Rroxscaffold_2G00124370

B3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000593
Physical Location & Seq
Reverse (-)
58643278 .. 58643940
663 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00124370.1

Sequence Viewer

Length: 528 bp
ATGGAGAAGGGGGAGGAAGATGATTATTTTAGTGAAACCCTCAACTATGTTCCACCAGGCTCAAGAACATTAGCTTCGAGGCTTCACAAGAGAAGGAGAAACAGGACAAGTTCTAGTGCTGAAGCACAAAGCGACATACAAAGTGCTGGTGGCTTGTCTAGAGACAAAAAGATCCTAAACTCTATAGCTCTACAGAAAGCTAGTGCTTTTAAGACTATCCGCCCTTCTTTTGTGGTTTCCCTATGTGCCTCATATGCTGGTGGAAATTATCTGAATTTTCCAAAGAGTTTTGCCGAGACACATCTTACTATGCAGCGCCCTAGTTTCGTCACCCTTTGGGTTAAGAGGAGCTTTTGGTCCGTCAAGATCAATTATATTGCGAGGACAACCGGATTACAAGCTGGTTGGCCTGAAGCTTTGCAAGTCAATGATCTGAAAATGGGCGATGCTTGTGTTGACCATGCCAATGTCATCAAATTTGTATTTGAGGTTGAAATTTTTCGCATGACATGTGCTGTTTGCACCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

175

Amino Acids

19.63

Weight (kDa)

9.39

Isoelectric Point (pI)

57.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 81 - 167 3.7e-07 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 220
AclWI GGATC 1 cut(s) 166
AcsI RAATTY 3 cut(s) 274, 476, 495
AcuI CTGAAG 2 cut(s) 141, 432
AflIII ACRYGT 1 cut(s) 509
AgsI TTSAA 1 cut(s) 494
AjnI CCWGG 1 cut(s) 55
AluBI AGCT 6 cut(s) 74, 188, 200, 351, 401, 416
AluI AGCT 6 cut(s) 74, 188, 200, 351, 401, 416
Alw26I GTCTC 2 cut(s) 156, 290
AlwI GGATC 1 cut(s) 166
AoxI GGCC 1 cut(s) 407
ApeKI GCWGC 1 cut(s) 313
ApoI RAATTY 3 cut(s) 274, 476, 495
Asp700I GAANNNNTTC 1 cut(s) 498
AspLEI GCGC 1 cut(s) 318
AspS9I GGNCC 1 cut(s) 357
AsuHPI GGTGA 1 cut(s) 322
AvaII GGWCC 1 cut(s) 357
BbvI GCAGC 1 cut(s) 325
BciT130I CCWGG 1 cut(s) 57
BcoDI GTCTC 2 cut(s) 156, 290
BfaI CTAG 4 cut(s) 114, 159, 201, 321
BfmI CTRYAG 2 cut(s) 183, 191
BfoI RGCGCY 1 cut(s) 319
BisI GCNGC 1 cut(s) 314
BlsI GCNGC 1 cut(s) 315
Bme1390I CCNGG 1 cut(s) 57
Bme18I GGWCC 1 cut(s) 357
BmgT120I GGNCC 1 cut(s) 357
BmrFI CCNGG 1 cut(s) 57
BmsI GCATC 1 cut(s) 436
BpuEI CTTGAG 1 cut(s) 46
BsaWI WCCGGW 1 cut(s) 389
BseBI CCWGG 1 cut(s) 57
BseRI GAGGAG 1 cut(s) 361
BseXI GCAGC 1 cut(s) 325
BshFI GGCC 1 cut(s) 409
BsiSI CCGG 1 cut(s) 390
BsmAI GTCTC 2 cut(s) 156, 290
BsnI GGCC 1 cut(s) 409
Bsp143I GATC 3 cut(s) 171, 366, 430
BspACI CCGC 1 cut(s) 220
BspANI GGCC 1 cut(s) 409
BspPI GGATC 1 cut(s) 166
BssMI GATC 3 cut(s) 171, 366, 430
Bst2UI CCWGG 1 cut(s) 57
BstH2I RGCGCY 1 cut(s) 319
BstHHI GCGC 1 cut(s) 318
BstKTI GATC 3 cut(s) 174, 369, 433
BstMAI GTCTC 2 cut(s) 156, 290
BstMBI GATC 3 cut(s) 171, 366, 430
BstMWI GCNNNNNNNGC 1 cut(s) 254
BstNI CCWGG 1 cut(s) 57
BstNSI RCATGY 1 cut(s) 513
BstSCI CCNGG 1 cut(s) 55
BstSFI CTRYAG 2 cut(s) 183, 191
BstV1I GCAGC 1 cut(s) 325
BstX2I RGATCY 1 cut(s) 171
BstYI RGATCY 1 cut(s) 171
BsuRI GGCC 1 cut(s) 409
BtgZI GCGATG 1 cut(s) 459
CfoI GCGC 1 cut(s) 318
Cfr13I GGNCC 1 cut(s) 357
CviAII CATG 3 cut(s) 461, 505, 510
DpnI GATC 3 cut(s) 173, 368, 432
DpnII GATC 3 cut(s) 171, 366, 430
EciI GGCGGA 1 cut(s) 209
Eco47I GGWCC 1 cut(s) 357
Eco57I CTGAAG 2 cut(s) 141, 432
EcoRII CCWGG 1 cut(s) 55
FaeI CATG 3 cut(s) 464, 508, 513
FalI AAGNNNNNCTT 2 cut(s) 335, 367
FatI CATG 3 cut(s) 460, 504, 509
FauNDI CATATG 1 cut(s) 253
Fnu4HI GCNGC 1 cut(s) 314
Fsp4HI GCNGC 1 cut(s) 314
FspBI CTAG 4 cut(s) 114, 159, 201, 321
GlaI GCGC 1 cut(s) 317
GluI GCNGC 1 cut(s) 314
HaeII RGCGCY 1 cut(s) 319
HaeIII GGCC 1 cut(s) 409
HapII CCGG 1 cut(s) 390
HhaI GCGC 1 cut(s) 318
Hin1II CATG 3 cut(s) 464, 508, 513
Hin6I GCGC 1 cut(s) 316
HinP1I GCGC 1 cut(s) 316
HincII GTYRAC 1 cut(s) 457
HindII GTYRAC 1 cut(s) 457
HindIII AAGCTT 1 cut(s) 414
HpaII CCGG 1 cut(s) 390
HphI GGTGA 1 cut(s) 322
Hpy166II GTNNAC 1 cut(s) 457
Hpy188I TCNGA 2 cut(s) 273, 435
Hpy188III TCNNGA 3 cut(s) 63, 159, 364
Hpy8I GTNNAC 1 cut(s) 457
HpyAV CCTTC 2 cut(s) 87, 234
HpyCH4V TGCA 3 cut(s) 313, 421, 522
HpyF10VI GCNNNNNNNGC 1 cut(s) 254
Hsp92II CATG 3 cut(s) 464, 508, 513
HspAI GCGC 1 cut(s) 316
Kzo9I GATC 3 cut(s) 171, 366, 430
LmnI GCTCC 1 cut(s) 348
LpnPI CCDG 8 cut(s) 42, 69, 88, 132, 243, 387, 403, 423
Lsp1109I GCAGC 1 cut(s) 325
LweI GCATC 1 cut(s) 436
MaeI CTAG 4 cut(s) 114, 159, 201, 321
MaeIII GTNAC 1 cut(s) 328
MalI GATC 3 cut(s) 173, 368, 432
MboI GATC 3 cut(s) 171, 366, 430
MboII GAAGA 1 cut(s) 29
MflI RGATCY 1 cut(s) 171
MluCI AATT 5 cut(s) 265, 274, 370, 476, 495
MnlI CCTC 7 cut(s) 7, 50, 72, 259, 339, 375, 481
MroXI GAANNNNTTC 1 cut(s) 498
MseI TTAA 2 cut(s) 210, 342
MslI CAYNNNNRTG 1 cut(s) 465
MspI CCGG 1 cut(s) 390
MspR9I CCNGG 1 cut(s) 57
MvaI CCWGG 1 cut(s) 57
MwoI GCNNNNNNNGC 1 cut(s) 254
NdeI CATATG 1 cut(s) 253
NdeII GATC 3 cut(s) 171, 366, 430
NlaIII CATG 3 cut(s) 464, 508, 513
NmeAIII GCCGAG 1 cut(s) 319
NmuCI GTSAC 1 cut(s) 328
NspI RCATGY 1 cut(s) 513
PciI ACATGT 1 cut(s) 509
PdmI GAANNNNTTC 1 cut(s) 498
PkrI GCNGC 1 cut(s) 315
PscI ACATGT 1 cut(s) 509
Psp6I CCWGG 1 cut(s) 55
PspGI CCWGG 1 cut(s) 55
PspPI GGNCC 1 cut(s) 357
PsuI RGATCY 1 cut(s) 171
RseI CAYNNNNRTG 1 cut(s) 465
SaqAI TTAA 2 cut(s) 210, 342
SatI GCNGC 1 cut(s) 314
Sau3AI GATC 3 cut(s) 171, 366, 430
Sau96I GGNCC 1 cut(s) 357
ScrFI CCNGG 1 cut(s) 57
SetI ASST 8 cut(s) 76, 190, 202, 353, 403, 418, 492, 527
SfaNI GCATC 1 cut(s) 436
SfcI CTRYAG 2 cut(s) 183, 191
SinI GGWCC 1 cut(s) 357
SmiMI CAYNNNNRTG 1 cut(s) 465
SmlI CTYRAG 1 cut(s) 61
SmoI CTYRAG 1 cut(s) 61
Sse9I AATT 5 cut(s) 265, 274, 370, 476, 495
SsiI CCGC 1 cut(s) 220
SspMI CTAG 4 cut(s) 114, 159, 201, 321
StyD4I CCNGG 1 cut(s) 55
TaqI TCGA 1 cut(s) 77
TasI AATT 5 cut(s) 265, 274, 370, 476, 495
Tru1I TTAA 2 cut(s) 210, 342
Tru9I TTAA 2 cut(s) 210, 342
TseFI GTSAC 1 cut(s) 328
TseI GCWGC 1 cut(s) 313
Tsp45I GTSAC 1 cut(s) 328
TspGWI ACGGA 1 cut(s) 349
VpaK11BI GGWCC 1 cut(s) 357
XapI RAATTY 3 cut(s) 274, 476, 495
XbaI TCTAGA 1 cut(s) 158
XceI RCATGY 1 cut(s) 513
XmnI GAANNNNTTC 1 cut(s) 498
XspI CTAG 4 cut(s) 114, 159, 201, 321
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.