Rroxscaffold_4G00305040

B3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000593
Physical Location & Seq
Reverse (-)
25689791 .. 25695350
5560 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00305040.1

Sequence Viewer

Length: 1197 bp
ATGACCAGATCATCGAGCTCGTCTCCAACTGGTGCTGCACGCCGTCAAGGAGACAAGGACCCGAGGACTCGAGGGCTCGAGTGCACTGTTTGTGACCGAGTCTCTCCAGACCAGACCAGGGCTAGAGAGAAGGGAAGAGGAAGAAGAAAGGGAAAGATCTTGATGGCAAGAAGAAGGGATCCTTCTTTCTTCAAGATTCTTCTGGGGGATCACTTCTCTCACAAGTTGATAATTCCTGGAGCTTTTGTCAAACATTTTGATGGGAATGTGCCTGAACAGTTTCGTCTTCAAACCCCAGCTAAAACTTGGCTTGTTGATGTGGAAAAGGTTGATCATAAGTACTATTTCCAAAAGGGTTGGAACGAATTTGTGCGTGAAAATGGCTTAAAGGTTAAGGAAATTCTAGTTTTTCATTATACTGGAAATTCAGACTTCTCTGTCGACATATATGGAAAAAATACTATCAAAAAGGTGTTTGTAAAAGAAAGTCATGCCTTCCCGGGATCAAAAGATGTTGGCTTCCCCACAGATATTGACTATCCTATGGAAGAAGCTGATGAAGATGATGATCCGTCTGTTGAAATATTGGATGATGTTGCACCATACCCAACAAGTAGAGGAGAGAAATGTCAGATATCAAGTGGCTCTTCCAGAACTGAAAGTTTCCGAAAACCTGAGATACCTAAGAAGATGAATCTAGCTATGGAAGTTGGAAAGCATAATGCTCTTCAGAGAGCTCTTGATTTTAAATCTAACTCTGTAAATCCTTGTCTTGTGCTTCCCATGCGTGCCTCTTATGCCCGTGGAAGTTACTTGAATTTTCCATTTGACTTTGCTAAGAGATATCTTAGGAAGCAGCCTAGTAACGTCACTTTTCTGGCCGATGGTGTATCTTGGCCTGTCAAGTTCCATTATCTCCAAAACCAAACCAGAGCAATGGCTGGCTGTGTCGCGATTTTCCGCATCACAGGTGCTGCAAATTGCACATTACCATCACCAGGACGAAGGCAAACTGCCACAGCTCAATTGAAATCGAAAAACAACAAGAGAAAGCCAGATAGAAGAAGGTTCAATAGGACTAATCTTGCAGCAACTCCTGTGAATCTTATTACACTACAGATAATTTATAGGGGTGTTGCTCTTGCTTCAGTGTTTCAGAGGCTTGTCTTTGCATATATAAAGAAGTTGCAGGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

398

Amino Acids

45.22

Weight (kDa)

9.92

Isoelectric Point (pI)

47.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 63 - 152 3.1e-15 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 437
AccI GTMKAC 1 cut(s) 441
AccII CGCG 1 cut(s) 953
AciI CCGC 1 cut(s) 961
AclWI GGATC 5 cut(s) 173, 186, 216, 511, 563
AcoI YGGCCR 1 cut(s) 879
AcsI RAATTY 4 cut(s) 365, 399, 424, 817
AcuI CTGAAG 2 cut(s) 713, 1131
AfaI GTAC 1 cut(s) 341
AfiI CCNNNNNNNGG 2 cut(s) 118, 998
AgsI TTSAA 6 cut(s) 193, 290, 581, 817, 1030, 1072
AjnI CCWGG 3 cut(s) 116, 235, 997
AluBI AGCT 7 cut(s) 18, 242, 299, 554, 701, 737, 1022
AluI AGCT 7 cut(s) 18, 242, 299, 554, 701, 737, 1022
Alw21I GWGCWC 3 cut(s) 20, 86, 739
Alw26I GTCTC 3 cut(s) 27, 45, 106
Alw44I GTGCAC 1 cut(s) 82
AlwI GGATC 5 cut(s) 173, 186, 216, 511, 563
AlwNI CAGNNNCTG 1 cut(s) 974
Ama87I CYCGRG 4 cut(s) 61, 69, 77, 499
AoxI GGCC 2 cut(s) 879, 896
ApaLI GTGCAC 1 cut(s) 82
ApeKI GCWGC 4 cut(s) 35, 856, 974, 1088
ApoI RAATTY 4 cut(s) 365, 399, 424, 817
Asp700I GAANNNNTTC 1 cut(s) 279
AspS9I GGNCC 1 cut(s) 58
AsuC2I CCSGG 2 cut(s) 500, 501
AsuHPI GGTGA 1 cut(s) 987
AvaI CYCGRG 4 cut(s) 61, 69, 77, 499
AvaII GGWCC 1 cut(s) 58
BaeGI GKGCMC 1 cut(s) 86
BamHI GGATCC 1 cut(s) 178
BanII GRGCYC 3 cut(s) 20, 78, 739
BbsI GAAGAC 1 cut(s) 278
Bbv12I GWGCWC 3 cut(s) 20, 86, 739
BbvI GCAGC 4 cut(s) 22, 868, 961, 1100
BccI CCATC 4 cut(s) 157, 254, 878, 1000
BceAI ACGGC 1 cut(s) 27
BciT130I CCWGG 3 cut(s) 118, 237, 999
BclI TGATCA 1 cut(s) 331
BcnI CCSGG 2 cut(s) 500, 501
BcoDI GTCTC 3 cut(s) 27, 45, 106
BfaI CTAG 4 cut(s) 123, 404, 698, 861
BfmI CTRYAG 1 cut(s) 1115
BglII AGATCT 1 cut(s) 156
BisI GCNGC 4 cut(s) 36, 857, 975, 1089
BlsI GCNGC 4 cut(s) 37, 858, 976, 1090
BmcAI AGTACT 1 cut(s) 341
Bme1390I CCNGG 5 cut(s) 118, 237, 500, 501, 999
Bme18I GGWCC 1 cut(s) 58
BmeT110I CYCGRG 4 cut(s) 61, 69, 77, 499
BmgT120I GGNCC 1 cut(s) 58
BmiI GGNNCC 2 cut(s) 60, 180
BmrFI CCNGG 5 cut(s) 118, 237, 500, 501, 999
BmsI GCATC 1 cut(s) 972
BpiI GAAGAC 1 cut(s) 278
BplI GAGNNNNNCTC 2 cut(s) 7, 39
BpmI CTGGAG 2 cut(s) 90, 258
BpuMI CCSGG 2 cut(s) 500, 501
BsaBI GATNNNNATC 1 cut(s) 567
BsaJI CCNNGG 4 cut(s) 62, 117, 499, 802
BsaXI ACNNNNNCTCC 2 cut(s) 231, 261
Bsc4I CCNNNNNNNGG 2 cut(s) 118, 998
Bse1I ACTGG 2 cut(s) 34, 424
Bse3DI GCAATG 1 cut(s) 942
Bse8I GATNNNNATC 1 cut(s) 567
BseBI CCWGG 3 cut(s) 118, 237, 999
BseDI CCNNGG 4 cut(s) 62, 117, 499, 802
BseGI GGATG 1 cut(s) 595
BseJI GATNNNNATC 1 cut(s) 567
BseLI CCNNNNNNNGG 2 cut(s) 118, 998
BseMI GCAATG 1 cut(s) 942
BseMII CTCAG 1 cut(s) 666
BseNI ACTGG 2 cut(s) 34, 424
BseRI GAGGAG 1 cut(s) 633
BseSI GKGCMC 1 cut(s) 86
BseXI GCAGC 4 cut(s) 22, 868, 961, 1100
BseYI CCCAGC 1 cut(s) 295
BsgI GTGCAG 1 cut(s) 21
Bsh1236I CGCG 1 cut(s) 953
BshFI GGCC 2 cut(s) 881, 898
BsiHKAI GWGCWC 3 cut(s) 20, 86, 739
BsiHKCI CYCGRG 4 cut(s) 61, 69, 77, 499
BsiSI CCGG 1 cut(s) 500
BslI CCNNNNNNNGG 2 cut(s) 118, 998
BsmAI GTCTC 3 cut(s) 27, 45, 106
BsmBI CGTCTC 1 cut(s) 27
BsnI GGCC 2 cut(s) 881, 898
BsoBI CYCGRG 4 cut(s) 61, 69, 77, 499
Bsp1286I GDGCHC 4 cut(s) 20, 78, 86, 739
Bsp143I GATC 7 cut(s) 8, 156, 178, 208, 331, 503, 568
Bsp68I TCGCGA 1 cut(s) 953
BspACI CCGC 1 cut(s) 961
BspANI GGCC 2 cut(s) 881, 898
BspCNI CTCAG 1 cut(s) 667
BspFNI CGCG 1 cut(s) 953
BspLI GGNNCC 2 cut(s) 60, 180
BspPI GGATC 5 cut(s) 173, 186, 216, 511, 563
BspQI GCTCTTC 2 cut(s) 652, 732
BsrDI GCAATG 1 cut(s) 942
BsrI ACTGG 2 cut(s) 34, 424
BssECI CCNNGG 4 cut(s) 62, 117, 499, 802
BssMI GATC 7 cut(s) 8, 156, 178, 208, 331, 503, 568
Bst2UI CCWGG 3 cut(s) 118, 237, 999
Bst4CI ACNGT 2 cut(s) 88, 279
Bst6I CTCTTC 3 cut(s) 130, 652, 732
BstC8I GCNNGC 3 cut(s) 40, 789, 943
BstDEI CTNAG 4 cut(s) 675, 684, 837, 848
BstDSI CCRYGG 1 cut(s) 802
BstF5I GGATG 1 cut(s) 595
BstFNI CGCG 1 cut(s) 953
BstKTI GATC 7 cut(s) 11, 159, 181, 211, 334, 506, 571
BstMAI GTCTC 3 cut(s) 27, 45, 106
BstMBI GATC 7 cut(s) 8, 156, 178, 208, 331, 503, 568
BstMWI GCNNNNNNNGC 2 cut(s) 784, 797
BstNI CCWGG 3 cut(s) 118, 237, 999
BstSCI CCNGG 5 cut(s) 116, 235, 498, 499, 997
BstSFI CTRYAG 1 cut(s) 1115
BstSLI GKGCMC 1 cut(s) 86
BstUI CGCG 1 cut(s) 953
BstV1I GCAGC 4 cut(s) 22, 868, 961, 1100
BstV2I GAAGAC 1 cut(s) 278
BstX2I RGATCY 2 cut(s) 156, 178
BstXI CCANNNNNNTGG 1 cut(s) 937
BstYI RGATCY 2 cut(s) 156, 178
BsuRI GGCC 2 cut(s) 881, 898
BtgI CCRYGG 1 cut(s) 802
BtsCI GGATG 1 cut(s) 595
BtsIMutI CAGTG 2 cut(s) 84, 1155
BtuMI TCGCGA 1 cut(s) 953
Cac8I GCNNGC 3 cut(s) 40, 789, 943
CaiI CAGNNNCTG 1 cut(s) 974
Cfr13I GGNCC 1 cut(s) 58
Cfr9I CCCGGG 1 cut(s) 499
Csp6I GTAC 1 cut(s) 340
CspCI CAANNNNNGTGG 2 cut(s) 514, 549
CviAII CATG 2 cut(s) 491, 784
CviQI GTAC 1 cut(s) 340
DdeI CTNAG 4 cut(s) 675, 684, 837, 848
DpnI GATC 7 cut(s) 10, 158, 180, 210, 333, 505, 570
DpnII GATC 7 cut(s) 8, 156, 178, 208, 331, 503, 568
DraI TTTAAA 1 cut(s) 748
DrdI GACNNNNNNGTC 1 cut(s) 437
DseDI GACNNNNNNGTC 1 cut(s) 437
EaeI YGGCCR 1 cut(s) 879
Eam1104I CTCTTC 3 cut(s) 130, 652, 732
EarI CTCTTC 3 cut(s) 130, 652, 732
Ecl136II GAGCTC 2 cut(s) 18, 737
Eco24I GRGCYC 3 cut(s) 20, 78, 739
Eco32I GATATC 2 cut(s) 636, 845
Eco47I GGWCC 1 cut(s) 58
Eco53kI GAGCTC 2 cut(s) 18, 737
Eco57I CTGAAG 2 cut(s) 713, 1131
Eco88I CYCGRG 4 cut(s) 61, 69, 77, 499
EcoICRI GAGCTC 2 cut(s) 18, 737
EcoO109I RGGNCCY 1 cut(s) 58
EcoRII CCWGG 3 cut(s) 116, 235, 997
EcoRV GATATC 2 cut(s) 636, 845
EcoT38I GRGCYC 3 cut(s) 20, 78, 739
Esp3I CGTCTC 1 cut(s) 27
FaeI CATG 2 cut(s) 494, 787
FalI AAGNNNNNCTT 4 cut(s) 166, 198, 631, 663
FatI CATG 2 cut(s) 490, 783
FbaI TGATCA 1 cut(s) 331
FblI GTMKAC 1 cut(s) 441
Fnu4HI GCNGC 4 cut(s) 36, 857, 975, 1089
FokI GGATG 1 cut(s) 602
FriOI GRGCYC 3 cut(s) 20, 78, 739
Fsp4HI GCNGC 4 cut(s) 36, 857, 975, 1089
FspBI CTAG 4 cut(s) 123, 404, 698, 861
GluI GCNGC 4 cut(s) 36, 857, 975, 1089
GsaI CCCAGC 1 cut(s) 299
GsuI CTGGAG 2 cut(s) 90, 258
HaeIII GGCC 2 cut(s) 881, 898
HapII CCGG 1 cut(s) 500
Hin1II CATG 2 cut(s) 494, 787
HincII GTYRAC 1 cut(s) 442
HindII GTYRAC 1 cut(s) 442
HinfI GANTC 5 cut(s) 67, 99, 196, 694, 1102
HpaII CCGG 1 cut(s) 500
HphI GGTGA 1 cut(s) 987
Hpy166II GTNNAC 2 cut(s) 84, 442
Hpy188I TCNGA 5 cut(s) 430, 633, 668, 732, 1158
Hpy188III TCNNGA 6 cut(s) 107, 160, 193, 651, 740, 952
Hpy8I GTNNAC 2 cut(s) 84, 442
HpyAV CCTTC 6 cut(s) 124, 168, 192, 505, 999, 1059
HpyCH4III ACNGT 2 cut(s) 88, 279
HpyCH4IV ACGT 1 cut(s) 867
HpyCH4V TGCA 8 cut(s) 38, 84, 599, 977, 984, 1088, 1172, 1189
HpyF10VI GCNNNNNNNGC 2 cut(s) 784, 797
HpyF3I CTNAG 4 cut(s) 675, 684, 837, 848
HpySE526I ACGT 1 cut(s) 867
Hsp92II CATG 2 cut(s) 494, 787
Ksp22I TGATCA 1 cut(s) 331
Kzo9I GATC 7 cut(s) 8, 156, 178, 208, 331, 503, 568
LguI GCTCTTC 2 cut(s) 652, 732
LmnI GCTCC 1 cut(s) 239
Lsp1109I GCAGC 4 cut(s) 22, 868, 961, 1100
LweI GCATC 1 cut(s) 972
MaeI CTAG 4 cut(s) 123, 404, 698, 861
MaeII ACGT 1 cut(s) 867
MaeIII GTNAC 4 cut(s) 92, 809, 863, 868
MalI GATC 7 cut(s) 10, 158, 180, 210, 333, 505, 570
MboI GATC 7 cut(s) 8, 156, 178, 208, 331, 503, 568
MfeI CAATTG 1 cut(s) 1025
MflI RGATCY 2 cut(s) 156, 178
MhlI GDGCHC 4 cut(s) 20, 78, 86, 739
MluCI AATT 8 cut(s) 231, 365, 399, 424, 817, 979, 1025, 1122
MlyI GAGTC 2 cut(s) 61, 108
MmeI TCCRAC 3 cut(s) 50, 338, 691
MnlI CCTC 6 cut(s) 57, 65, 131, 611, 802, 1152
MroXI GAANNNNTTC 1 cut(s) 279
MseI TTAA 3 cut(s) 386, 393, 747
MslI CAYNNNNRTG 1 cut(s) 258
MspI CCGG 1 cut(s) 500
MspR9I CCNGG 5 cut(s) 118, 237, 500, 501, 999
MunI CAATTG 1 cut(s) 1025
MvaI CCWGG 3 cut(s) 118, 237, 999
MvnI CGCG 1 cut(s) 953
MwoI GCNNNNNNNGC 2 cut(s) 784, 797
NciI CCSGG 2 cut(s) 500, 501
NdeII GATC 7 cut(s) 8, 156, 178, 208, 331, 503, 568
NlaIII CATG 2 cut(s) 494, 787
NlaIV GGNNCC 2 cut(s) 60, 180
NmuCI GTSAC 2 cut(s) 92, 868
NruI TCGCGA 1 cut(s) 953
PaeR7I CTCGAG 2 cut(s) 69, 77
PciSI GCTCTTC 2 cut(s) 652, 732
PdmI GAANNNNTTC 1 cut(s) 279
PfeI GAWTC 3 cut(s) 196, 694, 1102
PflFI GACNNNGTC 1 cut(s) 98
PfoI TCCNGGA 1 cut(s) 235
PkrI GCNGC 4 cut(s) 37, 858, 976, 1090
PleI GAGTC 2 cut(s) 61, 107
PpsI GAGTC 2 cut(s) 61, 107
PpuMI RGGWCCY 1 cut(s) 58
Psp124BI GAGCTC 2 cut(s) 20, 739
Psp5II RGGWCCY 1 cut(s) 58
Psp6I CCWGG 3 cut(s) 116, 235, 997
PspFI CCCAGC 1 cut(s) 295
PspGI CCWGG 3 cut(s) 116, 235, 997
PspN4I GGNNCC 2 cut(s) 60, 180
PspPI GGNCC 1 cut(s) 58
PspPPI RGGWCCY 1 cut(s) 58
PspXI VCTCGAGB 2 cut(s) 69, 77
PstNI CAGNNNCTG 1 cut(s) 974
PsuI RGATCY 2 cut(s) 156, 178
PsyI GACNNNGTC 1 cut(s) 98
RruI TCGCGA 1 cut(s) 953
RsaI GTAC 1 cut(s) 341
RsaNI GTAC 1 cut(s) 340
RseI CAYNNNNRTG 1 cut(s) 258
SacI GAGCTC 2 cut(s) 20, 739
SalI GTCGAC 1 cut(s) 440
SapI GCTCTTC 2 cut(s) 652, 732
SaqAI TTAA 3 cut(s) 386, 393, 747
SatI GCNGC 4 cut(s) 36, 857, 975, 1089
Sau3AI GATC 7 cut(s) 8, 156, 178, 208, 331, 503, 568
Sau96I GGNCC 1 cut(s) 58
ScaI AGTACT 1 cut(s) 341
SchI GAGTC 2 cut(s) 61, 108
ScrFI CCNGG 5 cut(s) 118, 237, 500, 501, 999
SduI GDGCHC 4 cut(s) 20, 78, 86, 739
SfaNI GCATC 1 cut(s) 972
SfcI CTRYAG 1 cut(s) 1115
Sfr274I CTCGAG 2 cut(s) 69, 77
SinI GGWCC 1 cut(s) 58
SlaI CTCGAG 2 cut(s) 69, 77
SmaI CCCGGG 1 cut(s) 501
SmiMI CAYNNNNRTG 1 cut(s) 258
SmlI CTYRAG 2 cut(s) 69, 77
SmoI CTYRAG 2 cut(s) 69, 77
Sse9I AATT 8 cut(s) 231, 365, 399, 424, 817, 979, 1025, 1122
SsiI CCGC 1 cut(s) 961
SspI AATATT 1 cut(s) 585
SspMI CTAG 4 cut(s) 123, 404, 698, 861
SstI GAGCTC 2 cut(s) 20, 739
StyD4I CCNGG 5 cut(s) 116, 235, 498, 499, 997
TaaI ACNGT 2 cut(s) 88, 279
TaiI ACGT 1 cut(s) 870
TaqI TCGA 5 cut(s) 14, 70, 78, 441, 1034
TaqII GACCGA 1 cut(s) 111
TasI AATT 8 cut(s) 231, 365, 399, 424, 817, 979, 1025, 1122
TatI WGTACW 1 cut(s) 339
TfiI GAWTC 3 cut(s) 196, 694, 1102
Tru1I TTAA 3 cut(s) 386, 393, 747
Tru9I TTAA 3 cut(s) 386, 393, 747
TscAI CASTG 2 cut(s) 91, 1155
TseFI GTSAC 2 cut(s) 92, 868
TseI GCWGC 4 cut(s) 35, 856, 974, 1088
Tsp45I GTSAC 2 cut(s) 92, 868
TspDTI ATGAA 3 cut(s) 401, 573, 707
TspGWI ACGGA 1 cut(s) 561
TspMI CCCGGG 1 cut(s) 499
TspRI CASTG 2 cut(s) 91, 1155
Tth111I GACNNNGTC 1 cut(s) 98
VneI GTGCAC 1 cut(s) 82
VpaK11BI GGWCC 1 cut(s) 58
XapI RAATTY 4 cut(s) 365, 399, 424, 817
XcmI CCANNNNNNNNNTGG 1 cut(s) 303
XhoI CTCGAG 2 cut(s) 69, 77
XmaI CCCGGG 1 cut(s) 499
XmiI GTMKAC 1 cut(s) 441
XmnI GAANNNNTTC 1 cut(s) 279
XspI CTAG 4 cut(s) 123, 404, 698, 861
ZrmI AGTACT 1 cut(s) 341
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.