Rh2BG170100

B3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0000593
Physical Location & Seq
Reverse (-)
14996517 .. 14999111
2595 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG170100.1

Sequence Viewer

Length: 936 bp
ATGGCAACAGAAAGAAACCCCTCGTTTTTCAAGGTTCTTCAGGGTGATCAGTTCTCTATCAAATTGAAAATCCCTAGAGCATTTCTCCAGTATTTTAATGGAAAGGTACCTCCCAAGTGCTTTCTTCAAACCCTAGGTCAAACTTGGCTTGTTGATGTGGAAAAGAAAAACAAAACAAGTTCTATTGGTAGAGCAGATATCACCTCTAACTTTAGAAAGAGGGTTCCACATCTTGAGAAGTCACAAATGAAAAGGAAAAAGGCACATTGGTTCAAATTAGAAATGATAGATAATTCTGATACTGATTCTGGTGATGAAGATGATCATGATGAGGAGGATGACGACGAAAATGAAGATGATGAAGAAGAAGAAGAAGAAACTCAAGATGAAGAGGTTGAGGAGGAAGATGATGGTGATTCTGTTGGAACCCTCAACGATTCTCTACCATGCTCAAGAACAGTGGGGAAATATACATTAGCATCGAGGCTGCACCAGAGAGAGAGAAAGATTTCTAGCGCAAAAGCAAAAACCAACATCAAAACTGTTGATGGCTTGTCTAGAGAGGAAAAGATCCTAAAAGTTATAGCTCTACAAAGAGCTAATGCTTTCAAATCTAATTCTGTCAACCCTTGTTTCATGGTTTCCTTGTCTGCCACATATGCTCATGGAGCTTTTCTGAATTTGCCACGGAGTTTTGCCGCAGTACATTTTACTAATCCACAGCCTAGCCACGTCATCCTTTGGGTTGAGAAGAGCTCTTGGTTTGTCAAGGTCAGTTATATTGGGACTGCAACGAGATTACAGGCCGGCTGGTCTGAATTTGTGCATGTCAAAAATTTGAAGATTGGTGATGCTTGTGTTTTTGTCTTGACGGATGCCATCAATTTTCAATATGATGTTGAAATATTCCGCGTTACAGATGCTGTTTGCTCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

311

Amino Acids

35.38

Weight (kDa)

5.4

Isoelectric Point (pI)

37.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 223 - 305 3.2e-12 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 106
AccB1I GGYRCC 1 cut(s) 106
AccII CGCG 1 cut(s) 912
AciI CCGC 2 cut(s) 699, 910
AclWI GGATC 1 cut(s) 565
AcsI RAATTY 3 cut(s) 679, 818, 835
AcuI CTGAAG 1 cut(s) 23
AfaI GTAC 2 cut(s) 108, 705
AgsI TTSAA 8 cut(s) 31, 67, 128, 274, 610, 841, 890, 902
AjiI CACGTC 1 cut(s) 733
AluBI AGCT 4 cut(s) 587, 599, 671, 756
AluI AGCT 4 cut(s) 587, 599, 671, 756
Alw21I GWGCWC 1 cut(s) 758
AlwI GGATC 1 cut(s) 565
AlwNI CAGNNNCTG 1 cut(s) 923
AoxI GGCC 1 cut(s) 804
ApeKI GCWGC 1 cut(s) 487
ApoI RAATTY 3 cut(s) 679, 818, 835
ArsI GACNNNNNNTTYG 2 cut(s) 121, 153
Asp700I GAANNNNTTC 1 cut(s) 508
Asp718I GGTACC 1 cut(s) 106
AspA2I CCTAGG 1 cut(s) 133
AspLEI GCGC 1 cut(s) 518
AsuHPI GGTGA 5 cut(s) 56, 193, 323, 425, 860
AvrII CCTAGG 1 cut(s) 133
BanI GGYRCC 1 cut(s) 106
BanII GRGCYC 1 cut(s) 758
Bbv12I GWGCWC 1 cut(s) 758
BbvI GCAGC 1 cut(s) 474
BccI CCATC 3 cut(s) 404, 542, 887
BclI TGATCA 2 cut(s) 46, 322
BfaI CTAG 6 cut(s) 75, 134, 513, 558, 726, 934
BisI GCNGC 2 cut(s) 488, 699
BlnI CCTAGG 1 cut(s) 133
BlsI GCNGC 2 cut(s) 489, 700
BmgBI CACGTC 1 cut(s) 733
BmiI GGNNCC 3 cut(s) 108, 225, 427
BmsI GCATC 4 cut(s) 488, 841, 865, 910
BplI GAGNNNNNCTC 4 cut(s) 69, 101, 740, 772
BpmI CTGGAG 1 cut(s) 71
BpuEI CTTGAG 3 cut(s) 254, 366, 436
BsaJI CCNNGG 2 cut(s) 133, 686
Bse118I RCCGGY 1 cut(s) 806
Bse1I ACTGG 1 cut(s) 88
BseDI CCNNGG 2 cut(s) 133, 686
BseGI GGATG 3 cut(s) 343, 735, 880
BseNI ACTGG 1 cut(s) 88
BseRI GAGGAG 2 cut(s) 347, 413
BseXI GCAGC 1 cut(s) 474
BsgI GTGCAG 1 cut(s) 473
Bsh1236I CGCG 1 cut(s) 912
BshFI GGCC 1 cut(s) 806
BshNI GGYRCC 1 cut(s) 106
BsiHKAI GWGCWC 1 cut(s) 758
BsiSI CCGG 1 cut(s) 807
BslFI GGGAC 1 cut(s) 799
BsmFI GGGAC 1 cut(s) 799
BsnI GGCC 1 cut(s) 806
Bsp1286I GDGCHC 1 cut(s) 758
Bsp143I GATC 3 cut(s) 46, 322, 570
BspACI CCGC 2 cut(s) 699, 910
BspANI GGCC 1 cut(s) 806
BspFNI CGCG 1 cut(s) 912
BspHI TCATGA 1 cut(s) 325
BspLI GGNNCC 3 cut(s) 108, 225, 427
BspPI GGATC 1 cut(s) 565
BspQI GCTCTTC 1 cut(s) 746
BspT107I GGYRCC 1 cut(s) 106
BsrFI RCCGGY 1 cut(s) 806
BsrI ACTGG 1 cut(s) 88
BssAI RCCGGY 1 cut(s) 806
BssECI CCNNGG 2 cut(s) 133, 686
BssMI GATC 3 cut(s) 46, 322, 570
BssT1I CCWWGG 1 cut(s) 133
Bst4CI ACNGT 2 cut(s) 460, 544
Bst6I CTCTTC 2 cut(s) 384, 746
BstC8I GCNNGC 1 cut(s) 808
BstDSI CCRYGG 1 cut(s) 686
BstF5I GGATG 3 cut(s) 343, 735, 880
BstFNI CGCG 1 cut(s) 912
BstHHI GCGC 1 cut(s) 518
BstKTI GATC 3 cut(s) 49, 325, 573
BstMBI GATC 3 cut(s) 46, 322, 570
BstMWI GCNNNNNNNGC 2 cut(s) 659, 668
BstNSI RCATGY 1 cut(s) 830
BstUI CGCG 1 cut(s) 912
BstV1I GCAGC 1 cut(s) 474
BstX2I RGATCY 1 cut(s) 570
BstYI RGATCY 1 cut(s) 570
BsuRI GGCC 1 cut(s) 806
BtgI CCRYGG 1 cut(s) 686
BtrI CACGTC 1 cut(s) 733
BtsCI GGATG 3 cut(s) 343, 735, 880
BtsIMutI CAGTG 1 cut(s) 465
Cac8I GCNNGC 1 cut(s) 808
CaiI CAGNNNCTG 1 cut(s) 923
CciI TCATGA 1 cut(s) 325
CfoI GCGC 1 cut(s) 518
Cfr10I RCCGGY 1 cut(s) 806
Csp6I GTAC 2 cut(s) 107, 704
CspCI CAANNNNNGTGG 2 cut(s) 441, 476
CviAII CATG 5 cut(s) 326, 447, 637, 665, 827
CviQI GTAC 2 cut(s) 107, 704
DpnI GATC 3 cut(s) 48, 324, 572
DpnII GATC 3 cut(s) 46, 322, 570
Eam1104I CTCTTC 2 cut(s) 384, 746
EarI CTCTTC 2 cut(s) 384, 746
Ecl136II GAGCTC 1 cut(s) 756
Eco130I CCWWGG 1 cut(s) 133
Eco24I GRGCYC 1 cut(s) 758
Eco32I GATATC 1 cut(s) 199
Eco53kI GAGCTC 1 cut(s) 756
Eco57I CTGAAG 1 cut(s) 23
EcoICRI GAGCTC 1 cut(s) 756
EcoRV GATATC 1 cut(s) 199
EcoT14I CCWWGG 1 cut(s) 133
EcoT38I GRGCYC 1 cut(s) 758
ErhI CCWWGG 1 cut(s) 133
FaeI CATG 5 cut(s) 329, 450, 640, 668, 830
FaqI GGGAC 1 cut(s) 799
FatI CATG 5 cut(s) 325, 446, 636, 664, 826
FauNDI CATATG 1 cut(s) 658
FbaI TGATCA 2 cut(s) 46, 322
Fnu4HI GCNGC 2 cut(s) 488, 699
FokI GGATG 3 cut(s) 350, 722, 887
FriOI GRGCYC 1 cut(s) 758
Fsp4HI GCNGC 2 cut(s) 488, 699
FspBI CTAG 6 cut(s) 75, 134, 513, 558, 726, 934
GlaI GCGC 1 cut(s) 517
GluI GCNGC 2 cut(s) 488, 699
GsuI CTGGAG 1 cut(s) 71
HaeIII GGCC 1 cut(s) 806
HapII CCGG 1 cut(s) 807
HhaI GCGC 1 cut(s) 518
Hin1II CATG 5 cut(s) 329, 450, 640, 668, 830
Hin6I GCGC 1 cut(s) 516
HinP1I GCGC 1 cut(s) 516
HincII GTYRAC 1 cut(s) 625
HindII GTYRAC 1 cut(s) 625
HinfI GANTC 3 cut(s) 305, 416, 437
HpaII CCGG 1 cut(s) 807
HphI GGTGA 5 cut(s) 56, 193, 323, 425, 860
Hpy166II GTNNAC 1 cut(s) 625
Hpy188I TCNGA 3 cut(s) 298, 678, 817
Hpy188III TCNNGA 6 cut(s) 233, 326, 383, 453, 558, 868
Hpy8I GTNNAC 1 cut(s) 625
Hpy99I CGWCG 1 cut(s) 347
HpyCH4III ACNGT 2 cut(s) 460, 544
HpyCH4IV ACGT 1 cut(s) 732
HpyCH4V TGCA 3 cut(s) 490, 791, 826
HpyF10VI GCNNNNNNNGC 2 cut(s) 659, 668
HpySE526I ACGT 1 cut(s) 732
Hsp92II CATG 5 cut(s) 329, 450, 640, 668, 830
HspAI GCGC 1 cut(s) 516
KpnI GGTACC 1 cut(s) 110
KroI GCCGGC 1 cut(s) 806
KroNI GCCGGC 1 cut(s) 808
Ksp22I TGATCA 2 cut(s) 46, 322
Kzo9I GATC 3 cut(s) 46, 322, 570
LguI GCTCTTC 1 cut(s) 746
LmnI GCTCC 2 cut(s) 668, 935
LpnPI CCDG 7 cut(s) 26, 101, 294, 506, 788, 796, 820
Lsp1109I GCAGC 1 cut(s) 474
LweI GCATC 4 cut(s) 488, 841, 865, 910
MaeI CTAG 6 cut(s) 75, 134, 513, 558, 726, 934
MaeII ACGT 1 cut(s) 732
MaeIII GTNAC 2 cut(s) 240, 913
MalI GATC 3 cut(s) 48, 324, 572
MboI GATC 3 cut(s) 46, 322, 570
MflI RGATCY 1 cut(s) 570
MhlI GDGCHC 1 cut(s) 758
MluCI AATT 8 cut(s) 62, 275, 292, 616, 679, 818, 835, 883
MmeI TCCRAC 1 cut(s) 403
MroNI GCCGGC 1 cut(s) 806
MroXI GAANNNNTTC 1 cut(s) 508
MseI TTAA 1 cut(s) 96
MspI CCGG 1 cut(s) 807
MvnI CGCG 1 cut(s) 912
MwoI GCNNNNNNNGC 2 cut(s) 659, 668
NaeI GCCGGC 1 cut(s) 808
NdeI CATATG 1 cut(s) 658
NdeII GATC 3 cut(s) 46, 322, 570
NgoMIV GCCGGC 1 cut(s) 806
NlaIII CATG 5 cut(s) 329, 450, 640, 668, 830
NlaIV GGNNCC 3 cut(s) 108, 225, 427
NmuCI GTSAC 1 cut(s) 240
NspI RCATGY 1 cut(s) 830
PagI TCATGA 1 cut(s) 325
PciSI GCTCTTC 1 cut(s) 746
PdiI GCCGGC 1 cut(s) 808
PdmI GAANNNNTTC 1 cut(s) 508
PfeI GAWTC 3 cut(s) 305, 416, 437
PkrI GCNGC 2 cut(s) 489, 700
Psp124BI GAGCTC 1 cut(s) 758
PspN4I GGNNCC 3 cut(s) 108, 225, 427
PstNI CAGNNNCTG 1 cut(s) 923
PsuI RGATCY 1 cut(s) 570
RsaI GTAC 2 cut(s) 108, 705
RsaNI GTAC 2 cut(s) 107, 704
SacI GAGCTC 1 cut(s) 758
SapI GCTCTTC 1 cut(s) 746
SaqAI TTAA 1 cut(s) 96
SatI GCNGC 2 cut(s) 488, 699
Sau3AI GATC 3 cut(s) 46, 322, 570
SduI GDGCHC 1 cut(s) 758
SfaNI GCATC 4 cut(s) 488, 841, 865, 910
SmlI CTYRAG 3 cut(s) 233, 381, 451
SmoI CTYRAG 3 cut(s) 233, 381, 451
Sse9I AATT 8 cut(s) 62, 275, 292, 616, 679, 818, 835, 883
SsiI CCGC 2 cut(s) 699, 910
SspI AATATT 1 cut(s) 906
SspMI CTAG 6 cut(s) 75, 134, 513, 558, 726, 934
SstI GAGCTC 1 cut(s) 758
StyI CCWWGG 1 cut(s) 133
TaaI ACNGT 2 cut(s) 460, 544
TaiI ACGT 1 cut(s) 735
TaqI TCGA 1 cut(s) 482
TasI AATT 8 cut(s) 62, 275, 292, 616, 679, 818, 835, 883
TatI WGTACW 1 cut(s) 703
TauI GCSGC 1 cut(s) 701
TfiI GAWTC 3 cut(s) 305, 416, 437
Tru1I TTAA 1 cut(s) 96
Tru9I TTAA 1 cut(s) 96
TscAI CASTG 1 cut(s) 465
TseFI GTSAC 1 cut(s) 240
TseI GCWGC 1 cut(s) 487
Tsp45I GTSAC 1 cut(s) 240
TspDTI ATGAA 6 cut(s) 263, 330, 366, 375, 402, 625
TspGWI ACGGA 2 cut(s) 703, 887
TspRI CASTG 1 cut(s) 465
XapI RAATTY 3 cut(s) 679, 818, 835
XbaI TCTAGA 1 cut(s) 557
XceI RCATGY 1 cut(s) 830
XcmI CCANNNNNNNNNTGG 1 cut(s) 95
XmaJI CCTAGG 1 cut(s) 133
XmnI GAANNNNTTC 1 cut(s) 508
XspI CTAG 6 cut(s) 75, 134, 513, 558, 726, 934
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.