FvH4_1g14630

B3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0000593
Physical Location & Seq
Reverse (-)
8190936 .. 8194317
3382 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g14630.t1

Sequence Viewer

Length: 1266 bp
ATGGCAACAGAAAGAAACCAATCTTTTATCAGGGTTCTTCAGGGTGATCGGTTCTCTTTCAAGTTGAAAATCCCTAGCGCATTTCTCCAGTGTTTTAATGGCTCTGTACCTTCCCAGTGCTGTCTTCGAACCCGAGGTAAGGGTTGGATTGTTTATGTGGAGAAGGTTAATAACAAGTTCTATTTCCAACTGGGTTGGAATGATTTTGTGCAAGACAATGGCTTAGTGTGTGGTGAACGTCTACTCTTCGATTATGCTGGAAACTCACAATTCCATGTGGAAGTATTTGGAATAAATACCTGCAAAAAGCTGTGTGTGCCTGAGATGGAAGAAGCTAATGAACATCAGGATGAGATATCTCTTGATATCTTGGATGACTTTCCACCTTGTCCAAGGAAAATACAGGACAAATCTTTGCTTTCAAGTCATCTGCCTCACAAGAGAAACAAAACAAGTTCTAGTGGTAAAGCAGATATCACCCTCAGCTTCCCAAAAAGGTTTCCACATCTTGAGAAGTCAGCAATGAAAAAGAAAGATGCACATTGGTTCAAATCAGAAATGATAAACATTTCTGATAATGATTCTTGTGATCATGATGAGAAAGAGTATGAGGATGATGTTCATAATGAGGAGGATGAAGACGAAAATGAAGATGGAGATGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAACTGAAGATGAAGAGATGGAGGAGGAAGATGATGATTCTGTTGAAACCCACAACGAGTCTTCACGATGCTCAAGAAAAGTGGGGAAATTAGCATCGATTCTTCACCAGAGAAAGAGAAAAAGTTCTAATGCGAAAGCAAAAACCAAAGTCAATGCTGCTGGTGGTTTGTCTGGTGAGGAAAAGATTCTAGGAGCTATAGCTCTACAAAGAGCTAATATCTTCAAATCTAATTCTGTCGACCCTTGTTTCGTGGTTTCCTTGTCTGCCACATATGCTGGTGGAAGTTTTCTTGCTGTGCCATGCAGTTTTGCCGAGAAACATCTTAAGTATCGGCAGCCTGGCCACATCACCCTTAGGGTTGAGAAGAGATTTTGGTATGTCAAGGTCAATTATCTTAGAAATATAACAAGATTACAGGCTGGCTGGACTAAATTTGCACTTGACAATAATTTGAAGACTGGCGATGCTTGTGTTTTTGTATTGACCAATGCCATTGAATTTGAATATGATGTTGCAATTTTCCACATTACAGATGCTGTTCGCACCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

422

Amino Acids

48.25

Weight (kDa)

4.94

Isoelectric Point (pI)

61.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 19 - 96 6.2e-10 B3 DNA binding domain
B3 PF02362 329 - 413 1.7e-12 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 308
AccI GTMKAC 2 cut(s) 241, 954
AcoI YGGCCR 1 cut(s) 1057
AcsI RAATTY 2 cut(s) 1148, 1214
AcuI CTGAAG 2 cut(s) 23, 741
AfaI GTAC 1 cut(s) 108
AfiI CCNNNNNNNGG 1 cut(s) 139
AflII CTTAAG 1 cut(s) 1040
AgsI TTSAA 9 cut(s) 61, 67, 423, 550, 761, 940, 1171, 1214, 1220
AjnI CCWGG 1 cut(s) 1054
AluBI AGCT 6 cut(s) 310, 335, 486, 911, 917, 929
AluI AGCT 6 cut(s) 310, 335, 486, 911, 917, 929
AlwNI CAGNNNCTG 1 cut(s) 1253
Ama87I CYCGRG 1 cut(s) 132
AoxI GGCC 1 cut(s) 1057
ApeKI GCWGC 2 cut(s) 872, 1051
ApoI RAATTY 2 cut(s) 1148, 1214
ArsI GACNNNNNNTTYG 2 cut(s) 947, 979
Asp700I GAANNNNTTC 2 cut(s) 838, 900
AspLEI GCGC 1 cut(s) 80
AsuHPI GGTGA 6 cut(s) 56, 245, 469, 812, 902, 1057
AsuII TTCGAA 1 cut(s) 127
AvaI CYCGRG 1 cut(s) 132
AxyI CCTNAGG 1 cut(s) 1070
BalI TGGCCA 1 cut(s) 1059
BbsI GAAGAC 4 cut(s) 116, 645, 768, 1178
BbvCI CCTCAGC 1 cut(s) 482
BbvI GCAGC 2 cut(s) 859, 1063
BccI CCATC 3 cut(s) 319, 647, 727
BciT130I CCWGG 1 cut(s) 1056
BclI TGATCA 1 cut(s) 589
BfaI CTAG 4 cut(s) 75, 459, 905, 1264
BfmI CTRYAG 1 cut(s) 912
BfrI CTTAAG 1 cut(s) 1040
BfuAI ACCTGC 1 cut(s) 308
BisI GCNGC 2 cut(s) 873, 1052
BlsI GCNGC 2 cut(s) 874, 1053
Bme1390I CCNGG 1 cut(s) 1056
BmeT110I CYCGRG 1 cut(s) 132
BmrFI CCNGG 1 cut(s) 1056
BmrI ACTGGG 2 cut(s) 109, 200
BmsI GCATC 5 cut(s) 526, 773, 818, 1171, 1240
BmuI ACTGGG 2 cut(s) 109, 200
BpiI GAAGAC 4 cut(s) 116, 645, 768, 1178
BpmI CTGGAG 1 cut(s) 71
Bpu10I CCTNAGC 1 cut(s) 482
Bpu14I TTCGAA 1 cut(s) 127
BpuEI CTTGAG 2 cut(s) 530, 772
Bsa29I ATCGAT 1 cut(s) 812
BsaJI CCNNGG 2 cut(s) 133, 392
Bsc4I CCNNNNNNNGG 1 cut(s) 139
Bse1I ACTGG 4 cut(s) 88, 115, 195, 1180
Bse21I CCTNAGG 1 cut(s) 1070
Bse3DI GCAATG 1 cut(s) 528
BseBI CCWGG 1 cut(s) 1056
BseCI ATCGAT 1 cut(s) 812
BseDI CCNNGG 2 cut(s) 133, 392
BseGI GGATG 4 cut(s) 355, 379, 619, 640
BseLI CCNNNNNNNGG 1 cut(s) 139
BseMI GCAATG 1 cut(s) 528
BseMII CTCAG 2 cut(s) 312, 496
BseNI ACTGG 4 cut(s) 88, 115, 195, 1180
BseRI GAGGAG 2 cut(s) 644, 752
BseXI GCAGC 2 cut(s) 859, 1063
BshFI GGCC 1 cut(s) 1059
BshVI ATCGAT 1 cut(s) 812
BsiHKCI CYCGRG 1 cut(s) 132
BslI CCNNNNNNNGG 1 cut(s) 139
BsnI GGCC 1 cut(s) 1059
BsoBI CYCGRG 1 cut(s) 132
Bsp119I TTCGAA 1 cut(s) 127
Bsp143I GATC 2 cut(s) 46, 589
BspANI GGCC 1 cut(s) 1059
BspCNI CTCAG 2 cut(s) 313, 495
BspDI ATCGAT 1 cut(s) 812
BspHI TCATGA 1 cut(s) 592
BspMI ACCTGC 1 cut(s) 308
BspT104I TTCGAA 1 cut(s) 127
BspTI CTTAAG 1 cut(s) 1040
BsrDI GCAATG 1 cut(s) 528
BsrI ACTGG 4 cut(s) 88, 115, 195, 1180
BssECI CCNNGG 2 cut(s) 133, 392
BssMI GATC 2 cut(s) 46, 589
BssT1I CCWWGG 1 cut(s) 392
Bst2UI CCWGG 1 cut(s) 1056
Bst6I CTCTTC 3 cut(s) 251, 723, 1076
BstAFI CTTAAG 1 cut(s) 1040
BstBI TTCGAA 1 cut(s) 127
BstC8I GCNNGC 1 cut(s) 1138
BstDEI CTNAG 5 cut(s) 223, 321, 482, 1070, 1112
BstF5I GGATG 4 cut(s) 355, 379, 619, 640
BstHHI GCGC 1 cut(s) 80
BstKTI GATC 2 cut(s) 49, 592
BstMBI GATC 2 cut(s) 46, 589
BstMWI GCNNNNNNNGC 2 cut(s) 316, 989
BstNI CCWGG 1 cut(s) 1056
BstSCI CCNGG 1 cut(s) 1054
BstSFI CTRYAG 1 cut(s) 912
BstV1I GCAGC 2 cut(s) 859, 1063
BstV2I GAAGAC 4 cut(s) 116, 645, 768, 1178
Bsu15I ATCGAT 1 cut(s) 812
Bsu36I CCTNAGG 1 cut(s) 1070
BsuRI GGCC 1 cut(s) 1059
BsuTUI ATCGAT 1 cut(s) 812
BtgZI GCGATG 1 cut(s) 1194
BtsCI GGATG 4 cut(s) 355, 379, 619, 640
BtsIMutI CAGTG 2 cut(s) 95, 122
BveI ACCTGC 1 cut(s) 308
Cac8I GCNNGC 1 cut(s) 1138
CaiI CAGNNNCTG 1 cut(s) 1253
CciI TCATGA 1 cut(s) 592
CfoI GCGC 1 cut(s) 80
ClaI ATCGAT 1 cut(s) 812
Csp6I GTAC 1 cut(s) 107
CspCI CAANNNNNGTGG 2 cut(s) 777, 812
CviAII CATG 3 cut(s) 275, 593, 1017
CviQI GTAC 1 cut(s) 107
DdeI CTNAG 5 cut(s) 223, 321, 482, 1070, 1112
DpnI GATC 2 cut(s) 48, 591
DpnII GATC 2 cut(s) 46, 589
EaeI YGGCCR 1 cut(s) 1057
Eam1104I CTCTTC 3 cut(s) 251, 723, 1076
EarI CTCTTC 3 cut(s) 251, 723, 1076
Eco130I CCWWGG 1 cut(s) 392
Eco32I GATATC 3 cut(s) 357, 367, 475
Eco57I CTGAAG 2 cut(s) 23, 741
Eco81I CCTNAGG 1 cut(s) 1070
Eco88I CYCGRG 1 cut(s) 132
EcoRII CCWGG 1 cut(s) 1054
EcoRV GATATC 3 cut(s) 357, 367, 475
EcoT14I CCWWGG 1 cut(s) 392
ErhI CCWWGG 1 cut(s) 392
FaeI CATG 3 cut(s) 278, 596, 1020
FatI CATG 3 cut(s) 274, 592, 1016
FauNDI CATATG 1 cut(s) 988
FbaI TGATCA 1 cut(s) 589
FblI GTMKAC 2 cut(s) 241, 954
Fnu4HI GCNGC 2 cut(s) 873, 1052
FokI GGATG 4 cut(s) 362, 386, 626, 647
Fsp4HI GCNGC 2 cut(s) 873, 1052
FspBI CTAG 4 cut(s) 75, 459, 905, 1264
GlaI GCGC 1 cut(s) 79
GluI GCNGC 2 cut(s) 873, 1052
GsuI CTGGAG 1 cut(s) 71
HaeIII GGCC 1 cut(s) 1059
HhaI GCGC 1 cut(s) 80
Hin1II CATG 3 cut(s) 278, 596, 1020
Hin6I GCGC 1 cut(s) 78
HinP1I GCGC 1 cut(s) 78
HincII GTYRAC 1 cut(s) 955
HindII GTYRAC 1 cut(s) 955
HinfI GANTC 5 cut(s) 581, 752, 773, 814, 901
HphI GGTGA 6 cut(s) 56, 245, 469, 812, 902, 1057
Hpy166II GTNNAC 3 cut(s) 236, 242, 955
Hpy188I TCNGA 2 cut(s) 556, 574
Hpy188III TCNNGA 6 cut(s) 347, 362, 509, 593, 780, 789
Hpy8I GTNNAC 3 cut(s) 236, 242, 955
HpyAV CCTTC 2 cut(s) 120, 157
HpyCH4IV ACGT 1 cut(s) 238
HpyCH4V TGCA 6 cut(s) 211, 303, 539, 1020, 1154, 1232
HpyF10VI GCNNNNNNNGC 2 cut(s) 316, 989
HpyF3I CTNAG 5 cut(s) 223, 321, 482, 1070, 1112
HpySE526I ACGT 1 cut(s) 238
Hsp92II CATG 3 cut(s) 278, 596, 1020
HspAI GCGC 1 cut(s) 78
Ksp22I TGATCA 1 cut(s) 589
Kzo9I GATC 2 cut(s) 46, 589
LmnI GCTCC 1 cut(s) 908
Lsp1109I GCAGC 2 cut(s) 859, 1063
LweI GCATC 5 cut(s) 526, 773, 818, 1171, 1240
MaeI CTAG 4 cut(s) 75, 459, 905, 1264
MaeII ACGT 1 cut(s) 238
MalI GATC 2 cut(s) 48, 591
MboI GATC 2 cut(s) 46, 589
MlsI TGGCCA 1 cut(s) 1059
MluCI AATT 8 cut(s) 269, 803, 946, 1105, 1148, 1165, 1214, 1233
MluNI TGGCCA 1 cut(s) 1059
MlyI GAGTC 1 cut(s) 782
MmeI TCCRAC 3 cut(s) 125, 176, 211
MnlI CCTC 9 cut(s) 128, 444, 491, 604, 622, 625, 730, 733, 886
Mox20I TGGCCA 1 cut(s) 1059
MroXI GAANNNNTTC 2 cut(s) 838, 900
MscI TGGCCA 1 cut(s) 1059
MseI TTAA 3 cut(s) 96, 168, 1041
MslI CAYNNNNRTG 1 cut(s) 348
Msp20I TGGCCA 1 cut(s) 1059
MspCI CTTAAG 1 cut(s) 1040
MspR9I CCNGG 1 cut(s) 1056
MvaI CCWGG 1 cut(s) 1056
MwoI GCNNNNNNNGC 2 cut(s) 316, 989
NdeI CATATG 1 cut(s) 988
NdeII GATC 2 cut(s) 46, 589
NlaIII CATG 3 cut(s) 278, 596, 1020
NmeAIII GCCGAG 1 cut(s) 1054
NspV TTCGAA 1 cut(s) 127
PagI TCATGA 1 cut(s) 592
PdmI GAANNNNTTC 2 cut(s) 838, 900
PfeI GAWTC 4 cut(s) 581, 752, 814, 901
PkrI GCNGC 2 cut(s) 874, 1053
PleI GAGTC 1 cut(s) 781
PpsI GAGTC 1 cut(s) 781
Psp6I CCWGG 1 cut(s) 1054
PspGI CCWGG 1 cut(s) 1054
PstNI CAGNNNCTG 1 cut(s) 1253
RsaI GTAC 1 cut(s) 108
RsaNI GTAC 1 cut(s) 107
RseI CAYNNNNRTG 1 cut(s) 348
SalI GTCGAC 1 cut(s) 953
SaqAI TTAA 3 cut(s) 96, 168, 1041
SatI GCNGC 2 cut(s) 873, 1052
Sau3AI GATC 2 cut(s) 46, 589
SchI GAGTC 1 cut(s) 782
ScrFI CCNGG 1 cut(s) 1056
SfaNI GCATC 5 cut(s) 526, 773, 818, 1171, 1240
SfcI CTRYAG 1 cut(s) 912
SfuI TTCGAA 1 cut(s) 127
SmiMI CAYNNNNRTG 1 cut(s) 348
SmlI CTYRAG 3 cut(s) 509, 787, 1040
SmoI CTYRAG 3 cut(s) 509, 787, 1040
Sse9I AATT 8 cut(s) 269, 803, 946, 1105, 1148, 1165, 1214, 1233
SspMI CTAG 4 cut(s) 75, 459, 905, 1264
StyD4I CCNGG 1 cut(s) 1054
StyI CCWWGG 1 cut(s) 392
TaiI ACGT 1 cut(s) 241
TaqI TCGA 4 cut(s) 127, 249, 812, 954
TasI AATT 8 cut(s) 269, 803, 946, 1105, 1148, 1165, 1214, 1233
TfiI GAWTC 4 cut(s) 581, 752, 814, 901
Tru1I TTAA 3 cut(s) 96, 168, 1041
Tru9I TTAA 3 cut(s) 96, 168, 1041
TscAI CASTG 2 cut(s) 95, 122
TseI GCWGC 2 cut(s) 872, 1051
TspDTI ATGAA 7 cut(s) 354, 539, 611, 651, 663, 675, 741
TspRI CASTG 2 cut(s) 95, 122
Vha464I CTTAAG 1 cut(s) 1040
XapI RAATTY 2 cut(s) 1148, 1214
XcmI CCANNNNNNNNNTGG 1 cut(s) 95
XmiI GTMKAC 2 cut(s) 241, 954
XmnI GAANNNNTTC 2 cut(s) 838, 900
XspI CTAG 4 cut(s) 75, 459, 905, 1264
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.