Rorug06G0081400

B3 DNA binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0000593
Physical Location & Seq
Reverse (-)
10860878 .. 10861957
1080 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0081400.1

Sequence Viewer

Length: 1080 bp
ATGAGTCTGAACGGCAACAGCATCCGGATCCACAGACAGAATAGATCCAGATCTGGATCGACTACGCCCAGAACACCAAATCGCCGCCGCCCAGTCCCAGAACTCAGAAGCAAAGATGACACCTTAACAGCACCGCACCTCCGCTCCTCACCTGCAGAAGTCCACAAGCTGCATCGAGAACTCACAGATCGACCACAACCTGCCACCTTCCCACGGCCACGTCTGCGCCTGAGCCCCCCTCCGGAATGCGTAAAAACAACACAGACCCGTCCGACGACGACGTCCCAAGGACGCGCCGCCGGACGGAGCATCACTCTCTGTCAGGAAAACCAGGCGCGTGAGGCGCGTTCTTTTTTCTCTTTTTTTAGCTTTGAGTCGCGATTCTTTTTTTTTCATCATACCTCAGATGTTCTTTTTAATCCTTCATATGGCGGTAAGTATTTCTCCTTCACAATCACAAGTAAAGGTGTGAACTTATTCTATCCCAAGATTCCAGAGGACTTTGCAGCCATTGACATCTCAAGCAACAGATTCGAAGGGAAGGTTCCTGAATTCATTGGGGCCCTGACGGGGCTTCGTTGGCTCAATATGTCCAACAACATTCTCACTGGTTTGATCCCCTCATCCTTGGGAAAATTAAAGTTGCTTGAATCGTTGGACCTTTCACAGAACAAACTCTCAGGGCAGATCCCTCGACAACTGACACAGATTACATTCCTTGCAAAATTCGATGTCTCTCACAACAATCTCAGTGGTCCTATTCCTCGTGGAAGCCAGTTTGCTACATTTGAGAGTACTTCATATGAAGGAAACCTGGGGTTGTGTGGAGATCCATTGCCAAAGAAATGTGGCAATGAAGCTCCTCAATTGCTACCTTCAACAGAAGAAGAAAATGGTTCTGATTTTGGAAATGAATTGGATTGGATATTTGTTCTGGCAGGATCTGTGTGTGGGTTGCTAGTTGGAAGGGTTCTTGCAGTCGTCCTCATCACAAGGAAGCGTGAGTATTTCCTTGAGATTGTGGGGATACTGACCAGACTAATGAAAAAGAAAAGTGAGAAGAGGACTCGGAGAAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

359

Amino Acids

40.32

Weight (kDa)

10.04

Isoelectric Point (pI)

56.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_8 PF13855 171 - 226 3e-08 Leucine rich repeat
LRR_8 PF13855 191 - 250 8e-07 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 160
AasI GACNNNNNNGTC 1 cut(s) 280
AatII GACGTC 1 cut(s) 284
Acc36I ACCTGC 2 cut(s) 160, 208
AccBSI CCGCTC 1 cut(s) 144
AccII CGCG 4 cut(s) 294, 337, 346, 379
AccIII TCCGGA 2 cut(s) 24, 241
AciI CCGC 6 cut(s) 85, 88, 134, 142, 297, 432
AclWI GGATC 8 cut(s) 22, 35, 39, 64, 610, 682, 824, 949
AcoI YGGCCR 1 cut(s) 215
AcsI RAATTY 2 cut(s) 551, 725
AcyI GRCGYC 1 cut(s) 281
AfaI GTAC 1 cut(s) 796
AfiI CCNNNNNNNGG 5 cut(s) 213, 241, 303, 428, 570
AgsI TTSAA 2 cut(s) 650, 879
AjiI CACGTC 1 cut(s) 221
AjnI CCWGG 2 cut(s) 330, 813
AluBI AGCT 3 cut(s) 169, 369, 860
AluI AGCT 3 cut(s) 169, 369, 860
Alw26I GTCTC 1 cut(s) 739
AlwI GGATC 8 cut(s) 22, 35, 39, 64, 610, 682, 824, 949
AlwNI CAGNNNCTG 1 cut(s) 944
Aor13HI TCCGGA 2 cut(s) 24, 241
AoxI GGCC 2 cut(s) 215, 561
ApaI GGGCCC 1 cut(s) 565
ApeKI GCWGC 2 cut(s) 169, 506
ApoI RAATTY 2 cut(s) 551, 725
Asp700I GAANNNNTTC 1 cut(s) 476
AspLEI GCGC 4 cut(s) 228, 296, 337, 346
AspS9I GGNCC 4 cut(s) 561, 562, 658, 755
AsuHPI GGTGA 1 cut(s) 141
AsuII TTCGAA 1 cut(s) 534
AvaII GGWCC 2 cut(s) 658, 755
BaeGI GKGCMC 1 cut(s) 565
BamHI GGATCC 1 cut(s) 27
BanII GRGCYC 2 cut(s) 236, 565
BarI GAAGNNNNNNTAC 2 cut(s) 431, 463
BauI CACGAG 1 cut(s) 765
BbvI GCAGC 2 cut(s) 156, 518
BceAI ACGGC 2 cut(s) 28, 230
BcgI CGANNNNNNTGC 4 cut(s) 514, 548, 674, 708
BciT130I CCWGG 2 cut(s) 332, 815
BciVI GTATCC 1 cut(s) 1020
BcoDI GTCTC 1 cut(s) 739
BfaI CTAG 1 cut(s) 959
BfmI CTRYAG 1 cut(s) 153
BfuAI ACCTGC 2 cut(s) 160, 208
BfuI GTATCC 1 cut(s) 1020
BglII AGATCT 1 cut(s) 50
BisI GCNGC 5 cut(s) 85, 88, 170, 297, 507
BlsI GCNGC 5 cut(s) 86, 89, 171, 298, 508
BmcAI AGTACT 1 cut(s) 796
Bme1390I CCNGG 2 cut(s) 332, 815
Bme18I GGWCC 2 cut(s) 658, 755
BmgBI CACGTC 1 cut(s) 221
BmgT120I GGNCC 4 cut(s) 561, 562, 658, 755
BmiI GGNNCC 4 cut(s) 29, 546, 562, 563
BmrFI CCNGG 2 cut(s) 332, 815
BmrI ACTGGG 1 cut(s) 86
BmsI GCATC 3 cut(s) 30, 181, 318
BmuI ACTGGG 1 cut(s) 86
BplI GAGNNNNNCTC 4 cut(s) 223, 255, 298, 330
Bpu10I CCTNAGC 1 cut(s) 230
Bpu14I TTCGAA 1 cut(s) 534
BpuEI CTTGAG 2 cut(s) 505, 1034
BsaBI GATNNNNATC 2 cut(s) 49, 55
BsaHI GRCGYC 1 cut(s) 281
BsaJI CCNNGG 4 cut(s) 212, 286, 627, 814
BsaWI WCCGGW 2 cut(s) 24, 241
BsaXI ACNNNNNCTCC 4 cut(s) 123, 128, 153, 158
Bsc4I CCNNNNNNNGG 5 cut(s) 213, 241, 303, 428, 570
Bse1I ACTGG 3 cut(s) 92, 613, 775
Bse3DI GCAATG 2 cut(s) 833, 859
Bse8I GATNNNNATC 2 cut(s) 49, 55
BseAI TCCGGA 2 cut(s) 24, 241
BseBI CCWGG 2 cut(s) 332, 815
BseDI CCNNGG 4 cut(s) 212, 286, 627, 814
BseGI GGATG 2 cut(s) 21, 623
BseJI GATNNNNATC 2 cut(s) 49, 55
BseLI CCNNNNNNNGG 5 cut(s) 213, 241, 303, 428, 570
BseMI GCAATG 2 cut(s) 833, 859
BseMII CTCAG 5 cut(s) 118, 221, 417, 693, 763
BseNI ACTGG 3 cut(s) 92, 613, 775
BseRI GAGGAG 2 cut(s) 136, 852
BseSI GKGCMC 1 cut(s) 565
BseXI GCAGC 2 cut(s) 156, 518
Bsh1236I CGCG 4 cut(s) 294, 337, 346, 379
BshFI GGCC 2 cut(s) 217, 563
BsiSI CCGG 3 cut(s) 25, 242, 300
BslFI GGGAC 2 cut(s) 80, 268
BslI CCNNNNNNNGG 5 cut(s) 213, 241, 303, 428, 570
BsmAI GTCTC 1 cut(s) 739
BsmFI GGGAC 2 cut(s) 80, 268
BsmI GAATGC 1 cut(s) 251
BsnI GGCC 2 cut(s) 217, 563
Bsp119I TTCGAA 1 cut(s) 534
Bsp120I GGGCCC 1 cut(s) 561
Bsp1286I GDGCHC 2 cut(s) 236, 565
Bsp13I TCCGGA 2 cut(s) 24, 241
Bsp143I GATC 9 cut(s) 27, 44, 50, 56, 187, 615, 687, 829, 941
Bsp68I TCGCGA 1 cut(s) 379
BspACI CCGC 6 cut(s) 85, 88, 134, 142, 297, 432
BspANI GGCC 2 cut(s) 217, 563
BspCNI CTCAG 5 cut(s) 117, 222, 416, 692, 762
BspEI TCCGGA 2 cut(s) 24, 241
BspFNI CGCG 4 cut(s) 294, 337, 346, 379
BspLI GGNNCC 4 cut(s) 29, 546, 562, 563
BspMAI CTGCAG 1 cut(s) 157
BspMI ACCTGC 2 cut(s) 160, 208
BspPI GGATC 8 cut(s) 22, 35, 39, 64, 610, 682, 824, 949
BspT104I TTCGAA 1 cut(s) 534
BsrBI CCGCTC 1 cut(s) 144
BsrDI GCAATG 2 cut(s) 833, 859
BsrI ACTGG 3 cut(s) 92, 613, 775
BssECI CCNNGG 4 cut(s) 212, 286, 627, 814
BssMI GATC 9 cut(s) 27, 44, 50, 56, 187, 615, 687, 829, 941
BssNI GRCGYC 1 cut(s) 281
BssSI CACGAG 1 cut(s) 765
BssT1I CCWWGG 2 cut(s) 286, 627
Bst2BI CACGAG 1 cut(s) 765
Bst2UI CCWGG 2 cut(s) 332, 815
Bst6I CTCTTC 1 cut(s) 1055
BstACI GRCGYC 1 cut(s) 281
BstBI TTCGAA 1 cut(s) 534
BstDEI CTNAG 5 cut(s) 104, 230, 403, 679, 749
BstDSI CCRYGG 1 cut(s) 212
BstF5I GGATG 2 cut(s) 21, 623
BstFNI CGCG 4 cut(s) 294, 337, 346, 379
BstHHI GCGC 4 cut(s) 228, 296, 337, 346
BstKTI GATC 9 cut(s) 30, 47, 53, 59, 190, 618, 690, 832, 944
BstMAI GTCTC 1 cut(s) 739
BstMBI GATC 9 cut(s) 27, 44, 50, 56, 187, 615, 687, 829, 941
BstMWI GCNNNNNNNGC 4 cut(s) 223, 341, 343, 580
BstNI CCWGG 2 cut(s) 332, 815
BstSCI CCNGG 2 cut(s) 330, 813
BstSFI CTRYAG 1 cut(s) 153
BstSLI GKGCMC 1 cut(s) 565
BstUI CGCG 4 cut(s) 294, 337, 346, 379
BstV1I GCAGC 2 cut(s) 156, 518
BstX2I RGATCY 6 cut(s) 27, 44, 50, 687, 829, 941
BstYI RGATCY 6 cut(s) 27, 44, 50, 687, 829, 941
BsuI GTATCC 1 cut(s) 1020
BsuRI GGCC 2 cut(s) 217, 563
BtgI CCRYGG 1 cut(s) 212
BtrI CACGTC 1 cut(s) 221
BtsCI GGATG 2 cut(s) 21, 623
BtsIMutI CAGTG 2 cut(s) 606, 757
BtuMI TCGCGA 1 cut(s) 379
BveI ACCTGC 2 cut(s) 160, 208
CaiI CAGNNNCTG 1 cut(s) 944
CfoI GCGC 4 cut(s) 228, 296, 337, 346
Cfr13I GGNCC 4 cut(s) 561, 562, 658, 755
CseI GACGC 1 cut(s) 300
Csp6I GTAC 1 cut(s) 795
CspCI CAANNNNNGTGG 2 cut(s) 733, 768
CviQI GTAC 1 cut(s) 795
DdeI CTNAG 5 cut(s) 104, 230, 403, 679, 749
DpnI GATC 9 cut(s) 29, 46, 52, 58, 189, 617, 689, 831, 943
DpnII GATC 9 cut(s) 27, 44, 50, 56, 187, 615, 687, 829, 941
DrdI GACNNNNNNGTC 1 cut(s) 280
DseDI GACNNNNNNGTC 1 cut(s) 280
EaeI YGGCCR 1 cut(s) 215
Eam1104I CTCTTC 1 cut(s) 1055
EarI CTCTTC 1 cut(s) 1055
Eco130I CCWWGG 2 cut(s) 286, 627
Eco24I GRGCYC 2 cut(s) 236, 565
Eco47I GGWCC 2 cut(s) 658, 755
EcoO109I RGGNCCY 2 cut(s) 561, 562
EcoRI GAATTC 1 cut(s) 551
EcoRII CCWGG 2 cut(s) 330, 813
EcoT14I CCWWGG 2 cut(s) 286, 627
EcoT38I GRGCYC 2 cut(s) 236, 565
ErhI CCWWGG 2 cut(s) 286, 627
FaiI YATR 6 cut(s) 399, 427, 429, 590, 802, 804
FaqI GGGAC 2 cut(s) 80, 268
FauNDI CATATG 2 cut(s) 427, 802
Fnu4HI GCNGC 5 cut(s) 85, 88, 170, 297, 507
FokI GGATG 2 cut(s) 8, 610
FriOI GRGCYC 2 cut(s) 236, 565
Fsp4HI GCNGC 5 cut(s) 85, 88, 170, 297, 507
FspBI CTAG 1 cut(s) 959
GlaI GCGC 4 cut(s) 227, 295, 336, 345
GluI GCNGC 5 cut(s) 85, 88, 170, 297, 507
HaeIII GGCC 2 cut(s) 217, 563
HapII CCGG 3 cut(s) 25, 242, 300
HgaI GACGC 1 cut(s) 300
HhaI GCGC 4 cut(s) 228, 296, 337, 346
Hin1I GRCGYC 1 cut(s) 281
Hin6I GCGC 4 cut(s) 226, 294, 335, 344
HinP1I GCGC 4 cut(s) 226, 294, 335, 344
HinfI GANTC 7 cut(s) 4, 374, 381, 490, 531, 650, 1066
HpaII CCGG 3 cut(s) 25, 242, 300
HphI GGTGA 1 cut(s) 141
Hpy166II GTNNAC 2 cut(s) 163, 472
Hpy188I TCNGA 6 cut(s) 9, 107, 273, 406, 901, 1071
Hpy188III TCNNGA 9 cut(s) 25, 48, 54, 176, 242, 323, 378, 494, 548
Hpy8I GTNNAC 2 cut(s) 163, 472
Hpy99I CGWCG 3 cut(s) 277, 280, 283
HpyAV CCTTC 8 cut(s) 217, 432, 457, 530, 535, 800, 885, 960
HpyCH4IV ACGT 2 cut(s) 220, 281
HpyCH4V TGCA 5 cut(s) 155, 172, 506, 722, 977
HpyF10VI GCNNNNNNNGC 4 cut(s) 223, 341, 343, 580
HpyF3I CTNAG 5 cut(s) 104, 230, 403, 679, 749
HpySE526I ACGT 2 cut(s) 220, 281
Hsp92I GRCGYC 1 cut(s) 281
HspAI GCGC 4 cut(s) 226, 294, 335, 344
Kpn2I TCCGGA 2 cut(s) 24, 241
Kzo9I GATC 9 cut(s) 27, 44, 50, 56, 187, 615, 687, 829, 941
LmnI GCTCC 3 cut(s) 149, 306, 865
Lsp1109I GCAGC 2 cut(s) 156, 518
LweI GCATC 3 cut(s) 30, 181, 318
MaeI CTAG 1 cut(s) 959
MaeII ACGT 2 cut(s) 220, 281
MalI GATC 9 cut(s) 29, 46, 52, 58, 189, 617, 689, 831, 943
MbiI CCGCTC 1 cut(s) 144
MboI GATC 9 cut(s) 27, 44, 50, 56, 187, 615, 687, 829, 941
MboII GAAGA 3 cut(s) 896, 899, 1072
MfeI CAATTG 1 cut(s) 866
MflI RGATCY 6 cut(s) 27, 44, 50, 687, 829, 941
MhlI GDGCHC 2 cut(s) 236, 565
MluCI AATT 6 cut(s) 551, 635, 725, 866, 914, 1075
MlyI GAGTC 3 cut(s) 13, 383, 1060
MmeI TCCRAC 4 cut(s) 296, 618, 636, 943
MroI TCCGGA 2 cut(s) 24, 241
MroXI GAANNNNTTC 1 cut(s) 476
MseI TTAA 3 cut(s) 125, 417, 638
MspI CCGG 3 cut(s) 25, 242, 300
MspR9I CCNGG 2 cut(s) 332, 815
MunI CAATTG 1 cut(s) 866
Mva1269I GAATGC 1 cut(s) 251
MvaI CCWGG 2 cut(s) 332, 815
MvnI CGCG 4 cut(s) 294, 337, 346, 379
MwoI GCNNNNNNNGC 4 cut(s) 223, 341, 343, 580
NdeI CATATG 2 cut(s) 427, 802
NdeII GATC 9 cut(s) 27, 44, 50, 56, 187, 615, 687, 829, 941
NlaIV GGNNCC 4 cut(s) 29, 546, 562, 563
NruI TCGCGA 1 cut(s) 379
NspV TTCGAA 1 cut(s) 534
PaqCI CACCTGC 1 cut(s) 160
PctI GAATGC 1 cut(s) 251
PdmI GAANNNNTTC 1 cut(s) 476
PfeI GAWTC 4 cut(s) 381, 490, 531, 650
PflFI GACNNNGTC 1 cut(s) 280
PkrI GCNGC 5 cut(s) 86, 89, 171, 298, 508
PleI GAGTC 3 cut(s) 12, 382, 1060
PpsI GAGTC 3 cut(s) 12, 382, 1060
Psp6I CCWGG 2 cut(s) 330, 813
PspGI CCWGG 2 cut(s) 330, 813
PspN4I GGNNCC 4 cut(s) 29, 546, 562, 563
PspOMI GGGCCC 1 cut(s) 561
PspPI GGNCC 4 cut(s) 561, 562, 658, 755
PstI CTGCAG 1 cut(s) 157
PstNI CAGNNNCTG 1 cut(s) 944
PsuI RGATCY 6 cut(s) 27, 44, 50, 687, 829, 941
PsyI GACNNNGTC 1 cut(s) 280
RruI TCGCGA 1 cut(s) 379
RsaI GTAC 1 cut(s) 796
RsaNI GTAC 1 cut(s) 795
SaqAI TTAA 3 cut(s) 125, 417, 638
SatI GCNGC 5 cut(s) 85, 88, 170, 297, 507
Sau3AI GATC 9 cut(s) 27, 44, 50, 56, 187, 615, 687, 829, 941
Sau96I GGNCC 4 cut(s) 561, 562, 658, 755
ScaI AGTACT 1 cut(s) 796
SchI GAGTC 3 cut(s) 13, 383, 1060
ScrFI CCNGG 2 cut(s) 332, 815
SduI GDGCHC 2 cut(s) 236, 565
SfaNI GCATC 3 cut(s) 30, 181, 318
SfcI CTRYAG 1 cut(s) 153
SfuI TTCGAA 1 cut(s) 534
SinI GGWCC 2 cut(s) 658, 755
SmlI CTYRAG 2 cut(s) 520, 1013
SmoI CTYRAG 2 cut(s) 520, 1013
Sse9I AATT 6 cut(s) 551, 635, 725, 866, 914, 1075
SsiI CCGC 6 cut(s) 85, 88, 134, 142, 297, 432
SspMI CTAG 1 cut(s) 959
StyD4I CCNGG 2 cut(s) 330, 813
StyI CCWWGG 2 cut(s) 286, 627
TaiI ACGT 2 cut(s) 223, 284
TaqI TCGA 6 cut(s) 59, 175, 190, 534, 694, 729
TasI AATT 6 cut(s) 551, 635, 725, 866, 914, 1075
TatI WGTACW 1 cut(s) 794
TauI GCSGC 3 cut(s) 87, 90, 299
TfiI GAWTC 4 cut(s) 381, 490, 531, 650
Tru1I TTAA 3 cut(s) 125, 417, 638
Tru9I TTAA 3 cut(s) 125, 417, 638
TscAI CASTG 2 cut(s) 613, 757
TseI GCWGC 2 cut(s) 169, 506
TspDTI ATGAA 8 cut(s) 383, 414, 544, 789, 819, 870, 927, 1058
TspGWI ACGGA 1 cut(s) 319
TspRI CASTG 2 cut(s) 613, 757
Tth111I GACNNNGTC 1 cut(s) 280
VpaK11BI GGWCC 2 cut(s) 658, 755
XapI RAATTY 2 cut(s) 551, 725
XmnI GAANNNNTTC 1 cut(s) 476
XspI CTAG 1 cut(s) 959
ZraI GACGTC 1 cut(s) 282
ZrmI AGTACT 1 cut(s) 796
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.