RLG00000013545

B3 DNA binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0000593
Physical Location & Seq
Forward (+)
32929584 .. 32932263
2680 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000013545

Sequence Viewer

Length: 1044 bp
ATGGCAAGAACAACAAGACCAGTAAGAAGAAGAAAACCCTCCTTCTTCAAGGTTCTTATAGGTGACTTCTCTAACCACCTGCAAATCCCGCCGGCTTTTTACAAGCACTTTGATGGGAAGCGACCTCAGCAGACCCACCTTCGGACCCTGGCTAAAACTTGGCTTGTTAATGTGGAAATGATTGATGGCAAGTTCTTCTTCCGAAAGGGTTGGAAGAAATTTGTGCATGGCAATGGTCTGAAGCTTGGTGAATTTCTTGTTTTTCGATATTTTGGAAAATCGAAATTCTTTGTTGACATGTATGGAAGAAATACCTGCAAAAAGGATTTTGTAAAGGCCGTTGAGAAAAGTGAGAAAGAGGGACATGGTAATGAAGATAACCAGGGCTTAAAAGGTGTAGCTGAAGGAACTAGGACTGGTCAAGCCACGAACAGTATTCACATCAAAGATGAAGTAATTGATACTGAATCGGAAAGATCAAGGCAGTCAAGTTCTAGTACTAACATTGGTGGTAAAGATGTAGCACTTCAGAAAGCCAATGCTTTCAAATCTGCAGACCCTTCTTTCGTGGTTCCAATGCATATCTCGTATGTCCACGCAGGTTATATGAATGTGCCATCTGCTTTTGCCAAGGGATATCTTACTAAGCGACCTAGTGAAGTCACCCTCCAGGTTAATGGGGAAACTTGGTCTGTCAATTTACAATATGAAAGAAACAGGAAAAGACTCACCACAGGTTGGGCAGATTTTGTGCGAGCCAATAAGTTGGAAGTTGGTAACGCGTGTGTTTTTGTGTTGAGTAACGACGACAAGTCCGGCGATGCCGGAGGATACCAGACCGTAGCAGGCGATTATCGACATCGGTTCGGCCTCATCGACGACGCCAAGTCGTCGTTGCTGCTCCGGGAGAGGTCAGTTCAGCCGACTTGCTACTTCGTCGAGGAGGTCACTGAGGAGGTCATCACGGGCTTCGATGAGACCGATCTCCACTGCTCGACCCGAGGACCGGATCTGGCAAGGACCTTAGCCCCTTCGCCGGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

348

Amino Acids

39.14

Weight (kDa)

9.37

Isoelectric Point (pI)

42.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 15 - 101 1.9e-13 B3 DNA binding domain
B3 PF02362 193 - 271 1.2e-14 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 87
Acc36I ACCTGC 3 cut(s) 87, 323, 590
AccB7I CCANNNNNTGG 1 cut(s) 738
AccII CGCG 1 cut(s) 782
AciI CCGC 1 cut(s) 89
AclWI GGATC 1 cut(s) 1017
AcsI RAATTY 3 cut(s) 218, 251, 284
AcuI CTGAAG 3 cut(s) 260, 423, 512
AcyI GRCGYC 1 cut(s) 882
AfaI GTAC 1 cut(s) 499
AfiI CCNNNNNNNGG 4 cut(s) 141, 738, 1006, 1036
AflIII ACRYGT 2 cut(s) 297, 780
AgsI TTSAA 2 cut(s) 49, 547
AhdI GACNNNNNGTC 2 cut(s) 811, 886
AjnI CCWGG 3 cut(s) 147, 381, 669
AluBI AGCT 2 cut(s) 244, 401
AluI AGCT 2 cut(s) 244, 401
Alw26I GTCTC 1 cut(s) 971
AlwI GGATC 1 cut(s) 1017
Ama87I CYCGRG 1 cut(s) 999
AoxI GGCC 2 cut(s) 336, 868
ApeKI GCWGC 1 cut(s) 898
ApoI RAATTY 3 cut(s) 218, 251, 284
ArsI GACNNNNNNTTYG 2 cut(s) 548, 580
AspS9I GGNCC 3 cut(s) 144, 1004, 1020
AsuC2I CCSGG 1 cut(s) 905
AsuHPI GGTGA 4 cut(s) 74, 260, 655, 721
AvaI CYCGRG 1 cut(s) 999
AvaII GGWCC 3 cut(s) 144, 1004, 1020
BbvCI CCTCAGC 1 cut(s) 126
BbvI GCAGC 1 cut(s) 885
BccI CCATC 3 cut(s) 107, 179, 625
BceAI ACGGC 1 cut(s) 323
BciT130I CCWGG 3 cut(s) 149, 383, 671
BciVI GTATCC 1 cut(s) 824
BcnI CCSGG 1 cut(s) 905
BcoDI GTCTC 1 cut(s) 971
BfaI CTAG 3 cut(s) 411, 495, 654
BfmI CTRYAG 1 cut(s) 552
BfuAI ACCTGC 3 cut(s) 87, 323, 590
BfuI GTATCC 1 cut(s) 824
BisI GCNGC 1 cut(s) 899
BlsI GCNGC 1 cut(s) 900
BmcAI AGTACT 1 cut(s) 499
Bme1390I CCNGG 4 cut(s) 149, 383, 671, 905
Bme18I GGWCC 3 cut(s) 144, 1004, 1020
BmeRI GACNNNNNGTC 2 cut(s) 811, 886
BmeT110I CYCGRG 1 cut(s) 999
BmgT120I GGNCC 3 cut(s) 144, 1004, 1020
BmiI GGNNCC 2 cut(s) 146, 573
BmrFI CCNGG 4 cut(s) 149, 383, 671, 905
BmsI GCATC 1 cut(s) 811
BpmI CTGGAG 1 cut(s) 653
Bpu10I CCTNAGC 2 cut(s) 126, 1024
BpuMI CCSGG 1 cut(s) 905
BsaHI GRCGYC 1 cut(s) 882
BsaI GGTCTC 1 cut(s) 971
BsaJI CCNNGG 4 cut(s) 147, 382, 630, 1000
BsaWI WCCGGW 1 cut(s) 1006
Bsc4I CCNNNNNNNGG 4 cut(s) 141, 738, 1006, 1036
Bse118I RCCGGY 1 cut(s) 91
Bse1I ACTGG 2 cut(s) 20, 421
Bse3DI GCAATG 1 cut(s) 238
BseBI CCWGG 3 cut(s) 149, 383, 671
BseDI CCNNGG 4 cut(s) 147, 382, 630, 1000
BseLI CCNNNNNNNGG 4 cut(s) 141, 738, 1006, 1036
BseMI GCAATG 1 cut(s) 238
BseMII CTCAG 2 cut(s) 140, 942
BseNI ACTGG 2 cut(s) 20, 421
BseRI GAGGAG 2 cut(s) 956, 968
BseXI GCAGC 1 cut(s) 885
Bsh1236I CGCG 1 cut(s) 782
BshFI GGCC 2 cut(s) 338, 870
BsiHKCI CYCGRG 1 cut(s) 999
BsiSI CCGG 6 cut(s) 92, 816, 825, 904, 1007, 1037
BslFI GGGAC 1 cut(s) 375
BslI CCNNNNNNNGG 4 cut(s) 141, 738, 1006, 1036
BsmAI GTCTC 1 cut(s) 971
BsmFI GGGAC 1 cut(s) 375
BsnI GGCC 2 cut(s) 338, 870
Bso31I GGTCTC 1 cut(s) 971
BsoBI CYCGRG 1 cut(s) 999
Bsp143I GATC 3 cut(s) 476, 982, 1009
BspACI CCGC 1 cut(s) 89
BspANI GGCC 2 cut(s) 338, 870
BspCNI CTCAG 2 cut(s) 139, 943
BspFNI CGCG 1 cut(s) 782
BspLI GGNNCC 2 cut(s) 146, 573
BspMAI CTGCAG 1 cut(s) 556
BspMI ACCTGC 3 cut(s) 87, 323, 590
BspPI GGATC 1 cut(s) 1017
BspTNI GGTCTC 1 cut(s) 971
BsrDI GCAATG 1 cut(s) 238
BsrFI RCCGGY 1 cut(s) 91
BsrI ACTGG 2 cut(s) 20, 421
BssAI RCCGGY 1 cut(s) 91
BssECI CCNNGG 4 cut(s) 147, 382, 630, 1000
BssMI GATC 3 cut(s) 476, 982, 1009
BssNI GRCGYC 1 cut(s) 882
BssT1I CCWWGG 1 cut(s) 630
Bst2UI CCWGG 3 cut(s) 149, 383, 671
Bst4CI ACNGT 2 cut(s) 434, 841
BstACI GRCGYC 1 cut(s) 882
BstC8I GCNNGC 3 cut(s) 93, 756, 847
BstDEI CTNAG 4 cut(s) 126, 645, 951, 1024
BstFNI CGCG 1 cut(s) 782
BstKTI GATC 3 cut(s) 479, 985, 1012
BstMAI GTCTC 1 cut(s) 971
BstMBI GATC 3 cut(s) 476, 982, 1009
BstMWI GCNNNNNNNGC 2 cut(s) 88, 127
BstNI CCWGG 3 cut(s) 149, 383, 671
BstNSI RCATGY 1 cut(s) 301
BstSCI CCNGG 4 cut(s) 147, 381, 669, 903
BstSFI CTRYAG 1 cut(s) 552
BstUI CGCG 1 cut(s) 782
BstV1I GCAGC 1 cut(s) 885
BstX2I RGATCY 1 cut(s) 1009
BstXI CCANNNNNNTGG 2 cut(s) 677, 766
BstYI RGATCY 1 cut(s) 1009
BsuI GTATCC 1 cut(s) 824
BsuRI GGCC 2 cut(s) 338, 870
BtgZI GCGATG 1 cut(s) 834
BtsI GCAGTG 1 cut(s) 988
BtsIMutI CAGTG 2 cut(s) 948, 988
BveI ACCTGC 3 cut(s) 87, 323, 590
Cac8I GCNNGC 3 cut(s) 93, 756, 847
Cfr10I RCCGGY 1 cut(s) 91
Cfr13I GGNCC 3 cut(s) 144, 1004, 1020
CseI GACGC 1 cut(s) 890
Csp6I GTAC 1 cut(s) 498
CviAII CATG 3 cut(s) 227, 298, 365
CviQI GTAC 1 cut(s) 498
DdeI CTNAG 4 cut(s) 126, 645, 951, 1024
DpnI GATC 3 cut(s) 478, 984, 1011
DpnII GATC 3 cut(s) 476, 982, 1009
DriI GACNNNNNGTC 2 cut(s) 811, 886
Eam1105I GACNNNNNGTC 2 cut(s) 811, 886
Eco130I CCWWGG 1 cut(s) 630
Eco31I GGTCTC 1 cut(s) 971
Eco32I GATATC 1 cut(s) 638
Eco47I GGWCC 3 cut(s) 144, 1004, 1020
Eco57I CTGAAG 3 cut(s) 260, 423, 512
Eco88I CYCGRG 1 cut(s) 999
EcoO109I RGGNCCY 1 cut(s) 1020
EcoRII CCWGG 3 cut(s) 147, 381, 669
EcoRV GATATC 1 cut(s) 638
EcoT14I CCWWGG 1 cut(s) 630
EcoT22I ATGCAT 1 cut(s) 582
ErhI CCWWGG 1 cut(s) 630
FaeI CATG 3 cut(s) 230, 301, 368
FalI AAGNNNNNCTT 2 cut(s) 182, 214
FaqI GGGAC 1 cut(s) 375
FatI CATG 3 cut(s) 226, 297, 364
FauI CCCGC 1 cut(s) 96
Fnu4HI GCNGC 1 cut(s) 899
Fsp4HI GCNGC 1 cut(s) 899
FspBI CTAG 3 cut(s) 411, 495, 654
GluI GCNGC 1 cut(s) 899
GsuI CTGGAG 1 cut(s) 653
HaeIII GGCC 2 cut(s) 338, 870
HapII CCGG 6 cut(s) 92, 816, 825, 904, 1007, 1037
HgaI GACGC 1 cut(s) 890
Hin1I GRCGYC 1 cut(s) 882
Hin1II CATG 3 cut(s) 230, 301, 368
HincII GTYRAC 1 cut(s) 295
HindII GTYRAC 1 cut(s) 295
HindIII AAGCTT 1 cut(s) 242
HinfI GANTC 2 cut(s) 467, 726
HpaII CCGG 6 cut(s) 92, 816, 825, 904, 1007, 1037
HphI GGTGA 4 cut(s) 74, 260, 655, 721
Hpy166II GTNNAC 2 cut(s) 295, 595
Hpy188I TCNGA 5 cut(s) 144, 203, 240, 472, 531
Hpy8I GTNNAC 2 cut(s) 295, 595
Hpy99I CGWCG 5 cut(s) 809, 881, 884, 895, 941
HpyAV CCTTC 5 cut(s) 52, 149, 398, 570, 1041
HpyCH4III ACNGT 2 cut(s) 434, 841
HpyCH4V TGCA 5 cut(s) 82, 226, 318, 554, 580
HpyF10VI GCNNNNNNNGC 2 cut(s) 88, 127
HpyF3I CTNAG 4 cut(s) 126, 645, 951, 1024
Hsp92I GRCGYC 1 cut(s) 882
Hsp92II CATG 3 cut(s) 230, 301, 368
KroI GCCGGC 1 cut(s) 91
KroNI GCCGGC 1 cut(s) 93
Kzo9I GATC 3 cut(s) 476, 982, 1009
LmnI GCTCC 1 cut(s) 906
Lsp1109I GCAGC 1 cut(s) 885
LweI GCATC 1 cut(s) 811
MaeI CTAG 3 cut(s) 411, 495, 654
MaeIII GTNAC 5 cut(s) 62, 661, 776, 800, 946
MalI GATC 3 cut(s) 478, 984, 1011
MboI GATC 3 cut(s) 476, 982, 1009
MboII GAAGA 8 cut(s) 37, 39, 42, 187, 190, 226, 318, 386
MflI RGATCY 1 cut(s) 1009
MluCI AATT 5 cut(s) 218, 251, 284, 456, 697
MluI ACGCGT 1 cut(s) 780
MlyI GAGTC 1 cut(s) 720
MmeI TCCRAC 2 cut(s) 191, 747
Mph1103I ATGCAT 1 cut(s) 582
MroNI GCCGGC 1 cut(s) 91
MseI TTAA 3 cut(s) 168, 389, 675
MslI CAYNNNNRTG 3 cut(s) 111, 231, 369
MspI CCGG 6 cut(s) 92, 816, 825, 904, 1007, 1037
MspR9I CCNGG 4 cut(s) 149, 383, 671, 905
MvaI CCWGG 3 cut(s) 149, 383, 671
MvnI CGCG 1 cut(s) 782
MwoI GCNNNNNNNGC 2 cut(s) 88, 127
NaeI GCCGGC 1 cut(s) 93
NciI CCSGG 1 cut(s) 905
NdeII GATC 3 cut(s) 476, 982, 1009
NgoMIV GCCGGC 1 cut(s) 91
NlaIII CATG 3 cut(s) 230, 301, 368
NlaIV GGNNCC 2 cut(s) 146, 573
NmuCI GTSAC 3 cut(s) 62, 661, 946
NsiI ATGCAT 1 cut(s) 582
NspI RCATGY 1 cut(s) 301
PaqCI CACCTGC 1 cut(s) 87
PciI ACATGT 1 cut(s) 297
PcsI WCGNNNNNNNCGW 2 cut(s) 873, 978
PdiI GCCGGC 1 cut(s) 93
PfeI GAWTC 1 cut(s) 467
PflMI CCANNNNNTGG 1 cut(s) 738
PfoI TCCNGGA 1 cut(s) 903
PkrI GCNGC 1 cut(s) 900
PleI GAGTC 1 cut(s) 720
PpsI GAGTC 1 cut(s) 720
PpuMI RGGWCCY 1 cut(s) 1020
PscI ACATGT 1 cut(s) 297
Psp5II RGGWCCY 1 cut(s) 1020
Psp6I CCWGG 3 cut(s) 147, 381, 669
PspGI CCWGG 3 cut(s) 147, 381, 669
PspN4I GGNNCC 2 cut(s) 146, 573
PspPI GGNCC 3 cut(s) 144, 1004, 1020
PspPPI RGGWCCY 1 cut(s) 1020
PstI CTGCAG 1 cut(s) 556
PsuI RGATCY 1 cut(s) 1009
RsaI GTAC 1 cut(s) 499
RsaNI GTAC 1 cut(s) 498
RseI CAYNNNNRTG 3 cut(s) 111, 231, 369
SaqAI TTAA 3 cut(s) 168, 389, 675
SatI GCNGC 1 cut(s) 899
Sau3AI GATC 3 cut(s) 476, 982, 1009
Sau96I GGNCC 3 cut(s) 144, 1004, 1020
ScaI AGTACT 1 cut(s) 499
SchI GAGTC 1 cut(s) 720
ScrFI CCNGG 4 cut(s) 149, 383, 671, 905
SfaNI GCATC 1 cut(s) 811
SfcI CTRYAG 1 cut(s) 552
SinI GGWCC 3 cut(s) 144, 1004, 1020
SmiMI CAYNNNNRTG 3 cut(s) 111, 231, 369
Sse9I AATT 5 cut(s) 218, 251, 284, 456, 697
SsiI CCGC 1 cut(s) 89
SspMI CTAG 3 cut(s) 411, 495, 654
StyD4I CCNGG 4 cut(s) 147, 381, 669, 903
StyI CCWWGG 1 cut(s) 630
TaaI ACNGT 2 cut(s) 434, 841
TaqI TCGA 7 cut(s) 265, 281, 856, 876, 939, 972, 995
TaqII GACCGA 1 cut(s) 995
TasI AATT 5 cut(s) 218, 251, 284, 456, 697
TatI WGTACW 1 cut(s) 497
TfiI GAWTC 1 cut(s) 467
Tru1I TTAA 3 cut(s) 168, 389, 675
Tru9I TTAA 3 cut(s) 168, 389, 675
TscAI CASTG 2 cut(s) 955, 995
TseFI GTSAC 3 cut(s) 62, 661, 946
TseI GCWGC 1 cut(s) 898
Tsp45I GTSAC 3 cut(s) 62, 661, 946
TspDTI ATGAA 4 cut(s) 387, 465, 623, 723
TspRI CASTG 2 cut(s) 955, 995
Van91I CCANNNNNTGG 1 cut(s) 738
VpaK11BI GGWCC 3 cut(s) 144, 1004, 1020
XapI RAATTY 3 cut(s) 218, 251, 284
XceI RCATGY 1 cut(s) 301
XspI CTAG 3 cut(s) 411, 495, 654
ZrmI AGTACT 1 cut(s) 499
Zsp2I ATGCAT 1 cut(s) 582
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.