Prupe.7G144100_v2.0.a1

B3 DNA binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp07
OG0000593
Physical Location & Seq
Forward (+)
15802002 .. 15805466
3465 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.7G144100.1

Sequence Viewer

Length: 996 bp
ATGCATCCGTTGGCTGCAACGACTGGGAAAGCTCTTGCTCTTCGCAGAGACATTGGTTCTGAATCTGAAAACCCTTCTTTTGAGGTTGCAATGTGGTTCTCCTACATCCGATTTGCCGGCTTTAGTTTGTCAACGATCATCTCGCTGAGCAGCCTAGTTCGGGTTTCGGATGGGAGAACTTGGCCTGTCAATAAAGCTCTCCTCAGTCCAGAAGAGGAAGAAGATAGGGTTCAAACAGGAAACAGGAAACAAGAGAAAGAAAATGGTGATGGCAAGAAGAAACCCCTCCTTCTTCAGGTTCTTGTAGGTGACTCCTCTAACCAGTTGTCCCACCTTTGGACCTCAGCTGGAACTTGGGCTGTTAATGTGAAGAAGGTTAATGACAAGTTCTTTCTCCAAAAGGGTTGGAAGAAATTTGTCCATGACCATGATTTAAAGCTTTGTGAGTTTCTAGTCTTTGATTACGCTGGAAATTTGGGATTCTATGTTGACATTTATGGAAGAGATGGCTGCAAAAAAAAGTTTGTAACAGCCATTAGGGAAAAAGGCCGCAGACCTCATGAAGAGGGACATGGTAATGTAATTCACAGAAGGAGCCCAAGACATCATGGAAAACAGAATTTGCCTTCAATTGATCCCATCAAATATGAAGCGATCGATACTGAATCTGAAACATCAATGCATCCATTGGCTACAAGTACTGGAGAAACTATTCCTCTTCAGCGAAACAATAGCTTCAACTCTGAAAAACCTTTTTTCAAGATTGCAATACAGAACTCGTACATCCGTAGAGGTTATATTTATTTGCCACGTAATTTTGTCAAGACACATCTCACCGAGCAGCGAGCTACTGTTACTCTTCGGGTTTTGGATGGGAAAACTTGGCCTGTCAAGTTGGCATTTGAAGGTGAAAGAGGCAAACTCGGTGGTGGTTTCATGACATTTTGCCGAGATAATAAATTAGAAGTTGGTGACGTGTGTCTTTGTCTTGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

332

Amino Acids

37.56

Weight (kDa)

9.29

Isoelectric Point (pI)

39.63

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 550
AclWI GGATC 1 cut(s) 629
AcsI RAATTY 3 cut(s) 413, 472, 619
AcuI CTGAAG 2 cut(s) 278, 704
AfaI GTAC 2 cut(s) 700, 782
AfiI CCNNNNNNNGG 3 cut(s) 160, 295, 336
AflIII ACRYGT 1 cut(s) 975
AgsI TTSAA 5 cut(s) 233, 630, 739, 760, 905
AjiI CACGTC 1 cut(s) 976
AluBI AGCT 6 cut(s) 32, 197, 347, 439, 735, 848
AluI AGCT 6 cut(s) 32, 197, 347, 439, 735, 848
Alw26I GTCTC 1 cut(s) 42
AlwI GGATC 1 cut(s) 629
AoxI GGCC 3 cut(s) 182, 547, 884
ApeKI GCWGC 4 cut(s) 14, 150, 510, 841
ApoI RAATTY 3 cut(s) 413, 472, 619
Asp700I GAANNNNTTC 1 cut(s) 711
AspS9I GGNCC 1 cut(s) 339
AsuHPI GGTGA 5 cut(s) 278, 320, 826, 920, 983
AvaII GGWCC 1 cut(s) 339
BanII GRGCYC 1 cut(s) 599
BbvCI CCTCAGC 1 cut(s) 343
BbvI GCAGC 3 cut(s) 162, 497, 853
BccI CCATC 5 cut(s) 164, 263, 500, 647, 866
BcoDI GTCTC 1 cut(s) 42
BfaI CTAG 2 cut(s) 155, 452
BisI GCNGC 5 cut(s) 15, 151, 511, 550, 842
BlpI GCTNAGC 1 cut(s) 146
BlsI GCNGC 5 cut(s) 16, 152, 512, 551, 843
BmcAI AGTACT 1 cut(s) 700
Bme18I GGWCC 1 cut(s) 339
BmgBI CACGTC 1 cut(s) 976
BmgT120I GGNCC 1 cut(s) 339
BmiI GGNNCC 1 cut(s) 596
BmrI ACTGGG 1 cut(s) 33
BmsI GCATC 2 cut(s) 13, 691
BmuI ACTGGG 1 cut(s) 33
BoxI GACNNNNGTC 1 cut(s) 978
BplI GAGNNNNNCTC 2 cut(s) 906, 938
BpmI CTGGAG 1 cut(s) 723
Bpu10I CCTNAGC 1 cut(s) 343
Bpu1102I GCTNAGC 1 cut(s) 146
Bsa29I ATCGAT 1 cut(s) 657
BsaAI YACGTR 1 cut(s) 812
Bsc4I CCNNNNNNNGG 3 cut(s) 160, 295, 336
Bse118I RCCGGY 1 cut(s) 116
Bse1I ACTGG 3 cut(s) 28, 322, 706
Bse3DI GCAATG 1 cut(s) 96
BseCI ATCGAT 1 cut(s) 657
BseGI GGATG 6 cut(s) 4, 105, 175, 682, 783, 877
BseLI CCNNNNNNNGG 3 cut(s) 160, 295, 336
BseMI GCAATG 1 cut(s) 96
BseMII CTCAG 3 cut(s) 137, 217, 357
BseNI ACTGG 3 cut(s) 28, 322, 706
BseRI GAGGAG 2 cut(s) 191, 304
BseXI GCAGC 3 cut(s) 162, 497, 853
Bsh1285I CGRYCG 1 cut(s) 657
BshFI GGCC 3 cut(s) 184, 549, 886
BshVI ATCGAT 1 cut(s) 657
BsiEI CGRYCG 1 cut(s) 657
BsiSI CCGG 1 cut(s) 117
BslFI GGGAC 2 cut(s) 313, 582
BslI CCNNNNNNNGG 3 cut(s) 160, 295, 336
BsmAI GTCTC 1 cut(s) 42
BsmFI GGGAC 2 cut(s) 313, 582
BsnI GGCC 3 cut(s) 184, 549, 886
Bsp1286I GDGCHC 1 cut(s) 599
Bsp143I GATC 3 cut(s) 135, 634, 654
Bsp1720I GCTNAGC 1 cut(s) 146
BspACI CCGC 1 cut(s) 550
BspANI GGCC 3 cut(s) 184, 549, 886
BspCNI CTCAG 3 cut(s) 138, 216, 356
BspDI ATCGAT 1 cut(s) 657
BspHI TCATGA 2 cut(s) 559, 936
BspLI GGNNCC 1 cut(s) 596
BspPI GGATC 1 cut(s) 629
BspQI GCTCTTC 1 cut(s) 45
BsrDI GCAATG 1 cut(s) 96
BsrFI RCCGGY 1 cut(s) 116
BsrI ACTGG 3 cut(s) 28, 322, 706
BssAI RCCGGY 1 cut(s) 116
BssMI GATC 3 cut(s) 135, 634, 654
Bst4CI ACNGT 1 cut(s) 853
Bst6I CTCTTC 6 cut(s) 45, 207, 496, 558, 723, 864
BstBAI YACGTR 1 cut(s) 812
BstC8I GCNNGC 2 cut(s) 118, 846
BstDEI CTNAG 3 cut(s) 146, 203, 343
BstENI CCTNNNNNAGG 1 cut(s) 293
BstF5I GGATG 6 cut(s) 4, 105, 175, 682, 783, 877
BstKTI GATC 3 cut(s) 138, 637, 657
BstMAI GTCTC 1 cut(s) 42
BstMBI GATC 3 cut(s) 135, 634, 654
BstMCI CGRYCG 1 cut(s) 657
BstPAI GACNNNNGTC 1 cut(s) 978
BstV1I GCAGC 3 cut(s) 162, 497, 853
Bsu15I ATCGAT 1 cut(s) 657
BsuRI GGCC 3 cut(s) 184, 549, 886
BsuTUI ATCGAT 1 cut(s) 657
BtrI CACGTC 1 cut(s) 976
BtsCI GGATG 6 cut(s) 4, 105, 175, 682, 783, 877
Cac8I GCNNGC 2 cut(s) 118, 846
CciI TCATGA 2 cut(s) 559, 936
Cfr10I RCCGGY 1 cut(s) 116
Cfr13I GGNCC 1 cut(s) 339
ClaI ATCGAT 1 cut(s) 657
Csp6I GTAC 2 cut(s) 699, 781
CspCI CAANNNNNGTGG 2 cut(s) 907, 942
CviAII CATG 6 cut(s) 422, 428, 560, 572, 608, 937
CviQI GTAC 2 cut(s) 699, 781
DdeI CTNAG 3 cut(s) 146, 203, 343
DpnI GATC 3 cut(s) 137, 636, 656
DpnII GATC 3 cut(s) 135, 634, 654
DraI TTTAAA 1 cut(s) 435
Eam1104I CTCTTC 6 cut(s) 45, 207, 496, 558, 723, 864
EarI CTCTTC 6 cut(s) 45, 207, 496, 558, 723, 864
Eco24I GRGCYC 1 cut(s) 599
Eco47I GGWCC 1 cut(s) 339
Eco57I CTGAAG 2 cut(s) 278, 704
EcoNI CCTNNNNNAGG 1 cut(s) 293
EcoT22I ATGCAT 2 cut(s) 6, 684
EcoT38I GRGCYC 1 cut(s) 599
FaeI CATG 6 cut(s) 425, 431, 563, 575, 611, 940
FaqI GGGAC 2 cut(s) 313, 582
FatI CATG 6 cut(s) 421, 427, 559, 571, 607, 936
Fnu4HI GCNGC 5 cut(s) 15, 151, 511, 550, 842
FokI GGATG 5 cut(s) 92, 182, 669, 770, 884
FriOI GRGCYC 1 cut(s) 599
Fsp4HI GCNGC 5 cut(s) 15, 151, 511, 550, 842
FspBI CTAG 2 cut(s) 155, 452
GluI GCNGC 5 cut(s) 15, 151, 511, 550, 842
GsuI CTGGAG 1 cut(s) 723
HaeIII GGCC 3 cut(s) 184, 549, 886
HapII CCGG 1 cut(s) 117
Hin1II CATG 6 cut(s) 425, 431, 563, 575, 611, 940
HincII GTYRAC 2 cut(s) 132, 490
HindII GTYRAC 2 cut(s) 132, 490
HindIII AAGCTT 1 cut(s) 437
HinfI GANTC 4 cut(s) 62, 311, 480, 665
HpaII CCGG 1 cut(s) 117
HphI GGTGA 5 cut(s) 278, 320, 826, 920, 983
Hpy166II GTNNAC 2 cut(s) 132, 490
Hpy188I TCNGA 6 cut(s) 61, 67, 110, 169, 670, 745
Hpy188III TCNNGA 6 cut(s) 209, 560, 760, 823, 937, 989
Hpy8I GTNNAC 2 cut(s) 132, 490
HpyAV CCTTC 6 cut(s) 84, 299, 367, 585, 636, 899
HpyCH4III ACNGT 1 cut(s) 853
HpyCH4IV ACGT 2 cut(s) 811, 975
HpyCH4V TGCA 6 cut(s) 4, 17, 89, 513, 682, 767
HpyF3I CTNAG 3 cut(s) 146, 203, 343
HpySE526I ACGT 2 cut(s) 811, 975
Hsp92II CATG 6 cut(s) 425, 431, 563, 575, 611, 940
KroI GCCGGC 1 cut(s) 116
KroNI GCCGGC 1 cut(s) 118
Kzo9I GATC 3 cut(s) 135, 634, 654
LguI GCTCTTC 1 cut(s) 45
LmnI GCTCC 1 cut(s) 594
Lsp1109I GCAGC 3 cut(s) 162, 497, 853
LweI GCATC 2 cut(s) 13, 691
MaeI CTAG 2 cut(s) 155, 452
MaeII ACGT 2 cut(s) 811, 975
MaeIII GTNAC 4 cut(s) 308, 526, 853, 971
MalI GATC 3 cut(s) 137, 636, 656
MboI GATC 3 cut(s) 135, 634, 654
MfeI CAATTG 1 cut(s) 630
MhlI GDGCHC 1 cut(s) 599
MluCI AATT 7 cut(s) 413, 472, 582, 619, 630, 814, 959
MlyI GAGTC 1 cut(s) 305
MmeI TCCRAC 1 cut(s) 386
Mph1103I ATGCAT 2 cut(s) 6, 684
MroNI GCCGGC 1 cut(s) 116
MroXI GAANNNNTTC 1 cut(s) 711
MseI TTAA 4 cut(s) 363, 378, 434, 994
MslI CAYNNNNRTG 2 cut(s) 426, 576
MspA1I CMGCKG 1 cut(s) 347
MspI CCGG 1 cut(s) 117
MunI CAATTG 1 cut(s) 630
NaeI GCCGGC 1 cut(s) 118
NdeII GATC 3 cut(s) 135, 634, 654
NgoMIV GCCGGC 1 cut(s) 116
NlaIII CATG 6 cut(s) 425, 431, 563, 575, 611, 940
NlaIV GGNNCC 1 cut(s) 596
NmeAIII GCCGAG 1 cut(s) 974
NmuCI GTSAC 2 cut(s) 308, 971
NsiI ATGCAT 2 cut(s) 6, 684
PagI TCATGA 2 cut(s) 559, 936
PciSI GCTCTTC 1 cut(s) 45
PdiI GCCGGC 1 cut(s) 118
PdmI GAANNNNTTC 1 cut(s) 711
PfeI GAWTC 3 cut(s) 62, 480, 665
PkrI GCNGC 5 cut(s) 16, 152, 512, 551, 843
Ple19I CGATCG 1 cut(s) 657
PleI GAGTC 1 cut(s) 305
PpsI GAGTC 1 cut(s) 305
Ppu21I YACGTR 1 cut(s) 812
PshAI GACNNNNGTC 1 cut(s) 978
PspN4I GGNNCC 1 cut(s) 596
PspPI GGNCC 1 cut(s) 339
PvuI CGATCG 1 cut(s) 657
PvuII CAGCTG 1 cut(s) 347
RsaI GTAC 2 cut(s) 700, 782
RsaNI GTAC 2 cut(s) 699, 781
RseI CAYNNNNRTG 2 cut(s) 426, 576
SapI GCTCTTC 1 cut(s) 45
SaqAI TTAA 4 cut(s) 363, 378, 434, 994
SatI GCNGC 5 cut(s) 15, 151, 511, 550, 842
Sau3AI GATC 3 cut(s) 135, 634, 654
Sau96I GGNCC 1 cut(s) 339
ScaI AGTACT 1 cut(s) 700
SchI GAGTC 1 cut(s) 305
SduI GDGCHC 1 cut(s) 599
SfaNI GCATC 2 cut(s) 13, 691
SinI GGWCC 1 cut(s) 339
SmiMI CAYNNNNRTG 2 cut(s) 426, 576
Sse9I AATT 7 cut(s) 413, 472, 582, 619, 630, 814, 959
SsiI CCGC 1 cut(s) 550
SspMI CTAG 2 cut(s) 155, 452
TaaI ACNGT 1 cut(s) 853
TaiI ACGT 2 cut(s) 814, 978
TaqI TCGA 1 cut(s) 657
TasI AATT 7 cut(s) 413, 472, 582, 619, 630, 814, 959
TatI WGTACW 1 cut(s) 698
TauI GCSGC 1 cut(s) 552
TfiI GAWTC 3 cut(s) 62, 480, 665
Tru1I TTAA 4 cut(s) 363, 378, 434, 994
Tru9I TTAA 4 cut(s) 363, 378, 434, 994
TseFI GTSAC 2 cut(s) 308, 971
TseI GCWGC 4 cut(s) 14, 150, 510, 841
Tsp45I GTSAC 2 cut(s) 308, 971
TspDTI ATGAA 3 cut(s) 576, 663, 925
TspGWI ACGGA 1 cut(s) 776
VpaK11BI GGWCC 1 cut(s) 339
XagI CCTNNNNNAGG 1 cut(s) 293
XapI RAATTY 3 cut(s) 413, 472, 619
XmnI GAANNNNTTC 1 cut(s) 711
XspI CTAG 2 cut(s) 155, 452
ZrmI AGTACT 1 cut(s) 700
Zsp2I ATGCAT 2 cut(s) 6, 684
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.