RLG00000017233

B3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0000593
Physical Location & Seq
Reverse (-)
14400327 .. 14402943
2617 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000017233

Sequence Viewer

Length: 1476 bp
ATGGCAACAAGAAGAAAACCCTCTTTCTTCAAGGTTCTTATAGGGGATCGCTTCTGTAACAGATTGAAAATTCCCGGAGCTTTTCTTGAGCATTTTGATGGGAGAGTACCTGAACAGTGCCATCTTAGAATTGGCACTCAAACTTGGCTTGTTGATGTTGAAATGGTCAATCAGAAGTTCTATTTCCAAAATGGTTGGAAGGAATTTGTGCTTGAGAATGGTTTAAGGGGTTGCGAGTTTCTAGTTTTTTTCTATAATGGAAATTCGGAGTTCTATGTCCACATATATGGGAAGAATGCTTGCAGAAAGGACTTTGCAGATGCCGCTGCTGGAGTAAGTGGCACACCGAAATCTATACATGTTGGTAGCTCCAGAGGGATGGAAGAAGCTGATAGTAATGACTCATCTGTTCAGATCTTGGATGACTTTCCAACCTGCTTAAGAAGAGCGGGGGAGAAATCTTCATTACCAAGTCATCAGCAGCACAAGAAAGACAGTACAAGCTGGATTGGTAAAGAGACACATGGTCCCAAACCAGGAATTACAAGAAAAGAAAATGATTTTCCAGCGGAACAATATGTGGGAGGGACTTCTAGCTCGAAACCAAAACATGAGGTTCTTGGGAGGATGCACACTGATGACGATGATGATGACAAAGAAGAGGAAGAGAGTGAGGAGGATGATGATGATTATGAGGAAGATGAAGAGAGTGGGGAGGATGAAAATGAAGCTGACAGTGAAGATGATGATGAATTTGAAAATGAAGCTGACAGTGAAGACAAAGATGGGTATGAAGATGAAGAAGAGGAAGAAGAAAGCGACGGAGATGAGGGAAAAAGTGATGATTCTATTGAAATTCTGGACGAGTTTCCACGACGCTCAAAAGCAAAAATAATGAGGAAATCATCAGGGCTTCAGAAGAGAAAGATAAACAGTTATAAAGCTAAAGCAAAAAGAAGCATAACATCTGCTAGTGGCTCGTCTAGCGATCAAAAAATCACAGGATGGAAATATAATGTTTTAAGGAAAATGCCTGGATTGAATGGAATCGGAGAACTTATAGCTCGTCACAGAGCTAAAGATTTCATATCCAAATCTGACAAGCTTGATTCTTGTTTGATTGCCATGTCTCCTTCGTATATTAACGGATCTTGTGTGCATTTTCCACGTACTTTTGTCTACAGACATCTTGGAAGGCATTGTAGTCGCATCAAACTTCGGGTTAACAAGAGGAGGTGGCGTCTTGGTATCTATCATGTCCATAAAACAGTTAGATTACAAGCTGGATGGATTCAATTTAGGGATGACAATAATCTGAAAGTGGGTGATGTGTGCGTCTTTGAGTTGACTGACAGCAATGTACTTGACGTTACAATTTTCCGCGCCGCAGAAGCTGCAAATTGCACCTTGCCAGGGGGAAGACAAACGACTGGGGTTCAATTCAAGTCGAGAAAAGGAGAAGAGAAAGCTGGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

492

Amino Acids

55.71

Weight (kDa)

5.43

Isoelectric Point (pI)

54.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 9 - 98 9.4e-11 B3 DNA binding domain
B3 PF02362 376 - 462 1.3e-10 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 939
Acc36I ACCTGC 1 cut(s) 443
AccBSI CCGCTC 1 cut(s) 449
AccI GTMKAC 1 cut(s) 1179
AccII CGCG 1 cut(s) 1383
AciI CCGC 5 cut(s) 324, 449, 569, 1381, 1386
AclWI GGATC 2 cut(s) 54, 1156
AcsI RAATTY 5 cut(s) 69, 203, 262, 752, 855
AcuI CTGAAG 1 cut(s) 899
AcyI GRCGYC 1 cut(s) 1240
AfaI GTAC 4 cut(s) 108, 499, 1171, 1362
AfiI CCNNNNNNNGG 2 cut(s) 536, 1413
AflII CTTAAG 1 cut(s) 439
AflIII ACRYGT 1 cut(s) 358
AgsI TTSAA 9 cut(s) 31, 67, 161, 758, 854, 1042, 1295, 1439, 1444
AhdI GACNNNNNGTC 1 cut(s) 525
AjnI CCWGG 3 cut(s) 535, 1033, 1411
Alw26I GTCTC 2 cut(s) 512, 1134
AlwI GGATC 2 cut(s) 54, 1156
AlwNI CAGNNNCTG 1 cut(s) 1394
ApeKI GCWGC 3 cut(s) 326, 481, 1394
ApoI RAATTY 5 cut(s) 69, 203, 262, 752, 855
AspLEI GCGC 1 cut(s) 1385
AspS9I GGNCC 1 cut(s) 527
AsuC2I CCSGG 1 cut(s) 75
AsuHPI GGTGA 1 cut(s) 1337
AvaII GGWCC 1 cut(s) 527
BbsI GAAGAC 2 cut(s) 783, 1426
BbvI GCAGC 3 cut(s) 313, 493, 1381
BccI CCATC 6 cut(s) 92, 129, 373, 779, 999, 1281
BcgI CGANNNNNNTGC 2 cut(s) 1187, 1221
BciT130I CCWGG 3 cut(s) 537, 1035, 1413
BcnI CCSGG 1 cut(s) 75
BcoDI GTCTC 2 cut(s) 512, 1134
BfaI CTAG 4 cut(s) 242, 594, 972, 984
BfmI CTRYAG 1 cut(s) 1180
BfrI CTTAAG 1 cut(s) 439
BfuAI ACCTGC 1 cut(s) 443
BglII AGATCT 1 cut(s) 414
BisI GCNGC 5 cut(s) 324, 327, 482, 1386, 1395
BlsI GCNGC 5 cut(s) 325, 328, 483, 1387, 1396
Bme1390I CCNGG 4 cut(s) 75, 537, 1035, 1413
Bme18I GGWCC 1 cut(s) 527
BmeRI GACNNNNNGTC 1 cut(s) 525
BmgT120I GGNCC 1 cut(s) 527
BmiI GGNNCC 1 cut(s) 529
BmrFI CCNGG 4 cut(s) 75, 537, 1035, 1413
BmrI ACTGGG 1 cut(s) 1440
BmsI GCATC 3 cut(s) 310, 618, 1218
BmuI ACTGGG 1 cut(s) 1440
BpiI GAAGAC 2 cut(s) 783, 1426
BpmI CTGGAG 2 cut(s) 351, 355
BpuEI CTTGAG 2 cut(s) 107, 233
BpuMI CCSGG 1 cut(s) 75
BsaAI YACGTR 1 cut(s) 1169
BsaHI GRCGYC 1 cut(s) 1240
BsaJI CCNNGG 1 cut(s) 1412
BsaXI ACNNNNNCTCC 2 cut(s) 1225, 1255
Bsc4I CCNNNNNNNGG 2 cut(s) 536, 1413
Bse1I ACTGG 1 cut(s) 1435
Bse3DI GCAATG 1 cut(s) 1363
BseBI CCWGG 3 cut(s) 537, 1035, 1413
BseDI CCNNGG 1 cut(s) 1412
BseGI GGATG 8 cut(s) 384, 427, 633, 685, 724, 1010, 1292, 1309
BseLI CCNNNNNNNGG 2 cut(s) 536, 1413
BseMI GCAATG 1 cut(s) 1363
BseNI ACTGG 1 cut(s) 1435
BseRI GAGGAG 2 cut(s) 689, 1246
BseXI GCAGC 3 cut(s) 313, 493, 1381
Bsh1236I CGCG 1 cut(s) 1383
BsiSI CCGG 1 cut(s) 75
BslFI GGGAC 2 cut(s) 513, 601
BslI CCNNNNNNNGG 2 cut(s) 536, 1413
BsmAI GTCTC 2 cut(s) 512, 1134
BsmFI GGGAC 2 cut(s) 513, 601
BsmI GAATGC 1 cut(s) 301
Bsp143I GATC 4 cut(s) 46, 414, 988, 1148
BspACI CCGC 5 cut(s) 324, 449, 569, 1381, 1386
BspFNI CGCG 1 cut(s) 1383
BspLI GGNNCC 1 cut(s) 529
BspMI ACCTGC 1 cut(s) 443
BspPI GGATC 2 cut(s) 54, 1156
BspQI GCTCTTC 1 cut(s) 439
BspTI CTTAAG 1 cut(s) 439
BsrBI CCGCTC 1 cut(s) 449
BsrDI GCAATG 1 cut(s) 1363
BsrI ACTGG 1 cut(s) 1435
BssECI CCNNGG 1 cut(s) 1412
BssMI GATC 4 cut(s) 46, 414, 988, 1148
BssNI GRCGYC 1 cut(s) 1240
Bst2UI CCWGG 3 cut(s) 537, 1035, 1413
Bst4CI ACNGT 6 cut(s) 117, 497, 737, 773, 935, 1270
Bst6I CTCTTC 7 cut(s) 439, 654, 660, 699, 798, 914, 1455
BstACI GRCGYC 1 cut(s) 1240
BstAFI CTTAAG 1 cut(s) 439
BstAPI GCANNNNNTGC 1 cut(s) 1394
BstBAI YACGTR 1 cut(s) 1169
BstC8I GCNNGC 1 cut(s) 301
BstDEI CTNAG 1 cut(s) 125
BstF5I GGATG 8 cut(s) 384, 427, 633, 685, 724, 1010, 1292, 1309
BstFNI CGCG 1 cut(s) 1383
BstHHI GCGC 1 cut(s) 1385
BstKTI GATC 4 cut(s) 49, 417, 991, 1151
BstMAI GTCTC 2 cut(s) 512, 1134
BstMBI GATC 4 cut(s) 46, 414, 988, 1148
BstMWI GCNNNNNNNGC 4 cut(s) 323, 984, 1391, 1394
BstNI CCWGG 3 cut(s) 537, 1035, 1413
BstNSI RCATGY 1 cut(s) 362
BstSCI CCNGG 4 cut(s) 73, 535, 1033, 1411
BstSFI CTRYAG 1 cut(s) 1180
BstUI CGCG 1 cut(s) 1383
BstV1I GCAGC 3 cut(s) 313, 493, 1381
BstV2I GAAGAC 2 cut(s) 783, 1426
BstX2I RGATCY 2 cut(s) 414, 1148
BstXI CCANNNNNNTGG 2 cut(s) 287, 379
BstYI RGATCY 2 cut(s) 414, 1148
BtsCI GGATG 8 cut(s) 384, 427, 633, 685, 724, 1010, 1292, 1309
BtsIMutI CAGTG 4 cut(s) 122, 633, 742, 778
BveI ACCTGC 1 cut(s) 443
Cac8I GCNNGC 1 cut(s) 301
CaiI CAGNNNCTG 1 cut(s) 1394
CfoI GCGC 1 cut(s) 1385
Cfr13I GGNCC 1 cut(s) 527
CseI GACGC 3 cut(s) 885, 1229, 1324
Csp6I GTAC 4 cut(s) 107, 498, 1170, 1361
CviAII CATG 5 cut(s) 359, 524, 611, 1126, 1256
CviQI GTAC 4 cut(s) 107, 498, 1170, 1361
DdeI CTNAG 1 cut(s) 125
DpnI GATC 4 cut(s) 48, 416, 990, 1150
DpnII GATC 4 cut(s) 46, 414, 988, 1148
DriI GACNNNNNGTC 1 cut(s) 525
Eam1104I CTCTTC 7 cut(s) 439, 654, 660, 699, 798, 914, 1455
Eam1105I GACNNNNNGTC 1 cut(s) 525
EarI CTCTTC 7 cut(s) 439, 654, 660, 699, 798, 914, 1455
Eco47I GGWCC 1 cut(s) 527
Eco57I CTGAAG 1 cut(s) 899
EcoRII CCWGG 3 cut(s) 535, 1033, 1411
FaeI CATG 5 cut(s) 362, 527, 614, 1129, 1259
FaqI GGGAC 2 cut(s) 513, 601
FatI CATG 5 cut(s) 358, 523, 610, 1125, 1255
FauI CCCGC 1 cut(s) 442
FblI GTMKAC 1 cut(s) 1179
Fnu4HI GCNGC 5 cut(s) 324, 327, 482, 1386, 1395
FokI GGATG 8 cut(s) 391, 434, 640, 692, 731, 1017, 1299, 1316
Fsp4HI GCNGC 5 cut(s) 324, 327, 482, 1386, 1395
FspBI CTAG 4 cut(s) 242, 594, 972, 984
GlaI GCGC 1 cut(s) 1384
GluI GCNGC 5 cut(s) 324, 327, 482, 1386, 1395
GsuI CTGGAG 2 cut(s) 351, 355
HapII CCGG 1 cut(s) 75
HgaI GACGC 3 cut(s) 885, 1229, 1324
HhaI GCGC 1 cut(s) 1385
Hin1I GRCGYC 1 cut(s) 1240
Hin1II CATG 5 cut(s) 362, 527, 614, 1129, 1259
Hin6I GCGC 1 cut(s) 1383
HinP1I GCGC 1 cut(s) 1383
HincII GTYRAC 2 cut(s) 1225, 1347
HindII GTYRAC 2 cut(s) 1225, 1347
HindIII AAGCTT 1 cut(s) 1103
HinfI GANTC 5 cut(s) 401, 845, 1047, 1109, 1291
HpaI GTTAAC 1 cut(s) 1225
HpaII CCGG 1 cut(s) 75
HphI GGTGA 1 cut(s) 1337
Hpy166II GTNNAC 4 cut(s) 280, 1180, 1225, 1347
Hpy188I TCNGA 7 cut(s) 174, 268, 414, 918, 1052, 1099, 1317
Hpy188III TCNNGA 4 cut(s) 86, 372, 860, 1449
Hpy8I GTNNAC 4 cut(s) 280, 1180, 1225, 1347
Hpy99I CGWCG 2 cut(s) 824, 879
HpyAV CCTTC 3 cut(s) 193, 1143, 1188
HpyCH4III ACNGT 6 cut(s) 117, 497, 737, 773, 935, 1270
HpyCH4IV ACGT 2 cut(s) 1168, 1368
HpyCH4V TGCA 6 cut(s) 303, 317, 631, 1159, 1397, 1404
HpyF10VI GCNNNNNNNGC 4 cut(s) 323, 984, 1391, 1394
HpyF3I CTNAG 1 cut(s) 125
HpySE526I ACGT 2 cut(s) 1168, 1368
Hsp92I GRCGYC 1 cut(s) 1240
Hsp92II CATG 5 cut(s) 362, 527, 614, 1129, 1259
HspAI GCGC 1 cut(s) 1383
KspAI GTTAAC 1 cut(s) 1225
Kzo9I GATC 4 cut(s) 46, 414, 988, 1148
LguI GCTCTTC 1 cut(s) 439
LmnI GCTCC 2 cut(s) 77, 374
Lsp1109I GCAGC 3 cut(s) 313, 493, 1381
LweI GCATC 3 cut(s) 310, 618, 1218
MaeI CTAG 4 cut(s) 242, 594, 972, 984
MaeII ACGT 2 cut(s) 1168, 1368
MaeIII GTNAC 3 cut(s) 56, 1067, 1369
MalI GATC 4 cut(s) 48, 416, 990, 1150
MbiI CCGCTC 1 cut(s) 449
MboI GATC 4 cut(s) 46, 414, 988, 1148
MflI RGATCY 2 cut(s) 414, 1148
MlyI GAGTC 1 cut(s) 395
MmeI TCCRAC 2 cut(s) 176, 455
MseI TTAA 5 cut(s) 224, 440, 1022, 1143, 1224
MslI CAYNNNNRTG 3 cut(s) 96, 285, 636
MspA1I CMGCKG 2 cut(s) 326, 569
MspCI CTTAAG 1 cut(s) 439
MspI CCGG 1 cut(s) 75
MspR9I CCNGG 4 cut(s) 75, 537, 1035, 1413
Mva1269I GAATGC 1 cut(s) 301
MvaI CCWGG 3 cut(s) 537, 1035, 1413
MvnI CGCG 1 cut(s) 1383
MwoI GCNNNNNNNGC 4 cut(s) 323, 984, 1391, 1394
NciI CCSGG 1 cut(s) 75
NdeII GATC 4 cut(s) 46, 414, 988, 1148
NlaIII CATG 5 cut(s) 362, 527, 614, 1129, 1259
NlaIV GGNNCC 1 cut(s) 529
NmuCI GTSAC 1 cut(s) 1067
NspI RCATGY 1 cut(s) 362
PciI ACATGT 1 cut(s) 358
PciSI GCTCTTC 1 cut(s) 439
PctI GAATGC 1 cut(s) 301
PfeI GAWTC 4 cut(s) 845, 1047, 1109, 1291
PfoI TCCNGGA 1 cut(s) 73
PkrI GCNGC 5 cut(s) 325, 328, 483, 1387, 1396
PleI GAGTC 1 cut(s) 395
PpsI GAGTC 1 cut(s) 395
Ppu21I YACGTR 1 cut(s) 1169
PscI ACATGT 1 cut(s) 358
PsiI TTATAA 1 cut(s) 939
Psp6I CCWGG 3 cut(s) 535, 1033, 1411
PspGI CCWGG 3 cut(s) 535, 1033, 1411
PspN4I GGNNCC 1 cut(s) 529
PspPI GGNCC 1 cut(s) 527
PstNI CAGNNNCTG 1 cut(s) 1394
PsuI RGATCY 2 cut(s) 414, 1148
RsaI GTAC 4 cut(s) 108, 499, 1171, 1362
RsaNI GTAC 4 cut(s) 107, 498, 1170, 1361
RseI CAYNNNNRTG 3 cut(s) 96, 285, 636
SapI GCTCTTC 1 cut(s) 439
SaqAI TTAA 5 cut(s) 224, 440, 1022, 1143, 1224
SatI GCNGC 5 cut(s) 324, 327, 482, 1386, 1395
Sau3AI GATC 4 cut(s) 46, 414, 988, 1148
Sau96I GGNCC 1 cut(s) 527
SchI GAGTC 1 cut(s) 395
ScrFI CCNGG 4 cut(s) 75, 537, 1035, 1413
SfaNI GCATC 3 cut(s) 310, 618, 1218
SfcI CTRYAG 1 cut(s) 1180
SinI GGWCC 1 cut(s) 527
SmiMI CAYNNNNRTG 3 cut(s) 96, 285, 636
SmlI CTYRAG 3 cut(s) 86, 212, 439
SmoI CTYRAG 3 cut(s) 86, 212, 439
SsiI CCGC 5 cut(s) 324, 449, 569, 1381, 1386
SspMI CTAG 4 cut(s) 242, 594, 972, 984
StyD4I CCNGG 4 cut(s) 73, 535, 1033, 1411
TaaI ACNGT 6 cut(s) 117, 497, 737, 773, 935, 1270
TaiI ACGT 2 cut(s) 1171, 1371
TaqI TCGA 2 cut(s) 599, 1448
TatI WGTACW 2 cut(s) 497, 1360
TauI GCSGC 2 cut(s) 326, 1388
TfiI GAWTC 4 cut(s) 845, 1047, 1109, 1291
Tru1I TTAA 5 cut(s) 224, 440, 1022, 1143, 1224
Tru9I TTAA 5 cut(s) 224, 440, 1022, 1143, 1224
TscAI CASTG 4 cut(s) 122, 640, 742, 778
TseFI GTSAC 1 cut(s) 1067
TseI GCWGC 3 cut(s) 326, 481, 1394
Tsp45I GTSAC 1 cut(s) 1067
TspDTI ATGAA 9 cut(s) 453, 717, 735, 741, 765, 777, 807, 813, 1075
TspGWI ACGGA 2 cut(s) 837, 1161
TspRI CASTG 4 cut(s) 122, 640, 742, 778
Vha464I CTTAAG 1 cut(s) 439
VpaK11BI GGWCC 1 cut(s) 527
XapI RAATTY 5 cut(s) 69, 203, 262, 752, 855
XceI RCATGY 1 cut(s) 362
XcmI CCANNNNNNNNNTGG 1 cut(s) 128
XmiI GTMKAC 1 cut(s) 1179
XspI CTAG 4 cut(s) 242, 594, 972, 984
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.