Rh1DG220900

B3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0000593
Physical Location & Seq
Forward (+)
43015584 .. 43019764
4181 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG220900.1

Sequence Viewer

Length: 507 bp
ATGGCAAGAAGAAGGGATCCTTCTTTCTTCAAGATTCTTCTAGGGGATCACTTCTCTCACAAGTTGATAATTCCTGGAGCTTTTCTCAAACATTTTGATGGGAATGTGCCTGAACAGTTTCGTCTTCAAACCCCAGCTAAAACTTGGCTTGTTGATGTGGAAAAGGTTGATTATAAGTACTATTTCCAAAAGGGTTGGAACGAATTTGTGCGTGAAAATGGCTTACAGGTTAAGGAAATTCTAGTTTTTCATTATACTGGAAATTCAGACTTCTCTGTCGACATATATGGAAAAAATACTATCAAAAAGGTGTTTGTAAAAGAAAGTCATGCCTTCCCGGGATCAAAAGATGTTGGCATCCCCACAGATATTGACTATCCTATGGAAGAAGCTGATGAAGATGATGATCCGTCTGTTGAAATATTGGATGATGTTGCACCATACCCAACAAGTAGAGGAGAGAAATATCAGATATCAAGTCCTCTGCCTCACATTGATTATGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

168

Amino Acids

19.45

Weight (kDa)

5.1

Isoelectric Point (pI)

40.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 9 - 98 3.8e-15 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 174
AasI GACNNNNNNGTC 1 cut(s) 275
AccI GTMKAC 1 cut(s) 279
AclWI GGATC 5 cut(s) 11, 24, 54, 349, 401
AcsI RAATTY 3 cut(s) 203, 237, 262
AfaI GTAC 1 cut(s) 179
AgsI TTSAA 3 cut(s) 31, 128, 419
AjnI CCWGG 1 cut(s) 73
AluBI AGCT 3 cut(s) 80, 137, 392
AluI AGCT 3 cut(s) 80, 137, 392
AlwI GGATC 5 cut(s) 11, 24, 54, 349, 401
Ama87I CYCGRG 1 cut(s) 337
ApoI RAATTY 3 cut(s) 203, 237, 262
Asp700I GAANNNNTTC 1 cut(s) 117
AsuC2I CCSGG 2 cut(s) 338, 339
AvaI CYCGRG 1 cut(s) 337
BamHI GGATCC 1 cut(s) 16
BbsI GAAGAC 1 cut(s) 116
BccI CCATC 1 cut(s) 92
BciT130I CCWGG 1 cut(s) 75
BcnI CCSGG 2 cut(s) 338, 339
BfaI CTAG 2 cut(s) 41, 242
BmcAI AGTACT 1 cut(s) 179
Bme1390I CCNGG 3 cut(s) 75, 338, 339
BmeT110I CYCGRG 1 cut(s) 337
BmiI GGNNCC 1 cut(s) 18
BmrFI CCNGG 3 cut(s) 75, 338, 339
BmsI GCATC 1 cut(s) 366
BpiI GAAGAC 1 cut(s) 116
BplI GAGNNNNNCTC 2 cut(s) 69, 101
BpmI CTGGAG 1 cut(s) 96
BpuMI CCSGG 2 cut(s) 338, 339
BsaBI GATNNNNATC 1 cut(s) 405
BsaJI CCNNGG 1 cut(s) 337
Bse1I ACTGG 1 cut(s) 262
Bse8I GATNNNNATC 1 cut(s) 405
BseBI CCWGG 1 cut(s) 75
BseDI CCNNGG 1 cut(s) 337
BseGI GGATG 2 cut(s) 357, 433
BseJI GATNNNNATC 1 cut(s) 405
BseNI ACTGG 1 cut(s) 262
BseRI GAGGAG 1 cut(s) 471
BseYI CCCAGC 1 cut(s) 133
BsiHKCI CYCGRG 1 cut(s) 337
BsiSI CCGG 1 cut(s) 338
BsoBI CYCGRG 1 cut(s) 337
Bsp143I GATC 4 cut(s) 16, 46, 341, 406
BspLI GGNNCC 1 cut(s) 18
BspPI GGATC 5 cut(s) 11, 24, 54, 349, 401
BsrI ACTGG 1 cut(s) 262
BssECI CCNNGG 1 cut(s) 337
BssMI GATC 4 cut(s) 16, 46, 341, 406
Bst2UI CCWGG 1 cut(s) 75
Bst4CI ACNGT 1 cut(s) 117
BstF5I GGATG 2 cut(s) 357, 433
BstKTI GATC 4 cut(s) 19, 49, 344, 409
BstMBI GATC 4 cut(s) 16, 46, 341, 406
BstNI CCWGG 1 cut(s) 75
BstSCI CCNGG 3 cut(s) 73, 336, 337
BstV2I GAAGAC 1 cut(s) 116
BstX2I RGATCY 1 cut(s) 16
BstYI RGATCY 1 cut(s) 16
BtsCI GGATG 2 cut(s) 357, 433
Cfr9I CCCGGG 1 cut(s) 337
Csp6I GTAC 1 cut(s) 178
CspCI CAANNNNNGTGG 2 cut(s) 352, 387
CviAII CATG 1 cut(s) 329
CviJI RGCY 5 cut(s) 80, 137, 148, 222, 392
CviKI_1 RGCY 5 cut(s) 80, 137, 148, 222, 392
CviQI GTAC 1 cut(s) 178
DpnI GATC 4 cut(s) 18, 48, 343, 408
DpnII GATC 4 cut(s) 16, 46, 341, 406
DrdI GACNNNNNNGTC 1 cut(s) 275
DseDI GACNNNNNNGTC 1 cut(s) 275
Eco32I GATATC 1 cut(s) 474
Eco88I CYCGRG 1 cut(s) 337
EcoRII CCWGG 1 cut(s) 73
EcoRV GATATC 1 cut(s) 474
FaeI CATG 1 cut(s) 332
FaiI YATR 9 cut(s) 174, 255, 284, 286, 288, 330, 383, 442, 501
FalI AAGNNNNNCTT 1 cut(s) 36
FatI CATG 1 cut(s) 328
FblI GTMKAC 1 cut(s) 279
FokI GGATG 2 cut(s) 344, 440
FspBI CTAG 2 cut(s) 41, 242
GsaI CCCAGC 1 cut(s) 137
GsuI CTGGAG 1 cut(s) 96
HapII CCGG 1 cut(s) 338
Hin1II CATG 1 cut(s) 332
HincII GTYRAC 1 cut(s) 280
HindII GTYRAC 1 cut(s) 280
HinfI GANTC 1 cut(s) 34
HpaII CCGG 1 cut(s) 338
Hpy166II GTNNAC 1 cut(s) 280
Hpy188I TCNGA 2 cut(s) 268, 471
Hpy188III TCNNGA 1 cut(s) 31
Hpy8I GTNNAC 1 cut(s) 280
HpyAV CCTTC 3 cut(s) 6, 30, 343
HpyCH4III ACNGT 1 cut(s) 117
HpyCH4V TGCA 1 cut(s) 437
Hsp92II CATG 1 cut(s) 332
Kzo9I GATC 4 cut(s) 16, 46, 341, 406
LmnI GCTCC 1 cut(s) 77
LpnPI CCDG 7 cut(s) 60, 87, 123, 147, 212, 243, 351
LweI GCATC 1 cut(s) 366
MaeI CTAG 2 cut(s) 41, 242
MalI GATC 4 cut(s) 18, 48, 343, 408
MboI GATC 4 cut(s) 16, 46, 341, 406
MboII GAAGA 6 cut(s) 19, 21, 29, 116, 398, 410
MflI RGATCY 1 cut(s) 16
MluCI AATT 4 cut(s) 69, 203, 237, 262
MmeI TCCRAC 1 cut(s) 176
MnlI CCTC 3 cut(s) 449, 492, 498
MroXI GAANNNNTTC 1 cut(s) 117
MseI TTAA 2 cut(s) 231, 505
MslI CAYNNNNRTG 1 cut(s) 96
MspI CCGG 1 cut(s) 338
MspR9I CCNGG 3 cut(s) 75, 338, 339
MvaI CCWGG 1 cut(s) 75
NciI CCSGG 2 cut(s) 338, 339
NdeII GATC 4 cut(s) 16, 46, 341, 406
NlaIII CATG 1 cut(s) 332
NlaIV GGNNCC 1 cut(s) 18
PdmI GAANNNNTTC 1 cut(s) 117
PfeI GAWTC 1 cut(s) 34
PfoI TCCNGGA 1 cut(s) 73
PsiI TTATAA 1 cut(s) 174
Psp6I CCWGG 1 cut(s) 73
PspFI CCCAGC 1 cut(s) 133
PspGI CCWGG 1 cut(s) 73
PspN4I GGNNCC 1 cut(s) 18
PsuI RGATCY 1 cut(s) 16
RsaI GTAC 1 cut(s) 179
RsaNI GTAC 1 cut(s) 178
RseI CAYNNNNRTG 1 cut(s) 96
SalI GTCGAC 1 cut(s) 278
SaqAI TTAA 2 cut(s) 231, 505
Sau3AI GATC 4 cut(s) 16, 46, 341, 406
ScaI AGTACT 1 cut(s) 179
ScrFI CCNGG 3 cut(s) 75, 338, 339
SetI ASST 6 cut(s) 82, 139, 168, 231, 312, 394
SfaNI GCATC 1 cut(s) 366
SmaI CCCGGG 1 cut(s) 339
SmiMI CAYNNNNRTG 1 cut(s) 96
Sse9I AATT 4 cut(s) 69, 203, 237, 262
SspI AATATT 1 cut(s) 423
SspMI CTAG 2 cut(s) 41, 242
StyD4I CCNGG 3 cut(s) 73, 336, 337
TaaI ACNGT 1 cut(s) 117
TaqI TCGA 1 cut(s) 279
TasI AATT 4 cut(s) 69, 203, 237, 262
TatI WGTACW 1 cut(s) 177
TfiI GAWTC 1 cut(s) 34
Tru1I TTAA 2 cut(s) 231, 505
Tru9I TTAA 2 cut(s) 231, 505
TspDTI ATGAA 2 cut(s) 239, 411
TspGWI ACGGA 1 cut(s) 399
TspMI CCCGGG 1 cut(s) 337
XapI RAATTY 3 cut(s) 203, 237, 262
XcmI CCANNNNNNNNNTGG 1 cut(s) 141
XmaI CCCGGG 1 cut(s) 337
XmiI GTMKAC 1 cut(s) 279
XmnI GAANNNNTTC 1 cut(s) 117
XspI CTAG 2 cut(s) 41, 242
ZrmI AGTACT 1 cut(s) 179
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.