Rh2BG288900

B3 DNA binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0000593
Physical Location & Seq
Reverse (-)
32048014 .. 32051573
3560 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG288900.1

Sequence Viewer

Length: 864 bp
ATGAGAATCCCACAGGCTTTTCGTAAGCATTTGGCTGCAAGTGTACTTCAACAATCCCATCTTCGGACCCCTGCTGGAACTTGGCTTGTTGATGTGAAAAGCGATAACGGCAGGTTCTTTTTACAAAAGGGTTGGAAGCAATTTGTGCATGATAATGGTTTAAAGCTTGGAGAGTTTCTAATTTTCCGATATGCTGGAAATTCAAAATTCTATGTTGACATATATGGAAGACATGGTTGCAAAAAGGAGTTTGTAATGCCGGCTAGGAGAAGTGAAAAACCATTTAAAAAGAGGCACGGTAATGAAAGTCATAGAACAAACGCTGTTATGGGTTCCAGCATACATCCAAAGAAGAAGATGGATTATCAGAAGTTAAAAGGTTCAGCTGGTGAAAATAAAACTAGCCAAGAAACTATGTCCGTCAAATCTGAACCAATTGATTTTGAATTGGAAACATCTATGGATATTGACGAGTCAATTAAAAATATGAATTCTAGAGAGACTTTGTCTTTCCAGCCTCAAGTGGCAAATAGTGCTCTAGAAGCAGCCAACAGATACTCATCGAACCATCCTTGCTTCAGAACTCTTTTGACCGAAACTTATGTGACCAAAGGAATTCTGCATATACCATGTACCTTTGTGAAAGATTATATTAAGATAAAGAAAAGGACAGCTGAGCTTCAAGTTTCAAACAGGTTGTGGTCTGTGAAGTGGATTAATGACTCTCGCTCAAGTAGATTCTCTCAAGGTTGGAATGAGTTTGTAAGGCAGAATGATCTGAAAGTGGGAGATGTTTGTGTCTTTGAGCTGATTAAGCAGGCGAATGATGTTGTGATGATGAAGGTTTCTTTTTTCAGATGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

287

Amino Acids

33.19

Weight (kDa)

9.75

Isoelectric Point (pI)

40.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 1 - 76 7.3e-13 B3 DNA binding domain
B3 PF02362 193 - 276 1.7e-15 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 102
AcsI RAATTY 4 cut(s) 199, 206, 490, 615
AcuI CTGAAG 1 cut(s) 562
AfaI GTAC 2 cut(s) 45, 634
AfiI CCNNNNNNNGG 1 cut(s) 63
AgsI TTSAA 5 cut(s) 50, 204, 446, 683, 690
AluBI AGCT 5 cut(s) 166, 386, 674, 679, 808
AluI AGCT 5 cut(s) 166, 386, 674, 679, 808
Alw21I GWGCWC 1 cut(s) 538
Alw26I GTCTC 1 cut(s) 494
ApeKI GCWGC 2 cut(s) 35, 545
ApoI RAATTY 4 cut(s) 199, 206, 490, 615
AseI ATTAAT 1 cut(s) 717
AspS9I GGNCC 1 cut(s) 66
AsuHPI GGTGA 1 cut(s) 401
AvaII GGWCC 1 cut(s) 66
BbsI GAAGAC 1 cut(s) 235
Bbv12I GWGCWC 1 cut(s) 538
BbvI GCAGC 2 cut(s) 22, 557
BccI CCATC 3 cut(s) 66, 352, 576
BceAI ACGGC 1 cut(s) 124
BcoDI GTCTC 1 cut(s) 494
BfaI CTAG 4 cut(s) 264, 402, 495, 539
BfuAI ACCTGC 1 cut(s) 102
BisI GCNGC 2 cut(s) 36, 546
BlpI GCTNAGC 1 cut(s) 675
BlsI GCNGC 2 cut(s) 37, 547
Bme18I GGWCC 1 cut(s) 66
BmgT120I GGNCC 1 cut(s) 66
BmiI GGNNCC 2 cut(s) 68, 334
BpiI GAAGAC 1 cut(s) 235
Bpu1102I GCTNAGC 1 cut(s) 675
BpuEI CTTGAG 3 cut(s) 504, 715, 729
BsaBI GATNNNNATC 1 cut(s) 559
Bsc4I CCNNNNNNNGG 1 cut(s) 63
Bse118I RCCGGY 1 cut(s) 259
Bse8I GATNNNNATC 1 cut(s) 559
BseGI GGATG 2 cut(s) 343, 568
BseJI GATNNNNATC 1 cut(s) 559
BseLI CCNNNNNNNGG 1 cut(s) 63
BseMII CTCAG 1 cut(s) 666
BseXI GCAGC 2 cut(s) 22, 557
BsiHKAI GWGCWC 1 cut(s) 538
BsiSI CCGG 1 cut(s) 260
BslI CCNNNNNNNGG 1 cut(s) 63
BsmAI GTCTC 1 cut(s) 494
Bsp1286I GDGCHC 1 cut(s) 538
Bsp143I GATC 1 cut(s) 775
Bsp1720I GCTNAGC 1 cut(s) 675
BspCNI CTCAG 1 cut(s) 667
BspLI GGNNCC 2 cut(s) 68, 334
BspMI ACCTGC 1 cut(s) 102
BsrFI RCCGGY 1 cut(s) 259
BssAI RCCGGY 1 cut(s) 259
BssMI GATC 1 cut(s) 775
Bst4CI ACNGT 1 cut(s) 299
BstAPI GCANNNNNTGC 2 cut(s) 145, 533
BstC8I GCNNGC 2 cut(s) 261, 819
BstDEI CTNAG 1 cut(s) 675
BstF5I GGATG 2 cut(s) 343, 568
BstKTI GATC 1 cut(s) 778
BstMAI GTCTC 1 cut(s) 494
BstMBI GATC 1 cut(s) 775
BstMWI GCNNNNNNNGC 5 cut(s) 108, 145, 533, 542, 814
BstV1I GCAGC 2 cut(s) 22, 557
BstV2I GAAGAC 1 cut(s) 235
BtsCI GGATG 2 cut(s) 343, 568
BveI ACCTGC 1 cut(s) 102
Cac8I GCNNGC 2 cut(s) 261, 819
Cfr10I RCCGGY 1 cut(s) 259
Cfr13I GGNCC 1 cut(s) 66
Csp6I GTAC 2 cut(s) 44, 633
CviAII CATG 3 cut(s) 149, 233, 630
CviQI GTAC 2 cut(s) 44, 633
DdeI CTNAG 1 cut(s) 675
DpnI GATC 1 cut(s) 777
DpnII GATC 1 cut(s) 775
DraI TTTAAA 2 cut(s) 162, 286
Eco47I GGWCC 1 cut(s) 66
Eco57I CTGAAG 1 cut(s) 562
EcoRI GAATTC 2 cut(s) 490, 615
FaeI CATG 3 cut(s) 152, 236, 633
FatI CATG 3 cut(s) 148, 232, 629
Fnu4HI GCNGC 2 cut(s) 36, 546
FokI GGATG 2 cut(s) 330, 555
Fsp4HI GCNGC 2 cut(s) 36, 546
FspBI CTAG 4 cut(s) 264, 402, 495, 539
GluI GCNGC 2 cut(s) 36, 546
HapII CCGG 1 cut(s) 260
Hin1II CATG 3 cut(s) 152, 236, 633
HincII GTYRAC 1 cut(s) 217
HindII GTYRAC 1 cut(s) 217
HindIII AAGCTT 1 cut(s) 164
HinfI GANTC 4 cut(s) 6, 473, 722, 738
HpaII CCGG 1 cut(s) 260
HphI GGTGA 1 cut(s) 401
Hpy166II GTNNAC 2 cut(s) 44, 217
Hpy188I TCNGA 7 cut(s) 66, 188, 369, 430, 581, 780, 857
Hpy188III TCNNGA 2 cut(s) 495, 539
Hpy8I GTNNAC 2 cut(s) 44, 217
HpyAV CCTTC 1 cut(s) 835
HpyCH4III ACNGT 1 cut(s) 299
HpyCH4V TGCA 4 cut(s) 38, 148, 240, 622
HpyF10VI GCNNNNNNNGC 5 cut(s) 108, 145, 533, 542, 814
HpyF3I CTNAG 1 cut(s) 675
Hsp92II CATG 3 cut(s) 152, 236, 633
KroI GCCGGC 1 cut(s) 259
KroNI GCCGGC 1 cut(s) 261
Kzo9I GATC 1 cut(s) 775
Lsp1109I GCAGC 2 cut(s) 22, 557
MaeI CTAG 4 cut(s) 264, 402, 495, 539
MaeIII GTNAC 1 cut(s) 604
MalI GATC 1 cut(s) 777
MboI GATC 1 cut(s) 775
MboII GAAGA 4 cut(s) 53, 240, 364, 367
MfeI CAATTG 1 cut(s) 435
MhlI GDGCHC 1 cut(s) 538
MluCI AATT 9 cut(s) 140, 180, 199, 206, 435, 446, 477, 490, 615
MlyI GAGTC 2 cut(s) 482, 716
MmeI TCCRAC 2 cut(s) 113, 731
MnlI CCTC 2 cut(s) 285, 528
MroNI GCCGGC 1 cut(s) 259
MseI TTAA 7 cut(s) 161, 285, 374, 480, 654, 717, 813
MslI CAYNNNNRTG 2 cut(s) 153, 300
MspA1I CMGCKG 2 cut(s) 386, 674
MspI CCGG 1 cut(s) 260
MunI CAATTG 1 cut(s) 435
MwoI GCNNNNNNNGC 5 cut(s) 108, 145, 533, 542, 814
NaeI GCCGGC 1 cut(s) 261
NdeII GATC 1 cut(s) 775
NgoMIV GCCGGC 1 cut(s) 259
NlaIII CATG 3 cut(s) 152, 236, 633
NlaIV GGNNCC 2 cut(s) 68, 334
NmuCI GTSAC 1 cut(s) 604
PdiI GCCGGC 1 cut(s) 261
PfeI GAWTC 2 cut(s) 6, 738
PflFI GACNNNGTC 1 cut(s) 505
PkrI GCNGC 2 cut(s) 37, 547
PleI GAGTC 2 cut(s) 481, 716
PpsI GAGTC 2 cut(s) 481, 716
PshBI ATTAAT 1 cut(s) 717
PspN4I GGNNCC 2 cut(s) 68, 334
PspPI GGNCC 1 cut(s) 66
PsyI GACNNNGTC 1 cut(s) 505
PvuII CAGCTG 2 cut(s) 386, 674
RsaI GTAC 2 cut(s) 45, 634
RsaNI GTAC 2 cut(s) 44, 633
RseI CAYNNNNRTG 2 cut(s) 153, 300
SaqAI TTAA 7 cut(s) 161, 285, 374, 480, 654, 717, 813
SatI GCNGC 2 cut(s) 36, 546
Sau3AI GATC 1 cut(s) 775
Sau96I GGNCC 1 cut(s) 66
SchI GAGTC 2 cut(s) 482, 716
SduI GDGCHC 1 cut(s) 538
SinI GGWCC 1 cut(s) 66
SmiMI CAYNNNNRTG 2 cut(s) 153, 300
SmlI CTYRAG 3 cut(s) 519, 730, 744
SmoI CTYRAG 3 cut(s) 519, 730, 744
Sse9I AATT 9 cut(s) 140, 180, 199, 206, 435, 446, 477, 490, 615
SspMI CTAG 4 cut(s) 264, 402, 495, 539
TaaI ACNGT 1 cut(s) 299
TaqI TCGA 1 cut(s) 563
TaqII GACCGA 1 cut(s) 608
TasI AATT 9 cut(s) 140, 180, 199, 206, 435, 446, 477, 490, 615
TatI WGTACW 1 cut(s) 43
TfiI GAWTC 2 cut(s) 6, 738
Tru1I TTAA 7 cut(s) 161, 285, 374, 480, 654, 717, 813
Tru9I TTAA 7 cut(s) 161, 285, 374, 480, 654, 717, 813
TseFI GTSAC 1 cut(s) 604
TseI GCWGC 2 cut(s) 35, 545
Tsp45I GTSAC 1 cut(s) 604
TspDTI ATGAA 3 cut(s) 318, 503, 854
TspGWI ACGGA 1 cut(s) 409
Tth111I GACNNNGTC 1 cut(s) 505
VpaK11BI GGWCC 1 cut(s) 66
VspI ATTAAT 1 cut(s) 717
XapI RAATTY 4 cut(s) 199, 206, 490, 615
XbaI TCTAGA 2 cut(s) 494, 538
XspI CTAG 4 cut(s) 264, 402, 495, 539
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.