FvH4_5g19930

B3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0000593
Physical Location & Seq
Forward (+)
11740716 .. 11743306
2591 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g19930.t1

Sequence Viewer

Length: 999 bp
ATGGCAACAAAGATAAACCCCTCTTTTATCAAGGCTCTTCAGGGTGATCAGTTCTCTGTCAAGTTGAAACTCCCTAGTGCATTTCTCCAGTGTCTTAATGGAAGGTTACCTCCCCAGTGCAGTCTTCAGACCCAAGGTCGAACTTGGAATGTTTCTGTGGAGAAGGTTAATCACAAGTTCTATTTCCAACTGGGTTGGAATGGCTTTGTGCAAGACAATGGCTTAAAGTGTGGTGAAATTCTAGTCTTCCATTATGCTGGTAACTCACAATTCCATGTGGAAATATTTGGAATAAATACCTGCAAAAAGCTGCATGTAATGCCTGAGATGGAAGAAGCTAATGAACATGATGTATCTCCTGAAATCTTGGACGACCTTCCACCTTGTCCAAGGAAAGCTGAGGAGAAATCTTTCCTTTCAAGCCCTCTGCCTCACAAGAGAAGCAAAGCTAGTTCTAGTGGTAAAGCAGATATCACCCTCAACTTCCTAAAGAGGCTTCCACGTCTTGAGAAGTCAGCAATGAAAAAGAAAGATTCACATCGGTCCGAATCAAAAATGATAAGCATTTTTGATAATGATTCTGGTGATGAAGATCATCATGATGAGAAAGAATATGAGGATGATGATCATGATGAGGAGTGTGATGCCGAAAATGAAGATGATGAAGAAGAAGCAACTGAAGACCAAGAGAAGGAGGAGGATGATGATTCTATTGAAACCCTCAGCGATTCTCCAGGATGCTCAAGAACAGTGAGGAAATATACATTGGCATCAAGTCTTCATCAGAGAAAGAGAAAACGTTATACTGTGAAAGCAAAAACCAAAATCAAAGCTGTTGGTGTTTTGTCCAGTGAGGAAAAGATTCTAAAAGCTATAGCCATACAAAGAGCTTGTACCTTCAAATTTAATTCTGACAACCCTTGTTTCGTGATTTCCATGTCTATATCATATGCTTCTGGAAAATTTCTGGTGAGCTTTCTGTATCCAACACTAAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

333

Amino Acids

37.68

Weight (kDa)

5.73

Isoelectric Point (pI)

59.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 21 - 96 8e-13 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 308
AclI AACGTT 1 cut(s) 799
AcsI RAATTY 3 cut(s) 237, 902, 962
AcuI CTGAAG 3 cut(s) 23, 110, 699
AfaI GTAC 1 cut(s) 895
AfiI CCNNNNNNNGG 1 cut(s) 691
AgsI TTSAA 4 cut(s) 67, 420, 716, 901
AhdI GACNNNNNGTC 1 cut(s) 135
AjiI CACGTC 1 cut(s) 503
AjnI CCWGG 1 cut(s) 733
AjuI GAANNNNNNNTTGG 2 cut(s) 749, 781
AluBI AGCT 8 cut(s) 310, 338, 398, 449, 833, 872, 890, 975
AluI AGCT 8 cut(s) 310, 338, 398, 449, 833, 872, 890, 975
ApeKI GCWGC 1 cut(s) 310
ApoI RAATTY 3 cut(s) 237, 902, 962
Asp700I GAANNNNTTC 2 cut(s) 410, 861
AspS9I GGNCC 1 cut(s) 543
AsuHPI GGTGA 5 cut(s) 56, 245, 466, 596, 982
AvaII GGWCC 1 cut(s) 543
BbsI GAAGAC 4 cut(s) 116, 238, 687, 770
BbvCI CCTCAGC 2 cut(s) 399, 722
BbvI GCAGC 1 cut(s) 297
BccI CCATC 1 cut(s) 322
BciT130I CCWGG 1 cut(s) 735
BciVI GTATCC 1 cut(s) 993
BclI TGATCA 2 cut(s) 46, 625
BfaI CTAG 4 cut(s) 75, 242, 450, 456
BfmI CTRYAG 1 cut(s) 873
BfuAI ACCTGC 1 cut(s) 308
BfuI GTATCC 1 cut(s) 993
BisI GCNGC 1 cut(s) 311
BlsI GCNGC 1 cut(s) 312
Bme1390I CCNGG 1 cut(s) 735
Bme18I GGWCC 1 cut(s) 543
BmeRI GACNNNNNGTC 1 cut(s) 135
BmgBI CACGTC 1 cut(s) 503
BmgT120I GGNCC 1 cut(s) 543
BmrFI CCNGG 1 cut(s) 735
BmrI ACTGGG 2 cut(s) 109, 200
BmsI GCATC 3 cut(s) 634, 728, 779
BmuI ACTGGG 2 cut(s) 109, 200
BpiI GAAGAC 4 cut(s) 116, 238, 687, 770
BpmI CTGGAG 2 cut(s) 71, 717
Bpu10I CCTNAGC 2 cut(s) 399, 722
BpuEI CTTGAG 2 cut(s) 527, 727
BsaBI GATNNNNATC 3 cut(s) 537, 591, 624
BsaJI CCNNGG 2 cut(s) 133, 389
Bsc4I CCNNNNNNNGG 1 cut(s) 691
Bse1I ACTGG 4 cut(s) 88, 115, 195, 849
Bse3DI GCAATG 1 cut(s) 525
Bse8I GATNNNNATC 3 cut(s) 537, 591, 624
BseBI CCWGG 1 cut(s) 735
BseDI CCNNGG 2 cut(s) 133, 389
BseGI GGATG 3 cut(s) 625, 706, 743
BseJI GATNNNNATC 3 cut(s) 537, 591, 624
BseLI CCNNNNNNNGG 1 cut(s) 691
BseMI GCAATG 1 cut(s) 525
BseMII CTCAG 3 cut(s) 315, 390, 736
BseNI ACTGG 4 cut(s) 88, 115, 195, 849
BseRI GAGGAG 3 cut(s) 416, 650, 710
BseXI GCAGC 1 cut(s) 297
BsgI GTGCAG 1 cut(s) 139
BslI CCNNNNNNNGG 1 cut(s) 691
Bsp143I GATC 3 cut(s) 46, 592, 625
BspCNI CTCAG 3 cut(s) 316, 391, 735
BspHI TCATGA 2 cut(s) 598, 628
BspMI ACCTGC 1 cut(s) 308
BspQI GCTCTTC 1 cut(s) 42
BsrDI GCAATG 1 cut(s) 525
BsrI ACTGG 4 cut(s) 88, 115, 195, 849
BssECI CCNNGG 2 cut(s) 133, 389
BssMI GATC 3 cut(s) 46, 592, 625
BssT1I CCWWGG 2 cut(s) 133, 389
Bst2UI CCWGG 1 cut(s) 735
Bst4CI ACNGT 2 cut(s) 751, 808
Bst6I CTCTTC 1 cut(s) 42
BstAPI GCANNNNNTGC 1 cut(s) 319
BstDEI CTNAG 3 cut(s) 324, 399, 722
BstEII GGTNACC 1 cut(s) 105
BstF5I GGATG 3 cut(s) 625, 706, 743
BstKTI GATC 3 cut(s) 49, 595, 628
BstMBI GATC 3 cut(s) 46, 592, 625
BstMWI GCNNNNNNNGC 1 cut(s) 319
BstNI CCWGG 1 cut(s) 735
BstNSI RCATGY 1 cut(s) 317
BstPI GGTNACC 1 cut(s) 105
BstSCI CCNGG 1 cut(s) 733
BstSFI CTRYAG 1 cut(s) 873
BstV1I GCAGC 1 cut(s) 297
BstV2I GAAGAC 4 cut(s) 116, 238, 687, 770
BstXI CCANNNNNNTGG 1 cut(s) 257
BsuI GTATCC 1 cut(s) 993
BtrI CACGTC 1 cut(s) 503
BtsCI GGATG 3 cut(s) 625, 706, 743
BtsIMutI CAGTG 4 cut(s) 95, 122, 756, 856
BveI ACCTGC 1 cut(s) 308
CciI TCATGA 2 cut(s) 598, 628
Cfr13I GGNCC 1 cut(s) 543
CpoI CGGWCCG 1 cut(s) 543
Csp6I GTAC 1 cut(s) 894
CspI CGGWCCG 1 cut(s) 543
CviAII CATG 6 cut(s) 275, 314, 347, 599, 629, 937
CviQI GTAC 1 cut(s) 894
DdeI CTNAG 3 cut(s) 324, 399, 722
DpnI GATC 3 cut(s) 48, 594, 627
DpnII GATC 3 cut(s) 46, 592, 625
DriI GACNNNNNGTC 1 cut(s) 135
Eam1104I CTCTTC 1 cut(s) 42
Eam1105I GACNNNNNGTC 1 cut(s) 135
EarI CTCTTC 1 cut(s) 42
Eco130I CCWWGG 2 cut(s) 133, 389
Eco32I GATATC 1 cut(s) 472
Eco47I GGWCC 1 cut(s) 543
Eco57I CTGAAG 3 cut(s) 23, 110, 699
Eco91I GGTNACC 1 cut(s) 105
EcoO65I GGTNACC 1 cut(s) 105
EcoRII CCWGG 1 cut(s) 733
EcoRV GATATC 1 cut(s) 472
EcoT14I CCWWGG 2 cut(s) 133, 389
ErhI CCWWGG 2 cut(s) 133, 389
FaeI CATG 6 cut(s) 278, 317, 350, 602, 632, 940
FatI CATG 6 cut(s) 274, 313, 346, 598, 628, 936
FauNDI CATATG 1 cut(s) 949
FbaI TGATCA 2 cut(s) 46, 625
Fnu4HI GCNGC 1 cut(s) 311
FokI GGATG 3 cut(s) 632, 713, 750
Fsp4HI GCNGC 1 cut(s) 311
FspBI CTAG 4 cut(s) 75, 242, 450, 456
GluI GCNGC 1 cut(s) 311
GsuI CTGGAG 2 cut(s) 71, 717
Hin1II CATG 6 cut(s) 278, 317, 350, 602, 632, 940
HinfI GANTC 6 cut(s) 533, 548, 578, 707, 728, 862
HphI GGTGA 5 cut(s) 56, 245, 466, 596, 982
Hpy188I TCNGA 4 cut(s) 129, 547, 786, 913
Hpy188III TCNNGA 7 cut(s) 359, 506, 599, 629, 744, 928, 957
HpyAV CCTTC 5 cut(s) 96, 157, 386, 685, 907
HpyCH4III ACNGT 2 cut(s) 751, 808
HpyCH4IV ACGT 2 cut(s) 502, 799
HpyCH4V TGCA 5 cut(s) 80, 120, 211, 303, 313
HpyF10VI GCNNNNNNNGC 1 cut(s) 319
HpyF3I CTNAG 3 cut(s) 324, 399, 722
HpySE526I ACGT 2 cut(s) 502, 799
Hsp92II CATG 6 cut(s) 278, 317, 350, 602, 632, 940
Ksp22I TGATCA 2 cut(s) 46, 625
Kzo9I GATC 3 cut(s) 46, 592, 625
LguI GCTCTTC 1 cut(s) 42
Lsp1109I GCAGC 1 cut(s) 297
LweI GCATC 3 cut(s) 634, 728, 779
MaeI CTAG 4 cut(s) 75, 242, 450, 456
MaeII ACGT 2 cut(s) 502, 799
MaeIII GTNAC 2 cut(s) 105, 260
MalI GATC 3 cut(s) 48, 594, 627
MboI GATC 3 cut(s) 46, 592, 625
MluCI AATT 6 cut(s) 237, 269, 902, 907, 962, 994
MmeI TCCRAC 2 cut(s) 176, 211
MroXI GAANNNNTTC 2 cut(s) 410, 861
MseI TTAA 4 cut(s) 96, 168, 224, 906
MslI CAYNNNNRTG 1 cut(s) 600
MspR9I CCNGG 1 cut(s) 735
MvaI CCWGG 1 cut(s) 735
MwoI GCNNNNNNNGC 1 cut(s) 319
NdeI CATATG 1 cut(s) 949
NdeII GATC 3 cut(s) 46, 592, 625
NlaIII CATG 6 cut(s) 278, 317, 350, 602, 632, 940
NspI RCATGY 1 cut(s) 317
PagI TCATGA 2 cut(s) 598, 628
PciSI GCTCTTC 1 cut(s) 42
PdmI GAANNNNTTC 2 cut(s) 410, 861
PfeI GAWTC 6 cut(s) 533, 548, 578, 707, 728, 862
PfoI TCCNGGA 1 cut(s) 733
PkrI GCNGC 1 cut(s) 312
Psp1406I AACGTT 1 cut(s) 799
Psp6I CCWGG 1 cut(s) 733
PspEI GGTNACC 1 cut(s) 105
PspGI CCWGG 1 cut(s) 733
PspPI GGNCC 1 cut(s) 543
RsaI GTAC 1 cut(s) 895
RsaNI GTAC 1 cut(s) 894
RseI CAYNNNNRTG 1 cut(s) 600
Rsr2I CGGWCCG 1 cut(s) 543
RsrII CGGWCCG 1 cut(s) 543
SapI GCTCTTC 1 cut(s) 42
SaqAI TTAA 4 cut(s) 96, 168, 224, 906
SatI GCNGC 1 cut(s) 311
Sau3AI GATC 3 cut(s) 46, 592, 625
Sau96I GGNCC 1 cut(s) 543
ScrFI CCNGG 1 cut(s) 735
SfaNI GCATC 3 cut(s) 634, 728, 779
SfcI CTRYAG 1 cut(s) 873
SinI GGWCC 1 cut(s) 543
SmiMI CAYNNNNRTG 1 cut(s) 600
SmlI CTYRAG 2 cut(s) 506, 742
SmoI CTYRAG 2 cut(s) 506, 742
Sse9I AATT 6 cut(s) 237, 269, 902, 907, 962, 994
SspI AATATT 1 cut(s) 285
SspMI CTAG 4 cut(s) 75, 242, 450, 456
StyD4I CCNGG 1 cut(s) 733
StyI CCWWGG 2 cut(s) 133, 389
TaaI ACNGT 2 cut(s) 751, 808
TaiI ACGT 2 cut(s) 505, 802
TaqI TCGA 1 cut(s) 139
TaqII GACCGA 1 cut(s) 531
TasI AATT 6 cut(s) 237, 269, 902, 907, 962, 994
TfiI GAWTC 6 cut(s) 533, 548, 578, 707, 728, 862
Tru1I TTAA 4 cut(s) 96, 168, 224, 906
Tru9I TTAA 4 cut(s) 96, 168, 224, 906
TscAI CASTG 4 cut(s) 95, 122, 756, 856
TseI GCWGC 1 cut(s) 310
TspDTI ATGAA 6 cut(s) 357, 536, 603, 669, 678, 770
TspRI CASTG 4 cut(s) 95, 122, 756, 856
VpaK11BI GGWCC 1 cut(s) 543
XapI RAATTY 3 cut(s) 237, 902, 962
XceI RCATGY 1 cut(s) 317
XcmI CCANNNNNNNNNTGG 1 cut(s) 95
XmnI GAANNNNTTC 2 cut(s) 410, 861
XspI CTAG 4 cut(s) 75, 242, 450, 456
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.