(s) 98
AcyI GRCGYC 1 cut(s) 563
AdeI CACNNNGTG 1 cut(s) 544
AfaI GTAC 6 cut(s) 27, 209, 461, 482, 596, 629
AfiI CCNNNNNNNGG 8 cut(s) 142, 186, 239, 240, 372, 377, 395, 396
AgsI TTSAA 3 cut(s) 292, 419, 877
AhdI GACNNNNNGTC 1 cut(s) 453
AjiI CACGTC 1 cut(s) 325
AluBI AGCT 2 cut(s) 43, 277
AluI AGCT 2 cut(s) 43, 277
Alw26I GTCTC 1 cut(s) 431
Ama87I CYCGRG 3 cut(s) 29, 332, 370
AoxI GGCC 6 cut(s) 250, 354, 386, 398, 429, 554
ApaI GGGCCC 1 cut(s) 390
ApeKI GCWGC 2 cut(s) 15, 18
ApoI RAATTY 1 cut(s) 98
ArsI GACNNNNNNTTYG 2 cut(s) 359, 391
Asp700I GAANNNNTTC 1 cut(s) 42
Asp718I GGTACC 1 cut(s) 207
AspLEI GCGC 6 cut(s) 192, 286, 331, 361, 565, 810
AspS9I GGNCC 6 cut(s) 261, 340, 354, 386, 387, 398
AsuC2I CCSGG 5 cut(s) 128, 241, 296, 371, 372
AsuHPI GGTGA 4 cut(s) 742, 793, 871, 914
AvaI CYCGRG 3 cut(s) 29, 332, 370
AvaII GGWCC 2 cut(s) 261, 340
AxyI CCTNAGG 1 cut(s) 509
BaeGI GKGCMC 1 cut(s) 390
BanI GGYRCC 2 cut(s) 207, 562
BanII GRGCYC 1 cut(s) 390
BarI GAAGNNNNNNTAC 2 cut(s) 578, 610
BbsI GAAGAC 1 cut(s) 830
BbvI GCAGC 2 cut(s) 27, 30
BccI CCATC 2 cut(s) 670, 850
BceAI ACGGC 4 cut(s) 143, 297, 322, 556
BcnI CCSGG 5 cut(s) 128, 241, 296, 371, 372
BcoDI GTCTC 1 cut(s) 431
BfoI RGCGCY 3 cut(s) 287, 332, 566
BisI GCNGC 9 cut(s) 16, 19, 22, 57, 73, 76, 250, 357, 557
BlsI GCNGC 9 cut(s) 17, 20, 23, 58, 74, 77, 251, 358, 558
BmcAI AGTACT 1 cut(s) 596
Bme1390I CCNGG 5 cut(s) 128, 241, 296, 371, 372
Bme18I GGWCC 2 cut(s) 261, 340
BmeRI GACNNNNNGTC 1 cut(s) 453
BmeT110I CYCGRG 3 cut(s) 29, 332, 370
BmgBI CACGTC 1 cut(s) 325
BmgT120I GGNCC 6 cut(s) 261, 340, 354, 386, 387, 398
BmiI GGNNCC 7 cut(s) 209, 245, 263, 388, 564, 777, 798
BmrFI CCNGG 5 cut(s) 128, 241, 296, 371, 372
BmrI ACTGGG 1 cut(s) 259
BmuI ACTGGG 1 cut(s) 259
BpiI GAAGAC 1 cut(s) 830
BpuMI CCSGG 5 cut(s) 128, 241, 296, 371, 372
BsaHI GRCGYC 1 cut(s) 563
BsaJI CCNNGG 3 cut(s) 247, 370, 371
BsaWI WCCGGW 1 cut(s) 778
Bsc4I CCNNNNNNNGG 8 cut(s) 142, 186, 239, 240, 372, 377, 395, 396
Bse118I RCCGGY 1 cut(s) 559
Bse1I ACTGG 2 cut(s) 209, 265
Bse21I CCTNAGG 1 cut(s) 509
BseDI CCNNGG 3 cut(s) 247, 370, 371
BseGI GGATG 2 cut(s) 825, 861
BseLI CCNNNNNNNGG 8 cut(s) 142, 186, 239, 240, 372, 377, 395, 396
BseMII CTCAG 1 cut(s) 500
BseNI ACTGG 2 cut(s) 209, 265
BseRI GAGGAG 7 cut(s) 56, 164, 512, 695, 731, 734, 762
BseSI GKGCMC 1 cut(s) 390
BseXI GCAGC 2 cut(s) 27, 30
Bsh1236I CGCG 5 cut(s) 117, 143, 192, 249, 359
Bsh1285I CGRYCG 1 cut(s) 201
BshFI GGCC 6 cut(s) 252, 356, 388, 400, 431, 556
BshNI GGYRCC 2 cut(s) 207, 562
BsiEI CGRYCG 1 cut(s) 201
BsiHKCI CYCGRG 3 cut(s) 29, 332, 370
BslI CCNNNNNNNGG 8 cut(s) 142, 186, 239, 240, 372, 377, 395, 396
BsmAI GTCTC 1 cut(s) 431
BsmBI CGTCTC 1 cut(s) 431
BsnI GGCC 6 cut(s) 252, 356, 388, 400, 431, 556
BsoBI CYCGRG 3 cut(s) 29, 332, 370
Bsp120I GGGCCC 1 cut(s) 386
Bsp1286I GDGCHC 1 cut(s) 390
Bsp1407I TGTACA 1 cut(s) 480
Bsp143I GATC 1 cut(s) 61
BspANI GGCC 6 cut(s) 252, 356, 388, 400, 431, 556
BspCNI CTCAG 1 cut(s) 501
BspFNI CGCG 5 cut(s) 117, 143, 192, 249, 359
BspLI GGNNCC 7 cut(s) 209, 245, 263, 388, 564, 777, 798
BspT107I GGYRCC 2 cut(s) 207, 562
BsrFI RCCGGY 1 cut(s) 559
BsrGI TGTACA 1 cut(s) 480
BsrI ACTGG 2 cut(s) 209, 265
BssAI RCCGGY 1 cut(s) 559
BssECI CCNNGG 3 cut(s) 247, 370, 371
BssMI GATC 1 cut(s) 61
BssNI GRCGYC 1 cut(s) 563
Bst4CI ACNGT 2 cut(s) 451, 835
Bst6I CTCTTC 6 cut(s) 113, 179, 414, 641, 662, 779
BstACI GRCGYC 1 cut(s) 563
BstAUI TGTACA 1 cut(s) 480
BstC8I GCNNGC 2 cut(s) 115, 561
BstDEI CTNAG 1 cut(s) 509
BstDSI CCRYGG 1 cut(s) 247
BstF5I GGATG 2 cut(s) 825, 861
BstFNI CGCG 5 cut(s) 117, 143, 192, 249, 359
BstH2I RGCGCY 3 cut(s) 287, 332, 566
BstHHI GCGC 6 cut(s) 192, 286, 331, 361, 565, 810
BstKTI GATC 1 cut(s) 64
BstMAI GTCTC 1 cut(s) 431
BstMBI GATC 1 cut(s) 61
BstMCI CGRYCG 1 cut(s) 201
BstMWI GCNNNNNNNGC 5 cut(s) 21, 110, 283, 356, 562
BstNSI RCATGY 1 cut(s) 475
BstSCI CCNGG 5 cut(s) 126, 239, 294, 369, 370
BstSLI GKGCMC 1 cut(s) 390
BstUI CGCG 5 cut(s) 117, 143, 192, 249, 359
BstV1I GCAGC 2 cut(s) 27, 30
BstV2I GAAGAC 1 cut(s) 830
Bsu36I CCTNAGG 1 cut(s) 509
BsuRI GGCC 6 cut(s) 252, 356, 388, 400, 431, 556
BtgI CCRYGG 1 cut(s) 247
BtrI CACGTC 1 cut(s) 325
BtsCI GGATG 2 cut(s) 825, 861
BtsIMutI CAGTG 1 cut(s) 840
Cac8I GCNNGC 2 cut(s) 115, 561
CfoI GCGC 6 cut(s) 192, 286, 331, 361, 565, 810
Cfr10I RCCGGY 1 cut(s) 559
Cfr13I GGNCC 6 cut(s) 261, 340, 354, 386, 387, 398
Cfr42I CCGCGG 1 cut(s) 250
Cfr9I CCCGGG 1 cut(s) 370
Csp6I GTAC 6 cut(s) 26, 208, 460, 481, 595, 628
CviAII CATG 2 cut(s) 472, 811
CviQI GTAC 6 cut(s) 26, 208, 460, 481, 595, 628
DdeI CTNAG 1 cut(s) 509
DinI GGCGCC 1 cut(s) 564
DpnI GATC 1 cut(s) 63
DpnII GATC 1 cut(s) 61
DraIII CACNNNGTG 1 cut(s) 544
DriI GACNNNNNGTC 1 cut(s) 453
EaeI YGGCCR 1 cut(s) 429
Eam1104I CTCTTC 6 cut(s) 113, 179, 414, 641, 662, 779
Eam1105I GACNNNNNGTC 1 cut(s) 453
EarI CTCTTC 6 cut(s) 113, 179, 414, 641, 662, 779
Eco24I GRGCYC 1 cut(s) 390
Eco47I GGWCC 2 cut(s) 261, 340
Eco81I CCTNAGG 1 cut(s) 509
Eco88I CYCGRG 3 cut(s) 29, 332, 370
EcoT38I GRGCYC 1 cut(s) 390
EgeI GGCGCC 1 cut(s) 564
EheI GGCGCC 1 cut(s) 564
Esp3I CGTCTC 1 cut(s) 431
FaeI CATG 2 cut(s) 475, 814
FaiI YATR 6 cut(s) 468, 473, 479, 609, 623, 812
FatI CATG 2 cut(s) 471, 810
FauI CCCGC 1 cut(s) 122
Fnu4HI GCNGC 9 cut(s) 16, 19, 22, 57, 73, 76, 250, 357, 557
FokI GGATG 2 cut(s) 832, 868
FriOI GRGCYC 1 cut(s) 390
Fsp4HI GCNGC 9 cut(s) 16, 19, 22, 57, 73, 76, 250, 357, 557
GlaI GCGC 6 cut(s) 191, 285, 330, 360, 564, 809
GluI GCNGC 9 cut(s) 16, 19, 22, 57, 73, 76, 250, 357, 557
HaeII RGCGCY 3 cut(s) 287, 332, 566
HaeIII GGCC 6 cut(s) 252, 356, 388, 400, 431, 556
HhaI GCGC 6 cut(s) 192, 286, 331, 361, 565, 810
Hin1I GRCGYC 1 cut(s) 563
Hin1II CATG 2 cut(s) 475, 814
Hin6I GCGC 6 cut(s) 190, 284, 329, 359, 563, 808
HinP1I GCGC 6 cut(s) 190, 284, 329, 359, 563, 808
HindIII AAGCTT 1 cut(s) 41
HinfI GANTC 4 cut(s) 32, 51, 150, 236
HphI GGTGA 4 cut(s) 742, 793, 871, 914
Hpy188I TCNGA 9 cut(s) 12, 37, 50, 261, 322, 367, 380, 519, 709
Hpy188III TCNNGA 2 cut(s) 271, 445
Hpy99I CGWCG 4 cut(s) 143, 164, 170, 326
HpyAV CCTTC 7 cut(s) 481, 523, 580, 761, 766, 787, 808
HpyCH4III ACNGT 2 cut(s) 451, 835
HpyCH4IV ACGT 3 cut(s) 168, 324, 440
HpyCH4V TGCA 1 cut(s) 220
HpyF10VI GCNNNNNNNGC 5 cut(s) 21, 110, 283, 356, 562
HpyF3I CTNAG 1 cut(s) 509
HpySE526I ACGT 3 cut(s) 168, 324, 440
Hsp92I GRCGYC 1 cut(s) 563
Hsp92II CATG 2 cut(s) 475, 814
HspAI GCGC 6 cut(s) 190, 284, 329, 359, 563, 808
KasI GGCGCC 1 cut(s) 562
KpnI GGTACC 1 cut(s) 211
KroI GCCGGC 1 cut(s) 559
KroNI GCCGGC 1 cut(s) 561
KspI CCGCGG 1 cut(s) 250
Kzo9I GATC 1 cut(s) 61
LmnI GCTCC 3 cut(s) 589, 781, 802
Lsp1109I GCAGC 2 cut(s) 27, 30
MaeII ACGT 3 cut(s) 168, 324, 440
MalI GATC 1 cut(s) 63
MboI GATC 1 cut(s) 61
MboII GAAGA 8 cut(s) 100, 196, 431, 658, 679, 745, 796, 835
MhlI GDGCHC 1 cut(s) 390
MluCI AATT 1 cut(s) 98
Mly113I GGCGCC 1 cut(s) 563
MlyI GAGTC 2 cut(s) 41, 230
MmeI TCCRAC 3 cut(s) 345, 390, 849
MreI CGCCGGCG 1 cut(s) 559
MroNI GCCGGC 1 cut(s) 559
MroXI GAANNNNTTC 1 cut(s) 42
MslI CAYNNNNRTG 1 cut(s) 476
MspA1I CMGCKG 2 cut(s) 21, 249
MspR9I CCNGG 5 cut(s) 128, 241, 296, 371, 372
MvnI CGCG 5 cut(s) 117, 143, 192, 249, 359
MwoI GCNNNNNNNGC 5 cut(s) 21, 110, 283, 356, 562
NaeI GCCGGC 1 cut(s) 561
NarI GGCGCC 1 cut(s) 563
NciI CCSGG 5 cut(s) 128, 241, 296, 371, 372
NdeII GATC 1 cut(s) 61
NgoMIV GCCGGC 1 cut(s) 559
NlaIII CATG 2 cut(s) 475, 814
NlaIV GGNNCC 7 cut(s) 209, 245, 263, 388, 564, 777, 798
NmeAIII GCCGAG 1 cut(s) 205
NspI RCATGY 1 cut(s) 475
PcsI WCGNNNNNNNCGW 2 cut(s) 162, 455
PdiI GCCGGC 1 cut(s) 561
PdmI GAANNNNTTC 1 cut(s) 42
PfeI GAWTC 2 cut(s) 51, 150
PfoI TCCNGGA 1 cut(s) 239
PkrI GCNGC 9 cut(s) 17, 20, 23, 58, 74, 77, 251, 358, 558
PleI GAGTC 2 cut(s) 40, 230
PluTI GGCGCC 1 cut(s) 566
PpsI GAGTC 2 cut(s) 40, 230
PspN4I GGNNCC 7 cut(s) 209, 245, 263, 388, 564, 777, 798
PspOMI GGGCCC 1 cut(s) 386
PspPI GGNCC 6 cut(s) 261, 340, 354, 386, 387, 398
RsaI GTAC 6 cut(s) 27, 209, 461, 482, 596, 629
RsaNI GTAC 6 cut(s) 26, 208, 460, 481, 595, 628
RseI CAYNNNNRTG 1 cut(s) 476
SacII CCGCGG 1 cut(s) 250
SatI GCNGC 9 cut(s) 16, 19, 22, 57, 73, 76, 250, 357, 557
Sau3AI GATC 1 cut(s) 61
Sau96I GGNCC 6 cut(s) 261, 340, 354, 386, 387, 398
ScaI AGTACT 1 cut(s) 596
SchI GAGTC 2 cut(s) 41, 230
ScrFI CCNGG 5 cut(s) 128, 241, 296, 371, 372
SduI GDGCHC 1 cut(s) 390
SetI ASST 8 cut(s) 45, 171, 279, 327, 443, 492, 515, 726
SfoI GGCGCC 1 cut(s) 564
Sfr303I CCGCGG 1 cut(s) 250
SgrAI CRCCGGYG 1 cut(s) 559
SgrBI CCGCGG 1 cut(s) 250
SinI GGWCC 2 cut(s) 261, 340
SmaI CCCGGG 1 cut(s) 372
SmiMI CAYNNNNRTG 1 cut(s) 476
Sse9I AATT 1 cut(s) 98
SspDI GGCGCC 1 cut(s) 562
StyD4I CCNGG 5 cut(s) 126, 239, 294, 369, 370
TaaI ACNGT 2 cut(s) 451, 835
TaiI ACGT 3 cut(s) 171, 327, 443
TaqI TCGA 2 cut(s) 270, 761
TaqII GACCGA 1 cut(s) 215
TasI AATT 1 cut(s) 98
TatI WGTACW 3 cut(s) 480, 594, 627
TauI GCSGC 7 cut(s) 24, 59, 75, 78, 252, 359, 559
TfiI GAWTC 2 cut(s) 51, 150
TscAI CASTG 1 cut(s) 840
TseI GCWGC 2 cut(s) 15, 18
TspDTI ATGAA 3 cut(s) 806, 827, 836
TspGWI ACGGA 1 cut(s) 447
TspMI CCCGGG 1 cut(s) 370
TspRI CASTG 1 cut(s) 840
VpaK11BI GGWCC 2 cut(s) 261, 340
XapI RAATTY 1 cut(s) 98
XceI RCATGY 1 cut(s) 475
XmaI CCCGGG 1 cut(s) 370
XmnI GAANNNNTTC 1 cut(s) 42
ZrmI AGTACT 1 cut(s) 596
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.