Rh2DG284100

B3 DNA binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0000593
Physical Location & Seq
Reverse (-)
32596016 .. 32603837
7822 bp
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UTR
Exon/CDS
Intron
Rh2DG284100.1

Sequence Viewer

Length: 687 bp
ATGGCAAGAAAAAGAAAGTCTTCCTTTTTCAAGGTTCTTATAGGTGACTTCTCTGACAAGCTGAGAATCCCACAGGCTTTTCGTAAGCATTTGGCTGCAAGTGTACTTCAACAATCCCATCTTCGGACCCCTGCTGGAACTTGGCTTGTTGATGTGAAAAGCGATAACGGCAGGTTCTTTTTACAAAAGGGTTGGAAGCAATTTGTGCATGATAATGGTTTAAAGCTTGGAGAGTTTCTAATTTTCCGATATGCTGGAAATTCAAAATTCTATGTTGACATATATGGAAGACATGGTTGCAAAAAGGAGTTTGTAATGCCGGCTAGGAGAAGTGAAAAACCATTTAAAAAGAGGCACGGTAATGAAAGTCATAGAACAAACGCTGTTATGGGTTCCAGCATACATCCAAAGAAGAAGATGGATTATCAGAAGTTAAAAGGTTCAGCTGGTGAAAATAAAACTAGCCAAGAAACTATGTCCGTCAAATCTGAACCAATTGATTTTGAATTGGAAACATCTATGGATATTGACGAGTCAATTAAAAATATGAATTCTAGAGAGACTTTGTCTTTCCAGCCTCAAGTGGCAAATAGTGCTCTAGAAGCAGCCAACAGATACTCATCGAACCATCCTTGCTTCAGAACTCTTTTGACCGAAACTTATGTGACCAAAGGAATTCTGGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

228

Amino Acids

26.11

Weight (kDa)

9.89

Isoelectric Point (pI)

37.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 9 - 96 2.3e-14 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 162
AcsI RAATTY 4 cut(s) 259, 266, 550, 675
AcuI CTGAAG 1 cut(s) 622
AfaI GTAC 1 cut(s) 105
AfiI CCNNNNNNNGG 1 cut(s) 123
AgsI TTSAA 4 cut(s) 31, 110, 264, 506
AluBI AGCT 3 cut(s) 61, 226, 446
AluI AGCT 3 cut(s) 61, 226, 446
Alw21I GWGCWC 1 cut(s) 598
Alw26I GTCTC 1 cut(s) 554
ApeKI GCWGC 2 cut(s) 95, 605
ApoI RAATTY 4 cut(s) 259, 266, 550, 675
Asp700I GAANNNNTTC 1 cut(s) 19
AspS9I GGNCC 1 cut(s) 126
AsuHPI GGTGA 2 cut(s) 56, 461
AvaII GGWCC 1 cut(s) 126
BbsI GAAGAC 2 cut(s) 12, 295
Bbv12I GWGCWC 1 cut(s) 598
BbvI GCAGC 2 cut(s) 82, 617
BccI CCATC 3 cut(s) 126, 412, 636
BceAI ACGGC 1 cut(s) 184
BcoDI GTCTC 1 cut(s) 554
BfaI CTAG 4 cut(s) 324, 462, 555, 599
BfuAI ACCTGC 1 cut(s) 162
BisI GCNGC 2 cut(s) 96, 606
BlsI GCNGC 2 cut(s) 97, 607
Bme18I GGWCC 1 cut(s) 126
BmgT120I GGNCC 1 cut(s) 126
BmiI GGNNCC 2 cut(s) 128, 394
BpiI GAAGAC 2 cut(s) 12, 295
BpuEI CTTGAG 1 cut(s) 564
BsaBI GATNNNNATC 1 cut(s) 619
Bsc4I CCNNNNNNNGG 1 cut(s) 123
Bse118I RCCGGY 1 cut(s) 319
Bse8I GATNNNNATC 1 cut(s) 619
BseGI GGATG 2 cut(s) 403, 628
BseJI GATNNNNATC 1 cut(s) 619
BseLI CCNNNNNNNGG 1 cut(s) 123
BseMII CTCAG 1 cut(s) 53
BseXI GCAGC 2 cut(s) 82, 617
BsiHKAI GWGCWC 1 cut(s) 598
BsiSI CCGG 1 cut(s) 320
BslI CCNNNNNNNGG 1 cut(s) 123
BsmAI GTCTC 1 cut(s) 554
Bsp1286I GDGCHC 1 cut(s) 598
BspCNI CTCAG 1 cut(s) 54
BspLI GGNNCC 2 cut(s) 128, 394
BspMI ACCTGC 1 cut(s) 162
BsrFI RCCGGY 1 cut(s) 319
BssAI RCCGGY 1 cut(s) 319
Bst4CI ACNGT 1 cut(s) 359
BstAPI GCANNNNNTGC 2 cut(s) 205, 593
BstC8I GCNNGC 1 cut(s) 321
BstDEI CTNAG 1 cut(s) 62
BstF5I GGATG 2 cut(s) 403, 628
BstMAI GTCTC 1 cut(s) 554
BstMWI GCNNNNNNNGC 4 cut(s) 168, 205, 593, 602
BstV1I GCAGC 2 cut(s) 82, 617
BstV2I GAAGAC 2 cut(s) 12, 295
BtsCI GGATG 2 cut(s) 403, 628
BveI ACCTGC 1 cut(s) 162
Cac8I GCNNGC 1 cut(s) 321
Cfr10I RCCGGY 1 cut(s) 319
Cfr13I GGNCC 1 cut(s) 126
Csp6I GTAC 1 cut(s) 104
CviAII CATG 2 cut(s) 209, 293
CviQI GTAC 1 cut(s) 104
DdeI CTNAG 1 cut(s) 62
DraI TTTAAA 2 cut(s) 222, 346
Eco47I GGWCC 1 cut(s) 126
Eco57I CTGAAG 1 cut(s) 622
EcoRI GAATTC 2 cut(s) 550, 675
FaeI CATG 2 cut(s) 212, 296
FalI AAGNNNNNCTT 3 cut(s) 36, 8, 40
FatI CATG 2 cut(s) 208, 292
Fnu4HI GCNGC 2 cut(s) 96, 606
FokI GGATG 2 cut(s) 390, 615
Fsp4HI GCNGC 2 cut(s) 96, 606
FspBI CTAG 4 cut(s) 324, 462, 555, 599
GluI GCNGC 2 cut(s) 96, 606
HapII CCGG 1 cut(s) 320
Hin1II CATG 2 cut(s) 212, 296
HincII GTYRAC 1 cut(s) 277
HindII GTYRAC 1 cut(s) 277
HindIII AAGCTT 1 cut(s) 224
HinfI GANTC 2 cut(s) 66, 533
HpaII CCGG 1 cut(s) 320
HphI GGTGA 2 cut(s) 56, 461
Hpy166II GTNNAC 2 cut(s) 104, 277
Hpy188I TCNGA 6 cut(s) 55, 126, 248, 429, 490, 641
Hpy188III TCNNGA 2 cut(s) 555, 599
Hpy8I GTNNAC 2 cut(s) 104, 277
HpyCH4III ACNGT 1 cut(s) 359
HpyCH4V TGCA 3 cut(s) 98, 208, 300
HpyF10VI GCNNNNNNNGC 4 cut(s) 168, 205, 593, 602
HpyF3I CTNAG 1 cut(s) 62
Hsp92II CATG 2 cut(s) 212, 296
KroI GCCGGC 1 cut(s) 319
KroNI GCCGGC 1 cut(s) 321
Lsp1109I GCAGC 2 cut(s) 82, 617
MaeI CTAG 4 cut(s) 324, 462, 555, 599
MaeIII GTNAC 2 cut(s) 44, 664
MboII GAAGA 5 cut(s) 12, 113, 300, 424, 427
MfeI CAATTG 1 cut(s) 495
MhlI GDGCHC 1 cut(s) 598
MluCI AATT 9 cut(s) 200, 240, 259, 266, 495, 506, 537, 550, 675
MlyI GAGTC 1 cut(s) 542
MmeI TCCRAC 1 cut(s) 173
MnlI CCTC 2 cut(s) 345, 588
MroNI GCCGGC 1 cut(s) 319
MroXI GAANNNNTTC 1 cut(s) 19
MseI TTAA 4 cut(s) 221, 345, 434, 540
MslI CAYNNNNRTG 2 cut(s) 213, 360
MspA1I CMGCKG 1 cut(s) 446
MspI CCGG 1 cut(s) 320
MunI CAATTG 1 cut(s) 495
MwoI GCNNNNNNNGC 4 cut(s) 168, 205, 593, 602
NaeI GCCGGC 1 cut(s) 321
NgoMIV GCCGGC 1 cut(s) 319
NlaIII CATG 2 cut(s) 212, 296
NlaIV GGNNCC 2 cut(s) 128, 394
NmuCI GTSAC 2 cut(s) 44, 664
PdiI GCCGGC 1 cut(s) 321
PdmI GAANNNNTTC 1 cut(s) 19
PfeI GAWTC 1 cut(s) 66
PflFI GACNNNGTC 1 cut(s) 565
PkrI GCNGC 2 cut(s) 97, 607
PleI GAGTC 1 cut(s) 541
PpsI GAGTC 1 cut(s) 541
PspN4I GGNNCC 2 cut(s) 128, 394
PspPI GGNCC 1 cut(s) 126
PsyI GACNNNGTC 1 cut(s) 565
PvuII CAGCTG 1 cut(s) 446
RsaI GTAC 1 cut(s) 105
RsaNI GTAC 1 cut(s) 104
RseI CAYNNNNRTG 2 cut(s) 213, 360
SaqAI TTAA 4 cut(s) 221, 345, 434, 540
SatI GCNGC 2 cut(s) 96, 606
Sau96I GGNCC 1 cut(s) 126
SchI GAGTC 1 cut(s) 542
SduI GDGCHC 1 cut(s) 598
SetI ASST 7 cut(s) 36, 46, 63, 176, 228, 442, 448
SinI GGWCC 1 cut(s) 126
SmiMI CAYNNNNRTG 2 cut(s) 213, 360
SmlI CTYRAG 1 cut(s) 579
SmoI CTYRAG 1 cut(s) 579
Sse9I AATT 9 cut(s) 200, 240, 259, 266, 495, 506, 537, 550, 675
SspMI CTAG 4 cut(s) 324, 462, 555, 599
TaaI ACNGT 1 cut(s) 359
TaqI TCGA 1 cut(s) 623
TaqII GACCGA 1 cut(s) 668
TasI AATT 9 cut(s) 200, 240, 259, 266, 495, 506, 537, 550, 675
TatI WGTACW 1 cut(s) 103
TfiI GAWTC 1 cut(s) 66
Tru1I TTAA 4 cut(s) 221, 345, 434, 540
Tru9I TTAA 4 cut(s) 221, 345, 434, 540
TseFI GTSAC 2 cut(s) 44, 664
TseI GCWGC 2 cut(s) 95, 605
Tsp45I GTSAC 2 cut(s) 44, 664
TspDTI ATGAA 2 cut(s) 378, 563
TspGWI ACGGA 1 cut(s) 469
Tth111I GACNNNGTC 1 cut(s) 565
VpaK11BI GGWCC 1 cut(s) 126
XapI RAATTY 4 cut(s) 259, 266, 550, 675
XbaI TCTAGA 2 cut(s) 554, 598
XcmI CCANNNNNNNNNTGG 1 cut(s) 676
XmnI GAANNNNTTC 1 cut(s) 19
XspI CTAG 4 cut(s) 324, 462, 555, 599
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.