RchiOBHm_Chr2g0103391

B3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0000593
Physical Location & Seq
Reverse (-)
14961232 .. 14964191
2960 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ47775

Sequence Viewer

Length: 1401 bp
ATGGCAACAACAAGAAAACCCTCTTTCTTCAAGGTTCTTATAGGGGATCACTTCTGTAACAGATTGAAAATTCCCGGAGCTTTTCTTGAGCATTTTGATGGGAGAGTACCTGAACAGTGCCATCTTAGAATCGGCACTCAGACTTGGCTTGTTGATGTTGAAATGGTCCATCAGAAGTTCTATTTCCAAAATGGTTGGAAGGAATTTGTGCTTGAGAATGGTTTAAGGGGTTGCGAGTTTCTAGTGTTTTTCTATAATGGAAATTCGGAGTTCTATGTCCACATATATGGGAAGAATGCTTGCAGAAAGGAGTTTGCAGATGCCGCTGCTGGAGTAAGTGGCACACCGAAATCAATGCATGTTGGTAGCTCCAGAGGGATGGAAGAAGCTGATAGTAATGACTCATCTGTTCAGATCTTGGATGACTTTCCAACCTGCTCAAGAAGAGCAGGGGAGAAATCTTCATTACCAAGTCATCAGCAGCACAAGAAAGACAGTACAAGCTGGATTGGTAAAGAGACACATGGTCCCAAACCAGGAATTACAAGAAAAGATAATGATTTTCCAGCGGAACAATATGTGGAAGGGACTTCTAGCTTGAAACCAAAACATGAGGTTCTTGGGAGGATGCACACTGATAACGACGATGATGACAAAGAAGAGGAAGAGAGTGAGGAGGATGATGATGATTATGAGGAAGATGAAGAGATTGAGGAGGATGAAAATGAAGCTGACAAAAGCGATGGAGATGGGGGGAAAAGTGATGATTCTATTGAAATTCTGGACGAGTTTCCACGATGCTCAAAAGCAAAAACAATGAGGAAATCATCAGAGCTTCAGAAGAGAAAGATAAACAGTTCTAAAGCTAAAACAAAAAGAAGCATAACACGTGCCAGTGGCTCGTCTAGCAATCAAAAAATCACAGGATGGAAATATAAGGCTTTAAGGAAAATGCCTGGATCTAATGGAATCGGAGAACTTATAGCCCGTCACAGAGCTAATGATTTCATATCCAAATCTGACAAGCTGGATTCTTGTTTGATTGCCATGTCTCCTTCGTATATTAGAGGATCTTGTGTGCATTTTCCACGTACTTTTGCCTACAGACATCTTGGAAGGCATTGTAGTCGCATCAAACTTCGGGTTAATAAGAGGAGGTGGCGTCTTGGTATCTATCATGTCCATAAAACAGTTAGATTACAAGCTGGATGGATTGAATTTAAGCATGACAATAATCTGAAAGTGGGTGATGTGTGCGTCTTTGAGTTGACTGACAGCATCAATGTACTTGATGTTACAATTTTCCGCTCCGCAGAAGCTGCAAATTGCACCTTGCCAGGGGGAAGACAAACGACTGGGGTTCAATTCAAGTCGGAAAAAGGAAAAGAGAAAGCTGGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

466

Amino Acids

52.78

Weight (kDa)

7.27

Isoelectric Point (pI)

51.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 9 - 98 7.6e-11 B3 DNA binding domain
B3 PF02362 351 - 437 1.5e-10 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 443
AccBSI CCGCTC 1 cut(s) 1308
AciI CCGC 4 cut(s) 324, 569, 1306, 1311
AclWI GGATC 3 cut(s) 54, 967, 1078
AcsI RAATTY 5 cut(s) 69, 203, 262, 777, 1217
AcuI CTGAAG 1 cut(s) 821
AcvI CACGTG 1 cut(s) 890
AcyI GRCGYC 1 cut(s) 1162
AfaI GTAC 4 cut(s) 108, 499, 1093, 1287
AfiI CCNNNNNNNGG 2 cut(s) 536, 1338
AflIII ACRYGT 1 cut(s) 887
AgsI TTSAA 8 cut(s) 31, 67, 161, 601, 776, 1217, 1364, 1369
AhdI GACNNNNNGTC 1 cut(s) 525
AjnI CCWGG 3 cut(s) 535, 955, 1336
Alw26I GTCTC 2 cut(s) 512, 1056
AlwI GGATC 3 cut(s) 54, 967, 1078
AlwNI CAGNNNCTG 1 cut(s) 1319
ApeKI GCWGC 3 cut(s) 326, 481, 1319
ApoI RAATTY 5 cut(s) 69, 203, 262, 777, 1217
AspS9I GGNCC 2 cut(s) 166, 527
AsuC2I CCSGG 1 cut(s) 75
AsuHPI GGTGA 1 cut(s) 1259
AvaII GGWCC 2 cut(s) 166, 527
BbrPI CACGTG 1 cut(s) 890
BbsI GAAGAC 1 cut(s) 1351
BbvI GCAGC 3 cut(s) 313, 493, 1306
BccI CCATC 8 cut(s) 92, 129, 177, 373, 737, 743, 921, 1203
BcgI CGANNNNNNTGC 4 cut(s) 337, 371, 1109, 1143
BciT130I CCWGG 3 cut(s) 537, 957, 1338
BcnI CCSGG 1 cut(s) 75
BcoDI GTCTC 2 cut(s) 512, 1056
BfaI CTAG 3 cut(s) 242, 594, 906
BfmI CTRYAG 1 cut(s) 1102
BfuAI ACCTGC 1 cut(s) 443
BglII AGATCT 1 cut(s) 414
BisI GCNGC 4 cut(s) 324, 327, 482, 1320
BlsI GCNGC 4 cut(s) 325, 328, 483, 1321
Bme1390I CCNGG 4 cut(s) 75, 537, 957, 1338
Bme18I GGWCC 2 cut(s) 166, 527
BmeRI GACNNNNNGTC 1 cut(s) 525
BmgT120I GGNCC 2 cut(s) 166, 527
BmiI GGNNCC 1 cut(s) 529
BmrFI CCNGG 4 cut(s) 75, 537, 957, 1338
BmrI ACTGGG 1 cut(s) 1365
BmsI GCATC 5 cut(s) 310, 618, 788, 1140, 1287
BmuI ACTGGG 1 cut(s) 1365
BpiI GAAGAC 1 cut(s) 1351
BpmI CTGGAG 2 cut(s) 351, 355
BpuEI CTTGAG 3 cut(s) 107, 233, 424
BpuMI CCSGG 1 cut(s) 75
BsaAI YACGTR 2 cut(s) 890, 1091
BsaHI GRCGYC 1 cut(s) 1162
BsaJI CCNNGG 1 cut(s) 1337
BsaXI ACNNNNNCTCC 2 cut(s) 1147, 1177
Bsc4I CCNNNNNNNGG 2 cut(s) 536, 1338
Bse1I ACTGG 2 cut(s) 894, 1360
BseBI CCWGG 3 cut(s) 537, 957, 1338
BseDI CCNNGG 1 cut(s) 1337
BseGI GGATG 7 cut(s) 384, 427, 633, 685, 724, 932, 1214
BseLI CCNNNNNNNGG 2 cut(s) 536, 1338
BseMII CTCAG 1 cut(s) 152
BseNI ACTGG 2 cut(s) 894, 1360
BseRI GAGGAG 3 cut(s) 689, 728, 1168
BseXI GCAGC 3 cut(s) 313, 493, 1306
BsiSI CCGG 1 cut(s) 75
BslFI GGGAC 2 cut(s) 513, 601
BslI CCNNNNNNNGG 2 cut(s) 536, 1338
BsmAI GTCTC 2 cut(s) 512, 1056
BsmFI GGGAC 2 cut(s) 513, 601
BsmI GAATGC 1 cut(s) 301
Bsp143I GATC 4 cut(s) 46, 414, 959, 1070
BspACI CCGC 4 cut(s) 324, 569, 1306, 1311
BspCNI CTCAG 1 cut(s) 151
BspLI GGNNCC 1 cut(s) 529
BspMI ACCTGC 1 cut(s) 443
BspPI GGATC 3 cut(s) 54, 967, 1078
BspQI GCTCTTC 1 cut(s) 439
BsrBI CCGCTC 1 cut(s) 1308
BsrI ACTGG 2 cut(s) 894, 1360
BssECI CCNNGG 1 cut(s) 1337
BssMI GATC 4 cut(s) 46, 414, 959, 1070
BssNI GRCGYC 1 cut(s) 1162
Bst2UI CCWGG 3 cut(s) 537, 957, 1338
Bst4CI ACNGT 4 cut(s) 117, 497, 857, 1192
Bst6I CTCTTC 5 cut(s) 439, 654, 660, 699, 836
BstACI GRCGYC 1 cut(s) 1162
BstAPI GCANNNNNTGC 1 cut(s) 1319
BstBAI YACGTR 2 cut(s) 890, 1091
BstC8I GCNNGC 1 cut(s) 301
BstDEI CTNAG 2 cut(s) 125, 138
BstF5I GGATG 7 cut(s) 384, 427, 633, 685, 724, 932, 1214
BstKTI GATC 4 cut(s) 49, 417, 962, 1073
BstMAI GTCTC 2 cut(s) 512, 1056
BstMBI GATC 4 cut(s) 46, 414, 959, 1070
BstMWI GCNNNNNNNGC 3 cut(s) 323, 906, 1319
BstNI CCWGG 3 cut(s) 537, 957, 1338
BstNSI RCATGY 1 cut(s) 362
BstSCI CCNGG 4 cut(s) 73, 535, 955, 1336
BstSFI CTRYAG 1 cut(s) 1102
BstV1I GCAGC 3 cut(s) 313, 493, 1306
BstV2I GAAGAC 1 cut(s) 1351
BstX2I RGATCY 3 cut(s) 414, 959, 1070
BstXI CCANNNNNNTGG 2 cut(s) 287, 379
BstYI RGATCY 3 cut(s) 414, 959, 1070
BtgZI GCGATG 1 cut(s) 756
BtsCI GGATG 7 cut(s) 384, 427, 633, 685, 724, 932, 1214
BtsIMutI CAGTG 3 cut(s) 122, 633, 901
BveI ACCTGC 1 cut(s) 443
Cac8I GCNNGC 1 cut(s) 301
CaiI CAGNNNCTG 1 cut(s) 1319
Cfr13I GGNCC 2 cut(s) 166, 527
CseI GACGC 2 cut(s) 1151, 1246
Csp6I GTAC 4 cut(s) 107, 498, 1092, 1286
CviAII CATG 6 cut(s) 359, 524, 611, 1048, 1178, 1226
CviQI GTAC 4 cut(s) 107, 498, 1092, 1286
DdeI CTNAG 2 cut(s) 125, 138
DpnI GATC 4 cut(s) 48, 416, 961, 1072
DpnII GATC 4 cut(s) 46, 414, 959, 1070
DriI GACNNNNNGTC 1 cut(s) 525
Eam1104I CTCTTC 5 cut(s) 439, 654, 660, 699, 836
Eam1105I GACNNNNNGTC 1 cut(s) 525
EarI CTCTTC 5 cut(s) 439, 654, 660, 699, 836
Eco47I GGWCC 2 cut(s) 166, 527
Eco57I CTGAAG 1 cut(s) 821
Eco72I CACGTG 1 cut(s) 890
EcoRII CCWGG 3 cut(s) 535, 955, 1336
EcoT22I ATGCAT 1 cut(s) 360
FaeI CATG 6 cut(s) 362, 527, 614, 1051, 1181, 1229
FaqI GGGAC 2 cut(s) 513, 601
FatI CATG 6 cut(s) 358, 523, 610, 1047, 1177, 1225
Fnu4HI GCNGC 4 cut(s) 324, 327, 482, 1320
FokI GGATG 7 cut(s) 391, 434, 640, 692, 731, 939, 1221
Fsp4HI GCNGC 4 cut(s) 324, 327, 482, 1320
FspBI CTAG 3 cut(s) 242, 594, 906
GluI GCNGC 4 cut(s) 324, 327, 482, 1320
GsuI CTGGAG 2 cut(s) 351, 355
HapII CCGG 1 cut(s) 75
HgaI GACGC 2 cut(s) 1151, 1246
Hin1I GRCGYC 1 cut(s) 1162
Hin1II CATG 6 cut(s) 362, 527, 614, 1051, 1181, 1229
HincII GTYRAC 1 cut(s) 1269
HindII GTYRAC 1 cut(s) 1269
HinfI GANTC 5 cut(s) 129, 401, 767, 969, 1031
HpaII CCGG 1 cut(s) 75
HphI GGTGA 1 cut(s) 1259
Hpy166II GTNNAC 2 cut(s) 280, 1269
Hpy188III TCNNGA 4 cut(s) 86, 372, 441, 782
Hpy8I GTNNAC 2 cut(s) 280, 1269
Hpy99I CGWCG 1 cut(s) 647
HpyAV CCTTC 4 cut(s) 193, 578, 1065, 1110
HpyCH4III ACNGT 4 cut(s) 117, 497, 857, 1192
HpyCH4IV ACGT 2 cut(s) 889, 1090
HpyCH4V TGCA 7 cut(s) 303, 317, 358, 631, 1081, 1322, 1329
HpyF10VI GCNNNNNNNGC 3 cut(s) 323, 906, 1319
HpyF3I CTNAG 2 cut(s) 125, 138
HpySE526I ACGT 2 cut(s) 889, 1090
Hsp92I GRCGYC 1 cut(s) 1162
Hsp92II CATG 6 cut(s) 362, 527, 614, 1051, 1181, 1229
Kzo9I GATC 4 cut(s) 46, 414, 959, 1070
LguI GCTCTTC 1 cut(s) 439
LmnI GCTCC 3 cut(s) 77, 374, 1313
Lsp1109I GCAGC 3 cut(s) 313, 493, 1306
LweI GCATC 5 cut(s) 310, 618, 788, 1140, 1287
MaeI CTAG 3 cut(s) 242, 594, 906
MaeII ACGT 2 cut(s) 889, 1090
MaeIII GTNAC 3 cut(s) 56, 989, 1294
MalI GATC 4 cut(s) 48, 416, 961, 1072
MbiI CCGCTC 1 cut(s) 1308
MboI GATC 4 cut(s) 46, 414, 959, 1070
MflI RGATCY 3 cut(s) 414, 959, 1070
MluCI AATT 9 cut(s) 69, 203, 262, 540, 777, 1217, 1299, 1324, 1364
MlyI GAGTC 1 cut(s) 395
MmeI TCCRAC 3 cut(s) 176, 455, 1353
Mph1103I ATGCAT 1 cut(s) 360
MseI TTAA 4 cut(s) 224, 944, 1146, 1221
MslI CAYNNNNRTG 2 cut(s) 96, 285
MspA1I CMGCKG 2 cut(s) 326, 569
MspI CCGG 1 cut(s) 75
MspR9I CCNGG 4 cut(s) 75, 537, 957, 1338
Mva1269I GAATGC 1 cut(s) 301
MvaI CCWGG 3 cut(s) 537, 957, 1338
MwoI GCNNNNNNNGC 3 cut(s) 323, 906, 1319
NciI CCSGG 1 cut(s) 75
NdeII GATC 4 cut(s) 46, 414, 959, 1070
NlaIII CATG 6 cut(s) 362, 527, 614, 1051, 1181, 1229
NlaIV GGNNCC 1 cut(s) 529
NmuCI GTSAC 1 cut(s) 989
NsiI ATGCAT 1 cut(s) 360
NspI RCATGY 1 cut(s) 362
PciSI GCTCTTC 1 cut(s) 439
PctI GAATGC 1 cut(s) 301
PfeI GAWTC 4 cut(s) 129, 767, 969, 1031
PfoI TCCNGGA 1 cut(s) 73
PkrI GCNGC 4 cut(s) 325, 328, 483, 1321
PleI GAGTC 1 cut(s) 395
PmaCI CACGTG 1 cut(s) 890
PmlI CACGTG 1 cut(s) 890
PpsI GAGTC 1 cut(s) 395
Ppu21I YACGTR 2 cut(s) 890, 1091
Psp6I CCWGG 3 cut(s) 535, 955, 1336
PspCI CACGTG 1 cut(s) 890
PspGI CCWGG 3 cut(s) 535, 955, 1336
PspN4I GGNNCC 1 cut(s) 529
PspPI GGNCC 2 cut(s) 166, 527
PstNI CAGNNNCTG 1 cut(s) 1319
PsuI RGATCY 3 cut(s) 414, 959, 1070
RsaI GTAC 4 cut(s) 108, 499, 1093, 1287
RsaNI GTAC 4 cut(s) 107, 498, 1092, 1286
RseI CAYNNNNRTG 2 cut(s) 96, 285
SapI GCTCTTC 1 cut(s) 439
SaqAI TTAA 4 cut(s) 224, 944, 1146, 1221
SatI GCNGC 4 cut(s) 324, 327, 482, 1320
Sau3AI GATC 4 cut(s) 46, 414, 959, 1070
Sau96I GGNCC 2 cut(s) 166, 527
SchI GAGTC 1 cut(s) 395
ScrFI CCNGG 4 cut(s) 75, 537, 957, 1338
SfaNI GCATC 5 cut(s) 310, 618, 788, 1140, 1287
SfcI CTRYAG 1 cut(s) 1102
SinI GGWCC 2 cut(s) 166, 527
SmiMI CAYNNNNRTG 2 cut(s) 96, 285
SmlI CTYRAG 3 cut(s) 86, 212, 439
SmoI CTYRAG 3 cut(s) 86, 212, 439
Sse9I AATT 9 cut(s) 69, 203, 262, 540, 777, 1217, 1299, 1324, 1364
SsiI CCGC 4 cut(s) 324, 569, 1306, 1311
SspMI CTAG 3 cut(s) 242, 594, 906
StyD4I CCNGG 4 cut(s) 73, 535, 955, 1336
TaaI ACNGT 4 cut(s) 117, 497, 857, 1192
TaiI ACGT 2 cut(s) 892, 1093
TasI AATT 9 cut(s) 69, 203, 262, 540, 777, 1217, 1299, 1324, 1364
TatI WGTACW 2 cut(s) 497, 1285
TauI GCSGC 1 cut(s) 326
TfiI GAWTC 4 cut(s) 129, 767, 969, 1031
Tru1I TTAA 4 cut(s) 224, 944, 1146, 1221
Tru9I TTAA 4 cut(s) 224, 944, 1146, 1221
TscAI CASTG 3 cut(s) 122, 640, 901
TseFI GTSAC 1 cut(s) 989
TseI GCWGC 3 cut(s) 326, 481, 1319
Tsp45I GTSAC 1 cut(s) 989
TspDTI ATGAA 5 cut(s) 453, 717, 735, 741, 997
TspRI CASTG 3 cut(s) 122, 640, 901
VpaK11BI GGWCC 2 cut(s) 166, 527
XapI RAATTY 5 cut(s) 69, 203, 262, 777, 1217
XceI RCATGY 1 cut(s) 362
XspI CTAG 3 cut(s) 242, 594, 906
Zsp2I ATGCAT 1 cut(s) 360
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.