Rorug01G0208700

B3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0000593
Physical Location & Seq
Reverse (-)
29420720 .. 29422321
1602 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0208700.1

Sequence Viewer

Length: 561 bp
ATGACAACTCCCATATTCACCTTCACATTCCTCCCTCTCTTCCTCCTCATCTCATCAACCTTCACGCCCATAATTGCCAACAATCTCATCTCTGAAACCTGCAAGAAATGTGCTCGGAACGACCCCAACCTGAGCTACAAGTTCTGCGTGACTTCTCTCCAAGCCGCCCCAAACAGCCACCGCGCCGACCTCCGGCGACTCGGCCTCATCTCCATGAAGCTAGTCCAGCACAACGTGACCAACACAAGGCACTTCATCAAGCATCTTCTCAAGAACAAGAAGCTTGACAAGTTCGTAAGGGCGCGTTTGGATGACTGTTTGGAGCTTTACACAGACTCCATACCCACCATCAAGCAAGCCATCAAGAATTACAAAAAGAAGCACAACAAAGACGCCAATATCGAAGTGAGTTCGATTGTCGATGCCTCCACGACTTGCGAAGATGGATTCGAGGAGAGACCAGGGTTGGTTTCGCCATTAACGAAAAGGAACAAAGAGACGTTTCAGCTGTGTATTATAGCGCTTTCGATTATCAATATGGTCAGTTCGTCGTTATATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

186

Amino Acids

21.12

Weight (kDa)

9.44

Isoelectric Point (pI)

41.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 28 - 177 1.5e-28 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 107
AccII CGCG 2 cut(s) 183, 304
AciI CCGC 2 cut(s) 165, 181
AcyI GRCGYC 1 cut(s) 393
AdeI CACNNNGTG 1 cut(s) 235
AfeI AGCGCT 1 cut(s) 522
AfiI CCNNNNNNNGG 2 cut(s) 192, 246
AjnI CCWGG 1 cut(s) 460
AluBI AGCT 5 cut(s) 135, 220, 283, 325, 508
AluI AGCT 5 cut(s) 135, 220, 283, 325, 508
Alw21I GWGCWC 1 cut(s) 115
Alw26I GTCTC 2 cut(s) 451, 491
Aor51HI AGCGCT 1 cut(s) 522
AoxI GGCC 1 cut(s) 202
AspLEI GCGC 3 cut(s) 185, 304, 523
AsuHPI GGTGA 1 cut(s) 10
Bbv12I GWGCWC 1 cut(s) 115
BccI CCATC 3 cut(s) 356, 368, 437
BciT130I CCWGG 1 cut(s) 462
BcoDI GTCTC 2 cut(s) 451, 491
BfaI CTAG 1 cut(s) 221
BfoI RGCGCY 1 cut(s) 524
BfuAI ACCTGC 1 cut(s) 107
BisI GCNGC 1 cut(s) 165
BlsI GCNGC 1 cut(s) 166
Bme1390I CCNGG 1 cut(s) 462
BmrFI CCNGG 1 cut(s) 462
BmsI GCATC 2 cut(s) 271, 412
Bpu10I CCTNAGC 1 cut(s) 131
BpuEI CTTGAG 1 cut(s) 254
BsaHI GRCGYC 1 cut(s) 393
BsaI GGTCTC 1 cut(s) 451
BsaJI CCNNGG 1 cut(s) 461
BsaXI ACNNNNNCTCC 2 cut(s) 320, 350
Bsc4I CCNNNNNNNGG 2 cut(s) 192, 246
BseBI CCWGG 1 cut(s) 462
BseDI CCNNGG 1 cut(s) 461
BseGI GGATG 1 cut(s) 316
BseLI CCNNNNNNNGG 2 cut(s) 192, 246
BseMII CTCAG 1 cut(s) 122
BseRI GAGGAG 2 cut(s) 35, 467
Bsh1236I CGCG 2 cut(s) 183, 304
BshFI GGCC 1 cut(s) 204
BsiHKAI GWGCWC 1 cut(s) 115
BsiSI CCGG 1 cut(s) 193
BslI CCNNNNNNNGG 2 cut(s) 192, 246
BsmAI GTCTC 2 cut(s) 451, 491
BsmBI CGTCTC 1 cut(s) 491
BsnI GGCC 1 cut(s) 204
Bso31I GGTCTC 1 cut(s) 451
Bsp1286I GDGCHC 1 cut(s) 115
BspACI CCGC 2 cut(s) 165, 181
BspANI GGCC 1 cut(s) 204
BspCNI CTCAG 1 cut(s) 123
BspFNI CGCG 2 cut(s) 183, 304
BspMI ACCTGC 1 cut(s) 107
BspTNI GGTCTC 1 cut(s) 451
BssECI CCNNGG 1 cut(s) 461
BssNI GRCGYC 1 cut(s) 393
Bst2UI CCWGG 1 cut(s) 462
Bst4CI ACNGT 1 cut(s) 317
Bst6I CTCTTC 1 cut(s) 44
BstACI GRCGYC 1 cut(s) 393
BstC8I GCNNGC 1 cut(s) 357
BstDEI CTNAG 1 cut(s) 131
BstF5I GGATG 1 cut(s) 316
BstFNI CGCG 2 cut(s) 183, 304
BstH2I RGCGCY 1 cut(s) 524
BstHHI GCGC 3 cut(s) 185, 304, 523
BstMAI GTCTC 2 cut(s) 451, 491
BstMWI GCNNNNNNNGC 1 cut(s) 226
BstNI CCWGG 1 cut(s) 462
BstSCI CCNGG 1 cut(s) 460
BstUI CGCG 2 cut(s) 183, 304
BsuRI GGCC 1 cut(s) 204
BtsCI GGATG 1 cut(s) 316
BveI ACCTGC 1 cut(s) 107
Cac8I GCNNGC 1 cut(s) 357
CfoI GCGC 3 cut(s) 185, 304, 523
CseI GACGC 1 cut(s) 401
CviAII CATG 1 cut(s) 214
CviJI RGCY 9 cut(s) 135, 164, 177, 204, 220, 283, 325, 359, 508
CviKI_1 RGCY 9 cut(s) 135, 164, 177, 204, 220, 283, 325, 359, 508
DdeI CTNAG 1 cut(s) 131
DraIII CACNNNGTG 1 cut(s) 235
Eam1104I CTCTTC 1 cut(s) 44
EarI CTCTTC 1 cut(s) 44
Eco31I GGTCTC 1 cut(s) 451
Eco47III AGCGCT 1 cut(s) 522
EcoRII CCWGG 1 cut(s) 460
Esp3I CGTCTC 1 cut(s) 491
FaeI CATG 1 cut(s) 217
FaiI YATR 7 cut(s) 14, 71, 215, 341, 518, 539, 556
FatI CATG 1 cut(s) 213
Fnu4HI GCNGC 1 cut(s) 165
FokI GGATG 1 cut(s) 323
Fsp4HI GCNGC 1 cut(s) 165
FspBI CTAG 1 cut(s) 221
GlaI GCGC 3 cut(s) 184, 303, 522
GluI GCNGC 1 cut(s) 165
HaeII RGCGCY 1 cut(s) 524
HaeIII GGCC 1 cut(s) 204
HapII CCGG 1 cut(s) 193
HgaI GACGC 1 cut(s) 401
HhaI GCGC 3 cut(s) 185, 304, 523
Hin1I GRCGYC 1 cut(s) 393
Hin1II CATG 1 cut(s) 217
Hin6I GCGC 3 cut(s) 183, 302, 521
HinP1I GCGC 3 cut(s) 183, 302, 521
HindIII AAGCTT 1 cut(s) 281
HinfI GANTC 3 cut(s) 198, 335, 447
HpaII CCGG 1 cut(s) 193
HphI GGTGA 1 cut(s) 10
Hpy188I TCNGA 2 cut(s) 94, 117
Hpy188III TCNNGA 2 cut(s) 271, 364
Hpy99I CGWCG 1 cut(s) 553
HpyAV CCTTC 2 cut(s) 31, 70
HpyCH4III ACNGT 1 cut(s) 317
HpyCH4IV ACGT 2 cut(s) 234, 500
HpyCH4V TGCA 1 cut(s) 102
HpyF10VI GCNNNNNNNGC 1 cut(s) 226
HpyF3I CTNAG 1 cut(s) 131
HpySE526I ACGT 2 cut(s) 234, 500
Hsp92I GRCGYC 1 cut(s) 393
Hsp92II CATG 1 cut(s) 217
HspAI GCGC 3 cut(s) 183, 302, 521
LmnI GCTCC 1 cut(s) 322
LpnPI CCDG 6 cut(s) 112, 143, 206, 239, 447, 474
LweI GCATC 2 cut(s) 271, 412
MaeI CTAG 1 cut(s) 221
MaeII ACGT 2 cut(s) 234, 500
MaeIII GTNAC 2 cut(s) 148, 235
MboII GAAGA 3 cut(s) 31, 257, 452
MhlI GDGCHC 1 cut(s) 115
MluCI AATT 2 cut(s) 72, 367
MlyI GAGTC 2 cut(s) 192, 329
MnlI CCTC 8 cut(s) 41, 45, 53, 56, 200, 215, 436, 445
MseI TTAA 2 cut(s) 479, 559
MslI CAYNNNNRTG 1 cut(s) 212
MspA1I CMGCKG 1 cut(s) 508
MspI CCGG 1 cut(s) 193
MspR9I CCNGG 1 cut(s) 462
MvaI CCWGG 1 cut(s) 462
MvnI CGCG 2 cut(s) 183, 304
MwoI GCNNNNNNNGC 1 cut(s) 226
NlaIII CATG 1 cut(s) 217
NmeAIII GCCGAG 1 cut(s) 180
NmuCI GTSAC 2 cut(s) 148, 235
PcsI WCGNNNNNNNCGW 2 cut(s) 399, 479
PfeI GAWTC 1 cut(s) 447
PkrI GCNGC 1 cut(s) 166
PleI GAGTC 2 cut(s) 192, 329
PpsI GAGTC 2 cut(s) 192, 329
Psp6I CCWGG 1 cut(s) 460
PspGI CCWGG 1 cut(s) 460
PvuII CAGCTG 1 cut(s) 508
RseI CAYNNNNRTG 1 cut(s) 212
SaqAI TTAA 2 cut(s) 479, 559
SatI GCNGC 1 cut(s) 165
SchI GAGTC 2 cut(s) 192, 329
ScrFI CCNGG 1 cut(s) 462
SduI GDGCHC 1 cut(s) 115
SfaNI GCATC 2 cut(s) 271, 412
SmiMI CAYNNNNRTG 1 cut(s) 212
SmlI CTYRAG 1 cut(s) 269
SmoI CTYRAG 1 cut(s) 269
Sse9I AATT 2 cut(s) 72, 367
SsiI CCGC 2 cut(s) 165, 181
SspMI CTAG 1 cut(s) 221
StyD4I CCNGG 1 cut(s) 460
TaaI ACNGT 1 cut(s) 317
TaiI ACGT 2 cut(s) 237, 503
TaqI TCGA 5 cut(s) 402, 413, 420, 450, 527
TasI AATT 2 cut(s) 72, 367
TauI GCSGC 1 cut(s) 167
TfiI GAWTC 1 cut(s) 447
Tru1I TTAA 2 cut(s) 479, 559
Tru9I TTAA 2 cut(s) 479, 559
TseFI GTSAC 2 cut(s) 148, 235
Tsp45I GTSAC 2 cut(s) 148, 235
TspDTI ATGAA 2 cut(s) 230, 244
XspI CTAG 1 cut(s) 221
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.