FvH4_1g23530

B3 DNA binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0000593
Physical Location & Seq
Forward (+)
15366471 .. 15367566
1096 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g23530.t1

Sequence Viewer

Length: 723 bp
ATGACAAGAAAAAGAAAGCCCTCCTTTTTCAAGGTTCTTATAGGTGACTTCTCTGACCAGCTGAGCATCCCACAAGCTTTCCATAAGCATTTTGATGGAAGGGTACCTCAGCAGTCCCATCTTCAGACCCCGACTGGAACTTGGTTTGTTGATGTGAAAAGTGATAATGGGAGGTTGTTTTTACAAAAGGGTTGGAAGCAATTTGTGCGTGATAATGGTTTAAGGCTTGGCGAGTTTCTAGTTTTTCGATACGCTGGAAATTCAAAATTCTTTGTTGACATATATGCGAGACATGGCTGCAAAAAGGAACTTATAATGGCGGCTACGGGAAGTGAAAGACCTCTTGTAAAGAGACAGAGTGAAGAGATTCATAGAACAAATGCTAACTCGAAGAAGTATGTTGGCAGCTCCACGAGACATCCAAAGCAGAACATGGATAATGAGAACTTAGAAGGTTCCACTGGTGGATATAAAGCTGGTCAAGAAACTGTTACCGTCAAATCTGAACCTATTAGTGATAGTGAATTGAAAACATCTGTGGATCTTGATGAGTCTATTAGAAATATGAATACTAGAGAGACTTTGGCTTCACAGCCTCAAGTGGAAAATCGAACACAAGCTACTGCTCTTGAAGCAGCCAACAAATGTGCATCGAAGCATCCTTGCTTCAGAGTTATTTTGACTCAAAATTATGTGACCAAAGGAATGCTGGTAGGAACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

241

Amino Acids

27.12

Weight (kDa)

9.63

Isoelectric Point (pI)

33.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 9 - 96 7.9e-15 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 314
Acc65I GGTACC 1 cut(s) 103
AccB1I GGYRCC 1 cut(s) 103
AciI CCGC 1 cut(s) 320
AclWI GGATC 1 cut(s) 549
AcsI RAATTY 2 cut(s) 259, 266
AcuI CTGAAG 2 cut(s) 107, 652
AfaI GTAC 1 cut(s) 105
AgsI TTSAA 4 cut(s) 31, 264, 529, 632
AluBI AGCT 5 cut(s) 61, 77, 408, 476, 620
AluI AGCT 5 cut(s) 61, 77, 408, 476, 620
Alw26I GTCTC 4 cut(s) 283, 346, 409, 572
AlwI GGATC 1 cut(s) 549
ApeKI GCWGC 3 cut(s) 297, 405, 635
ApoI RAATTY 2 cut(s) 259, 266
Asp700I GAANNNNTTC 1 cut(s) 366
Asp718I GGTACC 1 cut(s) 103
AsuHPI GGTGA 1 cut(s) 56
BanI GGYRCC 1 cut(s) 103
BauI CACGAG 1 cut(s) 412
BbvCI CCTCAGC 1 cut(s) 108
BbvI GCAGC 3 cut(s) 284, 417, 647
BccI CCATC 2 cut(s) 89, 126
BcoDI GTCTC 4 cut(s) 283, 346, 409, 572
BfaI CTAG 2 cut(s) 239, 573
BisI GCNGC 4 cut(s) 298, 321, 406, 636
BlpI GCTNAGC 1 cut(s) 62
BlsI GCNGC 4 cut(s) 299, 322, 407, 637
BmiI GGNNCC 2 cut(s) 105, 457
BmsI GCATC 3 cut(s) 75, 659, 667
Bpu10I CCTNAGC 1 cut(s) 108
Bpu1102I GCTNAGC 1 cut(s) 62
BpuEI CTTGAG 1 cut(s) 582
Bse1I ACTGG 2 cut(s) 139, 466
BseGI GGATG 3 cut(s) 66, 418, 658
BseMII CTCAG 2 cut(s) 53, 122
BseNI ACTGG 2 cut(s) 139, 466
BseXI GCAGC 3 cut(s) 284, 417, 647
BshNI GGYRCC 1 cut(s) 103
BslFI GGGAC 1 cut(s) 100
BsmAI GTCTC 4 cut(s) 283, 346, 409, 572
BsmFI GGGAC 1 cut(s) 100
BsmI GAATGC 1 cut(s) 711
Bsp143I GATC 1 cut(s) 541
Bsp1720I GCTNAGC 1 cut(s) 62
BspACI CCGC 1 cut(s) 320
BspCNI CTCAG 2 cut(s) 54, 121
BspLI GGNNCC 2 cut(s) 105, 457
BspPI GGATC 1 cut(s) 549
BspT107I GGYRCC 1 cut(s) 103
BsrI ACTGG 2 cut(s) 139, 466
BssMI GATC 1 cut(s) 541
BssSI CACGAG 1 cut(s) 412
Bst2BI CACGAG 1 cut(s) 412
Bst4CI ACNGT 2 cut(s) 490, 496
Bst6I CTCTTC 1 cut(s) 357
BstAPI GCANNNNNTGC 1 cut(s) 205
BstDEI CTNAG 3 cut(s) 62, 108, 448
BstF5I GGATG 3 cut(s) 66, 418, 658
BstKTI GATC 1 cut(s) 544
BstMAI GTCTC 4 cut(s) 283, 346, 409, 572
BstMBI GATC 1 cut(s) 541
BstMWI GCNNNNNNNGC 2 cut(s) 205, 632
BstV1I GCAGC 3 cut(s) 284, 417, 647
BstX2I RGATCY 1 cut(s) 541
BstYI RGATCY 1 cut(s) 541
BtsCI GGATG 3 cut(s) 66, 418, 658
BtsIMutI CAGTG 1 cut(s) 459
Csp6I GTAC 1 cut(s) 104
CviAII CATG 3 cut(s) 293, 433, 720
CviQI GTAC 1 cut(s) 104
DdeI CTNAG 3 cut(s) 62, 108, 448
DpnI GATC 1 cut(s) 543
DpnII GATC 1 cut(s) 541
Eam1104I CTCTTC 1 cut(s) 357
EarI CTCTTC 1 cut(s) 357
Eco57I CTGAAG 2 cut(s) 107, 652
FaeI CATG 3 cut(s) 296, 436, 723
FalI AAGNNNNNCTT 2 cut(s) 8, 40
FaqI GGGAC 1 cut(s) 100
FatI CATG 3 cut(s) 292, 432, 719
Fnu4HI GCNGC 4 cut(s) 298, 321, 406, 636
FokI GGATG 3 cut(s) 53, 405, 645
Fsp4HI GCNGC 4 cut(s) 298, 321, 406, 636
FspBI CTAG 2 cut(s) 239, 573
GluI GCNGC 4 cut(s) 298, 321, 406, 636
Hin1II CATG 3 cut(s) 296, 436, 723
HincII GTYRAC 1 cut(s) 277
HindII GTYRAC 1 cut(s) 277
HindIII AAGCTT 1 cut(s) 75
HinfI GANTC 3 cut(s) 367, 551, 682
HphI GGTGA 1 cut(s) 56
Hpy166II GTNNAC 1 cut(s) 277
Hpy188I TCNGA 4 cut(s) 55, 126, 505, 671
Hpy188III TCNNGA 3 cut(s) 482, 545, 629
Hpy8I GTNNAC 1 cut(s) 277
HpyAV CCTTC 2 cut(s) 93, 446
HpyCH4III ACNGT 2 cut(s) 490, 496
HpyCH4V TGCA 2 cut(s) 300, 650
HpyF10VI GCNNNNNNNGC 2 cut(s) 205, 632
HpyF3I CTNAG 3 cut(s) 62, 108, 448
Hsp92II CATG 3 cut(s) 296, 436, 723
KpnI GGTACC 1 cut(s) 107
Kzo9I GATC 1 cut(s) 541
LmnI GCTCC 1 cut(s) 413
LpnPI CCDG 6 cut(s) 71, 120, 240, 447, 462, 695
Lsp1109I GCAGC 3 cut(s) 284, 417, 647
LweI GCATC 3 cut(s) 75, 659, 667
MaeI CTAG 2 cut(s) 239, 573
MaeIII GTNAC 3 cut(s) 44, 490, 694
MalI GATC 1 cut(s) 543
MboI GATC 1 cut(s) 541
MboII GAAGA 3 cut(s) 113, 374, 403
MflI RGATCY 1 cut(s) 541
MluCI AATT 5 cut(s) 200, 259, 266, 524, 688
MlyI GAGTC 2 cut(s) 560, 676
MmeI TCCRAC 1 cut(s) 173
MnlI CCTC 5 cut(s) 31, 117, 165, 351, 606
MroXI GAANNNNTTC 1 cut(s) 366
MseI TTAA 1 cut(s) 221
MslI CAYNNNNRTG 1 cut(s) 93
MspA1I CMGCKG 1 cut(s) 61
Mva1269I GAATGC 1 cut(s) 711
MwoI GCNNNNNNNGC 2 cut(s) 205, 632
NdeII GATC 1 cut(s) 541
NlaIII CATG 3 cut(s) 296, 436, 723
NlaIV GGNNCC 2 cut(s) 105, 457
NmuCI GTSAC 2 cut(s) 44, 694
PctI GAATGC 1 cut(s) 711
PdmI GAANNNNTTC 1 cut(s) 366
PfeI GAWTC 1 cut(s) 367
PkrI GCNGC 4 cut(s) 299, 322, 407, 637
PleI GAGTC 2 cut(s) 559, 676
PpsI GAGTC 2 cut(s) 559, 676
PsiI TTATAA 1 cut(s) 314
PspN4I GGNNCC 2 cut(s) 105, 457
PsrI GAACNNNNNNTAC 2 cut(s) 604, 636
PsuI RGATCY 1 cut(s) 541
PvuII CAGCTG 1 cut(s) 61
RsaI GTAC 1 cut(s) 105
RsaNI GTAC 1 cut(s) 104
RseI CAYNNNNRTG 1 cut(s) 93
SaqAI TTAA 1 cut(s) 221
SatI GCNGC 4 cut(s) 298, 321, 406, 636
Sau3AI GATC 1 cut(s) 541
SchI GAGTC 2 cut(s) 560, 676
SfaNI GCATC 3 cut(s) 75, 659, 667
SmiMI CAYNNNNRTG 1 cut(s) 93
SmlI CTYRAG 1 cut(s) 597
SmoI CTYRAG 1 cut(s) 597
Sse9I AATT 5 cut(s) 200, 259, 266, 524, 688
SsiI CCGC 1 cut(s) 320
SspMI CTAG 2 cut(s) 239, 573
TaaI ACNGT 2 cut(s) 490, 496
TaqI TCGA 4 cut(s) 247, 389, 610, 653
TasI AATT 5 cut(s) 200, 259, 266, 524, 688
TauI GCSGC 1 cut(s) 323
TfiI GAWTC 1 cut(s) 367
Tru1I TTAA 1 cut(s) 221
Tru9I TTAA 1 cut(s) 221
TscAI CASTG 1 cut(s) 466
TseFI GTSAC 2 cut(s) 44, 694
TseI GCWGC 3 cut(s) 297, 405, 635
Tsp45I GTSAC 2 cut(s) 44, 694
TspDTI ATGAA 2 cut(s) 359, 581
TspRI CASTG 1 cut(s) 466
XapI RAATTY 2 cut(s) 259, 266
XcmI CCANNNNNNNNNTGG 1 cut(s) 706
XmnI GAANNNNTTC 1 cut(s) 366
XspI CTAG 2 cut(s) 239, 573
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.