Rh1AG224800

B3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0000593
Physical Location & Seq
Forward (+)
42701580 .. 42715875
14296 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG224800.1

Sequence Viewer

Length: 1428 bp
ATGGCAAGAAGAAGGGATCCTTCTTTCTTCAAGATTCTTCTGGGGGATCACTTCTCTCACAAGTTGATAATTCCTGGAGCTTTTCTCAAACATTTTGATGGGAATGTGCCTGAACAGTTTCGTCTTCAAACACCAGCTAAAACTTGGCTTGTTGATGTGGAAAAGGTTGATCATAAGTACTATTTCCAAAAGGGTTGGAACGAATTTGTGAGTGAAAATGGCTTAAGGGTTAAGGAAATTCTAGTTTTTCATTATACTGGAAATTCAGACTTCTCTGTCGACATATATGGAAAAAATACTATCAAAAAGGTGTTTGTAAAAGAAAGTCATGCCTTCCCGCGATCAAAAGATGTTGGCATCCCCACAGATATTGACTATCCTATGGAAGAAGCTGATGAAGATGATGATCCGTCTGTTGAAATATTGGATGATGTTGCACCATACCCAACAAGTAGAGGAGAGAAATATCAGATATCAAGTCCTCTGCCTCACATTGATTATGATCTTGACATTCAAGATAACATATATGATGAGAATAAGCATGAAAGTTATAAAAATGAAGAAGATGAAAATGAGGAAGATGTTGATAATGAAGATGAGGATGAACATGAAAGTGAGGAAGAAGATGAAGATGAAAGTCTGAGTGAAGAAGAAAATGAAACTGCGGAAGATGTTGATTTTGGAGATGAGGATCAAAATGAAAGTGAAGAAGAAAATGAAAGTGAAGATGAGGATCACAATGATGATGATGATGATGATGATGGTGACGACAACGACGACTCTATTGAAATCTTGAGCGAAAAACCACCATGCTCGAAAACAAGAGGTAAATCTCCATTAGAATTGCAGCTCCGCAAGAGCAAGAGAACATGTTCTAATATTAAAGCTCAAAGCAATATTAAAGATGCAGGTGGCTCTTCCAGAACTGAAAGTTTCCGAAAACCTGAGATACCTAAGAAGATGAATCTAGCTATGGAAGTTGGAAAGCATAATGCTCTTCAGAGAGCTCTTGATTTTAAATCTAAATCTGTAAATCCTTGTCTTGTGCTTCCCATGCGTGCCTCTTATGTCCGTGGAAGTTACTTGAATTTTCCATTTGACTTTGCCAAGAGATATCTTAGGAAGCAGCCTAGTAACGTCACTTTTCTGGCCGATGGTGTATCTTGGCCTGTCAAGTTCTATTATCCCCAAAACCAAACCAGAGCAGTGGCTGGGTGGTCTACATTTGCGCGAGTCAATAAATTGAAGGAGAGTGATGTGTGTGTCTTTGTGTTGACTGACGACATCAAATTTGTATTTAGTGTCGCGATTTTCCGCATTACAGGTGCTGCAAATTGCACCTTACCATCACCAGTGAATCTACCACAGCCAGCACTCTTGTGGCTTAGGTTGGAGATGATTGGCCCAGATTTTGAGGCTTCCCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

475

Amino Acids

54.5

Weight (kDa)

4.55

Isoelectric Point (pI)

58.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 9 - 98 2.6e-14 B3 DNA binding domain
B3 PF02362 354 - 439 6.9e-11 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 552
AarI CACCTGC 1 cut(s) 899
AasI GACNNNNNNGTC 1 cut(s) 275
Acc36I ACCTGC 1 cut(s) 899
AccI GTMKAC 2 cut(s) 279, 1220
AccII CGCG 3 cut(s) 340, 1231, 1307
AciI CCGC 4 cut(s) 338, 665, 853, 1315
AclWI GGATC 6 cut(s) 11, 24, 54, 401, 699, 741
AcoI YGGCCR 1 cut(s) 1149
AcsI RAATTY 5 cut(s) 203, 237, 262, 1087, 1289
AcuI CTGAAG 1 cut(s) 983
AfaI GTAC 1 cut(s) 179
AflII CTTAAG 1 cut(s) 223
AflIII ACRYGT 1 cut(s) 869
AgsI TTSAA 7 cut(s) 31, 128, 419, 515, 788, 1087, 1246
AjnI CCWGG 1 cut(s) 73
AleI CACNNNNGTG 1 cut(s) 1378
AluBI AGCT 7 cut(s) 80, 137, 392, 850, 887, 971, 1007
AluI AGCT 7 cut(s) 80, 137, 392, 850, 887, 971, 1007
Alw21I GWGCWC 1 cut(s) 1009
AlwI GGATC 6 cut(s) 11, 24, 54, 401, 699, 741
AlwNI CAGNNNCTG 2 cut(s) 1211, 1328
AoxI GGCC 3 cut(s) 1149, 1166, 1402
ApeKI GCWGC 3 cut(s) 847, 1126, 1328
ApoI RAATTY 5 cut(s) 203, 237, 262, 1087, 1289
ArsI GACNNNNNNTTYG 2 cut(s) 1274, 1306
Asp700I GAANNNNTTC 2 cut(s) 117, 871
AspLEI GCGC 1 cut(s) 1231
AspS9I GGNCC 1 cut(s) 1403
AsuHPI GGTGA 2 cut(s) 776, 1341
BamHI GGATCC 1 cut(s) 16
BanII GRGCYC 1 cut(s) 1009
BbsI GAAGAC 1 cut(s) 116
Bbv12I GWGCWC 1 cut(s) 1009
BbvI GCAGC 3 cut(s) 859, 1138, 1315
BccI CCATC 4 cut(s) 92, 755, 1148, 1354
BciT130I CCWGG 1 cut(s) 75
BclI TGATCA 1 cut(s) 169
BfaI CTAG 3 cut(s) 242, 968, 1131
BfrI CTTAAG 1 cut(s) 223
BfuAI ACCTGC 1 cut(s) 899
BisI GCNGC 3 cut(s) 848, 1127, 1329
BlsI GCNGC 3 cut(s) 849, 1128, 1330
BmcAI AGTACT 1 cut(s) 179
Bme1390I CCNGG 1 cut(s) 75
BmgT120I GGNCC 1 cut(s) 1403
BmiI GGNNCC 1 cut(s) 18
BmrFI CCNGG 1 cut(s) 75
BmsI GCATC 2 cut(s) 366, 895
BpiI GAAGAC 1 cut(s) 116
BplI GAGNNNNNCTC 2 cut(s) 69, 101
BpmI CTGGAG 1 cut(s) 96
Bpu10I CCTNAGC 1 cut(s) 1385
BpuEI CTTGAG 1 cut(s) 814
BsaBI GATNNNNATC 4 cut(s) 405, 501, 690, 732
BsaJI CCNNGG 1 cut(s) 1072
Bse1I ACTGG 2 cut(s) 262, 1352
Bse8I GATNNNNATC 4 cut(s) 405, 501, 690, 732
BseBI CCWGG 1 cut(s) 75
BseDI CCNNGG 1 cut(s) 1072
BseGI GGATG 3 cut(s) 357, 433, 607
BseJI GATNNNNATC 4 cut(s) 405, 501, 690, 732
BseMII CTCAG 2 cut(s) 632, 936
BseNI ACTGG 2 cut(s) 262, 1352
BseRI GAGGAG 1 cut(s) 471
BseXI GCAGC 3 cut(s) 859, 1138, 1315
BseYI CCCAGC 1 cut(s) 1211
Bsh1236I CGCG 3 cut(s) 340, 1231, 1307
BshFI GGCC 3 cut(s) 1151, 1168, 1404
BsiHKAI GWGCWC 1 cut(s) 1009
BsnI GGCC 3 cut(s) 1151, 1168, 1404
Bsp1286I GDGCHC 1 cut(s) 1009
Bsp143I GATC 8 cut(s) 16, 46, 169, 341, 406, 502, 691, 733
Bsp68I TCGCGA 1 cut(s) 1307
BspACI CCGC 4 cut(s) 338, 665, 853, 1315
BspANI GGCC 3 cut(s) 1151, 1168, 1404
BspCNI CTCAG 2 cut(s) 633, 937
BspFNI CGCG 3 cut(s) 340, 1231, 1307
BspLI GGNNCC 1 cut(s) 18
BspMI ACCTGC 1 cut(s) 899
BspPI GGATC 6 cut(s) 11, 24, 54, 401, 699, 741
BspQI GCTCTTC 2 cut(s) 922, 1002
BspTI CTTAAG 1 cut(s) 223
BsrI ACTGG 2 cut(s) 262, 1352
BssECI CCNNGG 1 cut(s) 1072
BssMI GATC 8 cut(s) 16, 46, 169, 341, 406, 502, 691, 733
Bst2UI CCWGG 1 cut(s) 75
Bst4CI ACNGT 1 cut(s) 117
Bst6I CTCTTC 2 cut(s) 922, 1002
BstAFI CTTAAG 1 cut(s) 223
BstC8I GCNNGC 2 cut(s) 1059, 1371
BstDEI CTNAG 5 cut(s) 641, 945, 954, 1118, 1385
BstDSI CCRYGG 1 cut(s) 1072
BstF5I GGATG 3 cut(s) 357, 433, 607
BstFNI CGCG 3 cut(s) 340, 1231, 1307
BstHHI GCGC 1 cut(s) 1231
BstKTI GATC 8 cut(s) 19, 49, 172, 344, 409, 505, 694, 736
BstMBI GATC 8 cut(s) 16, 46, 169, 341, 406, 502, 691, 733
BstMWI GCNNNNNNNGC 1 cut(s) 1054
BstNI CCWGG 1 cut(s) 75
BstNSI RCATGY 1 cut(s) 873
BstSCI CCNGG 1 cut(s) 73
BstUI CGCG 3 cut(s) 340, 1231, 1307
BstV1I GCAGC 3 cut(s) 859, 1138, 1315
BstV2I GAAGAC 1 cut(s) 116
BstX2I RGATCY 1 cut(s) 16
BstXI CCANNNNNNTGG 1 cut(s) 1207
BstYI RGATCY 1 cut(s) 16
BsuRI GGCC 3 cut(s) 1151, 1168, 1404
BtgI CCRYGG 1 cut(s) 1072
BtsCI GGATG 3 cut(s) 357, 433, 607
BtsI GCAGTG 1 cut(s) 1212
BtsIMutI CAGTG 2 cut(s) 1212, 1359
BtuMI TCGCGA 1 cut(s) 1307
BveI ACCTGC 1 cut(s) 899
Cac8I GCNNGC 2 cut(s) 1059, 1371
CaiI CAGNNNCTG 2 cut(s) 1211, 1328
CfoI GCGC 1 cut(s) 1231
Cfr13I GGNCC 1 cut(s) 1403
Csp6I GTAC 1 cut(s) 178
CspCI CAANNNNNGTGG 2 cut(s) 352, 387
CviAII CATG 6 cut(s) 329, 542, 608, 810, 870, 1054
CviQI GTAC 1 cut(s) 178
DdeI CTNAG 5 cut(s) 641, 945, 954, 1118, 1385
DpnI GATC 8 cut(s) 18, 48, 171, 343, 408, 504, 693, 735
DpnII GATC 8 cut(s) 16, 46, 169, 341, 406, 502, 691, 733
DraI TTTAAA 1 cut(s) 1018
DrdI GACNNNNNNGTC 1 cut(s) 275
DseDI GACNNNNNNGTC 1 cut(s) 275
EaeI YGGCCR 1 cut(s) 1149
Eam1104I CTCTTC 2 cut(s) 922, 1002
EarI CTCTTC 2 cut(s) 922, 1002
Ecl136II GAGCTC 1 cut(s) 1007
Eco24I GRGCYC 1 cut(s) 1009
Eco32I GATATC 2 cut(s) 474, 1115
Eco53kI GAGCTC 1 cut(s) 1007
Eco57I CTGAAG 1 cut(s) 983
EcoICRI GAGCTC 1 cut(s) 1007
EcoRII CCWGG 1 cut(s) 73
EcoRV GATATC 2 cut(s) 474, 1115
EcoT38I GRGCYC 1 cut(s) 1009
FaeI CATG 6 cut(s) 332, 545, 611, 813, 873, 1057
FalI AAGNNNNNCTT 1 cut(s) 36
FatI CATG 6 cut(s) 328, 541, 607, 809, 869, 1053
FauI CCCGC 1 cut(s) 345
FbaI TGATCA 1 cut(s) 169
FblI GTMKAC 2 cut(s) 279, 1220
Fnu4HI GCNGC 3 cut(s) 848, 1127, 1329
FokI GGATG 3 cut(s) 344, 440, 614
FriOI GRGCYC 1 cut(s) 1009
Fsp4HI GCNGC 3 cut(s) 848, 1127, 1329
FspBI CTAG 3 cut(s) 242, 968, 1131
GlaI GCGC 1 cut(s) 1230
GluI GCNGC 3 cut(s) 848, 1127, 1329
GsaI CCCAGC 1 cut(s) 1215
GsuI CTGGAG 1 cut(s) 96
HaeIII GGCC 3 cut(s) 1151, 1168, 1404
HhaI GCGC 1 cut(s) 1231
Hin1II CATG 6 cut(s) 332, 545, 611, 813, 873, 1057
Hin6I GCGC 1 cut(s) 1229
HinP1I GCGC 1 cut(s) 1229
HincII GTYRAC 2 cut(s) 280, 1275
HindII GTYRAC 2 cut(s) 280, 1275
HinfI GANTC 5 cut(s) 34, 779, 964, 1233, 1357
HphI GGTGA 2 cut(s) 776, 1341
Hpy166II GTNNAC 3 cut(s) 280, 1221, 1275
Hpy188I TCNGA 5 cut(s) 268, 471, 642, 938, 1002
Hpy188III TCNNGA 7 cut(s) 31, 506, 515, 793, 921, 1010, 1306
Hpy8I GTNNAC 3 cut(s) 280, 1221, 1275
Hpy99I CGWCG 1 cut(s) 779
HpyAV CCTTC 4 cut(s) 6, 30, 343, 1240
HpyCH4III ACNGT 1 cut(s) 117
HpyCH4IV ACGT 1 cut(s) 1137
HpyCH4V TGCA 5 cut(s) 437, 847, 908, 1331, 1338
HpyF10VI GCNNNNNNNGC 1 cut(s) 1054
HpyF3I CTNAG 5 cut(s) 641, 945, 954, 1118, 1385
HpySE526I ACGT 1 cut(s) 1137
Hsp92II CATG 6 cut(s) 332, 545, 611, 813, 873, 1057
HspAI GCGC 1 cut(s) 1229
Ksp22I TGATCA 1 cut(s) 169
Kzo9I GATC 8 cut(s) 16, 46, 169, 341, 406, 502, 691, 733
LguI GCTCTTC 2 cut(s) 922, 1002
LmnI GCTCC 2 cut(s) 77, 855
Lsp1109I GCAGC 3 cut(s) 859, 1138, 1315
LweI GCATC 2 cut(s) 366, 895
MaeI CTAG 3 cut(s) 242, 968, 1131
MaeII ACGT 1 cut(s) 1137
MaeIII GTNAC 4 cut(s) 764, 1079, 1133, 1138
MalI GATC 8 cut(s) 18, 48, 171, 343, 408, 504, 693, 735
MboI GATC 8 cut(s) 16, 46, 169, 341, 406, 502, 691, 733
MflI RGATCY 1 cut(s) 16
MhlI GDGCHC 1 cut(s) 1009
MluCI AATT 9 cut(s) 69, 203, 237, 262, 842, 1087, 1241, 1289, 1333
MlyI GAGTC 2 cut(s) 773, 1242
MmeI TCCRAC 3 cut(s) 176, 961, 1371
MroXI GAANNNNTTC 2 cut(s) 117, 871
MseI TTAA 5 cut(s) 224, 231, 882, 900, 1017
MslI CAYNNNNRTG 4 cut(s) 96, 612, 741, 1378
MspCI CTTAAG 1 cut(s) 223
MspR9I CCNGG 1 cut(s) 75
MvaI CCWGG 1 cut(s) 75
MvnI CGCG 3 cut(s) 340, 1231, 1307
MwoI GCNNNNNNNGC 1 cut(s) 1054
NdeII GATC 8 cut(s) 16, 46, 169, 341, 406, 502, 691, 733
NlaIII CATG 6 cut(s) 332, 545, 611, 813, 873, 1057
NlaIV GGNNCC 1 cut(s) 18
NmuCI GTSAC 2 cut(s) 764, 1138
NruI TCGCGA 1 cut(s) 1307
NspI RCATGY 1 cut(s) 873
OliI CACNNNNGTG 1 cut(s) 1378
PaqCI CACCTGC 1 cut(s) 899
PciI ACATGT 1 cut(s) 869
PciSI GCTCTTC 2 cut(s) 922, 1002
PcsI WCGNNNNNNNCGW 1 cut(s) 774
PdmI GAANNNNTTC 2 cut(s) 117, 871
PfeI GAWTC 3 cut(s) 34, 964, 1357
PfoI TCCNGGA 1 cut(s) 73
PkrI GCNGC 3 cut(s) 849, 1128, 1330
PleI GAGTC 2 cut(s) 773, 1241
PpsI GAGTC 2 cut(s) 773, 1241
PscI ACATGT 1 cut(s) 869
PsiI TTATAA 1 cut(s) 552
Psp124BI GAGCTC 1 cut(s) 1009
Psp6I CCWGG 1 cut(s) 73
PspFI CCCAGC 1 cut(s) 1211
PspGI CCWGG 1 cut(s) 73
PspN4I GGNNCC 1 cut(s) 18
PspPI GGNCC 1 cut(s) 1403
PstNI CAGNNNCTG 2 cut(s) 1211, 1328
PsuI RGATCY 1 cut(s) 16
RruI TCGCGA 1 cut(s) 1307
RsaI GTAC 1 cut(s) 179
RsaNI GTAC 1 cut(s) 178
RseI CAYNNNNRTG 4 cut(s) 96, 612, 741, 1378
SacI GAGCTC 1 cut(s) 1009
SalI GTCGAC 1 cut(s) 278
SapI GCTCTTC 2 cut(s) 922, 1002
SaqAI TTAA 5 cut(s) 224, 231, 882, 900, 1017
SatI GCNGC 3 cut(s) 848, 1127, 1329
Sau3AI GATC 8 cut(s) 16, 46, 169, 341, 406, 502, 691, 733
Sau96I GGNCC 1 cut(s) 1403
ScaI AGTACT 1 cut(s) 179
SchI GAGTC 2 cut(s) 773, 1242
ScrFI CCNGG 1 cut(s) 75
SduI GDGCHC 1 cut(s) 1009
SfaNI GCATC 2 cut(s) 366, 895
SmiMI CAYNNNNRTG 4 cut(s) 96, 612, 741, 1378
SmlI CTYRAG 2 cut(s) 223, 793
SmoI CTYRAG 2 cut(s) 223, 793
Sse9I AATT 9 cut(s) 69, 203, 237, 262, 842, 1087, 1241, 1289, 1333
SsiI CCGC 4 cut(s) 338, 665, 853, 1315
SspI AATATT 3 cut(s) 423, 880, 898
SspMI CTAG 3 cut(s) 242, 968, 1131
SstI GAGCTC 1 cut(s) 1009
StyD4I CCNGG 1 cut(s) 73
TaaI ACNGT 1 cut(s) 117
TaiI ACGT 1 cut(s) 1140
TaqI TCGA 2 cut(s) 279, 815
TasI AATT 9 cut(s) 69, 203, 237, 262, 842, 1087, 1241, 1289, 1333
TatI WGTACW 1 cut(s) 177
TfiI GAWTC 3 cut(s) 34, 964, 1357
Tru1I TTAA 5 cut(s) 224, 231, 882, 900, 1017
Tru9I TTAA 5 cut(s) 224, 231, 882, 900, 1017
TscAI CASTG 2 cut(s) 1212, 1359
TseFI GTSAC 2 cut(s) 764, 1138
TseI GCWGC 3 cut(s) 847, 1126, 1328
Tsp45I GTSAC 2 cut(s) 764, 1138
TspGWI ACGGA 2 cut(s) 399, 1061
TspRI CASTG 2 cut(s) 1212, 1359
Vha464I CTTAAG 1 cut(s) 223
XapI RAATTY 5 cut(s) 203, 237, 262, 1087, 1289
XceI RCATGY 1 cut(s) 873
XcmI CCANNNNNNNNNTGG 2 cut(s) 141, 1377
XmiI GTMKAC 2 cut(s) 279, 1220
XmnI GAANNNNTTC 2 cut(s) 117, 871
XspI CTAG 3 cut(s) 242, 968, 1131
ZrmI AGTACT 1 cut(s) 179
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.